Plant Circular RNA Database

Detail information of Solyc05g056060.2_circ_g.1

			
						 General Information
					
						CircRNA Name
						Solyc05g056060.2_circ_g.1
					
						ID in PlantcircBase
						sly_circ_001837
					
						Alias
						5:64607539|64607827, Slcirc081
					
						Organism
						
							Solanum lycopersicum						
					
						Position
						
							chr5: 65461189-65461477  JBrowse»						
					
						Reference genome
						
							SL2.50.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Solyc05g056070.2						
					
						Parent gene annotation
						
							Chlorophyll a-b binding protein, chloroplastic
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						3/32
					
						Tissues
						fruit/leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Solyc05g056060.2.1:1
Solyc05g056060.2.1:1
Solyc05g056070.2.1:1

General Information
CircRNA Name	Solyc05g056060.2_circ_g.1
ID in PlantcircBase	sly_circ_001837
Alias	5:64607539\|64607827, Slcirc081
Organism	Solanum lycopersicum
Position	chr5: 65461189-65461477 JBrowse»
Reference genome	SL2.50.38
Type	e-circRNA
Identification method	CIRI
Parent gene	Solyc05g056070.2
Parent gene annotation	Chlorophyll a-b binding protein, chloroplastic
Parent gene strand	+
Alternative splicing	NA
Support reads	3/32
Tissues	fruit/leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Solyc05g056060.2.1:1 Solyc05g056060.2.1:1 Solyc05g056070.2.1:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCAGAAAAGAAGAAGTACCCTGGTGGGGCTTTTGACCCATTGGGCTACTCTAAAGACCCTGCTA
AATTTGAAGAACTCAAAGTCAAGGAAATCAAGAATGcccggaatcatagtaccagaggcattgg
gcttaggtaattgggtcaaggcccaagaatgggcagccattcctggtggacaagctacatattt
gggccagc

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							CCCGGAATCATAGTACCAGAGGCATTGGGCTTAGGTAATTGGGTCAAGGCCCAAGAATGGGCAG
CCATTCCTGGTGGACAAGCTACATATTTGGGCCAGCCTGTTCCATGGGGAACACTTCCTACTAT
TTTGGCTATTGAATTTCTAGCCATAGCTTTTGTAGAACACCAAAGGAGTATGGAAAAAGATTCA
GAAAAGAAGAAGTACCCTGGTGGGGCTTTTGACCCATTGGGCTACTCTAAAGACCCTGCTAAAT
TTGAAGAACTCAAAGTCAAGGAAATCAAGAATG

Sequences
Splice junction sequence	TCAGAAAAGAAGAAGTACCCTGGTGGGGCTTTTGACCCATTGGGCTACTCTAAAGACCCTGCTA AATTTGAAGAACTCAAAGTCAAGGAAATCAAGAATGcccggaatcatagtaccagaggcattgg gcttaggtaattgggtcaaggcccaagaatgggcagccattcctggtggacaagctacatattt gggccagc
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CCCGGAATCATAGTACCAGAGGCATTGGGCTTAGGTAATTGGGTCAAGGCCCAAGAATGGGCAG CCATTCCTGGTGGACAAGCTACATATTTGGGCCAGCCTGTTCCATGGGGAACACTTCCTACTAT TTTGGCTATTGAATTTCTAGCCATAGCTTTTGTAGAACACCAAAGGAGTATGGAAAAAGATTCA GAAAAGAAGAAGTACCCTGGTGGGGCTTTTGACCCATTGGGCTACTCTAAAGACCCTGCTAAAT TTGAAGAACTCAAAGTCAAGGAAATCAAGAATG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.314298847

Conservation Information
Conserved circRNAs	NA
PMCS	0.314298847

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							65461367-65461223(+)
65461476-65461223(+)
65461342-65461380(-)
						
						Potential amino acid sequence
						
							MEKDSEKKKYPGGAFDPLGYSKDPAKFEELKVKEIKNARNHSTRGIGLR*(+)
MPGIIVPEALGLGNWVKAQEWAAIPGGQATYLGQPVPWGTLPTILAIEFLAIAFVEHQRSMEKD
SEKKKYPGGAFDPLGYSKDPAKFEELKVKEIKNARNHSTRGIGLR*(+)
MARNSIAKIVGSVPHGTGWPKYVACPPGMAAHSWALTQLPKPNASGTMIPGILDFLDFEFFKFS
RVFRVAQWVKSPTRVLLLF*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						fruit ripening

Functional Information
Coding potential	Y
Potential coding position	65461367-65461223(+) 65461476-65461223(+) 65461342-65461380(-)
Potential amino acid sequence	MEKDSEKKKYPGGAFDPLGYSKDPAKFEELKVKEIKNARNHSTRGIGLR(+) MPGIIVPEALGLGNWVKAQEWAAIPGGQATYLGQPVPWGTLPTILAIEFLAIAFVEHQRSMEKD SEKKKYPGGAFDPLGYSKDPAKFEELKVKEIKNARNHSTRGIGLR(+) MARNSIAKIVGSVPHGTGWPKYVACPPGMAAHSWALTQLPKPNASGTMIPGILDFLDFEFFKFS RVFRVAQWVKSPTRVLLLF*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	fruit ripening

			
						 Other Information
					
						References
						Yin et al., 2018; Wang et al., 2018b

circRNA

Detail information of Solyc05g056060.2_circ_g.1

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