Plant Circular RNA Database

Detail information of Os01g0296100_circ_g.4

			
						 General Information
					
						CircRNA Name
						Os01g0296100_circ_g.4
					
						ID in PlantcircBase
						osa_circ_001437
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 10824779-10825051  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os01g0296100						
					
						Parent gene annotation
						
							Similar to Shaggy-related protein kinase alpha (EC 2.7.1.-) (ASK
-alpha). (Os01t0296100-01);Similar to Glycogen synthase kinase. 
(Os01t0296100-02);Similar to Glycogen synthase kinase. (Os01t029
6100-03)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os01g0296100_circ_g.5 Os01g0296100_circ_g.6 Os01g0296100_circ_g.7 						
					
						Support reads
						3
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0296100-01:1
Os01t0296100-02:1
Os01t0296100-03:1

General Information
CircRNA Name	Os01g0296100_circ_g.4
ID in PlantcircBase	osa_circ_001437
Alias	NA
Organism	Oryza sativa
Position	chr1: 10824779-10825051 JBrowse»
Reference genome	IRGSP-1.0.38
Type	u-circRNA
Identification method	CIRCexplorer
Parent gene	Os01g0296100
Parent gene annotation	Similar to Shaggy-related protein kinase alpha (EC 2.7.1.-) (ASK -alpha). (Os01t0296100-01);Similar to Glycogen synthase kinase. (Os01t0296100-02);Similar to Glycogen synthase kinase. (Os01t029 6100-03)
Parent gene strand	+
Alternative splicing	Os01g0296100_circ_g.5 Os01g0296100_circ_g.6 Os01g0296100_circ_g.7
Support reads	3
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0296100-01:1 Os01t0296100-02:1 Os01t0296100-03:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCTTGAATATGTGCCAGAAACTGTTCATCGTGTTATTAGACATTACAATAAGATGAGCCAACGC
ATGCCATTGATTTATGTTAAACTTTATATGTACCAGgctaagtgtctagaaactggtgagagag
ttgctgtaaagaaagttcttcaagatgcgagatacaagaaccgggagctgcaaacgatgcaagt
tctcgatc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 273
Transcript exons: 10824779-10825051
GCTAAGTGTCTAGAAACTGGTGAGAGAGTTGCTGTAAAGAAAGTTCTTCAAGATGCGAGATACA
AGAACCGGGAGCTGCAAACGATGCAAGTTCTCGATCACCCAAATGTTGCATGCTTGAAGCATTA
CTTTTGCTCGACTACTGCGAAGGAGGAGCTATACCTCAACTTGGTTCTTGAATATGTGCCAGAA
ACTGTTCATCGTGTTATTAGACATTACAATAAGATGAGCCAACGCATGCCATTGATTTATGTTA
AACTTTATATGTACCAG
						
						Genomic sequence
						
							GCTAAGTGTCTAGAAACTGGTGAGAGAGTTGCTGTAAAGAAAGTTCTTCAAGATGCGAGATACA
AGAACCGGGAGCTGCAAACGATGCAAGTTCTCGATCACCCAAATGTTGCATGCTTGAAGCATTA
CTTTTGCTCGACTACTGCGAAGGAGGAGCTATACCTCAACTTGGTTCTTGAATATGTGCCAGAA
ACTGTTCATCGTGTTATTAGACATTACAATAAGATGAGCCAACGCATGCCATTGATTTATGTTA
AACTTTATATGTACCAG

Sequences
Splice junction sequence	TCTTGAATATGTGCCAGAAACTGTTCATCGTGTTATTAGACATTACAATAAGATGAGCCAACGC ATGCCATTGATTTATGTTAAACTTTATATGTACCAGgctaagtgtctagaaactggtgagagag ttgctgtaaagaaagttcttcaagatgcgagatacaagaaccgggagctgcaaacgatgcaagt tctcgatc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 273 Transcript exons: 10824779-10825051 GCTAAGTGTCTAGAAACTGGTGAGAGAGTTGCTGTAAAGAAAGTTCTTCAAGATGCGAGATACA AGAACCGGGAGCTGCAAACGATGCAAGTTCTCGATCACCCAAATGTTGCATGCTTGAAGCATTA CTTTTGCTCGACTACTGCGAAGGAGGAGCTATACCTCAACTTGGTTCTTGAATATGTGCCAGAA ACTGTTCATCGTGTTATTAGACATTACAATAAGATGAGCCAACGCATGCCATTGATTTATGTTA AACTTTATATGTACCAG
Genomic sequence	GCTAAGTGTCTAGAAACTGGTGAGAGAGTTGCTGTAAAGAAAGTTCTTCAAGATGCGAGATACA AGAACCGGGAGCTGCAAACGATGCAAGTTCTCGATCACCCAAATGTTGCATGCTTGAAGCATTA CTTTTGCTCGACTACTGCGAAGGAGGAGCTATACCTCAACTTGGTTCTTGAATATGTGCCAGAA ACTGTTCATCGTGTTATTAGACATTACAATAAGATGAGCCAACGCATGCCATTGATTTATGTTA AACTTTATATGTACCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_010328 						
					
						PMCS
						0.568077808

Conservation Information
Conserved circRNAs	osi_circ_010328
PMCS	0.568077808

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							10824864-10825048(+)
						
						Potential amino acid sequence
						
							MQVLDHPNVACLKHYFCSTTAKEELYLNLVLEYVPETVHRVIRHYNKMSQRMPLIYVKLYMYQA
KCLETGERVAVKKVLQDARYKNRELQTMQVLDHPNVACLKHYFCSTTAKEELYLNLVLEYVPET
VHRVIRHYNKMSQRMPLIYVKLYMYQAKCLETGERVAVKKVLQDARYKNRELQTMQVLDHPNVA
CLKHYFCSTTAKEELYLNLVLEYVPETVHRVIRHYNKMSQRMPLIYVKLYMYQAKCLETGERVA
VKKVLQDARYKNRELQTMQVLDHPNVACLKHYFCSTTAKEELYLNLVLEYVPETVHRVIRHYNK
MSQRMPLIYVKLYMYQ(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	10824864-10825048(+)
Potential amino acid sequence	MQVLDHPNVACLKHYFCSTTAKEELYLNLVLEYVPETVHRVIRHYNKMSQRMPLIYVKLYMYQA KCLETGERVAVKKVLQDARYKNRELQTMQVLDHPNVACLKHYFCSTTAKEELYLNLVLEYVPET VHRVIRHYNKMSQRMPLIYVKLYMYQAKCLETGERVAVKKVLQDARYKNRELQTMQVLDHPNVA CLKHYFCSTTAKEELYLNLVLEYVPETVHRVIRHYNKMSQRMPLIYVKLYMYQAKCLETGERVA VKKVLQDARYKNRELQTMQVLDHPNVACLKHYFCSTTAKEELYLNLVLEYVPETVHRVIRHYNK MSQRMPLIYVKLYMYQ(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os01g0296100_circ_g.4

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