Plant Circular RNA Database

Detail information of Zm00001d043986_circ_g.1

			
						 General Information
					
						CircRNA Name
						Zm00001d043986_circ_g.1
					
						ID in PlantcircBase
						zma_circ_007846
					
						Alias
						zma_circ_0001338
					
						Organism
						
							Zea mays						
					
						Position
						
							chr3: 215650941-215652154  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Zm00001d043986						
					
						Parent gene annotation
						
							Pyruvate kinase  
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						NA
					
						Tissues
						leaf, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d043986_T006:5
Zm00001d043986_T005:5
Zm00001d043986_T010:1
Zm00001d043986_T002:5
Zm00001d043986_T003:5
Zm00001d043986_T008:5
Zm00001d043986_T004:5
Zm00001d043986_T001:6
Zm00001d043986_T007:5
Zm00001d043986_T009:4

General Information
CircRNA Name	Zm00001d043986_circ_g.1
ID in PlantcircBase	zma_circ_007846
Alias	zma_circ_0001338
Organism	Zea mays
Position	chr3: 215650941-215652154 JBrowse»
Reference genome	AGPv4.38
Type	u-circRNA
Identification method	find_circ
Parent gene	Zm00001d043986
Parent gene annotation	Pyruvate kinase
Parent gene strand	+
Alternative splicing	NA
Support reads	NA
Tissues	leaf, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d043986_T006:5 Zm00001d043986_T005:5 Zm00001d043986_T010:1 Zm00001d043986_T002:5 Zm00001d043986_T003:5 Zm00001d043986_T008:5 Zm00001d043986_T004:5 Zm00001d043986_T001:6 Zm00001d043986_T007:5 Zm00001d043986_T009:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TTCATCCAACTCCAACCCGAGCAGAAGTTTCAGACATTGCTATAGCTGTCCGAGAGGGTGCTGA
TGCAGTTATGCTTTCAGGAGAAACTGCACATGGGAAgaggaatgatgtcattcttggtcaaatc
aaaaactgaagattctgtgaaatgtgaagttattgacggtggtgaattgaaatctaggcgccat
ctgaatgt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 757
Transcript exons: 215650941-215651075,215651153-215651263,215651347-215651
435,215651457-215651576,215651606-215651731,215651979-21
5652154
GAGGAATGATGTCATTCTTGGTCAAATCAAAAACTGAAGATTCTGTGAAATGTGAAGTTATTGA
CGGTGGTGAATTGAAATCTAGGCGCCATCTGAATGTTCGTGGAAAGAGTGCAACCTTGCCATCA
ATAACTGACAAGGACTGGGACGACATTAAGTTTGGTGTGGATAATCAAGTTGATTACTATGCTG
TTTCCTTTGTGAAAGATGCTCAAGTTGTCCACGAACTCAAGGATTATCTAAAAAGTTGCAATGC
TGACATACATGTTATCGTAAAAATAGAAAGTGCAGACTCCATCCCCAACTTACATTCAATCATC
ACAGCATCTGATGGGTTATATGCTTTGGTCTAGTGAAATGCCATCAAAAGGTTTATCTATTATC
TTTGCATGAATGAACTTGAGTTGTATGGGAAATCATGAATCTAGTATCTGCTTTCCTCATTCTG
ACTTTAGTGTCAATTATTGCTTGTTTGACATTTTGGGGGTAACAGACTAATATAATATCGAACA
CTATAGGCTATGGTTGCCAGGGGTGACCTTGGAGCCGAGCTGCCCATTGAGGAGGTGCCGCTGT
TGCAGGAAGAAATTATTAGAATGTGCAGGAGCATGGGGAAGGCTGTTATTGTTGCTACAAATAT
GCTCGAAAGTATGATTGTTCATCCAACTCCAACCCGAGCAGAAGTTTCAGACATTGCTATAGCT
GTCCGAGAGGGTGCTGATGCAGTTATGCTTTCAGGAGAAACTGCACATGGGAA
						
					

