Plant Circular RNA Database

Detail information of Os03g0227300_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os03g0227300_circ_g.2
					
						ID in PlantcircBase
						osa_circ_018635
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 6702302-6705166  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os03g0227300						
					
						Parent gene annotation
						
							5'-3' exonuclease domain containing protein. (Os03t0227300-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os03g0227300_circ_g.3 Os03g0227300_circ_g.4 						
					
						Support reads
						1
					
						Tissues
						root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0227300-01:7

General Information
CircRNA Name	Os03g0227300_circ_g.2
ID in PlantcircBase	osa_circ_018635
Alias	NA
Organism	Oryza sativa
Position	chr3: 6702302-6705166 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os03g0227300
Parent gene annotation	5'-3' exonuclease domain containing protein. (Os03t0227300-01)
Parent gene strand	+
Alternative splicing	Os03g0227300_circ_g.3 Os03g0227300_circ_g.4
Support reads	1
Tissues	root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0227300-01:7

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CCAAAAGTTATAACTTTGTCATTTTATTGGTACTCACATAGGTTCCTTGGAGAATCTGTTGAAA
TCTGTGGATGAAGTTGAGGAAGAACGAATTAAACAGgagtcccttatggtcattatactgccat
gccatccaaagaatgtcacatggcaaaaggtaagagtgatatactgaagtaacttgataggatc
atgggatt

