Plant Circular RNA Database

Detail information of Os06g0102300_circ_g.1

			
						 General Information
					
						CircRNA Name
						Os06g0102300_circ_g.1
					
						ID in PlantcircBase
						osa_circ_029276
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr6: 172105-173070  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os06g0102300						
					
						Parent gene annotation
						
							Similar to Phytochelatin synthase. (Os06t0102300-01);Similar to 
Glutathione gamma-glutamylcysteinyltransferase 1. (Os06t0102300-
02);Phytochelatin synthase 2, Cadmium (Cd) and arsenic (As) tole
rance (Os06t0102300-03);Splicing variant of phytochelatin syntha
se 2 (Os06t0102300-04)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os06t0102300-03:3
Os06t0102300-02:3
Os06t0102300-01:3

General Information
CircRNA Name	Os06g0102300_circ_g.1
ID in PlantcircBase	osa_circ_029276
Alias	NA
Organism	Oryza sativa
Position	chr6: 172105-173070 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ue-circRNA
Identification method	CIRCexplorer
Parent gene	Os06g0102300
Parent gene annotation	Similar to Phytochelatin synthase. (Os06t0102300-01);Similar to Glutathione gamma-glutamylcysteinyltransferase 1. (Os06t0102300- 02);Phytochelatin synthase 2, Cadmium (Cd) and arsenic (As) tole rance (Os06t0102300-03);Splicing variant of phytochelatin syntha se 2 (Os06t0102300-04)
Parent gene strand	+
Alternative splicing	NA
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os06t0102300-03:3 Os06t0102300-02:3 Os06t0102300-01:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ACATCCTAGCACATGGGTGGGCATTAAAGACGAGAGGCTGAAAGCTGAATTCCAGAGTCTTATT
TCAACAGACATTCTTCATGATGATCTTAAACGAGAGgttcatgcttatctcaaggcacactgca
gctccttcattgctctacacagtggtaaacatgccaatgcttggtatatcgaattcaagtgggt
acattgat

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GTTCATGCTTATCTCAAGGCACACTGCAGCTCCTTCATTGCTCTACACAGTGGTAAACATGCCA
ATGCTTGGTATATCGAATTCAAGTGGGTACATTGATATAATTTCCTTTATATCATTCCTTTAGC
TTTTGTGCAGAGTTGCAGAGATGAAAGCTGGAAAAGCATGGCGAAGTATTGCATGGAAGATGTA
CCCGATCTTCTTAAGGATGAGAGTGTAGACAATGTTCCAGCACTTCTGTCCCGCTTAGTGAAAT
CCCTTCCTGCCAATGCTGGAAATTTGATCAAATGGGTTATTGAAGTTAGGAGACAAGAGGAAGG
AGGATCAGGATTAAGCAAAGAGGAGGAAGAAAGGCTTATTTTGAAGGTCATCTTCAACCTTTTC
TTTTCAATCTGGGATTCTGGATCAGAATTTTCTTTTTGTCTCTTTTAACACACCTTACACTGTG
TGCACTTGCAGGAAATGATACTACAGCAAGTCCGTGATACTGAGCTTTTTAGATTAGTCCGTGA
ACTGCAATTCACTAAGCAGCCATGTTGTAGTTGCTCATATTCAAGTGATGATGATTCCTTTACC
CGGATTGCAGCCTCTGTGTGCTGTCAAGGGGCCGCATTGCTAACAGGGAATCTTTCATCAAAAG
ATGGGTTCTGCTGCAGAGAAACTTGCTTCAAATGTGTACAAGTGGATGGTGATGGGCCTAAGAC
TGTCGTTACAGGCACAGCGGTTTCAGGAGTCAATGAACAAAGTGTTGATATGCTTCTACCGATA
TCCACATTGGAAACAAGCGTGTGCAATTCAAATTCAAGCAACGAGGTTGTCAAATATCCATCTA
GAACAGATATTTTAACTGTTCTATTGCTGGCTTTACATCCTAGCACATGGGTGGGCATTAAAGA
CGAGAGGCTGAAAGCTGAATTCCAGAGTCTTATTTCAACAGACATTCTTCATGATGATCTTAAA
CGAGAG

