Plant Circular RNA Database

Detail information of Os02g0778300_circ_g.6

			
						 General Information
					
						CircRNA Name
						Os02g0778300_circ_g.6
					
						ID in PlantcircBase
						osa_circ_016774
					
						Alias
						Os_ciR3785, 
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 32948236-32949484  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ, CIRI-long
					
						Parent gene
						
							Os02g0778300						
					
						Parent gene annotation
						
							Conserved hypothetical protein. (Os02t0778300-01);Similar to pre
dicted protein. (Os02t0778300-02);Non-protein coding transcript.
 (Os02t0778300-03)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os02g0778300_circ_g.1 Os02g0778300_circ_g.2 Os02g0778300_circ_g.3 Os02g0778300_circ_g.4 Os02g0778300_circ_g.5 Os02g0778300_circ_g.7 Os02g0778300_circ_g.8 Os02g0778300_circ_g.9 Os02g0778300_circ_g.10 						
					
						Support reads
						2
					
						Tissues
						root, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0778300-02:4
Os02t0778300-01:4

General Information
CircRNA Name	Os02g0778300_circ_g.6
ID in PlantcircBase	osa_circ_016774
Alias	Os_ciR3785,
Organism	Oryza sativa
Position	chr2: 32948236-32949484 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	find_circ, CIRI-long
Parent gene	Os02g0778300
Parent gene annotation	Conserved hypothetical protein. (Os02t0778300-01);Similar to pre dicted protein. (Os02t0778300-02);Non-protein coding transcript. (Os02t0778300-03)
Parent gene strand	+
Alternative splicing	Os02g0778300_circ_g.1 Os02g0778300_circ_g.2 Os02g0778300_circ_g.3 Os02g0778300_circ_g.4 Os02g0778300_circ_g.5 Os02g0778300_circ_g.7 Os02g0778300_circ_g.8 Os02g0778300_circ_g.9 Os02g0778300_circ_g.10
Support reads	2
Tissues	root, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0778300-02:4 Os02t0778300-01:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GAATTGATGGACTTGCATCAACCCCCTTTTAATTCTACAGGCCGATCTTGCTCATCTATTAGCA
TCGCGTGATCAAGAACTTAGAGCACTCTCCGCTGAGggcatatccacaagtacaactctctgaa
tgggcaccctgatattttgcggtccttatattctgcactccaactccaaccatctagttctttg
gaagaacg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 308
Transcript exons: 32948236-32948338,32948884-32948965,32949224-32949286,32
949425-32949484
GGCATATCCACAAGTACAACTCTCTGAATGGGCACCCTGATATTTTGCGGTCCTTATATTCTGC
ACTCCAACTCCAACCATCTAGTTCTTTGGAAGAACGAAGGTATTATGATGGTCGTTTTTCTGAC
CAGCAAATGGCTCTGCTTCAGTACCAAAGGGAGAATATTCATTACCTGAGTGAGGAGGTGCTCC
GCTTGCAAGAATGCTTAAGCAAATACCAGAGAACTGATGTTGGGAGCACTCCTCAGGCCGATCT
TGCTCATCTATTAGCATCGCGTGATCAAGAACTTAGAGCACTCTCCGCTGAG
						
					

						Genomic sequence
						
							GGCATATCCACAAGTACAACTCTCTGAATGGGCACCCTGATATTTTGCGGTCCTTATATTCTGC
ACTCCAACTCCAACCATCTAGTTCTTTGGAAGAACGAAGGTACAACATAAGGGTGTACATTAAA
TGCATTGAACTTTTCCTTCTTAATTTACAATAAAATACTACCTCCGTTTTTTAATAGATGACGC
CGTTGACTTTTTCTCACATGTTTGACCATTCGTCTTATTCAAAAAAATTACGTAATTATAATTT
ATTTTGTTATGAGTTGTTTTATCACTCATAGTACTTTAAGTGTGATTTATATCTTATACATTTG
CATAAAATTTCTGAATAAGACGAATGGTCAAACATGTGAGAAAAAGTCAACGGCGTCATCTATT
AAAAAATGGAGGGAGTAGTGTTTGAAGACACGTATAAACATGTACTATGCACAGTTTCTACCAC
TTCAGGCTTGGCGTTTGATTTTCTGGCAGTTTGGAAAAGCATTCTTTAGTCCATGTTTATCAGA
AACATCTGTATTGAACCTATATCTAGAAGGATGTGCCTTCACATGAGTTACAAGTGTTGATATG
TTGCCGAATATGTCATATGTGTACTTGGCATACTGCCAGTAGAGTTTCACAAGTTATTTTTCTT
TTCACCAGGTATTATGATGGTCGTTTTTCTGACCAGCAAATGGCTCTGCTTCAGTACCAAAGGG
AGAATATTCATTACCTGAGTGAGGAGGTACTTGGAAGATCAATGCAGTATATCCATTTTAAAAT
TTGATTATGTTAGATTGTGTTATTATTTAAATATTTGTACAAGACAACAGTAGATTACTAGCAT
TACTATTTCATGGAGGAATTAGCTGATCCTTGTATTTAGTTCTACATTAACTTAATTGGCCAGT
TCTGTAGTCAGTGCTGAAGTTCTCAATAATAATATGTTGGAACTTCCAGAAAATTTCTTGTTGA
ATAACCAAACCTTTTATTTTCCTGAAAGGTGCTCCGCTTGCAAGAATGCTTAAGCAAATACCAG
AGAACTGATGTTGGGAGCACTCCTCAGGTCTGCACAACACTGATTGATTGTGTTGCATTAATTA
ACTGACCAGCTTCGTAGGTGCAAAATTAGAGAAAGGAACTTTGTAGCTATTATGTTTATGTGAA
TTGATGGACTTGCATCAACCCCCTTTTAATTCTACAGGCCGATCTTGCTCATCTATTAGCATCG
CGTGATCAAGAACTTAGAGCACTCTCCGCTGAG
						
