Plant Circular RNA Database

Detail information of POPTR_0016s02870_circ_g.1

			
						 General Information
					
						CircRNA Name
						POPTR_0016s02870_circ_g.1
					
						ID in PlantcircBase
						pop_circ_003818
					
						Alias
						Chr16:1613370-1616155
					
						Organism
						
							Populus trichocarpa						
					
						Position
						
							chr16: 1414440-1417225  JBrowse»						
					
						Reference genome
						
							Populus trichocarpa genome v3.0 						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							POPTR_0016s02870						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						NA
					
						Tissues
						stem xylem
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						POPTR_0016s02870.1:10

General Information
CircRNA Name	POPTR_0016s02870_circ_g.1
ID in PlantcircBase	pop_circ_003818
Alias	Chr16:1613370-1616155
Organism	Populus trichocarpa
Position	chr16: 1414440-1417225 JBrowse»
Reference genome	Populus trichocarpa genome v3.0
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	POPTR_0016s02870
Parent gene annotation	NA
Parent gene strand	-
Alternative splicing	NA
Support reads	NA
Tissues	stem xylem
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	POPTR_0016s02870.1:10

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TTTTTTCTGTAAAACAGGAAAAACGACCCCATTGGCTGTGATCTGATGTTTCAAAAAAGTCCCG
CAAAAAACAAAAGGCTGCATAGGGCTTTAACCATTGcgtcatgtagctgtattgagaaggaata
ccaaactccaggtcaggttcactggacatacgttgaatgcgtaaacctatcaatcctagttctt
gcatgacc