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							GAGTCCCTTATGGTCATTATACTGCCATGCCATCCAAAGAATGTCACATGGCAAAAGGTAAGAG
TGATATACTGAAGTAACTTGATAGGATCATGGGATTCATGGGAGAATGTAACCGTTGGATTCAT
CTAAGATTTTAGGCATAACCACATCTTTATGTGATGGATGTCCTGGATTTATGTCGCACTTTAT
GTTAGTCCATTACTTAGCCAAGTATCAAAGCAGTAGGAGTGATTTAACATGACCTTCCAAATGC
CTGCCTTTCATTTCTTTTATGACTTTTAGGGAGATACTGCTTATTTCTGATCCTATTTCAAATC
ATCCTTTTCTTTTCCCCTAGTAGAATAATACGATGATATTTCATTTTATATTTGGGAATGATAG
TTTAATACTATATATGGTTTTTACACTTGTGTACCCTGGCTCATCTGTATTTGCTTTGGGGCTT
CTGGAGACTGGACTTGTTTCTAGCCTGCTGGGCTGGAACAATCCATCTTACTTATTTCCCATTT
TACTTTCCTTTTCTATTACTCTTATATAGTTGTACACTGAAGAACAATTAAAATTTCAATTGGA
AGTAATGTCTTGCAATGTTTTCAATCCTATAAAAGCATGAAACTGTCTTTTTTTTTTCAGGAAT
GACTTTTCGTCACATGTTATACCCTTCCTACAAGAGCAACCGCATCCCGACACCAGACACCATT
GTCCAGGGCATGCAATACTTGAAGGCATCTATTAAAGCGATGTCAATCAAAGTGATTGAGGTAA
AAGATATATTAGCAAGTGTATGATTGGCATTATATTCTTTATTATATATTTTCATTTGAGTTCT
TAAATGATGGTTGTAGGTTCCTGGTGTCGAAGCTGATGATGTTATTGGCACACTAGCTGTCAGC
AGTGTATCTGCAGGCTACAAGGTAATAAAACTTTCTACTGTGATTATTTGTGAACATTTTATTC
ATGAATTGTTCTTCAATATTCTCAATGGATATTTCATAAGTATCTCTCTTCATGAATCGTTCTC
CAGTGCCGTCTTTAATCTTTCACATCATTTACAAAAGTTAGTACTTATAAACTGCTGAGGTGTT
ATAGATTCACTGAAACTGTTTTTATTTGTTTTGAAATGATTGATATTTTGTAAAAGTTGATTCA
TAACTAACTTTTCCAATTGCACATGACAAAACTGAATGTCTGGAATTAAGTCATAGGTATGTTG
AATTTTGTAACAAAAATGCAAGAAATACAGTGTAACTAAAATGTGATGCCGTTGCAGTGTGTTG
GATATGATATCTTATTGTGTACTCTGGTCCTTATTTGAAGGTACGGATTGTCTCCCCTGATAAA
GACTTCTTCCAAATTCTTTCACCTTCTTTACGTTTGCTGAGGATTGCTCCTCGTGGTTCTGGGT
AAGTGTACTTGGTTTTTTTTGCCTTTCTTGCATGCTTACTGGAATACTCTAATAGCTAATCGTT
TTTATTTGGACATGGAGGATGGTTTCATTTGGAGTTGAAGACTTTGTCAAGAGATATGGAGCAC
TGAAACCATCACAGTTTGTTGACGTTGTTGCGCTTTCTGGAGATAAAGCCGACAATATACCAGG
TACCCTTTATTTCTATTGTGCCTTATGCTACACATCTGTTCTGACATCCATTCTTCTTTCAGAC
TAATTTTTGTTGGTAGCTATGAAAACTATGAGAGAAAGGATACTAAACTTTGGTCTGCATTGGG
TTGTATTTATTTATTTATTTCTCGCAATAAATTCTTGTGCTTGGCTACCTTTGTTTTTCTTGTC
CAATATCTTTCATTTCAGCTCTAAAACAGAAAAATGGACCACAGTTGCACACGATTTTGATGTG
TCAAAGTATTTTTAAGTTGATAATGTGATAGAAAATAAAGTTCATACACTGTATATAATGTGCT
TGCCGAAGAAAATGGTGGTTGCATGATCTATAGACATAGTGATTTCATTTTGGTTCTTCTACCT
ATTTATTTATTATTTATTATGTGCTGTAATCTTACTGTTTGATAAAAACCTTAAATCAACTCAT
CAAATATGTTATGGACCCTATGAAGTCTACTGTCCCCCATCTTTTTAAGGGGAAAAACATGATT
CTCATGTCTGAAAATTATTTCATGTTATGATATATGTTTGTACGTTACCAACACTTCACCAGTG
CAATGCAGATTCGTAAGCTAATTTTCTATATATGGTAGTCTATCGACCAAGTTGTTTATGATGT
GACCGGAACCATGTTCTTTTTCAGGAGTTGAAGGGATTGGAGATATTAATGCGGTAAAACTGAT
AACTAAGTTTGGTATGTTCTTCTCTTTAAAGTTTTTTTTTAGATTGTCGCCTTGGTGCGAACCA
CTCGAGCTAAACACATTCTGTTCTAATAAAGGACTGCTTGTATACACTCAAACTACTCAAGATT
GAAAAGTTCATGATTTCCCTGTGATCAATCTACCTGTTTAGTCTTGGTTATTAAGTTCTACTCC
CCTAAAAAATATAAGTAGTTTTTAGCTTTCTCAAGTCTTTCCTAAATGTAAGTCATCCTAAACT
TCTAGCCACCTTTTACTCCTCCCTTTCTTCCGTGAATAATTCACATTATTCAATGTGGTATAAG
TAAAGGGTAACAAGGTCGTTTGATGTACTTTATTATCCCTGTACATAAATCTAGAATAACTTAT
ATTTGGAATCCAAGTATTCTATGTTTTGAGTTTGGAAATAGTGGGCATATTAGCTGTCCTGATA
ATCCTGCAATTCTCCAAAAGTTATAACTTTGTCATTTTATTGGTACTCACATAGGTTCCTTGGA
GAATCTGTTGAAATCTGTGGATGAAGTTGAGGAAGAACGAATTAAACAG
						
					

			
		

