Plant Circular RNA Database

Detail information of Os04g0379300_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os04g0379300_circ_g.2
					
						ID in PlantcircBase
						osa_circ_023564
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr4: 18524429-18524823  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os04g0379300						
					
						Parent gene annotation
						
							Methyltransferase type 11 domain containing protein. (Os04t03793
00-01);Similar to predicted protein. (Os04t0379300-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os04t0379300-01:3
Os04t0379300-02:3

General Information
CircRNA Name	Os04g0379300_circ_g.2
ID in PlantcircBase	osa_circ_023564
Alias	NA
Organism	Oryza sativa
Position	chr4: 18524429-18524823 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os04g0379300
Parent gene annotation	Methyltransferase type 11 domain containing protein. (Os04t03793 00-01);Similar to predicted protein. (Os04t0379300-02)
Parent gene strand	+
Alternative splicing	NA
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os04t0379300-01:3 Os04t0379300-02:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCCTAATGTTATTGTTTTGCTTCTTTTTTTTTCAGAATGATTTATCTCCCCTTGTTAAGGATCT
GGAACCTGGAAATCCTGAAGAGGAAGCTCGTATACCgttcgagcacatgaatggctttttgctt
cagaagaaggacagtggcatgttgttgaaagtgcaaaggctgctcgtctaattatggtacaact
gtgatgga

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GTTCGAGCACATGAATGGCTTTTTGCTTCAGAAGAAGGACAGTGGCATGTTGTTGAAAGTGCAA
AGGCTGCTCGTCTAATTATGGTACAACTGTGATGGATGCAAGCCTAGTGTGATTATAATTTGAT
ATTTGGGATCACTTTACAGCATTTTCATTGCTTATATTTGTGCCATGAACTTACCTTTACCTTA
GGTATTCTTGGATAGCAGACATGCAAATGCTGACATGGATGTTATCAAGGTGATTGTTTGCAAA
TTAGGATGTTGTGCAAATTCCACAGGCTAATACACACTATCCTAATGTTATTGTTTTGCTTCTT
TTTTTTTCAGAATGATTTATCTCCCCTTGTTAAGGATCTGGAACCTGGAAATCCTGAAGAGGAA
GCTCGTATACC

Sequences
Splice junction sequence	TCCTAATGTTATTGTTTTGCTTCTTTTTTTTTCAGAATGATTTATCTCCCCTTGTTAAGGATCT GGAACCTGGAAATCCTGAAGAGGAAGCTCGTATACCgttcgagcacatgaatggctttttgctt cagaagaaggacagtggcatgttgttgaaagtgcaaaggctgctcgtctaattatggtacaact gtgatgga
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GTTCGAGCACATGAATGGCTTTTTGCTTCAGAAGAAGGACAGTGGCATGTTGTTGAAAGTGCAA AGGCTGCTCGTCTAATTATGGTACAACTGTGATGGATGCAAGCCTAGTGTGATTATAATTTGAT ATTTGGGATCACTTTACAGCATTTTCATTGCTTATATTTGTGCCATGAACTTACCTTTACCTTA GGTATTCTTGGATAGCAGACATGCAAATGCTGACATGGATGTTATCAAGGTGATTGTTTGCAAA TTAGGATGTTGTGCAAATTCCACAGGCTAATACACACTATCCTAATGTTATTGTTTTGCTTCTT TTTTTTTCAGAATGATTTATCTCCCCTTGTTAAGGATCTGGAACCTGGAAATCCTGAAGAGGAA GCTCGTATACC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.236965042

Conservation Information
Conserved circRNAs	NA
PMCS	0.236965042

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							18524511-18524504(+)
18524642-18524439(+)
18524658-18524780(-)
18524442-18524780(-)
						
						Potential amino acid sequence
						
							MVFLDSRHANADMDVIKNDLSPLVKDLEPGNPEEEARIPFEHMNGFLLQKKDSGMLLKVQRLLV
*(+)
MQMLTWMLSRMIYLPLLRIWNLEILKRKLVYRSST*(+)
MSAFACLLSKNTIIRRAAFALSTTCHCPSSEAKSHSCARTVYELPLQDFQVPDP*(-)
MCSNGIRASSSGFPGSRSLTRGDKSFLITSMSAFACLLSKNTIIRRAAFALSTTCHCPSSEAKS
HSCARTVYELPLQDFQVPDP*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	18524511-18524504(+) 18524642-18524439(+) 18524658-18524780(-) 18524442-18524780(-)
Potential amino acid sequence	MVFLDSRHANADMDVIKNDLSPLVKDLEPGNPEEEARIPFEHMNGFLLQKKDSGMLLKVQRLLV (+) MQMLTWMLSRMIYLPLLRIWNLEILKRKLVYRSST(+) MSAFACLLSKNTIIRRAAFALSTTCHCPSSEAKSHSCARTVYELPLQDFQVPDP(-) MCSNGIRASSSGFPGSRSLTRGDKSFLITSMSAFACLLSKNTIIRRAAFALSTTCHCPSSEAKS HSCARTVYELPLQDFQVPDP(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os04g0379300_circ_g.2

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