Plant Circular RNA Database

Detail information of Os02g0649700_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os02g0649700_circ_g.3
					
						ID in PlantcircBase
						osa_circ_015782
					
						Alias
						Os02circ20101
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 26160805-26161338  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						SMALT, Segemehl
					
						Parent gene
						
							Os02g0649700						
					
						Parent gene annotation
						
							ATPase, AAA-type, core domain containing protein. (Os02t0649700-
01);Similar to Cell division protease ftsH homolog 7, chloroplas
tic. (Os02t0649700-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						2
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0649750-00:2
Os02t0649750-00:2
Os02t0649700-01:2

General Information
CircRNA Name	Os02g0649700_circ_g.3
ID in PlantcircBase	osa_circ_015782
Alias	Os02circ20101
Organism	Oryza sativa
Position	chr2: 26160805-26161338 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	SMALT, Segemehl
Parent gene	Os02g0649700
Parent gene annotation	ATPase, AAA-type, core domain containing protein. (Os02t0649700- 01);Similar to Cell division protease ftsH homolog 7, chloroplas tic. (Os02t0649700-02)
Parent gene strand	+
Alternative splicing	NA
Support reads	2
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0649750-00:2 Os02t0649750-00:2 Os02t0649700-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AGAGTATGGTCTTAATCAGCGCATAGGTCCGATATCGGTGGCAACATTGTCAAATGGTGGGCTA
GATGAATCCGGAGGCTCTCCATGGGGAAGGGATCAGggcattgagaaaaagcatgcaaagctga
agggtaatgaaaaggctgttgttgcacggcatgaagttggtcatgccgttgtgggaactgctgt
agcaaacc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 447
Transcript exons: 26160805-26160930,26161018-26161338
GGCATTGAGAAAAAGCATGCAAAGCTGAAGGGTAATGAAAAGGCTGTTGTTGCACGGCATGAAG
TTGGTCATGCCGTTGTGGGAACTGCTGTAGCAAACCTATTGCCAGGGCAGCCACGTGTGGAGAA
ATTGAGTATATTGCCAAGGTCAGGTGGTGCATTGGGCTTTACATACACCCCTCCCACAACAGAA
GATAGATATTTGCTCTTTGTTGATGAACTGCGTGGACGTCTAGTAACACTTTTGGGTGGAAGGG
CAGCAGAGGAAGTAGTACTGTCAGGGCGTGTTTCTACTGGAGCACTTGATGACATAAGGCGTGC
CACTGACATGGCATACAAGGCTGTAGCAGAGTATGGTCTTAATCAGCGCATAGGTCCGATATCG
GTGGCAACATTGTCAAATGGTGGGCTAGATGAATCCGGAGGCTCTCCATGGGGAAGGGATCAG
						
						Genomic sequence
						
							GGCATTGAGAAAAAGCATGCAAAGCTGAAGGGTAATGAAAAGGCTGTTGTTGCACGGCATGAAG
TTGGTCATGCCGTTGTGGGAACTGCTGTAGCAAACCTATTGCCAGGGCAGCCACGTGTGGAGGT
TTGAACGCAAGCAACTATCGATAGAACAGTTTAAGAAAATGTTCGTCTTGGGCTTACATTTTAG
TGTTGTTCTTTTTTCCACCAGAAATTGAGTATATTGCCAAGGTCAGGTGGTGCATTGGGCTTTA
CATACACCCCTCCCACAACAGAAGATAGATATTTGCTCTTTGTTGATGAACTGCGTGGACGTCT
AGTAACACTTTTGGGTGGAAGGGCAGCAGAGGAAGTAGTACTGTCAGGGCGTGTTTCTACTGGA
GCACTTGATGACATAAGGCGTGCCACTGACATGGCATACAAGGCTGTAGCAGAGTATGGTCTTA
ATCAGCGCATAGGTCCGATATCGGTGGCAACATTGTCAAATGGTGGGCTAGATGAATCCGGAGG
CTCTCCATGGGGAAGGGATCAG