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							CGTCATGTAGCTGTATTGAGAAGGAATACCAAACTCCAGGTCAGGTTCACTGGACATACGTTGA
ATGCGTAAACCTATCAATCCTAGTTCTTGCATGACCTGCAAACATTATCCAATCATGCTTAATT
TTCATGGAATGCAGATTGGAAGAAATAAAGTAAGCGAAAAAGAGAAGAGATGTAGGCAGTTAGA
GTTGAAGTTGGGAAGGATTCGACAAACATACAGGGTGAACACAAGCAGGAATTAGACCCAGATC
TTCCCCACAGGCTAGCATATCTGATGAGTCCAGGAGAGCAGGCAAAGTCTTCAAAGCATTTTGC
CTCCATAGATTTTCTTGCCGATGGAAATAGTAATCATAGTACAGTCTTTTGAGAACATTTTTGC
TGTATTGCATAACAAGTAAGAGAAGCTATATCAGCAGGAATTGAGAGGTTTGATGAGATGCAAG
GAAACAAACTAGTACTTGGTTATAGAACACCAGAATTATTGGTAAGCAAAAAGGGGATGAAAAG
CTTTAGTTTACTACATTCTTCTATGGGTCACTCAATCCAAATGAACTAGGAATTTCTCACAGTA
GATATGATTAGGGTACCTGTGGTCATCCAAATCCTGAAAACTTGAAGTGTCCTCAAGATTGAAA
CGAGGGTAGAATTTACTTTCATCCTCAGGATCTCTAATAAGAACTATATTCTGCAAACAGAATT
AATAGATAAAAACCAAGAACCGAGACTAGTAATGAGAACAACCATCATCAGTCACTCTAAAGCA
GTCAAGAGACAGGTCTAACATAAATTCCATATAACAAACTTGTGGTAATATAAATATTTTTCCA
CTATATGTTACCTTCAGAAGATCAAACAGGTCACGGCGTATCTTGTCCTCGCTCTCCAATAACA
TGGACTTTTCTGCAAGCATCTTCAACTTGGAAGCAATCTTTTTCTCCGTGTCACAATCCTCCTT
GAACTAGATTAATTTGAAGAAATATGAGTCAACTAATTTTTCATGCCAAAACAAATGCACCAAA
TATGATACAATATGATTGAAGCAGATATAAAAAGATATGAGATAATGAGAAGGGATAAGAATTC
AGAATCACTTTCCTAACAGTAAGACTAAAATTGAGGCGTAGGGAATATAAGTCGGAGCTTTAAC
AGAATGGAGACCATAAAACAACTTGAAAGAACCAAGAAGCGATTCAATGGAAAAAAATTAATAT
CAGAAATTGCAGACAGGTCAGTCAGTTCATGTGACAAGGTTCCTTTATGACATGAATTCACGAG
CCTATTTGAGGCTCGATGTGAGTCACAAATTCATCACTAGACCAACCTAGGTTCAGTCAAAATG
CTAAGAGGTAACCTCATATTATTGACCAGAAACATAAATTTACCACATAGCGGCCCTTCTGATA
ATCGTTCAGAAAATTTGACACAATAAAGGTCCAAGAAGCTCCAAATCTTTCCTGATATCAGGAA
AAAAAAAAAAAAAGAGAAGTGTAAATCAACACGCTCATAGTATCTGATTTAGTTTTTAGGTCAT
ATGTCAAATGCAATGAGTTGATAACCATCAGAAGTGTGGACCATATAGCCAACACAGAAAAGTC
AGTAACCTGTACAAATTCCTGCCGGATATATGGGAGGCTCAGGCGATCAAAGTCCCAAATTCCT
TCTCTTTCAAGTTCTTCCTGCAGCAGATGCATTATTTGAACATGTTTAATAGAACATGAAAAAG
GAATTTCTCTCACAAGTGCTTCAAGTACTAGTGCTGCAAGGGTAGCTTAGGCTTTATAAGAACT
CCAGTTCCCTATACCAAATCCTAAAATTCTCTTGCATCACTTAAAACTCACTTTTCATGCAGGA
AGGCTAGGAGGGTCCTACTATACCTTACTTAGAGGGATAGATGGTCTGAATTTTCCAATCAGAC
CTGTCATGGCATGCTCTGGAAGCTCCCAGATCCTAAAGAAACCCAGAATATGATCAATCCTGTA
AGCTGTAAAGTATTTTGCCATCTGTCATAGAAACAAATAAGTTTTTCATCAGAAAGAGATGAAA
GCTCAGTTAAGACAAGATCATCGTATTCAGAGTACAAAACCTGTGTTAGACGAGCACGCCACCA
AGCATAGTTATCTTTTGACATTTCCTCCCAATTATAAGTTGGAAAGCCCCAATTCTGCCCATTT
TTATCAAAGTAATCTGGAGGTGCTCCTGTGGAGGTGTTCATGCGGAACAAATTTGGATAGACCC
AGGTATCTACGCTGTTTCTGTCTACACCAATAGGTAGATCTCCTTTCAATATGACACCTTTCTT
TCTTGCATATTCTGCAGCTTCTGTCAACTGTCAGTTCATTATGACACAAACAGTATAAGACCGA
CTGCTGGCTTAAGAGCAATTACAAATGGGAGACAAATATCATCAGACCAAATGAAAATACCTGT
GTATGCAAATGGAATTGGATATAATAATGGAAGCGAATTATGTCATGGTGCAAGCTGTCTTTTG
AGACAAGTTTCTCAACCTGCATGAAAATGGTGCCATTTTGTCAGAGAATTTAAATAACATAACT
TTGGTTATGAAATTGAAACAAAGATCTATATTACCTAGTTATCATGTATGATAAGATCCAATAA
AATTTGACGAATGATATTTAATCTTAGGTTAGATGAGTGAAAACCCAAAGAGATAACAACCTTT
TTTTCTGTAAAACAGGAAAAACGACCCCATTGGCTGTGATCTGATGTTTCAAAAAAGTCCCGCA
AAAAACAAAAGGCTGCATAGGGCTTTAACCATTG
						
					

			
		