Sequences
Splice junction sequence	ACATCCTAGCACATGGGTGGGCATTAAAGACGAGAGGCTGAAAGCTGAATTCCAGAGTCTTATT TCAACAGACATTCTTCATGATGATCTTAAACGAGAGgttcatgcttatctcaaggcacactgca gctccttcattgctctacacagtggtaaacatgccaatgcttggtatatcgaattcaagtgggt acattgat
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GTTCATGCTTATCTCAAGGCACACTGCAGCTCCTTCATTGCTCTACACAGTGGTAAACATGCCA ATGCTTGGTATATCGAATTCAAGTGGGTACATTGATATAATTTCCTTTATATCATTCCTTTAGC TTTTGTGCAGAGTTGCAGAGATGAAAGCTGGAAAAGCATGGCGAAGTATTGCATGGAAGATGTA CCCGATCTTCTTAAGGATGAGAGTGTAGACAATGTTCCAGCACTTCTGTCCCGCTTAGTGAAAT CCCTTCCTGCCAATGCTGGAAATTTGATCAAATGGGTTATTGAAGTTAGGAGACAAGAGGAAGG AGGATCAGGATTAAGCAAAGAGGAGGAAGAAAGGCTTATTTTGAAGGTCATCTTCAACCTTTTC TTTTCAATCTGGGATTCTGGATCAGAATTTTCTTTTTGTCTCTTTTAACACACCTTACACTGTG TGCACTTGCAGGAAATGATACTACAGCAAGTCCGTGATACTGAGCTTTTTAGATTAGTCCGTGA ACTGCAATTCACTAAGCAGCCATGTTGTAGTTGCTCATATTCAAGTGATGATGATTCCTTTACC CGGATTGCAGCCTCTGTGTGCTGTCAAGGGGCCGCATTGCTAACAGGGAATCTTTCATCAAAAG ATGGGTTCTGCTGCAGAGAAACTTGCTTCAAATGTGTACAAGTGGATGGTGATGGGCCTAAGAC TGTCGTTACAGGCACAGCGGTTTCAGGAGTCAATGAACAAAGTGTTGATATGCTTCTACCGATA TCCACATTGGAAACAAGCGTGTGCAATTCAAATTCAAGCAACGAGGTTGTCAAATATCCATCTA GAACAGATATTTTAACTGTTCTATTGCTGGCTTTACATCCTAGCACATGGGTGGGCATTAAAGA CGAGAGGCTGAAAGCTGAATTCCAGAGTCTTATTTCAACAGACATTCTTCATGATGATCTTAAA CGAGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_016262 						
					
						PMCS
						0.20758511

Conservation Information
Conserved circRNAs	osi_circ_016262
PMCS	0.20758511

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							172271-173067(+)
173052-172321(+)
						