						Genomic sequence
						
							GAGGAATGATGTCATTCTTGGTCAAATCAAAAACTGAAGATTCTGTGAAATGTGAAGTTATTGA
CGGTGGTGAATTGAAATCTAGGCGCCATCTGAATGTTCGTGGAAAGAGTGCAACCTTGCCATCA
ATAACTGGTAATAGTCGGCAACTTTTTTCTGTCTACTGGATCTAATCTATATATCCTTGTTTCA
TACACGTTTCAAATGTACAGACAAGGACTGGGACGACATTAAGTTTGGTGTGGATAATCAAGTT
GATTACTATGCTGTTTCCTTTGTGAAAGATGCTCAAGTTGTCCACGAACTCAAGGATTATCTAA
AAAGTAATCTTCAACCCAACTTTGTAAAAAATCATATCATGCACCTTTTTTTACAATTTACTGA
GCTCATTCTATTCTATTCTCAGGTTGCAATGCTGACATACATGTTATCGTAAAAATAGAAAGTG
CAGACTCCATCCCCAACTTACATTCAATCATCACAGCATCTGATGGGGTAAAAAATATATTATC
TTAATTATATGCTTTGGTCTAGTGAAATGCCATCAAAAGGTTTATCTATTATCTTTGCATGAAT
GAACTTGAGTTGTATGGGAAATCATGAATCTAGTATCTGCTTTCCTCATTCTGACTTTAGTTTA
AGGGCAGGCACATAAATTTCACCACTGTCAATTATTGCTTGTTTGACATTTTGGGGGTAACAGA
CTAATATAATATCGAACACTATAGGCTATGGTTGCCAGGGGTGACCTTGGAGCCGAGCTGCCCA
TTGAGGAGGTGCCGCTGTTGCAGGTATGATGTCGCTGGTCATATTTCTTATAAGTTATTGCACT
GCAAAGCACTAGTATCTCTTCTATTGGATTATTTTGAGTTACTTTATTGTTTTTACATAGGAAT
ATGGCTTATATGTTGCAGTCCCATTTGAAATCTGCTGTGCCTAGTGCTTACTATAAGCTGTCAG
TAAAGAAATATTTTTTCCTTTGCCGATGAAGGTGTTTCAGATTTACATCTTCCTTGAATATTAT
GTTCTTTCCTGCAGGAAGAAATTATTAGAATGTGCAGGAGCATGGGGAAGGCTGTTATTGTTGC
TACAAATATGCTCGAAAGTATGATTGTTCATCCAACTCCAACCCGAGCAGAAGTTTCAGACATT
GCTATAGCTGTCCGAGAGGGTGCTGATGCAGTTATGCTTTCAGGAGAAACTGCACATGGGAA
						
					

			
		

