Plant Circular RNA Database

Detail information of AT4G18570_circ_g.2

			
						 General Information
					
						CircRNA Name
						AT4G18570_circ_g.2
					
						ID in PlantcircBase
						ath_circ_031575
					
						Alias
						Ath_circ_FC2698, Ath_circ_FC2699
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr4: 10232842-10234309  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, PcircRNA_finder, circRNA_finder
					
						Parent gene
						
							AT4G18570						
					
						Parent gene annotation
						
							AT4g18560/F28J12_220
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT4G18570_circ_g.1 AT4G18570_circ_g.3 						
					
						Support reads
						5
					
						Tissues
						whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT4G18570.2:3
AT4G18570.1:4

General Information
CircRNA Name	AT4G18570_circ_g.2
ID in PlantcircBase	ath_circ_031575
Alias	Ath_circ_FC2698, Ath_circ_FC2699
Organism	Arabidpsis thaliana
Position	chr4: 10232842-10234309 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, PcircRNA_finder, circRNA_finder
Parent gene	AT4G18570
Parent gene annotation	AT4g18560/F28J12_220
Parent gene strand	+
Alternative splicing	AT4G18570_circ_g.1 AT4G18570_circ_g.3
Support reads	5
Tissues	whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT4G18570.2:3 AT4G18570.1:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GAAGAGAGTATCCGCAGAGCTAGAGGCTATTGAAGGTGGTGGCCCTGAAGAGGAAGAGCTAATT
GTCCAAGGTGTTCGATTCGCATTCCGTGTTCATCAGataaaaaccgacgtagaaacgcaaggag
atttcataaggttcttgatcaaagaagtcggaaacgctgcgttttcagacattgaagacgttgt
tcctttcg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 320
Transcript exons: 10232842-10232973,10233390-10233436,10234169-10234309
ATAAAAACCGACGTAGAAACGCAAGGAGATTTCATAAGGTTCTTGATCAAAGAAGTCGGAAACG
CTGCGTTTTCAGACATTGAAGACGTTGTTCCTTTCGTCAAATGGCTCGACGACGAACTCTCTTA
CCTGTCCAGATTCCGGTTGATTGGATGCTCGAAACCGGTATTACTAGCCAGCAGATTAAACTGG
CATCCGTGAAACTAGCGATGAAGTATATGAAGAGAGTATCCGCAGAGCTAGAGGCTATTGAAGG
TGGTGGCCCTGAAGAGGAAGAGCTAATTGTCCAAGGTGTTCGATTCGCATTCCGTGTTCATCAG
Transcript length: 564
Transcript exons: 10232842-10232973,10233058-10233239,10233325-10233436,10
234172-10234309
ATAAAAACCGACGTAGAAACGCAAGGAGATTTCATAAGGTTCTTGATCAAAGAAGTCGGAAACG
CTGCGTTTTCAGACATTGAAGACGTTGTTCCTTTCGTCAAATGGCTCGACGACGAACTCTCTTA
CCTGGTTGATGAGAGAGCAGTGTTGAAACATTTCGAGTGGCCGGAGCAAAAAGCTGATGCTTTA
CGTGAAGCAGCGTTTTGTTATTTCGATCTAAAGAAACTCATATCAGAAGCTTCTCGTTTCCGGG
AAGATCCTCGTCAATCTTCTAGCTCTGCTCTTAAGAAAATGCAAGCTTTGTTTGAAAAGTTAGA
GCATGGTGTTTATAGTCTTTCGAGGATGAGAGAATCGGCGGCAACAAAATTCAAGAGTTTCCAG
ATTCCGGTTGATTGGATGCTCGAAACCGGTATTACTAGCCAGATTAAACTGGCATCCGTGAAAC
TAGCGATGAAGTATATGAAGAGAGTATCCGCAGAGCTAGAGGCTATTGAAGGTGGTGGCCCTGA
AGAGGAAGAGCTAATTGTCCAAGGTGTTCGATTCGCATTCCGTGTTCATCAG
						
					