Sequences
Splice junction sequence	AGAGTATGGTCTTAATCAGCGCATAGGTCCGATATCGGTGGCAACATTGTCAAATGGTGGGCTA GATGAATCCGGAGGCTCTCCATGGGGAAGGGATCAGggcattgagaaaaagcatgcaaagctga agggtaatgaaaaggctgttgttgcacggcatgaagttggtcatgccgttgtgggaactgctgt agcaaacc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 447 Transcript exons: 26160805-26160930,26161018-26161338 GGCATTGAGAAAAAGCATGCAAAGCTGAAGGGTAATGAAAAGGCTGTTGTTGCACGGCATGAAG TTGGTCATGCCGTTGTGGGAACTGCTGTAGCAAACCTATTGCCAGGGCAGCCACGTGTGGAGAA ATTGAGTATATTGCCAAGGTCAGGTGGTGCATTGGGCTTTACATACACCCCTCCCACAACAGAA GATAGATATTTGCTCTTTGTTGATGAACTGCGTGGACGTCTAGTAACACTTTTGGGTGGAAGGG CAGCAGAGGAAGTAGTACTGTCAGGGCGTGTTTCTACTGGAGCACTTGATGACATAAGGCGTGC CACTGACATGGCATACAAGGCTGTAGCAGAGTATGGTCTTAATCAGCGCATAGGTCCGATATCG GTGGCAACATTGTCAAATGGTGGGCTAGATGAATCCGGAGGCTCTCCATGGGGAAGGGATCAG
Genomic sequence	GGCATTGAGAAAAAGCATGCAAAGCTGAAGGGTAATGAAAAGGCTGTTGTTGCACGGCATGAAG TTGGTCATGCCGTTGTGGGAACTGCTGTAGCAAACCTATTGCCAGGGCAGCCACGTGTGGAGGT TTGAACGCAAGCAACTATCGATAGAACAGTTTAAGAAAATGTTCGTCTTGGGCTTACATTTTAG TGTTGTTCTTTTTTCCACCAGAAATTGAGTATATTGCCAAGGTCAGGTGGTGCATTGGGCTTTA CATACACCCCTCCCACAACAGAAGATAGATATTTGCTCTTTGTTGATGAACTGCGTGGACGTCT AGTAACACTTTTGGGTGGAAGGGCAGCAGAGGAAGTAGTACTGTCAGGGCGTGTTTCTACTGGA GCACTTGATGACATAAGGCGTGCCACTGACATGGCATACAAGGCTGTAGCAGAGTATGGTCTTA ATCAGCGCATAGGTCCGATATCGGTGGCAACATTGTCAAATGGTGGGCTAGATGAATCCGGAGG CTCTCCATGGGGAAGGGATCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_004146* 						
					
						PMCS
						0.506929463

Conservation Information
Conserved circRNAs	osi_circ_004146*
PMCS	0.506929463

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							26161220-26161335(+)
26161305-26160829(+)
26160878-26161302(-)
26161053-26161252(-)
						
						Potential amino acid sequence
						
							MAYKAVAEYGLNQRIGPISVATLSNGGLDESGGSPWGRDQGIEKKHAKLKGNEKAVVARHEVGH
AVVGTAVANLLPGQPRVEKLSILPRSGGALGFTYTPPTTEDRYLLFVDELRGRLVTLLGGRAAE
EVVLSGRVSTGALDDIRRATDMAYKAVAEYGLNQRIGPISVATLSNGGLDESGGSPWGRDQGIE
KKHAKLKGNEKAVVARHEVGHAVVGTAVANLLPGQPRVEKLSILPRSGGALGFTYTPPTTEDRY
LLFVDELRGRLVTLLGGRAAEEVVLSGRVSTGALDDIRRATDMAYKAVAEYGLNQRIGPISVAT
LSNGGLDESGGSPWGRDQGIEKKHAKLKGNEKAVVARHEVGHAVVGTAVANLLPGQPRVEKLSI
LPRSGGALGFTYTPPTTEDRYLLFVDELRGRLVTLLGGRAAEEVVLSGRVSTGALDDIRRATDM
AYKAVAEYGLNQRIGPISVATLSNGGLDESGGSPWGRDQ(+)
MNPEALHGEGIRALRKSMQS*(+)
MTNFMPCNNSLFITLQLCMLFLNALIPSPWRASGFI*(-)
MHHLTLAIYSISPHVAALAIGLLQQFPQRHDQLHAVQQQPFHYPSALHAFSQCPDPFPMESLRI
HLAHHLTMLPPISDLCAD*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	26161220-26161335(+) 26161305-26160829(+) 26160878-26161302(-) 26161053-26161252(-)
Potential amino acid sequence	MAYKAVAEYGLNQRIGPISVATLSNGGLDESGGSPWGRDQGIEKKHAKLKGNEKAVVARHEVGH AVVGTAVANLLPGQPRVEKLSILPRSGGALGFTYTPPTTEDRYLLFVDELRGRLVTLLGGRAAE EVVLSGRVSTGALDDIRRATDMAYKAVAEYGLNQRIGPISVATLSNGGLDESGGSPWGRDQGIE KKHAKLKGNEKAVVARHEVGHAVVGTAVANLLPGQPRVEKLSILPRSGGALGFTYTPPTTEDRY LLFVDELRGRLVTLLGGRAAEEVVLSGRVSTGALDDIRRATDMAYKAVAEYGLNQRIGPISVAT LSNGGLDESGGSPWGRDQGIEKKHAKLKGNEKAVVARHEVGHAVVGTAVANLLPGQPRVEKLSI LPRSGGALGFTYTPPTTEDRYLLFVDELRGRLVTLLGGRAAEEVVLSGRVSTGALDDIRRATDM AYKAVAEYGLNQRIGPISVATLSNGGLDESGGSPWGRDQ(+) MNPEALHGEGIRALRKSMQS(+) MTNFMPCNNSLFITLQLCMLFLNALIPSPWRASGFI(-) MHHLTLAIYSISPHVAALAIGLLQQFPQRHDQLHAVQQQPFHYPSALHAFSQCPDPFPMESLRI HLAHHLTMLPPISDLCAD*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Lu et al., 2015

circRNA

Detail information of Os02g0649700_circ_g.3

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