Plant Circular RNA Database

Detail information of orai.004G027600_circ_g.1

			
						 General Information
					
						CircRNA Name
						orai.004G027600_circ_g.1
					
						ID in PlantcircBase
						gra_circ_000317
					
						Alias
						Chr04:2165458|2167364
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr04: 2165458-2167364  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.004G027600						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						3/8
					
						Tissues
						leaf/ovule
					
						Exon boundary  
						
							Yes-No							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.004G027600.4:1
Gorai.004G027600.5:1
Gorai.004G027600.3:2
Gorai.004G027600.1:2
Gorai.004G027600.2:2

General Information
CircRNA Name	orai.004G027600_circ_g.1
ID in PlantcircBase	gra_circ_000317
Alias	Chr04:2165458\|2167364
Organism	Gossypium raimondii
Position	chrChr04: 2165458-2167364 JBrowse»
Reference genome	Graimondii_221
Type	ue-circRNA
Identification method	CIRI
Parent gene	Gorai.004G027600
Parent gene annotation	NA
Parent gene strand	-
Alternative splicing	NA
Support reads	3/8
Tissues	leaf/ovule
Exon boundary	Yes-No "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.004G027600.4:1 Gorai.004G027600.5:1 Gorai.004G027600.3:2 Gorai.004G027600.1:2 Gorai.004G027600.2:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							ACCCCAAATTTTTCTTTACCTTCAAAAGAGGAATCCATGGCAATAGGAAAGCACTCAACACAAA
ACCCACATTACAATCAAAACCCCAAATTTTCTAAGCccaagcaaatcatttactttcccacatg
ctacacaataaaagctcccatcaagaacggcatgttgtttatctttaccaataccggacttttc
atgtttta

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							CCAAGCAAATCATTTACTTTCCCACATGCTACACAATAAAAGCTCCCATCAAGAACGGCATGTT
GTTTATCTTTACCAATACCGGACTTTTCATGTTTTAAAGCACACTCGAGATGACACGACATGCC
ACAAGAGTTTCCCAGGAAAGGAGGCTCCGAACTACAAATTAACCATAGACTAGGATCCTTATTG
TCATCGAACTTATAACAGATGCAGCACGAACACCTCTTGCAAAATGTATCGTCTTGTCTCAAGG
TAGCTTTGCAAGCCGAGTTTTTGCAATAAATAGCATTAATCATGTCATTGCCGCCATTACGGAT
TACAGGATCATTCACTGGAATAGGAAGTCGAGAAGGATAATCGGCTTTCCTTTGCCTTTTGGAT
AATCTCGAGCCACTTGATAGGGATGAATGTGATTCGGGATCCGTCACACCTTCATGATCCCCTG
AATTCTTTTCGGCTACAATTTTCAACAAGTTTTCAATAATTTTAAGCTTAGTCAAACCGGTATA
TTTCCTTTCCTTCCCCATCTCGGCACATAGGACTTGTAAAATCTCTTGGCGGCTCCATGATTGT
AGCATTTCAGAGGCTCTACTTGTCCTCTTTGATAATTCATATACGAGTTCTCGCTTTTCATCCA
TGCTCAATTTACTACATTTTGATGGATCGAGCGCAACTCCTAAAGCCAACGGAAAAACATAAAA
TTGCGAGTTAACAAGAGTTAGCACAAGAAAGTCCGAAAGGGACAAAAGATCCTAATACTAATAT
CAACTCGACAAAATGTCAGAAAAATACAAGAAATTTGTCATCCACTTCACACAAAACAACGGCA
ATACTGACAAGATTACCAAATTTAGAACAAGAAAAAAAAAATCAACAAGAACTCGAGAATTGCC
GATACGTAGTCAGTGTTATCAACCAATTATGGAAGTCATTCAGGAAGGTCGAATGATCTATATT
AATCGAAGTAAATTCCGATCCACTCTGTTCTAAAACAGGAAAACTCATAATTGACTTGCATTAG
AAAAAGTACATTCCGAACTATACCGAACACCGTGGATTACTAACATCACAATTCAAGGAAGGGA
TTGAACACAATGCTATTTGACCTTTTACAACCTATTGCCATTCACTTTTGTCTATATATATATA
TACACACGGACACAAGTACGGGGACATGCCTTGGAGAGGATATATATATATATACAGATACATA
TACATACACACACATAAGTGTGAACGAACATGAGTATAAAGACGTGACTCTCCAGGGGAAAAAA
TCAAACATACTCATGTCCAACACATAGCTGTATTCCAACATAAACCGAAGGCCAAGTATACATC
AATATAATGCAATGAATGAAAGTAAATGAACAGTCAAATTCATCCACCAGTTGACCAAAGTGAA
TTAGTAAGAAACTAATGTATCAAATATGATGAAGAAATGTAAAAAAAGACAATATGGTACCAAA
TATCCACAAAAGGAAATTTTTTTTTTTTTAAAAAAAAGCATCTTTTAGCTGAAAACTAAGTTTA
AAGAGAAAATCTTTACTGAAGTGAAAATAAAAATTAAAAAAATTAAAAATAAAAACTGAAACAA
TTTGACCAGCATGCAAAAGATCTAACCTTTAAGACCTTAAATAAACTATAAATGAGAAACCAAA
AAAAATTCATATCCCAGATTACATATCACTAAGAAAATCCAAAGAAGGAAAAAAAATTACATTC
GAAAGAGGAATCCTTAGAAATAATAGCTAAAGCAATAAGAAAGCACTAAAACCCAGATTACATA
TCACTCAAAAAAAAAACCCCAAATTTTTCTTTACCTTCAAAAGAGGAATCCATGGCAATAGGAA
AGCACTCAACACAAAACCCACATTACAATCAAAACCCCAAATTTTCTAAGC
						