						Genomic sequence
						
							ATAAAAACCGACGTAGAAACGCAAGGAGATTTCATAAGGTTCTTGATCAAAGAAGTCGGAAACG
CTGCGTTTTCAGACATTGAAGACGTTGTTCCTTTCGTCAAATGGCTCGACGACGAACTCTCTTA
CCTGGTATGAATCAAACTTTCAAATTTTCCCAAAGTTTCTGGAATTATTTAGTTCAAAGTTTGT
AAATTTGTTGTTGTATCTTTGAAGGTTGATGAGAGAGCAGTGTTGAAACATTTCGAGTGGCCGG
AGCAAAAAGCTGATGCTTTACGTGAAGCAGCGTTTTGTTATTTCGATCTAAAGAAACTCATATC
AGAAGCTTCTCGTTTCCGGGAAGATCCTCGTCAATCTTCTAGCTCTGCTCTTAAGAAAATGCAA
GCTTTGTTTGAAAAGTAAATAAAAAAAAATCCATTCTTTGATTCATTTCAAATTCAGATTCTTT
AGATAACTAATGAAGATCTTTTGTTGATTCCAAAGGTTAGAGCATGGTGTTTATAGTCTTTCGA
GGATGAGAGAATCGGCGGCAACAAAATTCAAGAGTTTCCAGATTCCGGTTGATTGGATGCTCGA
AACCGGTATTACTAGCCAGGTCAGCAAACTAATGATTCTCGAGATTTACAATTTTACAATCATA
ATTTATCTCGAGATTTACATTATTGATCCTTCTTCTGTGTTTCTTGCAAGATTTAAATATTGGT
TTTGCTTAATGTTTTTATGATGAATACTTAGTTCCCACTTTTTGGTGCAATTATTGCATATTGA
TTGGATGAAAAAAATACATTTTGGAATATAGAAAACATAGAGGGATAGACAAGAACAACAATTC
TGATGGTCCCCAATTTTATGATAAAAGTCAATTGTTGATGGCAGGACATTTGTAAAATTGTTTT
TTGATACTGTTCTTCACTCTATTATTGTTTTTTTGCTTGTTTACATGGTAAAACTATTATGTAC
TACATGACAAATTCAGATATATACAAATATTTAGCAAAAGTAAACTTGAATACATTGTTTACTT
TAATGTTTTGTGGGGTTTTCCTATGTGCACGCCTGTGAATTTTCGAGATTTTCTCTATTGTATC
TTCTTGTCTGTTTCTTTTGTTGTTGCTTTGGGATTATAATAGACAAATCAATAGGGAATGGAAT
CAGATTCTCTGAAGACTTTTTATGCCTTCATTGTCACTCTTTGAGTCTTAATGTTTAGTGTAGT
GTGCTCAAATTGGGGTCTGTGCAACTTATAAGTCATGCAAATCTCATATTTTTTGAGGAACTTG
GAATTTGGTAAAGTATGAATGTTTTGAATTGTTGTGGAATGTGATTTCAGATTAAACTGGCATC
CGTGAAACTAGCGATGAAGTATATGAAGAGAGTATCCGCAGAGCTAGAGGCTATTGAAGGTGGT
GGCCCTGAAGAGGAAGAGCTAATTGTCCAAGGTGTTCGATTCGCATTCCGTGTTCATCAG
						
					

			
		

