Plant Circular RNA Database

Detail information of orai.008G284900_circ_g.1

			
						 General Information
					
						CircRNA Name
						orai.008G284900_circ_g.1
					
						ID in PlantcircBase
						gra_circ_000895
					
						Alias
						Chr08:56065236|56067110
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr08: 56065236-56067110  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.008G284900						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						4
					
						Tissues
						ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.008G284900.1:8
Gorai.008G284900.2:8

General Information
CircRNA Name	orai.008G284900_circ_g.1
ID in PlantcircBase	gra_circ_000895
Alias	Chr08:56065236\|56067110
Organism	Gossypium raimondii
Position	chrChr08: 56065236-56067110 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.008G284900
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	NA
Support reads	4
Tissues	ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.008G284900.1:8 Gorai.008G284900.2:8

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TCTTGTTAGGGAATGAAAGATCCACTCTTAGACTCAAAGGCCAAACTAGCCATGCTCAAAGAAC
ATTGTACCGGTCTTGCAATAAGGGAATTGGACGCTGagtacatgaaaggttacgcaatgctgaa
actcgtgaaggggcctcttttacttcccttttcggtcctgctctttgtcttggcatcatatcta
gaaggaga

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 1022
Transcript exons: 56065236-56065449,56065596-56065685,56065822-56066077,56
066221-56066333,56066493-56066591,56066671-56066718,5606
6811-56066921,56067020-56067110
AGTACATGAAAGGTTACGCAATGCTGAAACTCGTGAAGGGGCCTCTTTTACTTCCCTTTTCGGT
CCTGCTCTTTGTCTTGGCATCATATCTAGAAGGAGAGTGCATTATGAAGCTATCAAATATGAGA
AAGAGCGAAATGCTGGATTCTTATCTCCATTTGGATATTCTGCAGCCAGTGTTGCTGCAGCCGC
TGATGCTGTGTGCTCCATGGAGTGGTTTTGGCTTTTGGCTCTAAAAAGTCAAGTAAGTAATGAA
GGACTGTACTCTATACGGATTTGGAGGTGGCGTGGTCATCTAATTCAGTATACAGTAGTTGGGC
TCGAAGGTCCTGCTATTCTACTTGTGCATGGTTTTGGAGCCTTTTTGGAGCACTACCGTGACAA
TATAAATGCCATAGCTAAACATGGGAACCATGTTTGGGGCATCACAGTGTTGGGATTTGGCCAA
TCAGAAAAGCCAAACGTTGTGTATACTGAACTCCTATGGGCTGAAATGCTGAGAGATTTTATCA
TTGAAGTTGTGGGCGAGCCTGTGCATCTAGTGGGAAACTCAATTGGTGGTTATTTTGTCTCTAT
TGTTGCTAGCCTCTGGCCTTCTTTGGTCAAGTCTGTCATCCTTATGAACACTGCTGGCAATGTC
ATTCCTGAATTTTCTTCCCGCCGGTTTCCCAGTGAGAGGCAAACCTCAGCTGCTGCAAGGCTGG
GTGCTCAACTCCTTTTATTCTATTTGAGATTGAATATCAGGAACACACTTAAAAATTTCTACCC
CACTAAAACCAACCGAGCTGATGATTGGCTTATTAATGAAATGCTGAGGGCATCCTATGATCCT
GGTGTGACAGTGGTGCTGGAAAGTATTTTCAGCTTTGACCTCTCAATCCCTTTGAATTATCTCT
TGGAAGGATACGAGGAAAAGGTTCTAATTCTGCAGGGAATGAAAGATCCACTCTTAGACTCAAA
GGCCAAACTAGCCATGCTCAAAGAACATTGTACCGGTCTTGCAATAAGGGAATTGGACGCTG
						
					