						Potential amino acid sequence
						
							MAKYCMEDVPDLLKDESVDNVPALLSRLVKSLPANAGNLIKWVIEVRRQEEGGSGLSKEEEERL
ILKEMILQQVRDTELFRLVRELQFTKQPCCSCSYSSDDDSFTRIAASVCCQGAALLTGNLSSKD
GFCCRETCFKCVQVDGDGPKTVVTGTAVSGVNEQSVDMLLPISTLETSVCNSNSSNEVVKYPSR
TDILTVLLLALHPSTWVGIKDERLKAEFQSLISTDILHDDLKREVHAYLKAHCSSFIALHSAFV
QSCRDESWKSMAKYCMEDVPDLLKDESVDNVPALLSRLVKSLPANAGNLIKWVIEVRRQEEGGS
GLSKEEEERLILKEMILQQVRDTELFRLVRELQFTKQPCCSCSYSSDDDSFTRIAASVCCQGAA
LLTGNLSSKDGFCCRETCFKCVQVDGDGPKTVVTGTAVSGVNEQSVDMLLPISTLETSVCNSNS
SNEVVKYPSRTDILTVLLLALHPSTWVGIKDERLKAEFQSLISTDILHDDLKREVHAYLKAHCS
SFIALHSAFVQSCRDESWKSMAKYCMEDVPDLLKDESVDNVPALLSRLVKSLPANAGNLIKWVI
EVRRQEEGGSGLSKEEEERLILKEMILQQVRDTELFRLVRELQFTKQPCCSCSYSSDDDSFTRI
AASVCCQGAALLTGNLSSKDGFCCRETCFKCVQVDGDGPKTVVTGTAVSGVNEQSVDMLLPIST
LETSVCNSNSSNEVVKYPSRTDILTVLLLALHPSTWVGIKDERLKAEFQSLISTDILHDDLKRE
VHAYLKAHCSSFIALHSAFVQSCRDESWKSMAKYCMEDVPDLLKDESVDNVPALLSRLVKSLPA
NAGNLIKWVIEVRRQEEGGSGLSKEEEERLILKEMILQQVRDTELFRLVRELQFTKQPCCSCSY
SSDDDSFTRIAASVCCQGAALLTGNLSSKDGFCCRETCFKCVQVDGDGPKTVVTGTAVSGVNEQ
SVDMLLPISTLETSVCNSNSSNEVVKYPSRTDILTVLLLALHPSTWVGIKDERLKAEFQSLIST
DILHDDLKRE(+)
MMILNERFMLISRHTAAPSLLYTVLLCRVAEMKAGKAWRSIAWKMYPIFLRMRV*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	172271-173067(+) 173052-172321(+)
Potential amino acid sequence	MAKYCMEDVPDLLKDESVDNVPALLSRLVKSLPANAGNLIKWVIEVRRQEEGGSGLSKEEEERL ILKEMILQQVRDTELFRLVRELQFTKQPCCSCSYSSDDDSFTRIAASVCCQGAALLTGNLSSKD GFCCRETCFKCVQVDGDGPKTVVTGTAVSGVNEQSVDMLLPISTLETSVCNSNSSNEVVKYPSR TDILTVLLLALHPSTWVGIKDERLKAEFQSLISTDILHDDLKREVHAYLKAHCSSFIALHSAFV QSCRDESWKSMAKYCMEDVPDLLKDESVDNVPALLSRLVKSLPANAGNLIKWVIEVRRQEEGGS GLSKEEEERLILKEMILQQVRDTELFRLVRELQFTKQPCCSCSYSSDDDSFTRIAASVCCQGAA LLTGNLSSKDGFCCRETCFKCVQVDGDGPKTVVTGTAVSGVNEQSVDMLLPISTLETSVCNSNS SNEVVKYPSRTDILTVLLLALHPSTWVGIKDERLKAEFQSLISTDILHDDLKREVHAYLKAHCS SFIALHSAFVQSCRDESWKSMAKYCMEDVPDLLKDESVDNVPALLSRLVKSLPANAGNLIKWVI EVRRQEEGGSGLSKEEEERLILKEMILQQVRDTELFRLVRELQFTKQPCCSCSYSSDDDSFTRI AASVCCQGAALLTGNLSSKDGFCCRETCFKCVQVDGDGPKTVVTGTAVSGVNEQSVDMLLPIST LETSVCNSNSSNEVVKYPSRTDILTVLLLALHPSTWVGIKDERLKAEFQSLISTDILHDDLKRE VHAYLKAHCSSFIALHSAFVQSCRDESWKSMAKYCMEDVPDLLKDESVDNVPALLSRLVKSLPA NAGNLIKWVIEVRRQEEGGSGLSKEEEERLILKEMILQQVRDTELFRLVRELQFTKQPCCSCSY SSDDDSFTRIAASVCCQGAALLTGNLSSKDGFCCRETCFKCVQVDGDGPKTVVTGTAVSGVNEQ SVDMLLPISTLETSVCNSNSSNEVVKYPSRTDILTVLLLALHPSTWVGIKDERLKAEFQSLIST DILHDDLKRE(+) MMILNERFMLISRHTAAPSLLYTVLLCRVAEMKAGKAWRSIAWKMYPIFLRMRV*(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os06g0102300_circ_g.1

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