					

			
		

Sequences
Splice junction sequence	ACCCCAAATTTTTCTTTACCTTCAAAAGAGGAATCCATGGCAATAGGAAAGCACTCAACACAAA ACCCACATTACAATCAAAACCCCAAATTTTCTAAGCccaagcaaatcatttactttcccacatg ctacacaataaaagctcccatcaagaacggcatgttgtttatctttaccaataccggacttttc atgtttta
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	CCAAGCAAATCATTTACTTTCCCACATGCTACACAATAAAAGCTCCCATCAAGAACGGCATGTT GTTTATCTTTACCAATACCGGACTTTTCATGTTTTAAAGCACACTCGAGATGACACGACATGCC ACAAGAGTTTCCCAGGAAAGGAGGCTCCGAACTACAAATTAACCATAGACTAGGATCCTTATTG TCATCGAACTTATAACAGATGCAGCACGAACACCTCTTGCAAAATGTATCGTCTTGTCTCAAGG TAGCTTTGCAAGCCGAGTTTTTGCAATAAATAGCATTAATCATGTCATTGCCGCCATTACGGAT TACAGGATCATTCACTGGAATAGGAAGTCGAGAAGGATAATCGGCTTTCCTTTGCCTTTTGGAT AATCTCGAGCCACTTGATAGGGATGAATGTGATTCGGGATCCGTCACACCTTCATGATCCCCTG AATTCTTTTCGGCTACAATTTTCAACAAGTTTTCAATAATTTTAAGCTTAGTCAAACCGGTATA TTTCCTTTCCTTCCCCATCTCGGCACATAGGACTTGTAAAATCTCTTGGCGGCTCCATGATTGT AGCATTTCAGAGGCTCTACTTGTCCTCTTTGATAATTCATATACGAGTTCTCGCTTTTCATCCA TGCTCAATTTACTACATTTTGATGGATCGAGCGCAACTCCTAAAGCCAACGGAAAAACATAAAA TTGCGAGTTAACAAGAGTTAGCACAAGAAAGTCCGAAAGGGACAAAAGATCCTAATACTAATAT CAACTCGACAAAATGTCAGAAAAATACAAGAAATTTGTCATCCACTTCACACAAAACAACGGCA ATACTGACAAGATTACCAAATTTAGAACAAGAAAAAAAAAATCAACAAGAACTCGAGAATTGCC GATACGTAGTCAGTGTTATCAACCAATTATGGAAGTCATTCAGGAAGGTCGAATGATCTATATT AATCGAAGTAAATTCCGATCCACTCTGTTCTAAAACAGGAAAACTCATAATTGACTTGCATTAG AAAAAGTACATTCCGAACTATACCGAACACCGTGGATTACTAACATCACAATTCAAGGAAGGGA TTGAACACAATGCTATTTGACCTTTTACAACCTATTGCCATTCACTTTTGTCTATATATATATA TACACACGGACACAAGTACGGGGACATGCCTTGGAGAGGATATATATATATATACAGATACATA TACATACACACACATAAGTGTGAACGAACATGAGTATAAAGACGTGACTCTCCAGGGGAAAAAA TCAAACATACTCATGTCCAACACATAGCTGTATTCCAACATAAACCGAAGGCCAAGTATACATC AATATAATGCAATGAATGAAAGTAAATGAACAGTCAAATTCATCCACCAGTTGACCAAAGTGAA TTAGTAAGAAACTAATGTATCAAATATGATGAAGAAATGTAAAAAAAGACAATATGGTACCAAA TATCCACAAAAGGAAATTTTTTTTTTTTTAAAAAAAAGCATCTTTTAGCTGAAAACTAAGTTTA AAGAGAAAATCTTTACTGAAGTGAAAATAAAAATTAAAAAAATTAAAAATAAAAACTGAAACAA TTTGACCAGCATGCAAAAGATCTAACCTTTAAGACCTTAAATAAACTATAAATGAGAAACCAAA AAAAATTCATATCCCAGATTACATATCACTAAGAAAATCCAAAGAAGGAAAAAAAATTACATTC GAAAGAGGAATCCTTAGAAATAATAGCTAAAGCAATAAGAAAGCACTAAAACCCAGATTACATA TCACTCAAAAAAAAAACCCCAAATTTTTCTTTACCTTCAAAAGAGGAATCCATGGCAATAGGAA AGCACTCAACACAAAACCCACATTACAATCAAAACCCCAAATTTTCTAAGC

