Plant Circular RNA Database

Detail information of AT2G32640_circ_g.5

			
						 General Information
					
						CircRNA Name
						AT2G32640_circ_g.5
					
						ID in PlantcircBase
						ath_circ_016129
					
						Alias
						At_ciR4118, Ath_circ_FC1463
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr2: 13848757-13849118  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						KNIFE, PcircRNA_finder, find_circ
					
						Parent gene
						
							AT2G32640						
					
						Parent gene annotation
						
							Lycopene beta/epsilon cyclase protein
							
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						4/2
					
						Tissues
						leaf/whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT2G32640.1:2
AT2G32640.2:2

General Information
CircRNA Name	AT2G32640_circ_g.5
ID in PlantcircBase	ath_circ_016129
Alias	At_ciR4118, Ath_circ_FC1463
Organism	Arabidpsis thaliana
Position	chr2: 13848757-13849118 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	KNIFE, PcircRNA_finder, find_circ
Parent gene	AT2G32640
Parent gene annotation	Lycopene beta/epsilon cyclase protein
Parent gene strand	-
Alternative splicing	NA
Support reads	4/2
Tissues	leaf/whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT2G32640.1:2 AT2G32640.2:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AACTGCTATATATAACGTCGCTTGATGAGTTCTCTTTAAACCCATGAGCACAAGATCCAACAAC
AAGGCACATTCCATCTGGCTTTCTACCACGTTTGATttggtattttggcatcagtttccagtat
tcttcgagtaaatcctctaaactcggagatgttgattgcggttcagtgtaagtgaacatgtaag
tagtgcgg

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 276
Transcript exons: 13848757-13848885,13848972-13849118
TTGGTATTTTGGCATCAGTTTCCAGTATTCTTCGAGTAAATCCTCTAAACTCGGAGATGTTGAT
TGCGGTTCAGTGTAAGTGAACATGTAAGTAGTGCGGTCTAATGGACCAGAGCCAGCCGGGAAAG
CCTCCCAAAAGAGTTGTACATTGGAATCTGCAACTCTGGTCACCGATGAACTGCTATATATAAC
GTCGCTTGATGAGTTCTCTTTAAACCCATGAGCACAAGATCCAACAACAAGGCACATTCCATCT
GGCTTTCTACCACGTTTGAT
						
						Genomic sequence
						
							TTGGTATTTTGGCATCAGTTTCCAGTATTCTTCGAGTAAATCCTCTAAACTCGGAGATGTTGAT
TGCGGTTCAGTGTAAGTGAACATGTAAGTAGTGCGGTCTAATGGACCAGAGCCAGCCGGGAAAG
CCTTAAAGCAACAATAAGCATCCTTATATTAAAACCATTTCTTTGTGGTTATGGGAATTGTGTT
AGAATGGGGACAGATTTTTTTACCTCCCAAAAGAGTTGTACATTGGAATCTGCAACTCTGGTCA
CCGATGAACTGCTATATATAACGTCGCTTGATGAGTTCTCTTTAAACCCATGAGCACAAGATCC
AACAACAAGGCACATTCCATCTGGCTTTCTACCACGTTTGAT

Sequences
Splice junction sequence	AACTGCTATATATAACGTCGCTTGATGAGTTCTCTTTAAACCCATGAGCACAAGATCCAACAAC AAGGCACATTCCATCTGGCTTTCTACCACGTTTGATttggtattttggcatcagtttccagtat tcttcgagtaaatcctctaaactcggagatgttgattgcggttcagtgtaagtgaacatgtaag tagtgcgg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 276 Transcript exons: 13848757-13848885,13848972-13849118 TTGGTATTTTGGCATCAGTTTCCAGTATTCTTCGAGTAAATCCTCTAAACTCGGAGATGTTGAT TGCGGTTCAGTGTAAGTGAACATGTAAGTAGTGCGGTCTAATGGACCAGAGCCAGCCGGGAAAG CCTCCCAAAAGAGTTGTACATTGGAATCTGCAACTCTGGTCACCGATGAACTGCTATATATAAC GTCGCTTGATGAGTTCTCTTTAAACCCATGAGCACAAGATCCAACAACAAGGCACATTCCATCT GGCTTTCTACCACGTTTGAT
Genomic sequence	TTGGTATTTTGGCATCAGTTTCCAGTATTCTTCGAGTAAATCCTCTAAACTCGGAGATGTTGAT TGCGGTTCAGTGTAAGTGAACATGTAAGTAGTGCGGTCTAATGGACCAGAGCCAGCCGGGAAAG CCTTAAAGCAACAATAAGCATCCTTATATTAAAACCATTTCTTTGTGGTTATGGGAATTGTGTT AGAATGGGGACAGATTTTTTTACCTCCCAAAAGAGTTGTACATTGGAATCTGCAACTCTGGTCA CCGATGAACTGCTATATATAACGTCGCTTGATGAGTTCTCTTTAAACCCATGAGCACAAGATCC AACAACAAGGCACATTCCATCTGGCTTTCTACCACGTTTGAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.26287026

Conservation Information
Conserved circRNAs	NA
PMCS	0.26287026

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							13849092-13848759(-)
						
						Potential amino acid sequence
						
							MCLVVGSCAHGFKENSSSDVIYSSSSVTRVADSNVQLFWEAFPAGSGPLDRTTYMFTYTEPQST
SPSLEDLLEEYWKLMPKYQIKRGRKPDGMCLVVGSCAHGFKENSSSDVIYSSSSVTRVADSNVQ
LFWEAFPAGSGPLDRTTYMFTYTEPQSTSPSLEDLLEEYWKLMPKYQIKRGRKPDGMCLVVGSC
AHGFKENSSSDVIYSSSSVTRVADSNVQLFWEAFPAGSGPLDRTTYMFTYTEPQSTSPSLEDLL
EEYWKLMPKYQIKRGRKPDGMCLVVGSCAHGFKENSSSDVIYSSSSVTRVADSNVQLFWEAFPA
GSGPLDRTTYMFTYTEPQSTSPSLEDLLEEYWKLMPKYQ(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	13849092-13848759(-)
Potential amino acid sequence	MCLVVGSCAHGFKENSSSDVIYSSSSVTRVADSNVQLFWEAFPAGSGPLDRTTYMFTYTEPQST SPSLEDLLEEYWKLMPKYQIKRGRKPDGMCLVVGSCAHGFKENSSSDVIYSSSSVTRVADSNVQ LFWEAFPAGSGPLDRTTYMFTYTEPQSTSPSLEDLLEEYWKLMPKYQIKRGRKPDGMCLVVGSC AHGFKENSSSDVIYSSSSVTRVADSNVQLFWEAFPAGSGPLDRTTYMFTYTEPQSTSPSLEDLL EEYWKLMPKYQIKRGRKPDGMCLVVGSCAHGFKENSSSDVIYSSSSVTRVADSNVQLFWEAFPA GSGPLDRTTYMFTYTEPQSTSPSLEDLLEEYWKLMPKYQ(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017;Chen et al., 2017a

circRNA

Detail information of AT2G32640_circ_g.5

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