Sequences
Splice junction sequence	TTTTTTCTGTAAAACAGGAAAAACGACCCCATTGGCTGTGATCTGATGTTTCAAAAAAGTCCCG CAAAAAACAAAAGGCTGCATAGGGCTTTAACCATTGcgtcatgtagctgtattgagaaggaata ccaaactccaggtcaggttcactggacatacgttgaatgcgtaaacctatcaatcctagttctt gcatgacc
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CGTCATGTAGCTGTATTGAGAAGGAATACCAAACTCCAGGTCAGGTTCACTGGACATACGTTGA ATGCGTAAACCTATCAATCCTAGTTCTTGCATGACCTGCAAACATTATCCAATCATGCTTAATT TTCATGGAATGCAGATTGGAAGAAATAAAGTAAGCGAAAAAGAGAAGAGATGTAGGCAGTTAGA GTTGAAGTTGGGAAGGATTCGACAAACATACAGGGTGAACACAAGCAGGAATTAGACCCAGATC TTCCCCACAGGCTAGCATATCTGATGAGTCCAGGAGAGCAGGCAAAGTCTTCAAAGCATTTTGC CTCCATAGATTTTCTTGCCGATGGAAATAGTAATCATAGTACAGTCTTTTGAGAACATTTTTGC TGTATTGCATAACAAGTAAGAGAAGCTATATCAGCAGGAATTGAGAGGTTTGATGAGATGCAAG GAAACAAACTAGTACTTGGTTATAGAACACCAGAATTATTGGTAAGCAAAAAGGGGATGAAAAG CTTTAGTTTACTACATTCTTCTATGGGTCACTCAATCCAAATGAACTAGGAATTTCTCACAGTA GATATGATTAGGGTACCTGTGGTCATCCAAATCCTGAAAACTTGAAGTGTCCTCAAGATTGAAA CGAGGGTAGAATTTACTTTCATCCTCAGGATCTCTAATAAGAACTATATTCTGCAAACAGAATT AATAGATAAAAACCAAGAACCGAGACTAGTAATGAGAACAACCATCATCAGTCACTCTAAAGCA GTCAAGAGACAGGTCTAACATAAATTCCATATAACAAACTTGTGGTAATATAAATATTTTTCCA CTATATGTTACCTTCAGAAGATCAAACAGGTCACGGCGTATCTTGTCCTCGCTCTCCAATAACA TGGACTTTTCTGCAAGCATCTTCAACTTGGAAGCAATCTTTTTCTCCGTGTCACAATCCTCCTT GAACTAGATTAATTTGAAGAAATATGAGTCAACTAATTTTTCATGCCAAAACAAATGCACCAAA TATGATACAATATGATTGAAGCAGATATAAAAAGATATGAGATAATGAGAAGGGATAAGAATTC AGAATCACTTTCCTAACAGTAAGACTAAAATTGAGGCGTAGGGAATATAAGTCGGAGCTTTAAC AGAATGGAGACCATAAAACAACTTGAAAGAACCAAGAAGCGATTCAATGGAAAAAAATTAATAT CAGAAATTGCAGACAGGTCAGTCAGTTCATGTGACAAGGTTCCTTTATGACATGAATTCACGAG CCTATTTGAGGCTCGATGTGAGTCACAAATTCATCACTAGACCAACCTAGGTTCAGTCAAAATG CTAAGAGGTAACCTCATATTATTGACCAGAAACATAAATTTACCACATAGCGGCCCTTCTGATA ATCGTTCAGAAAATTTGACACAATAAAGGTCCAAGAAGCTCCAAATCTTTCCTGATATCAGGAA AAAAAAAAAAAAAGAGAAGTGTAAATCAACACGCTCATAGTATCTGATTTAGTTTTTAGGTCAT ATGTCAAATGCAATGAGTTGATAACCATCAGAAGTGTGGACCATATAGCCAACACAGAAAAGTC AGTAACCTGTACAAATTCCTGCCGGATATATGGGAGGCTCAGGCGATCAAAGTCCCAAATTCCT TCTCTTTCAAGTTCTTCCTGCAGCAGATGCATTATTTGAACATGTTTAATAGAACATGAAAAAG GAATTTCTCTCACAAGTGCTTCAAGTACTAGTGCTGCAAGGGTAGCTTAGGCTTTATAAGAACT CCAGTTCCCTATACCAAATCCTAAAATTCTCTTGCATCACTTAAAACTCACTTTTCATGCAGGA AGGCTAGGAGGGTCCTACTATACCTTACTTAGAGGGATAGATGGTCTGAATTTTCCAATCAGAC CTGTCATGGCATGCTCTGGAAGCTCCCAGATCCTAAAGAAACCCAGAATATGATCAATCCTGTA AGCTGTAAAGTATTTTGCCATCTGTCATAGAAACAAATAAGTTTTTCATCAGAAAGAGATGAAA GCTCAGTTAAGACAAGATCATCGTATTCAGAGTACAAAACCTGTGTTAGACGAGCACGCCACCA AGCATAGTTATCTTTTGACATTTCCTCCCAATTATAAGTTGGAAAGCCCCAATTCTGCCCATTT TTATCAAAGTAATCTGGAGGTGCTCCTGTGGAGGTGTTCATGCGGAACAAATTTGGATAGACCC AGGTATCTACGCTGTTTCTGTCTACACCAATAGGTAGATCTCCTTTCAATATGACACCTTTCTT TCTTGCATATTCTGCAGCTTCTGTCAACTGTCAGTTCATTATGACACAAACAGTATAAGACCGA CTGCTGGCTTAAGAGCAATTACAAATGGGAGACAAATATCATCAGACCAAATGAAAATACCTGT GTATGCAAATGGAATTGGATATAATAATGGAAGCGAATTATGTCATGGTGCAAGCTGTCTTTTG AGACAAGTTTCTCAACCTGCATGAAAATGGTGCCATTTTGTCAGAGAATTTAAATAACATAACT TTGGTTATGAAATTGAAACAAAGATCTATATTACCTAGTTATCATGTATGATAAGATCCAATAA AATTTGACGAATGATATTTAATCTTAGGTTAGATGAGTGAAAACCCAAAGAGATAACAACCTTT TTTTCTGTAAAACAGGAAAAACGACCCCATTGGCTGTGATCTGATGTTTCAAAAAAGTCCCGCA AAAAACAAAAGGCTGCATAGGGCTTTAACCATTG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.154086962

