Plant Circular RNA Database

Detail information of Os06g0660700_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os06g0660700_circ_g.2
					
						ID in PlantcircBase
						osa_circ_031848
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr6: 27221296-27222293  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder
					
						Parent gene
						
							Os06g0660700						
					
						Parent gene annotation
						
							Similar to Ubiquitin carrier protein. (Os06t0660700-01);Similar 
to Ubiquitin carrier protein. (Os06t0660700-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						8
					
						Tissues
						shoot, seed
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os06t0660700-02:5
Os06t0660700-01:5

General Information
CircRNA Name	Os06g0660700_circ_g.2
ID in PlantcircBase	osa_circ_031848
Alias	NA
Organism	Oryza sativa
Position	chr6: 27221296-27222293 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, PcircRNA_finder
Parent gene	Os06g0660700
Parent gene annotation	Similar to Ubiquitin carrier protein. (Os06t0660700-01);Similar to Ubiquitin carrier protein. (Os06t0660700-02)
Parent gene strand	+
Alternative splicing	NA
Support reads	8
Tissues	shoot, seed
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os06t0660700-02:5 Os06t0660700-01:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TGAGATGCCTTCTGATAGAACCATTTCCTGAATCTGCCCTTAATGAGCAAGCTGGGAAATTGTT
GCTTGAGAACTATGAGGAGTATGCACGGCATGCAAGgcttgttttggcgattgatcacactgta
gaaatcaaattccaagtgaagttgccaaacaattacctatctgctgttattgcttccatggtaa
gccatcag

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 528
Transcript exons: 27221296-27221383,27221506-27221650,27221802-27221879,27
221975-27222087,27222190-27222293
GCTTGTTTTGGCGATTGATCACACTGTAGAAATCAAATTCCAAGTGAAGTTGCCAAACAATTAC
CTATCTGCTGTTATTGCTTCCATGGCAACCAACGAGAACCTCCCACCAAATGTCGTTAGGCAAT
TGGCTAAGGAACTGAAGAGTCTTGATGAGTCACCTCCAGAAGGGATTAAAGTTATTGTTAATGA
CGATGACTTCACCACCATTTTTGCTGATATTGAGGGACCTGCTGGAACTCCATATGAGAATGGA
GTATTCCGTATGAAGCTATTGTTGTCTCGTGACTTCCCTCAGTCTCCTCCTAAAGGATTCTTCT
TGACAAAAATTTTCCATCCAAATATAGCAACTAGTGGTGAAATATGTGTTAACACACTGAAGAA
GGATTGGAATCCTAGCCTTGGATTAAGACATGTTCTGCTGGTAGTGAGATGCCTTCTGATAGAA
CCATTTCCTGAATCTGCCCTTAATGAGCAAGCTGGGAAATTGTTGCTTGAGAACTATGAGGAGT
ATGCACGGCATGCAAG
						
					

						Genomic sequence
						
							GCTTGTTTTGGCGATTGATCACACTGTAGAAATCAAATTCCAAGTGAAGTTGCCAAACAATTAC
CTATCTGCTGTTATTGCTTCCATGGTAAGCCATCAGAAGTATTTAATAATTTTGGGACCTCATT
CATTTTCAAGAGAACACCACTTCAAAGTAGCTATTCTCTCTTTCATGATTCTAACTTCTAATTG
TATGTCTGCATTACCAAGGCAACCAACGAGAACCTCCCACCAAATGTCGTTAGGCAATTGGCTA
AGGAACTGAAGAGTCTTGATGAGTCACCTCCAGAAGGGATTAAAGTTATTGTTAATGACGATGA
CTTCACCACCATTTTTGCTGATATTGAGGGACCTGGTAATGATATATCAATATATGCTCTTGTG
CTTGCTTTGCACCATTCTAGCACATTGTTCTGCTGCTTGCAGTGCATAATAGAACCCGTATTAT
TTTTCCTGTTGTACTTCTTACACCATATTCTGAAAATCTGAATCTTGACTTGGTGCAGCTGGAA
CTCCATATGAGAATGGAGTATTCCGTATGAAGCTATTGTTGTCTCGTGACTTCCCTCAGTCTCC
TCCTAAAGGTAGCTATCCCTCAAAACTTTCAGTAATGTTCTTCTTTAGTTCTTCAAGTGAGGCA
TTTGGTTGTAAGTCTTTGACCTTCCCTTTTGATTTGCAGGATTCTTCTTGACAAAAATTTTCCA
TCCAAATATAGCAACTAGTGGTGAAATATGTGTTAACACACTGAAGAAGGATTGGAATCCTAGC
CTTGGATTAAGACATGTTCTGCTGGTAACCAGAATCATATTATTCCAGTAATGTTACTTTGTAA
GCAGTTGCTGAGCTATGATTTAGCACAACAGCGAGTAAGAGTTCTTTCTTGGCATGTGACAGGT
AGTGAGATGCCTTCTGATAGAACCATTTCCTGAATCTGCCCTTAATGAGCAAGCTGGGAAATTG
TTGCTTGAGAACTATGAGGAGTATGCACGGCATGCAAG
						