						Genomic sequence
						
							AGTACATGAAAGGTTACGCAATGCTGAAACTCGTGAAGGGGCCTCTTTTACTTCCCTTTTCGGT
CCTGCTCTTTGTCTTGGCATCATATCTAGAAGGAGAGTGCATTATGAAGCTATCAAATATGAGA
AAGAGCGAAATGCTGGATTCTTATCTCCATTTGGATATTCTGCAGCCAGTGTTGCTGCAGCCGC
TGATGCTGTGTGCTCCATGGAGGTAATTGCTGCTTTATATTGGTATTTACCTGAATATAAATGC
TCAGCTAATTATTCTTCTGAATGGTTTTGTTCTTTGTGATATCCATTGAAATCTAATGGTAGTG
CAAGGCCTTAATAAAGCATTGTAGCTGACTTTGGGTGCAGTGGTTTTGGCTTTTGGCTCTAAAA
AGTCAAGTAAGTAATGAAGGACTGTACTCTATACGGATTTGGAGGTGGCGTGGTCATCTAATTC
AGGTTTGTCATCAACAACTGATTCATACTTTTGACATTTAAATCTGTAATGTAGTTTGCAGGAT
AAGGGACTTTATGCAACCTTGATGTACTCGAGTACTTTATATGTCTTGGTTATAGATTTCTGTA
AATATTCCAGTATACAGTAGTTGGGCTCGAAGGTCCTGCTATTCTACTTGTGCATGGTTTTGGA
GCCTTTTTGGAGCACTACCGTGACAATATAAATGCCATAGCTAAACATGGGAACCATGTTTGGG
GCATCACAGTGTTGGGATTTGGCCAATCAGAAAAGCCAAACGTTGTGTATACTGAACTCCTATG
GGCTGAAATGCTGAGAGATTTTATCATTGAAGTTGTGGGCGAGCCTGTGCATCTAGTGGGAAAC
TCAATTGGTGGTATGTTATCAATCCTAACCTATTTTTTTACCTTTCCTAAGGTGACTACTAGAA
TTTCTTACTCTCTAGCTGAAAATAGCTTATCTGTTTGACTCAACAATTTGTCATTCTTTTCTTT
ACCCATCCACTTATTCTATCTCCAGGTTATTTTGTCTCTATTGTTGCTAGCCTCTGGCCTTCTT
TGGTCAAGTCTGTCATCCTTATGAACACTGCTGGCAATGTCATTCCTGAATTTTCTTCCCGCCG
GTTTCCCAGTGTAAGTTTAGCCATATCATTTGGCAATTTATGTTTGTCTATCATGTTTATTCCC
GCTCTTGTGCTGTTCGGTAAAAACTGAAAATGGAGGTTAAAATACAAGTTTTGTGGTTTTGAGT
AATAGCCGCAGCATGTGATATTCAGTATATAAATGTTTCAGGAGAGGCAAACCTCAGCTGCTGC
AAGGCTGGGTGCTCAACTCCTTTTATTCTATTTGAGATTGAATATCAGGAACACACTTAAAAAT
TTCTACCCCACTGTAAGGATCTTTCCATTGCTGCTTCCCGTATCATTTTGCACGTTGCTTGACT
GATTTCAATGGCCTTTGACTTTTGCAGAAAACCAACCGAGCTGATGATTGGCTTATTAATGAAA
TGCTGAGGGCAGTATCCTCACTGTTATTTGAATTTCAAGACCATGTACACACACTATTATAATA
TCTTCTCCCTCTGAAAAAATGAACTTAACAAACTTTAAGTCCTATGATCCTGGTGTGACAGTGG
TGCTGGAAAGTATTTTCAGCTTTGACCTCTCAATCCCTTTGAATTATCTCTTGGAAGGATACGA
GGAAAAGGTTCTAATTCTGCAGGTAAATTCCCTCTATTAAGTAAAAAAGAAGTTTATAATCTCT
CTCTACGGGTTTGTGGTCTTTTACTTACTGGTGTTTCAATCCGCATATCTTGTTAGGGAATGAA
AGATCCACTCTTAGACTCAAAGGCCAAACTAGCCATGCTCAAAGAACATTGTACCGGTCTTGCA
ATAAGGGAATTGGACGCTG
						
					

			
		