Sequences
Splice junction sequence	GAAGAGAGTATCCGCAGAGCTAGAGGCTATTGAAGGTGGTGGCCCTGAAGAGGAAGAGCTAATT GTCCAAGGTGTTCGATTCGCATTCCGTGTTCATCAGataaaaaccgacgtagaaacgcaaggag atttcataaggttcttgatcaaagaagtcggaaacgctgcgttttcagacattgaagacgttgt tcctttcg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 320 Transcript exons: 10232842-10232973,10233390-10233436,10234169-10234309 ATAAAAACCGACGTAGAAACGCAAGGAGATTTCATAAGGTTCTTGATCAAAGAAGTCGGAAACG CTGCGTTTTCAGACATTGAAGACGTTGTTCCTTTCGTCAAATGGCTCGACGACGAACTCTCTTA CCTGTCCAGATTCCGGTTGATTGGATGCTCGAAACCGGTATTACTAGCCAGCAGATTAAACTGG CATCCGTGAAACTAGCGATGAAGTATATGAAGAGAGTATCCGCAGAGCTAGAGGCTATTGAAGG TGGTGGCCCTGAAGAGGAAGAGCTAATTGTCCAAGGTGTTCGATTCGCATTCCGTGTTCATCAG Transcript length: 564 Transcript exons: 10232842-10232973,10233058-10233239,10233325-10233436,10 234172-10234309 ATAAAAACCGACGTAGAAACGCAAGGAGATTTCATAAGGTTCTTGATCAAAGAAGTCGGAAACG CTGCGTTTTCAGACATTGAAGACGTTGTTCCTTTCGTCAAATGGCTCGACGACGAACTCTCTTA CCTGGTTGATGAGAGAGCAGTGTTGAAACATTTCGAGTGGCCGGAGCAAAAAGCTGATGCTTTA CGTGAAGCAGCGTTTTGTTATTTCGATCTAAAGAAACTCATATCAGAAGCTTCTCGTTTCCGGG AAGATCCTCGTCAATCTTCTAGCTCTGCTCTTAAGAAAATGCAAGCTTTGTTTGAAAAGTTAGA GCATGGTGTTTATAGTCTTTCGAGGATGAGAGAATCGGCGGCAACAAAATTCAAGAGTTTCCAG ATTCCGGTTGATTGGATGCTCGAAACCGGTATTACTAGCCAGATTAAACTGGCATCCGTGAAAC TAGCGATGAAGTATATGAAGAGAGTATCCGCAGAGCTAGAGGCTATTGAAGGTGGTGGCCCTGA AGAGGAAGAGCTAATTGTCCAAGGTGTTCGATTCGCATTCCGTGTTCATCAG
Genomic sequence	ATAAAAACCGACGTAGAAACGCAAGGAGATTTCATAAGGTTCTTGATCAAAGAAGTCGGAAACG CTGCGTTTTCAGACATTGAAGACGTTGTTCCTTTCGTCAAATGGCTCGACGACGAACTCTCTTA CCTGGTATGAATCAAACTTTCAAATTTTCCCAAAGTTTCTGGAATTATTTAGTTCAAAGTTTGT AAATTTGTTGTTGTATCTTTGAAGGTTGATGAGAGAGCAGTGTTGAAACATTTCGAGTGGCCGG AGCAAAAAGCTGATGCTTTACGTGAAGCAGCGTTTTGTTATTTCGATCTAAAGAAACTCATATC AGAAGCTTCTCGTTTCCGGGAAGATCCTCGTCAATCTTCTAGCTCTGCTCTTAAGAAAATGCAA GCTTTGTTTGAAAAGTAAATAAAAAAAAATCCATTCTTTGATTCATTTCAAATTCAGATTCTTT AGATAACTAATGAAGATCTTTTGTTGATTCCAAAGGTTAGAGCATGGTGTTTATAGTCTTTCGA GGATGAGAGAATCGGCGGCAACAAAATTCAAGAGTTTCCAGATTCCGGTTGATTGGATGCTCGA AACCGGTATTACTAGCCAGGTCAGCAAACTAATGATTCTCGAGATTTACAATTTTACAATCATA ATTTATCTCGAGATTTACATTATTGATCCTTCTTCTGTGTTTCTTGCAAGATTTAAATATTGGT TTTGCTTAATGTTTTTATGATGAATACTTAGTTCCCACTTTTTGGTGCAATTATTGCATATTGA TTGGATGAAAAAAATACATTTTGGAATATAGAAAACATAGAGGGATAGACAAGAACAACAATTC TGATGGTCCCCAATTTTATGATAAAAGTCAATTGTTGATGGCAGGACATTTGTAAAATTGTTTT TTGATACTGTTCTTCACTCTATTATTGTTTTTTTGCTTGTTTACATGGTAAAACTATTATGTAC TACATGACAAATTCAGATATATACAAATATTTAGCAAAAGTAAACTTGAATACATTGTTTACTT TAATGTTTTGTGGGGTTTTCCTATGTGCACGCCTGTGAATTTTCGAGATTTTCTCTATTGTATC TTCTTGTCTGTTTCTTTTGTTGTTGCTTTGGGATTATAATAGACAAATCAATAGGGAATGGAAT CAGATTCTCTGAAGACTTTTTATGCCTTCATTGTCACTCTTTGAGTCTTAATGTTTAGTGTAGT GTGCTCAAATTGGGGTCTGTGCAACTTATAAGTCATGCAAATCTCATATTTTTTGAGGAACTTG GAATTTGGTAAAGTATGAATGTTTTGAATTGTTGTGGAATGTGATTTCAGATTAAACTGGCATC CGTGAAACTAGCGATGAAGTATATGAAGAGAGTATCCGCAGAGCTAGAGGCTATTGAAGGTGGT GGCCCTGAAGAGGAAGAGCTAATTGTCCAAGGTGTTCGATTCGCATTCCGTGTTCATCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.488044227