Sequences
Splice junction sequence	TTCATCCAACTCCAACCCGAGCAGAAGTTTCAGACATTGCTATAGCTGTCCGAGAGGGTGCTGA TGCAGTTATGCTTTCAGGAGAAACTGCACATGGGAAgaggaatgatgtcattcttggtcaaatc aaaaactgaagattctgtgaaatgtgaagttattgacggtggtgaattgaaatctaggcgccat ctgaatgt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 757 Transcript exons: 215650941-215651075,215651153-215651263,215651347-215651 435,215651457-215651576,215651606-215651731,215651979-21 5652154 GAGGAATGATGTCATTCTTGGTCAAATCAAAAACTGAAGATTCTGTGAAATGTGAAGTTATTGA CGGTGGTGAATTGAAATCTAGGCGCCATCTGAATGTTCGTGGAAAGAGTGCAACCTTGCCATCA ATAACTGACAAGGACTGGGACGACATTAAGTTTGGTGTGGATAATCAAGTTGATTACTATGCTG TTTCCTTTGTGAAAGATGCTCAAGTTGTCCACGAACTCAAGGATTATCTAAAAAGTTGCAATGC TGACATACATGTTATCGTAAAAATAGAAAGTGCAGACTCCATCCCCAACTTACATTCAATCATC ACAGCATCTGATGGGTTATATGCTTTGGTCTAGTGAAATGCCATCAAAAGGTTTATCTATTATC TTTGCATGAATGAACTTGAGTTGTATGGGAAATCATGAATCTAGTATCTGCTTTCCTCATTCTG ACTTTAGTGTCAATTATTGCTTGTTTGACATTTTGGGGGTAACAGACTAATATAATATCGAACA CTATAGGCTATGGTTGCCAGGGGTGACCTTGGAGCCGAGCTGCCCATTGAGGAGGTGCCGCTGT TGCAGGAAGAAATTATTAGAATGTGCAGGAGCATGGGGAAGGCTGTTATTGTTGCTACAAATAT GCTCGAAAGTATGATTGTTCATCCAACTCCAACCCGAGCAGAAGTTTCAGACATTGCTATAGCT GTCCGAGAGGGTGCTGATGCAGTTATGCTTTCAGGAGAAACTGCACATGGGAA
Genomic sequence	GAGGAATGATGTCATTCTTGGTCAAATCAAAAACTGAAGATTCTGTGAAATGTGAAGTTATTGA CGGTGGTGAATTGAAATCTAGGCGCCATCTGAATGTTCGTGGAAAGAGTGCAACCTTGCCATCA ATAACTGGTAATAGTCGGCAACTTTTTTCTGTCTACTGGATCTAATCTATATATCCTTGTTTCA TACACGTTTCAAATGTACAGACAAGGACTGGGACGACATTAAGTTTGGTGTGGATAATCAAGTT GATTACTATGCTGTTTCCTTTGTGAAAGATGCTCAAGTTGTCCACGAACTCAAGGATTATCTAA AAAGTAATCTTCAACCCAACTTTGTAAAAAATCATATCATGCACCTTTTTTTACAATTTACTGA GCTCATTCTATTCTATTCTCAGGTTGCAATGCTGACATACATGTTATCGTAAAAATAGAAAGTG CAGACTCCATCCCCAACTTACATTCAATCATCACAGCATCTGATGGGGTAAAAAATATATTATC TTAATTATATGCTTTGGTCTAGTGAAATGCCATCAAAAGGTTTATCTATTATCTTTGCATGAAT GAACTTGAGTTGTATGGGAAATCATGAATCTAGTATCTGCTTTCCTCATTCTGACTTTAGTTTA AGGGCAGGCACATAAATTTCACCACTGTCAATTATTGCTTGTTTGACATTTTGGGGGTAACAGA CTAATATAATATCGAACACTATAGGCTATGGTTGCCAGGGGTGACCTTGGAGCCGAGCTGCCCA TTGAGGAGGTGCCGCTGTTGCAGGTATGATGTCGCTGGTCATATTTCTTATAAGTTATTGCACT GCAAAGCACTAGTATCTCTTCTATTGGATTATTTTGAGTTACTTTATTGTTTTTACATAGGAAT ATGGCTTATATGTTGCAGTCCCATTTGAAATCTGCTGTGCCTAGTGCTTACTATAAGCTGTCAG TAAAGAAATATTTTTTCCTTTGCCGATGAAGGTGTTTCAGATTTACATCTTCCTTGAATATTAT GTTCTTTCCTGCAGGAAGAAATTATTAGAATGTGCAGGAGCATGGGGAAGGCTGTTATTGTTGC TACAAATATGCTCGAAAGTATGATTGTTCATCCAACTCCAACCCGAGCAGAAGTTTCAGACATT GCTATAGCTGTCCGAGAGGGTGCTGATGCAGTTATGCTTTCAGGAGAAACTGCACATGGGAA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.154020718

Conservation Information
Conserved circRNAs	NA
PMCS	0.154020718

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							215652119-215651471(+)
215651673-215650974(+)
215651173-215652134(-)
215650957-215652058(-)
						
						Potential amino acid sequence
						
							MQLCFQEKLHMGRGMMSFLVKSKTEDSVKCEVIDGGELKSRRHLNVRGKSATLPSITDKDWDDI
KFGVDNQVDYYAVSFVKDAQVVHELKDYLKSCNADIHVIVKIESADSIPNLHSIITASDGLYAL
V*(+)
MVARGDLGAELPIEEVPLLQEEIIRMCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVRE
GADAVMLSGETAHGKRNDVILGQIKN*(+)
MSSQSLSVIDGKVALFPRTFRWRLDFNSPPSITSHFTESSVFDLTKNDIIPLPMCSFS*(-)
MTSFLFPCAVSPESITASAPSRTAIAMSETSARVGVG*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	215652119-215651471(+) 215651673-215650974(+) 215651173-215652134(-) 215650957-215652058(-)
Potential amino acid sequence	MQLCFQEKLHMGRGMMSFLVKSKTEDSVKCEVIDGGELKSRRHLNVRGKSATLPSITDKDWDDI KFGVDNQVDYYAVSFVKDAQVVHELKDYLKSCNADIHVIVKIESADSIPNLHSIITASDGLYAL V(+) MVARGDLGAELPIEEVPLLQEEIIRMCRSMGKAVIVATNMLESMIVHPTPTRAEVSDIAIAVRE GADAVMLSGETAHGKRNDVILGQIKN(+) MSSQSLSVIDGKVALFPRTFRWRLDFNSPPSITSHFTESSVFDLTKNDIIPLPMCSFS(-) MTSFLFPCAVSPESITASAPSRTAIAMSETSARVGVG(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ma et al., 2021b

circRNA

Detail information of Zm00001d043986_circ_g.1

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