Plant Circular RNA Database

Detail information of Zm00001d027633_circ_g.2

			
						 General Information
					
						CircRNA Name
						Zm00001d027633_circ_g.2
					
						ID in PlantcircBase
						zma_circ_006395
					
						Alias
						zma_circ_0000742, GRMZM2G113174_C1
					
						Organism
						
							Zea mays						
					
						Position
						
							chr1: 9611227-9612279  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ, CIRI2
					
						Parent gene
						
							Zm00001d027633						
					
						Parent gene annotation
						
							Protein PIR
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Zm00001d027633_circ_g.2 Zm00001d027633_circ_igg.1 Zm00001d027633_circ_igg.3 Zm00001d027634_circ_ig.1 Zm00001d027634_circ_ig.2 						
					
						Support reads
						NA
					
						Tissues
						leaf, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d027633_T011:4
Zm00001d027633_T008:4
Zm00001d027633_T009:4
Zm00001d027633_T006:4
Zm00001d027633_T012:4
Zm00001d027633_T007:4
Zm00001d027633_T010:4

General Information
CircRNA Name	Zm00001d027633_circ_g.2
ID in PlantcircBase	zma_circ_006395
Alias	zma_circ_0000742, GRMZM2G113174_C1
Organism	Zea mays
Position	chr1: 9611227-9612279 JBrowse»
Reference genome	AGPv4.38
Type	e-circRNA
Identification method	find_circ, CIRI2
Parent gene	Zm00001d027633
Parent gene annotation	Protein PIR
Parent gene strand	+
Alternative splicing	Zm00001d027633_circ_g.2 Zm00001d027633_circ_igg.1 Zm00001d027633_circ_igg.3 Zm00001d027634_circ_ig.1 Zm00001d027634_circ_ig.2
Support reads	NA
Tissues	leaf, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d027633_T011:4 Zm00001d027633_T008:4 Zm00001d027633_T009:4 Zm00001d027633_T006:4 Zm00001d027633_T012:4 Zm00001d027633_T007:4 Zm00001d027633_T010:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GACATGGGAAGCAAAATCTGAAGTTAAAACTGAAGTTCTTCATGACCTTAAGGAAATTGGAAGT
GCTTTGTATTGGATGAGCATTCTGGACATTGTGTTGgatttgaccatggcataccagggtcttg
ctgggctgtaccgtgacttttttggagtccctcatatgtttgcagttgttaaacttcttggttc
cagatcat

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 390
Transcript exons: 9611227-9611370,9611541-9611628,9611983-9612012,9612152-
9612279
GATTTGACCATGGCATACCAGGGTCTTGCTGGGCTGTACCGTGACTTTTTTGGAGTCCCTCATA
TGTTTGCAGTTGTTAAACTTCTTGGTTCCAGATCATTACCTGCAATCATCCGTGCTTTACTGGA
TCATATATCAAGTAAGATTACAGGTTTGCTACCGAAAATAAATGCACTGCAAGAAGCATTACCA
AAATCTATTGGGCTTCTTTCATTTGATGGTGGTATAGCAGAGTATGGACTAGCTGCAATTTCTA
GTTTTGGTTGCCAAAAGATAGTTCATGAAATTTTGACATGGGAAGCAAAATCTGAAGTTAAAAC
TGAAGTTCTTCATGACCTTAAGGAAATTGGAAGTGCTTTGTATTGGATGAGCATTCTGGACATT
GTGTTG
						
					