Conservation Information
Conserved circRNAs	NA
PMCS	0.154086962

			
				
						 Functional Information
					

				
						Coding potential
						Y
					

						Potential coding position
						
							1417172-1414534(+)
1416658-1414442(-)
						
					

						Potential amino acid sequence
						
							MFQKSPAKNKRLHRALTIASCSCIEKEYQTPGQVHWTYVECVNLSILVLA*(+)
MNTSTGAPPDYFDKNGQNWGFPTYNWEEMSKDNYAWWRARLTQMAKYFTAYRIDHILGFFRIWE
LPEHAMTGLIGKFRPSIPLSKEELEREGIWDFDRLSLPYIRQEFVQERFGASWTFIVSNFLNDY
QKGRYVFKEDCDTEKKIASKLKMLAEKSMLLESEDKIRRDLFDLLKNIVLIRDPEDESKFYPRF
NLEDTSSFQDLDDHSKNVLKRLYYDYYFHRQENLWRQNALKTLPALLDSSDMLACGEDLGLIPA
CVHPVMQELGLIGLRIQRMSSEPDLEFGIPSQYSYMTQWLKPYAAFCFLRDFFETSDHSQWGRF
SCFTEKKVEKLVSKDSLHHDIIRFHYYIQFHLHTQLTEAAEYARKKGVILKGDLPIGVDRNSVD
TWVYPNLFRMNTSTGAPPDYFDKNGQNWGFPTYNWEEMSKDNYAWWRARLTQMAKYFTAYRIDH
ILGFFRIWELPEHAMTGLIGKFRPSIPLSKEELEREGIWDFDRLSLPYIRQEFVQERFGASWTF
IVSNFLNDYQKGRYVFKEDCDTEKKIASKLKMLAEKSMLLESEDKIRRDLFDLLKNIVLIRDPE
DESKFYPRFNLEDTSSFQDLDDHSKNVLKRLYYDYYFHRQENLWRQNALKTLPALLDSSDMLAC
GEDLGLIPACVHPVMQELGLIGLRIQRMSSEPDLEFGIPSQYSYMTQWLKPYAAFCFLRDFFET
SDHSQWGRFSCFTEKKVEKLVSKDSLHHDIIRFHYYIQFHLHTQLTEAAEYARKKGVILKGDLP
IGVDRNSVDTWVYPNLFRMNTSTGAPPDYFDKNGQNWGFPTYNWEEMSKDNYAWWRARLTQMAK
YFTAYRIDHILGFFRIWELPEHAMTGLIGKFRPSIPLSKEELEREGIWDFDRLSLPYIRQEFVQ
ERFGASWTFIVSNFLNDYQKGRYVFKEDCDTEKKIASKLKMLAEKSMLLESEDKIRRDLFDLLK
NIVLIRDPEDESKFYPRFNLEDTSSFQDLDDHSKNVLKRLYYDYYFHRQENLWRQNALKTLPAL
LDSSDMLACGEDLGLIPACVHPVMQELGLIGLRIQRMSSEPDLEFGIPSQYSYMTQWLKPYAAF
CFLRDFFETSDHSQWGRFSCFTEKKVEKLVSKDSLHHDIIRFHYYIQFHLHTQLTEAAEYARKK
GVILKGDLPIGVDRNSVDTWVYPNLFRMNTSTGAPPDYFDKNGQNWGFPTYNWEEMSKDNYAWW
RARLTQMAKYFTAYRIDHILGFFRIWELPEHAMTGLIGKFRPSIPLSKEELEREGIWDFDRLSL
PYIRQEFVQERFGASWTFIVSNFLNDYQKGRYVFKEDCDTEKKIASKLKMLAEKSMLLESEDKI
RRDLFDLLKNIVLIRDPEDESKFYPRFNLEDTSSFQDLDDHSKNVLKRLYYDYYFHRQENLWRQ
NALKTLPALLDSSDMLACGEDLGLIPACVHPVMQELGLIGLRIQRMSSEPDLEFGIPSQYSYMT
(-)
						