Conservation Information
Conserved circRNAs	NA
PMCS	0.488044227

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							10233220-10234306(+)
						
						Potential amino acid sequence
						
							MQALFEKLEHGVYSLSRMRESAATKFKSFQIPVDWMLETGITSQIKLASVKLAMKYMKRVSAEL
EAIEGGGPEEEELIVQGVRFAFRVHQIKTDVETQGDFIRFLIKEVGNAAFSDIEDVVPFVKWLD
DELSYLVDERAVLKHFEWPEQKADALREAAFCYFDLKKLISEASRFREDPRQSSSSALKKMQAL
FEKLEHGVYSLSRMRESAATKFKSFQIPVDWMLETGITSQIKLASVKLAMKYMKRVSAELEAIE
GGGPEEEELIVQGVRFAFRVHQIKTDVETQGDFIRFLIKEVGNAAFSDIEDVVPFVKWLDDELS
YLVDERAVLKHFEWPEQKADALREAAFCYFDLKKLISEASRFREDPRQSSSSALKKMQALFEKL
EHGVYSLSRMRESAATKFKSFQIPVDWMLETGITSQIKLASVKLAMKYMKRVSAELEAIEGGGP
EEEELIVQGVRFAFRVHQIKTDVETQGDFIRFLIKEVGNAAFSDIEDVVPFVKWLDDELSYLVD
ERAVLKHFEWPEQKADALREAAFCYFDLKKLISEASRFREDPRQSSSSALKKMQALFEKLEHGV
YSLSRMRESAATKFKSFQIPVDWMLETGITSQIKLASVKLAMKYMKRVSAELEAIEGGGPEEEE
LIVQGVRFAFRVHQ(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	10233220-10234306(+)
Potential amino acid sequence	MQALFEKLEHGVYSLSRMRESAATKFKSFQIPVDWMLETGITSQIKLASVKLAMKYMKRVSAEL EAIEGGGPEEEELIVQGVRFAFRVHQIKTDVETQGDFIRFLIKEVGNAAFSDIEDVVPFVKWLD DELSYLVDERAVLKHFEWPEQKADALREAAFCYFDLKKLISEASRFREDPRQSSSSALKKMQAL FEKLEHGVYSLSRMRESAATKFKSFQIPVDWMLETGITSQIKLASVKLAMKYMKRVSAELEAIE GGGPEEEELIVQGVRFAFRVHQIKTDVETQGDFIRFLIKEVGNAAFSDIEDVVPFVKWLDDELS YLVDERAVLKHFEWPEQKADALREAAFCYFDLKKLISEASRFREDPRQSSSSALKKMQALFEKL EHGVYSLSRMRESAATKFKSFQIPVDWMLETGITSQIKLASVKLAMKYMKRVSAELEAIEGGGP EEEELIVQGVRFAFRVHQIKTDVETQGDFIRFLIKEVGNAAFSDIEDVVPFVKWLDDELSYLVD ERAVLKHFEWPEQKADALREAAFCYFDLKKLISEASRFREDPRQSSSSALKKMQALFEKLEHGV YSLSRMRESAATKFKSFQIPVDWMLETGITSQIKLASVKLAMKYMKRVSAELEAIEGGGPEEEE LIVQGVRFAFRVHQ(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017;Chen et al., 2017a

circRNA

Detail information of AT4G18570_circ_g.2

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