Sequences
Splice junction sequence	TCTTGTTAGGGAATGAAAGATCCACTCTTAGACTCAAAGGCCAAACTAGCCATGCTCAAAGAAC ATTGTACCGGTCTTGCAATAAGGGAATTGGACGCTGagtacatgaaaggttacgcaatgctgaa actcgtgaaggggcctcttttacttcccttttcggtcctgctctttgtcttggcatcatatcta gaaggaga
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 1022 Transcript exons: 56065236-56065449,56065596-56065685,56065822-56066077,56 066221-56066333,56066493-56066591,56066671-56066718,5606 6811-56066921,56067020-56067110 AGTACATGAAAGGTTACGCAATGCTGAAACTCGTGAAGGGGCCTCTTTTACTTCCCTTTTCGGT CCTGCTCTTTGTCTTGGCATCATATCTAGAAGGAGAGTGCATTATGAAGCTATCAAATATGAGA AAGAGCGAAATGCTGGATTCTTATCTCCATTTGGATATTCTGCAGCCAGTGTTGCTGCAGCCGC TGATGCTGTGTGCTCCATGGAGTGGTTTTGGCTTTTGGCTCTAAAAAGTCAAGTAAGTAATGAA GGACTGTACTCTATACGGATTTGGAGGTGGCGTGGTCATCTAATTCAGTATACAGTAGTTGGGC TCGAAGGTCCTGCTATTCTACTTGTGCATGGTTTTGGAGCCTTTTTGGAGCACTACCGTGACAA TATAAATGCCATAGCTAAACATGGGAACCATGTTTGGGGCATCACAGTGTTGGGATTTGGCCAA TCAGAAAAGCCAAACGTTGTGTATACTGAACTCCTATGGGCTGAAATGCTGAGAGATTTTATCA TTGAAGTTGTGGGCGAGCCTGTGCATCTAGTGGGAAACTCAATTGGTGGTTATTTTGTCTCTAT TGTTGCTAGCCTCTGGCCTTCTTTGGTCAAGTCTGTCATCCTTATGAACACTGCTGGCAATGTC ATTCCTGAATTTTCTTCCCGCCGGTTTCCCAGTGAGAGGCAAACCTCAGCTGCTGCAAGGCTGG GTGCTCAACTCCTTTTATTCTATTTGAGATTGAATATCAGGAACACACTTAAAAATTTCTACCC CACTAAAACCAACCGAGCTGATGATTGGCTTATTAATGAAATGCTGAGGGCATCCTATGATCCT GGTGTGACAGTGGTGCTGGAAAGTATTTTCAGCTTTGACCTCTCAATCCCTTTGAATTATCTCT TGGAAGGATACGAGGAAAAGGTTCTAATTCTGCAGGGAATGAAAGATCCACTCTTAGACTCAAA GGCCAAACTAGCCATGCTCAAAGAACATTGTACCGGTCTTGCAATAAGGGAATTGGACGCTG
Genomic sequence	AGTACATGAAAGGTTACGCAATGCTGAAACTCGTGAAGGGGCCTCTTTTACTTCCCTTTTCGGT CCTGCTCTTTGTCTTGGCATCATATCTAGAAGGAGAGTGCATTATGAAGCTATCAAATATGAGA AAGAGCGAAATGCTGGATTCTTATCTCCATTTGGATATTCTGCAGCCAGTGTTGCTGCAGCCGC TGATGCTGTGTGCTCCATGGAGGTAATTGCTGCTTTATATTGGTATTTACCTGAATATAAATGC TCAGCTAATTATTCTTCTGAATGGTTTTGTTCTTTGTGATATCCATTGAAATCTAATGGTAGTG CAAGGCCTTAATAAAGCATTGTAGCTGACTTTGGGTGCAGTGGTTTTGGCTTTTGGCTCTAAAA AGTCAAGTAAGTAATGAAGGACTGTACTCTATACGGATTTGGAGGTGGCGTGGTCATCTAATTC AGGTTTGTCATCAACAACTGATTCATACTTTTGACATTTAAATCTGTAATGTAGTTTGCAGGAT AAGGGACTTTATGCAACCTTGATGTACTCGAGTACTTTATATGTCTTGGTTATAGATTTCTGTA AATATTCCAGTATACAGTAGTTGGGCTCGAAGGTCCTGCTATTCTACTTGTGCATGGTTTTGGA GCCTTTTTGGAGCACTACCGTGACAATATAAATGCCATAGCTAAACATGGGAACCATGTTTGGG GCATCACAGTGTTGGGATTTGGCCAATCAGAAAAGCCAAACGTTGTGTATACTGAACTCCTATG GGCTGAAATGCTGAGAGATTTTATCATTGAAGTTGTGGGCGAGCCTGTGCATCTAGTGGGAAAC TCAATTGGTGGTATGTTATCAATCCTAACCTATTTTTTTACCTTTCCTAAGGTGACTACTAGAA TTTCTTACTCTCTAGCTGAAAATAGCTTATCTGTTTGACTCAACAATTTGTCATTCTTTTCTTT ACCCATCCACTTATTCTATCTCCAGGTTATTTTGTCTCTATTGTTGCTAGCCTCTGGCCTTCTT TGGTCAAGTCTGTCATCCTTATGAACACTGCTGGCAATGTCATTCCTGAATTTTCTTCCCGCCG GTTTCCCAGTGTAAGTTTAGCCATATCATTTGGCAATTTATGTTTGTCTATCATGTTTATTCCC GCTCTTGTGCTGTTCGGTAAAAACTGAAAATGGAGGTTAAAATACAAGTTTTGTGGTTTTGAGT AATAGCCGCAGCATGTGATATTCAGTATATAAATGTTTCAGGAGAGGCAAACCTCAGCTGCTGC AAGGCTGGGTGCTCAACTCCTTTTATTCTATTTGAGATTGAATATCAGGAACACACTTAAAAAT TTCTACCCCACTGTAAGGATCTTTCCATTGCTGCTTCCCGTATCATTTTGCACGTTGCTTGACT GATTTCAATGGCCTTTGACTTTTGCAGAAAACCAACCGAGCTGATGATTGGCTTATTAATGAAA TGCTGAGGGCAGTATCCTCACTGTTATTTGAATTTCAAGACCATGTACACACACTATTATAATA TCTTCTCCCTCTGAAAAAATGAACTTAACAAACTTTAAGTCCTATGATCCTGGTGTGACAGTGG TGCTGGAAAGTATTTTCAGCTTTGACCTCTCAATCCCTTTGAATTATCTCTTGGAAGGATACGA GGAAAAGGTTCTAATTCTGCAGGTAAATTCCCTCTATTAAGTAAAAAAGAAGTTTATAATCTCT CTCTACGGGTTTGTGGTCTTTTACTTACTGGTGTTTCAATCCGCATATCTTGTTAGGGAATGAA AGATCCACTCTTAGACTCAAAGGCCAAACTAGCCATGCTCAAAGAACATTGTACCGGTCTTGCA ATAAGGGAATTGGACGCTG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.444564284