Sequences
Splice junction sequence	CCAAAAGTTATAACTTTGTCATTTTATTGGTACTCACATAGGTTCCTTGGAGAATCTGTTGAAA TCTGTGGATGAAGTTGAGGAAGAACGAATTAAACAGgagtcccttatggtcattatactgccat gccatccaaagaatgtcacatggcaaaaggtaagagtgatatactgaagtaacttgataggatc atgggatt
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GAGTCCCTTATGGTCATTATACTGCCATGCCATCCAAAGAATGTCACATGGCAAAAGGTAAGAG TGATATACTGAAGTAACTTGATAGGATCATGGGATTCATGGGAGAATGTAACCGTTGGATTCAT CTAAGATTTTAGGCATAACCACATCTTTATGTGATGGATGTCCTGGATTTATGTCGCACTTTAT GTTAGTCCATTACTTAGCCAAGTATCAAAGCAGTAGGAGTGATTTAACATGACCTTCCAAATGC CTGCCTTTCATTTCTTTTATGACTTTTAGGGAGATACTGCTTATTTCTGATCCTATTTCAAATC ATCCTTTTCTTTTCCCCTAGTAGAATAATACGATGATATTTCATTTTATATTTGGGAATGATAG TTTAATACTATATATGGTTTTTACACTTGTGTACCCTGGCTCATCTGTATTTGCTTTGGGGCTT CTGGAGACTGGACTTGTTTCTAGCCTGCTGGGCTGGAACAATCCATCTTACTTATTTCCCATTT TACTTTCCTTTTCTATTACTCTTATATAGTTGTACACTGAAGAACAATTAAAATTTCAATTGGA AGTAATGTCTTGCAATGTTTTCAATCCTATAAAAGCATGAAACTGTCTTTTTTTTTTCAGGAAT GACTTTTCGTCACATGTTATACCCTTCCTACAAGAGCAACCGCATCCCGACACCAGACACCATT GTCCAGGGCATGCAATACTTGAAGGCATCTATTAAAGCGATGTCAATCAAAGTGATTGAGGTAA AAGATATATTAGCAAGTGTATGATTGGCATTATATTCTTTATTATATATTTTCATTTGAGTTCT TAAATGATGGTTGTAGGTTCCTGGTGTCGAAGCTGATGATGTTATTGGCACACTAGCTGTCAGC AGTGTATCTGCAGGCTACAAGGTAATAAAACTTTCTACTGTGATTATTTGTGAACATTTTATTC ATGAATTGTTCTTCAATATTCTCAATGGATATTTCATAAGTATCTCTCTTCATGAATCGTTCTC CAGTGCCGTCTTTAATCTTTCACATCATTTACAAAAGTTAGTACTTATAAACTGCTGAGGTGTT ATAGATTCACTGAAACTGTTTTTATTTGTTTTGAAATGATTGATATTTTGTAAAAGTTGATTCA TAACTAACTTTTCCAATTGCACATGACAAAACTGAATGTCTGGAATTAAGTCATAGGTATGTTG AATTTTGTAACAAAAATGCAAGAAATACAGTGTAACTAAAATGTGATGCCGTTGCAGTGTGTTG GATATGATATCTTATTGTGTACTCTGGTCCTTATTTGAAGGTACGGATTGTCTCCCCTGATAAA GACTTCTTCCAAATTCTTTCACCTTCTTTACGTTTGCTGAGGATTGCTCCTCGTGGTTCTGGGT AAGTGTACTTGGTTTTTTTTGCCTTTCTTGCATGCTTACTGGAATACTCTAATAGCTAATCGTT TTTATTTGGACATGGAGGATGGTTTCATTTGGAGTTGAAGACTTTGTCAAGAGATATGGAGCAC TGAAACCATCACAGTTTGTTGACGTTGTTGCGCTTTCTGGAGATAAAGCCGACAATATACCAGG TACCCTTTATTTCTATTGTGCCTTATGCTACACATCTGTTCTGACATCCATTCTTCTTTCAGAC TAATTTTTGTTGGTAGCTATGAAAACTATGAGAGAAAGGATACTAAACTTTGGTCTGCATTGGG TTGTATTTATTTATTTATTTCTCGCAATAAATTCTTGTGCTTGGCTACCTTTGTTTTTCTTGTC CAATATCTTTCATTTCAGCTCTAAAACAGAAAAATGGACCACAGTTGCACACGATTTTGATGTG TCAAAGTATTTTTAAGTTGATAATGTGATAGAAAATAAAGTTCATACACTGTATATAATGTGCT TGCCGAAGAAAATGGTGGTTGCATGATCTATAGACATAGTGATTTCATTTTGGTTCTTCTACCT ATTTATTTATTATTTATTATGTGCTGTAATCTTACTGTTTGATAAAAACCTTAAATCAACTCAT CAAATATGTTATGGACCCTATGAAGTCTACTGTCCCCCATCTTTTTAAGGGGAAAAACATGATT CTCATGTCTGAAAATTATTTCATGTTATGATATATGTTTGTACGTTACCAACACTTCACCAGTG CAATGCAGATTCGTAAGCTAATTTTCTATATATGGTAGTCTATCGACCAAGTTGTTTATGATGT GACCGGAACCATGTTCTTTTTCAGGAGTTGAAGGGATTGGAGATATTAATGCGGTAAAACTGAT AACTAAGTTTGGTATGTTCTTCTCTTTAAAGTTTTTTTTTAGATTGTCGCCTTGGTGCGAACCA CTCGAGCTAAACACATTCTGTTCTAATAAAGGACTGCTTGTATACACTCAAACTACTCAAGATT GAAAAGTTCATGATTTCCCTGTGATCAATCTACCTGTTTAGTCTTGGTTATTAAGTTCTACTCC CCTAAAAAATATAAGTAGTTTTTAGCTTTCTCAAGTCTTTCCTAAATGTAAGTCATCCTAAACT TCTAGCCACCTTTTACTCCTCCCTTTCTTCCGTGAATAATTCACATTATTCAATGTGGTATAAG TAAAGGGTAACAAGGTCGTTTGATGTACTTTATTATCCCTGTACATAAATCTAGAATAACTTAT ATTTGGAATCCAAGTATTCTATGTTTTGAGTTTGGAAATAGTGGGCATATTAGCTGTCCTGATA ATCCTGCAATTCTCCAAAAGTTATAACTTTGTCATTTTATTGGTACTCACATAGGTTCCTTGGA GAATCTGTTGAAATCTGTGGATGAAGTTGAGGAAGAACGAATTAAACAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.106379828