					

						Sponge-miRNAs
						
							NA						
					

						circRNA-miRNA-mRNA network
						 VISUALIZATION
					

						Potential function description
						NA
					

			
		

Functional Information
Coding potential	Y
Potential coding position	1417172-1414534(+) 1416658-1414442(-)
Potential amino acid sequence	MFQKSPAKNKRLHRALTIASCSCIEKEYQTPGQVHWTYVECVNLSILVLA*(+) MNTSTGAPPDYFDKNGQNWGFPTYNWEEMSKDNYAWWRARLTQMAKYFTAYRIDHILGFFRIWE LPEHAMTGLIGKFRPSIPLSKEELEREGIWDFDRLSLPYIRQEFVQERFGASWTFIVSNFLNDY QKGRYVFKEDCDTEKKIASKLKMLAEKSMLLESEDKIRRDLFDLLKNIVLIRDPEDESKFYPRF NLEDTSSFQDLDDHSKNVLKRLYYDYYFHRQENLWRQNALKTLPALLDSSDMLACGEDLGLIPA CVHPVMQELGLIGLRIQRMSSEPDLEFGIPSQYSYMTQWLKPYAAFCFLRDFFETSDHSQWGRF SCFTEKKVEKLVSKDSLHHDIIRFHYYIQFHLHTQLTEAAEYARKKGVILKGDLPIGVDRNSVD TWVYPNLFRMNTSTGAPPDYFDKNGQNWGFPTYNWEEMSKDNYAWWRARLTQMAKYFTAYRIDH ILGFFRIWELPEHAMTGLIGKFRPSIPLSKEELEREGIWDFDRLSLPYIRQEFVQERFGASWTF IVSNFLNDYQKGRYVFKEDCDTEKKIASKLKMLAEKSMLLESEDKIRRDLFDLLKNIVLIRDPE DESKFYPRFNLEDTSSFQDLDDHSKNVLKRLYYDYYFHRQENLWRQNALKTLPALLDSSDMLAC GEDLGLIPACVHPVMQELGLIGLRIQRMSSEPDLEFGIPSQYSYMTQWLKPYAAFCFLRDFFET SDHSQWGRFSCFTEKKVEKLVSKDSLHHDIIRFHYYIQFHLHTQLTEAAEYARKKGVILKGDLP IGVDRNSVDTWVYPNLFRMNTSTGAPPDYFDKNGQNWGFPTYNWEEMSKDNYAWWRARLTQMAK YFTAYRIDHILGFFRIWELPEHAMTGLIGKFRPSIPLSKEELEREGIWDFDRLSLPYIRQEFVQ ERFGASWTFIVSNFLNDYQKGRYVFKEDCDTEKKIASKLKMLAEKSMLLESEDKIRRDLFDLLK NIVLIRDPEDESKFYPRFNLEDTSSFQDLDDHSKNVLKRLYYDYYFHRQENLWRQNALKTLPAL LDSSDMLACGEDLGLIPACVHPVMQELGLIGLRIQRMSSEPDLEFGIPSQYSYMTQWLKPYAAF CFLRDFFETSDHSQWGRFSCFTEKKVEKLVSKDSLHHDIIRFHYYIQFHLHTQLTEAAEYARKK GVILKGDLPIGVDRNSVDTWVYPNLFRMNTSTGAPPDYFDKNGQNWGFPTYNWEEMSKDNYAWW RARLTQMAKYFTAYRIDHILGFFRIWELPEHAMTGLIGKFRPSIPLSKEELEREGIWDFDRLSL PYIRQEFVQERFGASWTFIVSNFLNDYQKGRYVFKEDCDTEKKIASKLKMLAEKSMLLESEDKI RRDLFDLLKNIVLIRDPEDESKFYPRFNLEDTSSFQDLDDHSKNVLKRLYYDYYFHRQENLWRQ NALKTLPALLDSSDMLACGEDLGLIPACVHPVMQELGLIGLRIQRMSSEPDLEFGIPSQYSYMT (-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Liu et al., 2021

circRNA

Detail information of POPTR_0016s02870_circ_g.1

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