Plant Circular RNA Database

Detail information of Csa3G253520_circ_g.5

			
						 General Information
					
						CircRNA Name
						Csa3G253520_circ_g.5
					
						ID in PlantcircBase
						csa_circ_001727
					
						Alias
						Chr3:15801629_15801964
					
						Organism
						
							Cucumis sativus						
					
						Position
						
							chrChr3: 15801629-15801964  JBrowse»						
					
						Reference genome
						
							Cucumis sativus v1.5						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI2
					
						Parent gene
						
							Csa3G253520						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						-
					
						Alternative splicing
						
							Csa3G253520_circ_g.1 Csa3G253520_circ_g.2 Csa3G253520_circ_g.3 Csa3G253520_circ_g.4 Chr3_circ_ig.54 						
					
						Support reads
						NA
					
						Tissues
						leaf,root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Csa3G253520.1:2

General Information
CircRNA Name	Csa3G253520_circ_g.5
ID in PlantcircBase	csa_circ_001727
Alias	Chr3:15801629_15801964
Organism	Cucumis sativus
Position	chrChr3: 15801629-15801964 JBrowse»
Reference genome	Cucumis sativus v1.5
Type	e-circRNA
Identification method	CIRI2
Parent gene	Csa3G253520
Parent gene annotation	NA
Parent gene strand	-
Alternative splicing	Csa3G253520_circ_g.1 Csa3G253520_circ_g.2 Csa3G253520_circ_g.3 Csa3G253520_circ_g.4 Chr3_circ_ig.54
Support reads	NA
Tissues	leaf,root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Csa3G253520.1:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CTTTGATCTCATTTGAAGGTCTAGCCGCCAACATGGTCACCAGTTGTGTTATTACAGAGAAATC
AAAGCTTTGACCCTCCTGTAGGACTACCTCAGCGACagcaagggtctggcatctgtgtgaaaag
cagaaattgacctaaagtaagatccaacaacttctgccagtgatctataaacctggagtggcat
ttttaaac

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 194
Transcript exons: 15801629-15801708,15801851-15801964
AGCAAGGGTCTGGCATCTGTGTGAAAAGCAGAAATTGACCTAAAGTAAGATCCAACAACTTCTG
CCAGTGATCTATAAACAAGGCACATTAGGCCTTTGATCTCATTTGAAGGTCTAGCCGCCAACAT
GGTCACCAGTTGTGTTATTACAGAGAAATCAAAGCTTTGACCCTCCTGTAGGACTACCTCAGCG
AC
						
						Genomic sequence
						
							AGCAAGGGTCTGGCATCTGTGTGAAAAGCAGAAATTGACCTAAAGTAAGATCCAACAACTTCTG
CCAGTGATCTATAAACCTGGAGTGGCATTTTTAAACAATAAAAGAGTTAGATATATGATAGTAC
TGTGCAACTTCTGGTTTATGAGGCCATAAAGCTTCCCATGAGGTCTAGATAAACTAAGCTATTC
ACACTCGAGAAGTGTAAGGTACCAACATACAAGGCACATTAGGCCTTTGATCTCATTTGAAGGT
CTAGCCGCCAACATGGTCACCAGTTGTGTTATTACAGAGAAATCAAAGCTTTGACCCTCCTGTA
GGACTACCTCAGCGAC

Sequences
Splice junction sequence	CTTTGATCTCATTTGAAGGTCTAGCCGCCAACATGGTCACCAGTTGTGTTATTACAGAGAAATC AAAGCTTTGACCCTCCTGTAGGACTACCTCAGCGACagcaagggtctggcatctgtgtgaaaag cagaaattgacctaaagtaagatccaacaacttctgccagtgatctataaacctggagtggcat ttttaaac
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 194 Transcript exons: 15801629-15801708,15801851-15801964 AGCAAGGGTCTGGCATCTGTGTGAAAAGCAGAAATTGACCTAAAGTAAGATCCAACAACTTCTG CCAGTGATCTATAAACAAGGCACATTAGGCCTTTGATCTCATTTGAAGGTCTAGCCGCCAACAT GGTCACCAGTTGTGTTATTACAGAGAAATCAAAGCTTTGACCCTCCTGTAGGACTACCTCAGCG AC
Genomic sequence	AGCAAGGGTCTGGCATCTGTGTGAAAAGCAGAAATTGACCTAAAGTAAGATCCAACAACTTCTG CCAGTGATCTATAAACCTGGAGTGGCATTTTTAAACAATAAAAGAGTTAGATATATGATAGTAC TGTGCAACTTCTGGTTTATGAGGCCATAAAGCTTCCCATGAGGTCTAGATAAACTAAGCTATTC ACACTCGAGAAGTGTAAGGTACCAACATACAAGGCACATTAGGCCTTTGATCTCATTTGAAGGT CTAGCCGCCAACATGGTCACCAGTTGTGTTATTACAGAGAAATCAAAGCTTTGACCCTCCTGTA GGACTACCTCAGCGAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.988343254

Conservation Information
Conserved circRNAs	NA
PMCS	0.988343254

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							15801900-15801954(-)
						
						Potential amino acid sequence
						
							MLAARPSNEIKGLMCLVYRSLAEVVGSYFRSISAFHTDARPLLSLR*
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to salt stress in root

Functional Information
Coding potential	Y
Potential coding position	15801900-15801954(-)
Potential amino acid sequence	MLAARPSNEIKGLMCLVYRSLAEVVGSYFRSISAFHTDARPLLSLR*
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to salt stress in root

			
						 Other Information
					
						References
						Zhu et al., 2019

circRNA

Detail information of Csa3G253520_circ_g.5

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