Plant Circular RNA Database

Detail information of Os01g0538000_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os01g0538000_circ_g.3
					
						ID in PlantcircBase
						osa_circ_002070
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 19684450-19684887  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os01g0538000						
					
						Parent gene annotation
						
							Conserved hypothetical protein. (Os01t0538000-01);Similar to cDN
A, clone: J065161O13, full insert sequence. (Os01t0538000-02);Si
milar to cDNA, clone: J065161O13, full insert sequence. (Os01t05
38000-03);Similar to cDNA, clone: J065161O13, full insert sequen
ce. (Os01t0538000-04)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os01g0538000_circ_g.1 Os01g0538000_circ_g.2 						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0538000-01:2
Os01t0538000-04:2
Os01t0538000-02:2
Os01t0538000-03:2

General Information
CircRNA Name	Os01g0538000_circ_g.3
ID in PlantcircBase	osa_circ_002070
Alias	NA
Organism	Oryza sativa
Position	chr1: 19684450-19684887 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os01g0538000
Parent gene annotation	Conserved hypothetical protein. (Os01t0538000-01);Similar to cDN A, clone: J065161O13, full insert sequence. (Os01t0538000-02);Si milar to cDNA, clone: J065161O13, full insert sequence. (Os01t05 38000-03);Similar to cDNA, clone: J065161O13, full insert sequen ce. (Os01t0538000-04)
Parent gene strand	+
Alternative splicing	Os01g0538000_circ_g.1 Os01g0538000_circ_g.2
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0538000-01:2 Os01t0538000-04:2 Os01t0538000-02:2 Os01t0538000-03:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GCTGGAGAGACGTCTGAAGGCGGAGTTCAAGAAAGTGAATGAGCAGCAATCTGACTTGGGGGTT
GGATATGCGAACTACCAGTCTCCTTACCAGGCATAAacgatcagattccacatattggacctga
agttcaccacaggaaccgtggagtgcaacaaatgctctacccaaaccctccagtatgtttattt
ttgttctt

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							ACGATCAGATTCCACATATTGGACCTGAAGTTCACCACAGGAACCGTGGAGTGCAACAAATGCT
CTACCCAAACCCTCCAGTATGTTTATTTTTGTTCTTTTTTTTATGGTGAATTTCTCATTTTTTC
TGAATACATTTTTTTTTCTCAGTTCCCGATCGATCCTGATTATGAGTTTGGTACTCCGCAACAA
CGAGTCCCATATCAAGCGGCATACACTACAGATGCAGAGAGAAATGACGCAGTTTCTCGTGACA
AGCGTGCTCAGAGGGCGGTTTGGAATACTAATCTCCGTCTTCTAGAGGTGAAGAAATCCGCCCT
GGAGAAGAAGAAGACTGAGCTGGAGAGACGTCTGAAGGCGGAGTTCAAGAAAGTGAATGAGCAG
CAATCTGACTTGGGGGTTGGATATGCGAACTACCAGTCTCCTTACCAGGCATAA

Sequences
Splice junction sequence	GCTGGAGAGACGTCTGAAGGCGGAGTTCAAGAAAGTGAATGAGCAGCAATCTGACTTGGGGGTT GGATATGCGAACTACCAGTCTCCTTACCAGGCATAAacgatcagattccacatattggacctga agttcaccacaggaaccgtggagtgcaacaaatgctctacccaaaccctccagtatgtttattt ttgttctt
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	ACGATCAGATTCCACATATTGGACCTGAAGTTCACCACAGGAACCGTGGAGTGCAACAAATGCT CTACCCAAACCCTCCAGTATGTTTATTTTTGTTCTTTTTTTTATGGTGAATTTCTCATTTTTTC TGAATACATTTTTTTTTCTCAGTTCCCGATCGATCCTGATTATGAGTTTGGTACTCCGCAACAA CGAGTCCCATATCAAGCGGCATACACTACAGATGCAGAGAGAAATGACGCAGTTTCTCGTGACA AGCGTGCTCAGAGGGCGGTTTGGAATACTAATCTCCGTCTTCTAGAGGTGAAGAAATCCGCCCT GGAGAAGAAGAAGACTGAGCTGGAGAGACGTCTGAAGGCGGAGTTCAAGAAAGTGAATGAGCAG CAATCTGACTTGGGGGTTGGATATGCGAACTACCAGTCTCCTTACCAGGCATAA

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_009958 						
					
						PMCS
						0.134893493

Conservation Information
Conserved circRNAs	osi_circ_009958
PMCS	0.134893493

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							19684827-19684474(+)
19684665-19684749(-)
						
						Potential amino acid sequence
						
							MSSNLTWGLDMRTTSLLTRHKRSDSTYWT*(+)
MPLDMGLVVAEYQTHNQDRSGMEGLGRAFVALHGSCGELQVQYVESDRLCLVRRLVVRISNPQV
RLLLIHFLELRLQTSLQLSLLLLQGGFLHL*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	19684827-19684474(+) 19684665-19684749(-)
Potential amino acid sequence	MSSNLTWGLDMRTTSLLTRHKRSDSTYWT(+) MPLDMGLVVAEYQTHNQDRSGMEGLGRAFVALHGSCGELQVQYVESDRLCLVRRLVVRISNPQV RLLLIHFLELRLQTSLQLSLLLLQGGFLHL(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os01g0538000_circ_g.3

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