			
						 Conservation Information
					
						Conserved circRNAs
						
							ghi_circ_000980* gar_circ_000555* 						
					
						PMCS
						0.954040443

Conservation Information
Conserved circRNAs	ghi_circ_000980* gar_circ_000555*
PMCS	0.954040443

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							2167303-2167321(-)
2166097-2165555(+)
						
						Potential amino acid sequence
						
							MDSSFEGVALDPSKCSKLSMDEKRELVYELSKRTSRASEMLQSWSRQEILQVLCAEMGKERKYT
GLTKLKIIENLLKIVAEKNSGDHEGVTDPESHSSLSSGSRLSKRQRKADYPSRLPIPVNDPVIR
NGGNDMINAIYCKNSACKATLRQDDTFCKRCSCCICYKFDDNKDPSLWLICSSEPPFLGNSCGM
SCHLECALKHEKSGIGKDKQHAVLDGSFYCVACGKVNDLLGLRKFGVLIVMWVLC*(-)
MLNLLHFDGSSATPSKEESMAIGKHSTQNPHYNQNPKFSKPKQIIYFPTCYTIKAPIKNGMLFI
FTNTGLFMF*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	2167303-2167321(-) 2166097-2165555(+)
Potential amino acid sequence	MDSSFEGVALDPSKCSKLSMDEKRELVYELSKRTSRASEMLQSWSRQEILQVLCAEMGKERKYT GLTKLKIIENLLKIVAEKNSGDHEGVTDPESHSSLSSGSRLSKRQRKADYPSRLPIPVNDPVIR NGGNDMINAIYCKNSACKATLRQDDTFCKRCSCCICYKFDDNKDPSLWLICSSEPPFLGNSCGM SCHLECALKHEKSGIGKDKQHAVLDGSFYCVACGKVNDLLGLRKFGVLIVMWVLC(-) MLNLLHFDGSSATPSKEESMAIGKHSTQNPHYNQNPKFSKPKQIIYFPTCYTIKAPIKNGMLFI FTNTGLFMF(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.004G027600_circ_g.1

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