Conservation Information
Conserved circRNAs	NA
PMCS	0.444564284

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							56065444-56065614(+)
56065243-56066881(-)
56065323-56067068(-)
						
						Potential amino acid sequence
						
							MEWFWLLALKSQVSNEGLYSIRIWRWRGHLIQYTVVGLEGPAILLVHGFGAFLEHYRDNINAIA
KHGNHVWGITVLGFGQSEKPNVVYTELLWAEMLRDFIIEVVGEPVHLVGNSIGGYFVSIVASLW
PSLVKSVILMNTAGNVIPEFSSRRFPSERQTSAAARLGAQLLLFYLRLNIRNTLKNFYPTKTNR
ADDWLINEMLRASYDPGVTVVLESIFSFDLSIPLNYLLEGYEEKVLILQGMKDPLLDSKAKLAM
LKEHCTGLAIRELDAEYMKGYAMLKLVKGPLLLPFSVLLFVLASYLEGECIMKLSNMRKSEMLD
SYLHLDILQPVLLQPLMLCAPWSGFGFWL*(+)
MYSASNSLIARPVQCSLSMASLAFESKSGSFIPCRIRTFSSYPSKR*(-)
MMPRQRAGPKREVKEAPSRVSALRNLSCTQRPIPLLQDRYNVL*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	56065444-56065614(+) 56065243-56066881(-) 56065323-56067068(-)
Potential amino acid sequence	MEWFWLLALKSQVSNEGLYSIRIWRWRGHLIQYTVVGLEGPAILLVHGFGAFLEHYRDNINAIA KHGNHVWGITVLGFGQSEKPNVVYTELLWAEMLRDFIIEVVGEPVHLVGNSIGGYFVSIVASLW PSLVKSVILMNTAGNVIPEFSSRRFPSERQTSAAARLGAQLLLFYLRLNIRNTLKNFYPTKTNR ADDWLINEMLRASYDPGVTVVLESIFSFDLSIPLNYLLEGYEEKVLILQGMKDPLLDSKAKLAM LKEHCTGLAIRELDAEYMKGYAMLKLVKGPLLLPFSVLLFVLASYLEGECIMKLSNMRKSEMLD SYLHLDILQPVLLQPLMLCAPWSGFGFWL(+) MYSASNSLIARPVQCSLSMASLAFESKSGSFIPCRIRTFSSYPSKR(-) MMPRQRAGPKREVKEAPSRVSALRNLSCTQRPIPLLQDRYNVL*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.008G284900_circ_g.1

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