					

			
		

Sequences
Splice junction sequence	TGAGATGCCTTCTGATAGAACCATTTCCTGAATCTGCCCTTAATGAGCAAGCTGGGAAATTGTT GCTTGAGAACTATGAGGAGTATGCACGGCATGCAAGgcttgttttggcgattgatcacactgta gaaatcaaattccaagtgaagttgccaaacaattacctatctgctgttattgcttccatggtaa gccatcag
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 528 Transcript exons: 27221296-27221383,27221506-27221650,27221802-27221879,27 221975-27222087,27222190-27222293 GCTTGTTTTGGCGATTGATCACACTGTAGAAATCAAATTCCAAGTGAAGTTGCCAAACAATTAC CTATCTGCTGTTATTGCTTCCATGGCAACCAACGAGAACCTCCCACCAAATGTCGTTAGGCAAT TGGCTAAGGAACTGAAGAGTCTTGATGAGTCACCTCCAGAAGGGATTAAAGTTATTGTTAATGA CGATGACTTCACCACCATTTTTGCTGATATTGAGGGACCTGCTGGAACTCCATATGAGAATGGA GTATTCCGTATGAAGCTATTGTTGTCTCGTGACTTCCCTCAGTCTCCTCCTAAAGGATTCTTCT TGACAAAAATTTTCCATCCAAATATAGCAACTAGTGGTGAAATATGTGTTAACACACTGAAGAA GGATTGGAATCCTAGCCTTGGATTAAGACATGTTCTGCTGGTAGTGAGATGCCTTCTGATAGAA CCATTTCCTGAATCTGCCCTTAATGAGCAAGCTGGGAAATTGTTGCTTGAGAACTATGAGGAGT ATGCACGGCATGCAAG
Genomic sequence	GCTTGTTTTGGCGATTGATCACACTGTAGAAATCAAATTCCAAGTGAAGTTGCCAAACAATTAC CTATCTGCTGTTATTGCTTCCATGGTAAGCCATCAGAAGTATTTAATAATTTTGGGACCTCATT CATTTTCAAGAGAACACCACTTCAAAGTAGCTATTCTCTCTTTCATGATTCTAACTTCTAATTG TATGTCTGCATTACCAAGGCAACCAACGAGAACCTCCCACCAAATGTCGTTAGGCAATTGGCTA AGGAACTGAAGAGTCTTGATGAGTCACCTCCAGAAGGGATTAAAGTTATTGTTAATGACGATGA CTTCACCACCATTTTTGCTGATATTGAGGGACCTGGTAATGATATATCAATATATGCTCTTGTG CTTGCTTTGCACCATTCTAGCACATTGTTCTGCTGCTTGCAGTGCATAATAGAACCCGTATTAT TTTTCCTGTTGTACTTCTTACACCATATTCTGAAAATCTGAATCTTGACTTGGTGCAGCTGGAA CTCCATATGAGAATGGAGTATTCCGTATGAAGCTATTGTTGTCTCGTGACTTCCCTCAGTCTCC TCCTAAAGGTAGCTATCCCTCAAAACTTTCAGTAATGTTCTTCTTTAGTTCTTCAAGTGAGGCA TTTGGTTGTAAGTCTTTGACCTTCCCTTTTGATTTGCAGGATTCTTCTTGACAAAAATTTTCCA TCCAAATATAGCAACTAGTGGTGAAATATGTGTTAACACACTGAAGAAGGATTGGAATCCTAGC CTTGGATTAAGACATGTTCTGCTGGTAACCAGAATCATATTATTCCAGTAATGTTACTTTGTAA GCAGTTGCTGAGCTATGATTTAGCACAACAGCGAGTAAGAGTTCTTTCTTGGCATGTGACAGGT AGTGAGATGCCTTCTGATAGAACCATTTCCTGAATCTGCCCTTAATGAGCAAGCTGGGAAATTG TTGCTTGAGAACTATGAGGAGTATGCACGGCATGCAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_016512 						
					
