Plant Circular RNA Database

Detail information of Csa2G149450_circ_g.1

			
						 General Information
					
						CircRNA Name
						Csa2G149450_circ_g.1
					
						ID in PlantcircBase
						csa_circ_000974
					
						Alias
						Chr2:8900721_8908142
					
						Organism
						
							Cucumis sativus						
					
						Position
						
							chrChr2: 8900721-8908142  JBrowse»						
					
						Reference genome
						
							Cucumis sativus v1.5						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI2
					
						Parent gene
						
							Csa2G149450						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							Csa2G149450_circ_g.2 						
					
						Support reads
						NA
					
						Tissues
						leaf,root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Csa2G149450.1:5

General Information
CircRNA Name	Csa2G149450_circ_g.1
ID in PlantcircBase	csa_circ_000974
Alias	Chr2:8900721_8908142
Organism	Cucumis sativus
Position	chrChr2: 8900721-8908142 JBrowse»
Reference genome	Cucumis sativus v1.5
Type	e-circRNA
Identification method	CIRI2
Parent gene	Csa2G149450
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	Csa2G149450_circ_g.2
Support reads	NA
Tissues	leaf,root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Csa2G149450.1:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TACTAAACCAAAGACATCTTCAGATGCTGCGTTGAAACTTGTAGCAAGCCAGGTGGCTGTATCT
TCACCGAAGCTGAAAGCGTACTTGTCTAAAAGAGATgatgctgctgtctagctgttcttcaggc
tgcaagtgtggggttttatgtcttaacaaaccatttcagcaccgacccgtgaagaagatgaaac
tggtcaag

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 607
Transcript exons: 8900721-8900820,8905996-8906074,8906195-8906366,8907718-
8907798,8907968-8908142
GATGCTGCTGTCTAGCTGTTCTTCAGGCTGCAAGTGTGGGGTTTTATGTCTTAACAAACCATTT
CAGCACCGACCCGTGAAGAAGATGAAACTGGTCAAGACTGAGAAATGTGGCTCGGGGATTGTAG
CTGATGAAGATATCAAACAAGGAGAGTTTGTAATTGAATATGTTGGAGAAGTAATTGATGATAA
GACATGTGAAGAAAGGCTATGGAACATGAAGCATCGTGGGGAGACAAACTTCTATTTGTGTGAG
ATCAACCGTGATATGGTAATTGATGCCACATACAAGGGAAATAAGTCGAGATATATAAATCACA
GTTGTTGTCCCAATACTGAGATGCAGAAATGGATTATAGATGGAGAAACAAGAATCGGCATATT
TGCAACTCGAGACATACCAAAGGGCGAGCATCTAACTTATGATTATCAGTTTGTTCAGTTTGGT
GCAGATCAAGATTGTCATTGTGGTGCTGTTGACTGCAGAAGAAAGTTGGGTGTAAGACCTACTA
AACCAAAGACATCTTCAGATGCTGCGTTGAAACTTGTAGCAAGCCAGGTGGCTGTATCTTCACC
GAAGCTGAAAGCGTACTTGTCTAAAAGAGAT
						
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	TACTAAACCAAAGACATCTTCAGATGCTGCGTTGAAACTTGTAGCAAGCCAGGTGGCTGTATCT TCACCGAAGCTGAAAGCGTACTTGTCTAAAAGAGATgatgctgctgtctagctgttcttcaggc tgcaagtgtggggttttatgtcttaacaaaccatttcagcaccgacccgtgaagaagatgaaac tggtcaag
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 607 Transcript exons: 8900721-8900820,8905996-8906074,8906195-8906366,8907718- 8907798,8907968-8908142 GATGCTGCTGTCTAGCTGTTCTTCAGGCTGCAAGTGTGGGGTTTTATGTCTTAACAAACCATTT CAGCACCGACCCGTGAAGAAGATGAAACTGGTCAAGACTGAGAAATGTGGCTCGGGGATTGTAG CTGATGAAGATATCAAACAAGGAGAGTTTGTAATTGAATATGTTGGAGAAGTAATTGATGATAA GACATGTGAAGAAAGGCTATGGAACATGAAGCATCGTGGGGAGACAAACTTCTATTTGTGTGAG ATCAACCGTGATATGGTAATTGATGCCACATACAAGGGAAATAAGTCGAGATATATAAATCACA GTTGTTGTCCCAATACTGAGATGCAGAAATGGATTATAGATGGAGAAACAAGAATCGGCATATT TGCAACTCGAGACATACCAAAGGGCGAGCATCTAACTTATGATTATCAGTTTGTTCAGTTTGGT GCAGATCAAGATTGTCATTGTGGTGCTGTTGACTGCAGAAGAAAGTTGGGTGTAAGACCTACTA AACCAAAGACATCTTCAGATGCTGCGTTGAAACTTGTAGCAAGCCAGGTGGCTGTATCTTCACC GAAGCTGAAAGCGTACTTGTCTAAAAGAGAT
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.472651571

Conservation Information
Conserved circRNAs	NA
PMCS	0.472651571

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							8900723-8900732(+)
8908142-8900732(+)
						
						Potential amino acid sequence
						
							MLLSSCSSGCKCGVLCLNKPFQHRPVKKMKLVKTEKCGSGIVADEDIKQGEFVIEYVGEVIDDK
TCEERLWNMKHRGETNFYLCEINRDMVIDATYKGNKSRYINHSCCPNTEMQKWIIDGETRIGIF
ATRDIPKGEHLTYDYQFVQFGADQDCHCGAVDCRRKLGVRPTKPKTSSDAALKLVASQVAVSSP
KLKAYLSKRDDAAV*
MMLLSSCSSGCKCGVLCLNKPFQHRPVKKMKLVKTEKCGSGIVADEDIKQGEFVIEYVGEVIDD
KTCEERLWNMKHRGETNFYLCEINRDMVIDATYKGNKSRYINHSCCPNTEMQKWIIDGETRIGI
FATRDIPKGEHLTYDYQFVQFGADQDCHCGAVDCRRKLGVRPTKPKTSSDAALKLVASQVAVSS
PKLKAYLSKRDDAAV*
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to salt stress in root

Functional Information
Coding potential	Y
Potential coding position	8900723-8900732(+) 8908142-8900732(+)
Potential amino acid sequence	MLLSSCSSGCKCGVLCLNKPFQHRPVKKMKLVKTEKCGSGIVADEDIKQGEFVIEYVGEVIDDK TCEERLWNMKHRGETNFYLCEINRDMVIDATYKGNKSRYINHSCCPNTEMQKWIIDGETRIGIF ATRDIPKGEHLTYDYQFVQFGADQDCHCGAVDCRRKLGVRPTKPKTSSDAALKLVASQVAVSSP KLKAYLSKRDDAAV* MMLLSSCSSGCKCGVLCLNKPFQHRPVKKMKLVKTEKCGSGIVADEDIKQGEFVIEYVGEVIDD KTCEERLWNMKHRGETNFYLCEINRDMVIDATYKGNKSRYINHSCCPNTEMQKWIIDGETRIGI FATRDIPKGEHLTYDYQFVQFGADQDCHCGAVDCRRKLGVRPTKPKTSSDAALKLVASQVAVSS PKLKAYLSKRDDAAV*
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to salt stress in root

			
						 Other Information
					
						References
						Zhu et al., 2019

circRNA

Detail information of Csa2G149450_circ_g.1

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