					

			
		

Sequences
Splice junction sequence	GAATTGATGGACTTGCATCAACCCCCTTTTAATTCTACAGGCCGATCTTGCTCATCTATTAGCA TCGCGTGATCAAGAACTTAGAGCACTCTCCGCTGAGggcatatccacaagtacaactctctgaa tgggcaccctgatattttgcggtccttatattctgcactccaactccaaccatctagttctttg gaagaacg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 308 Transcript exons: 32948236-32948338,32948884-32948965,32949224-32949286,32 949425-32949484 GGCATATCCACAAGTACAACTCTCTGAATGGGCACCCTGATATTTTGCGGTCCTTATATTCTGC ACTCCAACTCCAACCATCTAGTTCTTTGGAAGAACGAAGGTATTATGATGGTCGTTTTTCTGAC CAGCAAATGGCTCTGCTTCAGTACCAAAGGGAGAATATTCATTACCTGAGTGAGGAGGTGCTCC GCTTGCAAGAATGCTTAAGCAAATACCAGAGAACTGATGTTGGGAGCACTCCTCAGGCCGATCT TGCTCATCTATTAGCATCGCGTGATCAAGAACTTAGAGCACTCTCCGCTGAG
Genomic sequence	GGCATATCCACAAGTACAACTCTCTGAATGGGCACCCTGATATTTTGCGGTCCTTATATTCTGC ACTCCAACTCCAACCATCTAGTTCTTTGGAAGAACGAAGGTACAACATAAGGGTGTACATTAAA TGCATTGAACTTTTCCTTCTTAATTTACAATAAAATACTACCTCCGTTTTTTAATAGATGACGC CGTTGACTTTTTCTCACATGTTTGACCATTCGTCTTATTCAAAAAAATTACGTAATTATAATTT ATTTTGTTATGAGTTGTTTTATCACTCATAGTACTTTAAGTGTGATTTATATCTTATACATTTG CATAAAATTTCTGAATAAGACGAATGGTCAAACATGTGAGAAAAAGTCAACGGCGTCATCTATT AAAAAATGGAGGGAGTAGTGTTTGAAGACACGTATAAACATGTACTATGCACAGTTTCTACCAC TTCAGGCTTGGCGTTTGATTTTCTGGCAGTTTGGAAAAGCATTCTTTAGTCCATGTTTATCAGA AACATCTGTATTGAACCTATATCTAGAAGGATGTGCCTTCACATGAGTTACAAGTGTTGATATG TTGCCGAATATGTCATATGTGTACTTGGCATACTGCCAGTAGAGTTTCACAAGTTATTTTTCTT TTCACCAGGTATTATGATGGTCGTTTTTCTGACCAGCAAATGGCTCTGCTTCAGTACCAAAGGG AGAATATTCATTACCTGAGTGAGGAGGTACTTGGAAGATCAATGCAGTATATCCATTTTAAAAT TTGATTATGTTAGATTGTGTTATTATTTAAATATTTGTACAAGACAACAGTAGATTACTAGCAT TACTATTTCATGGAGGAATTAGCTGATCCTTGTATTTAGTTCTACATTAACTTAATTGGCCAGT TCTGTAGTCAGTGCTGAAGTTCTCAATAATAATATGTTGGAACTTCCAGAAAATTTCTTGTTGA ATAACCAAACCTTTTATTTTCCTGAAAGGTGCTCCGCTTGCAAGAATGCTTAAGCAAATACCAG AGAACTGATGTTGGGAGCACTCCTCAGGTCTGCACAACACTGATTGATTGTGTTGCATTAATTA ACTGACCAGCTTCGTAGGTGCAAAATTAGAGAAAGGAACTTTGTAGCTATTATGTTTATGTGAA TTGATGGACTTGCATCAACCCCCTTTTAATTCTACAGGCCGATCTTGCTCATCTATTAGCATCG CGTGATCAAGAACTTAGAGCACTCTCCGCTGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.119684154

Conservation Information
Conserved circRNAs	NA
PMCS	0.119684154

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							32948916-32948259(+)
32948318-32949445(-)
						
						Potential amino acid sequence
						
							MALLQYQRENIHYLSEEVLRLQECLSKYQRTDVGSTPQADLAHLLASRDQELRALSAEGISTST
TL*(+)
MVGVGVQNIRTAKYQGAHSESCTCGYALSGECSKFLITRC*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	32948916-32948259(+) 32948318-32949445(-)
Potential amino acid sequence	MALLQYQRENIHYLSEEVLRLQECLSKYQRTDVGSTPQADLAHLLASRDQELRALSAEGISTST TL(+) MVGVGVQNIRTAKYQGAHSESCTCGYALSGECSKFLITRC(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015; this study

circRNA

Detail information of Os02g0778300_circ_g.6

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