						PMCS
						0.297741258

Conservation Information
Conserved circRNAs	osi_circ_016512
PMCS	0.297741258

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							27221382-27222290(+)
27222237-27221321(+)
						
						Potential amino acid sequence
						
							MATNENLPPNVVRQLAKELKSLDESPPEGIKVIVNDDDFTTIFADIEGPAGTPYENGVFRMKLL
LSRDFPQSPPKGFFLTKIFHPNIATSGEICVNTLKKDWNPSLGLRHVLLVVRCLLIEPFPESAL
NEQAGKLLLENYEEYARHARLVLAIDHTVEIKFQVKLPNNYLSAVIASMATNENLPPNVVRQLA
KELKSLDESPPEGIKVIVNDDDFTTIFADIEGPAGTPYENGVFRMKLLLSRDFPQSPPKGFFLT
KIFHPNIATSGEICVNTLKKDWNPSLGLRHVLLVVRCLLIEPFPESALNEQAGKLLLENYEEYA
RHARLVLAIDHTVEIKFQVKLPNNYLSAVIASMATNENLPPNVVRQLAKELKSLDESPPEGIKV
IVNDDDFTTIFADIEGPAGTPYENGVFRMKLLLSRDFPQSPPKGFFLTKIFHPNIATSGEICVN
TLKKDWNPSLGLRHVLLVVRCLLIEPFPESALNEQAGKLLLENYEEYARHARLVLAIDHTVEIK
FQVKLPNNYLSAVIASMATNENLPPNVVRQLAKELKSLDESPPEGIKVIVNDDDFTTIFADIEG
PAGTPYENGVFRMKLLLSRDFPQSPPKGFFLTKIFHPNIATSGEICVNTLKKDWNPSLGLRHVL
LVVRCLLIEPFPESALNEQAGKLLLENYEEYARHA(+)
MSKLGNCCLRTMRSMHGMQGLFWRLITL*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	27221382-27222290(+) 27222237-27221321(+)
Potential amino acid sequence	MATNENLPPNVVRQLAKELKSLDESPPEGIKVIVNDDDFTTIFADIEGPAGTPYENGVFRMKLL LSRDFPQSPPKGFFLTKIFHPNIATSGEICVNTLKKDWNPSLGLRHVLLVVRCLLIEPFPESAL NEQAGKLLLENYEEYARHARLVLAIDHTVEIKFQVKLPNNYLSAVIASMATNENLPPNVVRQLA KELKSLDESPPEGIKVIVNDDDFTTIFADIEGPAGTPYENGVFRMKLLLSRDFPQSPPKGFFLT KIFHPNIATSGEICVNTLKKDWNPSLGLRHVLLVVRCLLIEPFPESALNEQAGKLLLENYEEYA RHARLVLAIDHTVEIKFQVKLPNNYLSAVIASMATNENLPPNVVRQLAKELKSLDESPPEGIKV IVNDDDFTTIFADIEGPAGTPYENGVFRMKLLLSRDFPQSPPKGFFLTKIFHPNIATSGEICVN TLKKDWNPSLGLRHVLLVVRCLLIEPFPESALNEQAGKLLLENYEEYARHARLVLAIDHTVEIK FQVKLPNNYLSAVIASMATNENLPPNVVRQLAKELKSLDESPPEGIKVIVNDDDFTTIFADIEG PAGTPYENGVFRMKLLLSRDFPQSPPKGFFLTKIFHPNIATSGEICVNTLKKDWNPSLGLRHVL LVVRCLLIEPFPESALNEQAGKLLLENYEEYARHA(+) MSKLGNCCLRTMRSMHGMQGLFWRLITL*(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os06g0660700_circ_g.2

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