						Genomic sequence
						
							GATTTGACCATGGCATACCAGGGTCTTGCTGGGCTGTACCGTGACTTTTTTGGAGTCCCTCATA
TGTTTGCAGTTGTTAAACTTCTTGGTTCCAGATCATTACCTGCAATCATCCGTGCTTTACTGGA
TCATATATCAAGTAAGGTGAGTAGTAAGGAACATTGTATTGTGTTATACACCAGAAGGCACAAA
ATTCAAACAACACAATTCAGTGGATGAACAAGCTTGACATCTTTTCATCCGACAGAAAAGTTGG
AGACACCTAGAAAACAATCAAATTACTATGAAGCTAATGTTCTTTTCTTCCTTTTTAGATTACA
GGTTTGCTACCGAAAATAAATGCACTGCAAGAAGCATTACCAAAATCTATTGGGCTTCTTTCAT
TTGATGGTGGTATAGCAGGTAACATATTAACTGATGGCAATCATGGTTCTGATTTCTAAACCCC
GTTGTGAATGAGTTGTTTCTTTCTTGCTCCCCCAAATGTGGGACTAATACCTTGTTACTACTAC
ACTATCCTTTGTTTATTGTGCAATCAATCCTATGTACTTCTGTTCATTGTACTACAAGGGTAAG
ATATGTGTTTAAAGTACAGTTTTGAACATGCATTAGGCATGATCATCTCCCCAGAACAGAGGTA
TCTTTGCAACTTGTTTTCTTTTATTGTAGGCAATTTGGTGCACATTTTTTAGGCTATTAGCTAT
GGTGTTAGGTTCTAATTTAAACTTTGGTGTACATCTGGTAAATCCATTTTAGAGTATGGACTAG
CTGCAATTTCTAGTTTTGGTAAGTCAATCTCTTAGCCATCCTATACAGCATGTGCTTAGTCAGC
ATTGTTGTGTTGTAAAATCTCTTTGCAATAGTTCCATGCCAAAAGGCATCTTCTCTAAGTTCTA
AGAACAGTGAAATCCTGCCTTTATTGTAGGTTGCCAAAAGATAGTTCATGAAATTTTGACATGG
GAAGCAAAATCTGAAGTTAAAACTGAAGTTCTTCATGACCTTAAGGAAATTGGAAGTGCTTTGT
ATTGGATGAGCATTCTGGACATTGTGTTG
						
					

			
		

Sequences
Splice junction sequence	GACATGGGAAGCAAAATCTGAAGTTAAAACTGAAGTTCTTCATGACCTTAAGGAAATTGGAAGT GCTTTGTATTGGATGAGCATTCTGGACATTGTGTTGgatttgaccatggcataccagggtcttg ctgggctgtaccgtgacttttttggagtccctcatatgtttgcagttgttaaacttcttggttc cagatcat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 390 Transcript exons: 9611227-9611370,9611541-9611628,9611983-9612012,9612152- 9612279 GATTTGACCATGGCATACCAGGGTCTTGCTGGGCTGTACCGTGACTTTTTTGGAGTCCCTCATA TGTTTGCAGTTGTTAAACTTCTTGGTTCCAGATCATTACCTGCAATCATCCGTGCTTTACTGGA TCATATATCAAGTAAGATTACAGGTTTGCTACCGAAAATAAATGCACTGCAAGAAGCATTACCA AAATCTATTGGGCTTCTTTCATTTGATGGTGGTATAGCAGAGTATGGACTAGCTGCAATTTCTA GTTTTGGTTGCCAAAAGATAGTTCATGAAATTTTGACATGGGAAGCAAAATCTGAAGTTAAAAC TGAAGTTCTTCATGACCTTAAGGAAATTGGAAGTGCTTTGTATTGGATGAGCATTCTGGACATT GTGTTG
Genomic sequence	GATTTGACCATGGCATACCAGGGTCTTGCTGGGCTGTACCGTGACTTTTTTGGAGTCCCTCATA TGTTTGCAGTTGTTAAACTTCTTGGTTCCAGATCATTACCTGCAATCATCCGTGCTTTACTGGA TCATATATCAAGTAAGGTGAGTAGTAAGGAACATTGTATTGTGTTATACACCAGAAGGCACAAA ATTCAAACAACACAATTCAGTGGATGAACAAGCTTGACATCTTTTCATCCGACAGAAAAGTTGG AGACACCTAGAAAACAATCAAATTACTATGAAGCTAATGTTCTTTTCTTCCTTTTTAGATTACA GGTTTGCTACCGAAAATAAATGCACTGCAAGAAGCATTACCAAAATCTATTGGGCTTCTTTCAT TTGATGGTGGTATAGCAGGTAACATATTAACTGATGGCAATCATGGTTCTGATTTCTAAACCCC GTTGTGAATGAGTTGTTTCTTTCTTGCTCCCCCAAATGTGGGACTAATACCTTGTTACTACTAC ACTATCCTTTGTTTATTGTGCAATCAATCCTATGTACTTCTGTTCATTGTACTACAAGGGTAAG ATATGTGTTTAAAGTACAGTTTTGAACATGCATTAGGCATGATCATCTCCCCAGAACAGAGGTA TCTTTGCAACTTGTTTTCTTTTATTGTAGGCAATTTGGTGCACATTTTTTAGGCTATTAGCTAT GGTGTTAGGTTCTAATTTAAACTTTGGTGTACATCTGGTAAATCCATTTTAGAGTATGGACTAG CTGCAATTTCTAGTTTTGGTAAGTCAATCTCTTAGCCATCCTATACAGCATGTGCTTAGTCAGC ATTGTTGTGTTGTAAAATCTCTTTGCAATAGTTCCATGCCAAAAGGCATCTTCTCTAAGTTCTA AGAACAGTGAAATCCTGCCTTTATTGTAGGTTGCCAAAAGATAGTTCATGAAATTTTGACATGG GAAGCAAAATCTGAAGTTAAAACTGAAGTTCTTCATGACCTTAAGGAAATTGGAAGTGCTTTGT ATTGGATGAGCATTCTGGACATTGTGTTG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.164738078

