Plant Circular RNA Database

Detail information of Os05g0159000_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os05g0159000_circ_g.3
					
						ID in PlantcircBase
						osa_circ_026715
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr5: 3479200-3484250  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os05g0159000						
					
						Parent gene annotation
						
							HECT domain containing protein. (Os05t0159000-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os05g0159000_circ_g.2 Os05g0159000_circ_g.4 Os05g0159000_circ_g.5 Os05g0159000_circ_g.6 Os05g0159000_circ_g.7 						
					
						Support reads
						2
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os05t0159000-01:9

General Information
CircRNA Name	Os05g0159000_circ_g.3
ID in PlantcircBase	osa_circ_026715
Alias	NA
Organism	Oryza sativa
Position	chr5: 3479200-3484250 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os05g0159000
Parent gene annotation	HECT domain containing protein. (Os05t0159000-01)
Parent gene strand	+
Alternative splicing	Os05g0159000_circ_g.2 Os05g0159000_circ_g.4 Os05g0159000_circ_g.5 Os05g0159000_circ_g.6 Os05g0159000_circ_g.7
Support reads	2
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os05t0159000-01:9

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							ATGTGGTTGATACTTGATAAATGCACAGGTTCGTGACCGGATGTTCTCGTGGACCACTCCTTGG
ATTTCAGTACCTCGAACCAAAATTCTGCATTCAGAGattcgagtggtgtttgttaatgagcatg
gtgttgaagaggctgggattgacggaggtggaattttcaaggatttcatggaaaatatcactcg
agccgctt

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	ATGTGGTTGATACTTGATAAATGCACAGGTTCGTGACCGGATGTTCTCGTGGACCACTCCTTGG ATTTCAGTACCTCGAACCAAAATTCTGCATTCAGAGattcgagtggtgtttgttaatgagcatg gtgttgaagaggctgggattgacggaggtggaattttcaaggatttcatggaaaatatcactcg agccgctt
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.094625949

Conservation Information
Conserved circRNAs	NA
PMCS	0.094625949

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							3479276-3480133(+)
3484192-3480133(+)
3479278-3483864(-)
						
						Potential amino acid sequence
						
							MENITRAAFDVQYGLFKETADHLLYPNPGSGLVHEQHLQYFHFLGSLLGKAIYEGILVDLPFAT
FFLSKLKHKYNFLNDLPSLDPELYRHLLSLKHYDGDISQLELYFVIVNNEYGEQSEEELLPGGR
DMRVTSENVITYIHLVANHRLNYQIRAQSTHFLRGFQQLIPNEWINMFNEHEFQVLISGSLESL
DIDDLRSNTNYSGGYHPDHELIDIFWEVLKSFSSHNQKKFLKFVTGCSRGPLLGFQYLEPKFCI
QRFEWCLLMSMVLKRLGLTEVEFSRISWKISLEPLLMCSTVYSRRQPIIFSTQILDLDWSMNSI
CNTSIFLAVSLGRQSMRAYLLICHLQHSS*(+)
MFSWTTPWISVPRTKILHSEIRVVFVNEHGVEEAGIDGGGIFKDFMENITRAAFDVQYGLFKET
ADHLLYPNPGSGLVHEQHLQYFHFLGSLLGKAIYEGILVDLPFATFFLSKLKHKYNFLNDLPSL
DPELYRHLLSLKHYDGDISQLELYFVIVNNEYGEQSEEELLPGGRDMRVTSENVITYIHLVANH
RLNYQIRAQSTHFLRGFQQLIPNEWINMFNEHEFQVLISGSLESLDIDDLRSNTNYSGGYHPDH
ELIDIFWEVLKSFSSHNQKKFLKFVTGCSRGPLLGFQYLEPKFCIQRFEWCLLMSMVLKRLGLT
EVEFSRISWKISLEPLLMCSTVYSRRQPIIFSTQILDLDWSMNSICNTSIFLAVSLGRQSMRAY
LLICHLQHSS*(+)
MKSLKIPPPSIPASSTPCSLTNTTRISECRILVRGTEIQGVVHENIRSRT*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	3479276-3480133(+) 3484192-3480133(+) 3479278-3483864(-)
Potential amino acid sequence	MENITRAAFDVQYGLFKETADHLLYPNPGSGLVHEQHLQYFHFLGSLLGKAIYEGILVDLPFAT FFLSKLKHKYNFLNDLPSLDPELYRHLLSLKHYDGDISQLELYFVIVNNEYGEQSEEELLPGGR DMRVTSENVITYIHLVANHRLNYQIRAQSTHFLRGFQQLIPNEWINMFNEHEFQVLISGSLESL DIDDLRSNTNYSGGYHPDHELIDIFWEVLKSFSSHNQKKFLKFVTGCSRGPLLGFQYLEPKFCI QRFEWCLLMSMVLKRLGLTEVEFSRISWKISLEPLLMCSTVYSRRQPIIFSTQILDLDWSMNSI CNTSIFLAVSLGRQSMRAYLLICHLQHSS(+) MFSWTTPWISVPRTKILHSEIRVVFVNEHGVEEAGIDGGGIFKDFMENITRAAFDVQYGLFKET ADHLLYPNPGSGLVHEQHLQYFHFLGSLLGKAIYEGILVDLPFATFFLSKLKHKYNFLNDLPSL DPELYRHLLSLKHYDGDISQLELYFVIVNNEYGEQSEEELLPGGRDMRVTSENVITYIHLVANH RLNYQIRAQSTHFLRGFQQLIPNEWINMFNEHEFQVLISGSLESLDIDDLRSNTNYSGGYHPDH ELIDIFWEVLKSFSSHNQKKFLKFVTGCSRGPLLGFQYLEPKFCIQRFEWCLLMSMVLKRLGLT EVEFSRISWKISLEPLLMCSTVYSRRQPIIFSTQILDLDWSMNSICNTSIFLAVSLGRQSMRAY LLICHLQHSS(+) MKSLKIPPPSIPASSTPCSLTNTTRISECRILVRGTEIQGVVHENIRSRT*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os05g0159000_circ_g.3

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