Conservation Information
Conserved circRNAs	NA
PMCS	0.164738078

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							9611237-9612276(+)
9611291-9611993(-)
9611239-9612221(-)
9611244-9612229(-)
						
						Potential amino acid sequence
						
							MAYQGLAGLYRDFFGVPHMFAVVKLLGSRSLPAIIRALLDHISSKITGLLPKINALQEALPKSI
GLLSFDGGIAEYGLAAISSFGCQKIVHEILTWEAKSEVKTEVLHDLKEIGSALYWMSILDIVLD
LTMAYQGLAGLYRDFFGVPHMFAVVKLLGSRSLPAIIRALLDHISSKITGLLPKINALQEALPK
SIGLLSFDGGIAEYGLAAISSFGCQKIVHEILTWEAKSEVKTEVLHDLKEIGSALYWMSILDIV
LDLTMAYQGLAGLYRDFFGVPHMFAVVKLLGSRSLPAIIRALLDHISSKITGLLPKINALQEAL
PKSIGLLSFDGGIAEYGLAAISSFGCQKIVHEILTWEAKSEVKTEVLHDLKEIGSALYWMSILD
IVLDLTMAYQGLAGLYRDFFGVPHMFAVVKLLGSRSLPAIIRALLDHISSKITGLLPKINALQE
ALPKSIGLLSFDGGIAEYGLAAISSFGCQKIVHEILTWEAKSEVKTEVLHDLKEIGSALYWMSI
LDIVL(+)
MRDSKKVTVQPSKTLVCHGQIQHNVQNAHPIQSTSNFLKVMKNFSFNFRFCFPCQNFMNYLLAT
KTRNCS*(-)
MVKSNTMSRMLIQYKALPISLRS*(-)
MPWSNPTQCPECSSNTKHFQFP*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to drought stress

Functional Information
Coding potential	Y
Potential coding position	9611237-9612276(+) 9611291-9611993(-) 9611239-9612221(-) 9611244-9612229(-)
Potential amino acid sequence	MAYQGLAGLYRDFFGVPHMFAVVKLLGSRSLPAIIRALLDHISSKITGLLPKINALQEALPKSI GLLSFDGGIAEYGLAAISSFGCQKIVHEILTWEAKSEVKTEVLHDLKEIGSALYWMSILDIVLD LTMAYQGLAGLYRDFFGVPHMFAVVKLLGSRSLPAIIRALLDHISSKITGLLPKINALQEALPK SIGLLSFDGGIAEYGLAAISSFGCQKIVHEILTWEAKSEVKTEVLHDLKEIGSALYWMSILDIV LDLTMAYQGLAGLYRDFFGVPHMFAVVKLLGSRSLPAIIRALLDHISSKITGLLPKINALQEAL PKSIGLLSFDGGIAEYGLAAISSFGCQKIVHEILTWEAKSEVKTEVLHDLKEIGSALYWMSILD IVLDLTMAYQGLAGLYRDFFGVPHMFAVVKLLGSRSLPAIIRALLDHISSKITGLLPKINALQE ALPKSIGLLSFDGGIAEYGLAAISSFGCQKIVHEILTWEAKSEVKTEVLHDLKEIGSALYWMSI LDIVL(+) MRDSKKVTVQPSKTLVCHGQIQHNVQNAHPIQSTSNFLKVMKNFSFNFRFCFPCQNFMNYLLAT KTRNCS(-) MVKSNTMSRMLIQYKALPISLRS(-) MPWSNPTQCPECSSNTKHFQFP*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to drought stress

			
						 Other Information
					
						References
						Ma et al., 2021b;Zhang et al., 2019

circRNA

Detail information of Zm00001d027633_circ_g.2

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