Conservation Information
Conserved circRNAs	NA
PMCS	0.106379828

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							6705139-6703037(+)
6702329-6702940(+)
6702320-6703214(-)
6703035-6703848(-)
						
						Potential amino acid sequence
						
							MKLRKNELNRSPLWSLYCHAIQRMSHGKRNDFSSHVIPFLQEQPHPDTRHHCPGHAILEGIY*(
+)
MPSKECHMAKGMTFRHMLYPSYKSNRIPTPDTIVQGMQYLKASIKAMSIKVIEVPGVEADDVIG
TLAVSSVSAGYKVRIVSPDKDFFQILSPSLRLLRIAPRGSGMVSFGVEDFVKRYGALKPSQFVD
VVALSGDKADNIPGVEGIGDINAVKLITKFGSLENLLKSVDEVEEERIKQESLMVIILPCHPKN
VTWQKE*(+)
MTIRDSCLIRSSSTSSTDFNRFSKEPNLVISFTALISPIPSTPGILSALSPESATTSTNCDGFS
APYLLTKSSTPNETIPEPRGAILSKRKEGERIWKKSLSGETIRTL*(-)
MPSSIACPGQWCLVSGCGCSCRKGITCDEKSFLLPCDILWMAWQYNDHKGLLFNSFFLNFIHRF
QQILQGTKLSYQFYRINISNPFNSWYIVGFISRKRNNVNKL*(-)
						
						Sponge-miRNAs
						
							osa-miR2275d 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	6705139-6703037(+) 6702329-6702940(+) 6702320-6703214(-) 6703035-6703848(-)
Potential amino acid sequence	MKLRKNELNRSPLWSLYCHAIQRMSHGKRNDFSSHVIPFLQEQPHPDTRHHCPGHAILEGIY( +) MPSKECHMAKGMTFRHMLYPSYKSNRIPTPDTIVQGMQYLKASIKAMSIKVIEVPGVEADDVIG TLAVSSVSAGYKVRIVSPDKDFFQILSPSLRLLRIAPRGSGMVSFGVEDFVKRYGALKPSQFVD VVALSGDKADNIPGVEGIGDINAVKLITKFGSLENLLKSVDEVEEERIKQESLMVIILPCHPKN VTWQKE(+) MTIRDSCLIRSSSTSSTDFNRFSKEPNLVISFTALISPIPSTPGILSALSPESATTSTNCDGFS APYLLTKSSTPNETIPEPRGAILSKRKEGERIWKKSLSGETIRTL(-) MPSSIACPGQWCLVSGCGCSCRKGITCDEKSFLLPCDILWMAWQYNDHKGLLFNSFFLNFIHRF QQILQGTKLSYQFYRINISNPFNSWYIVGFISRKRNNVNKL(-)
Sponge-miRNAs	osa-miR2275d
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os03g0227300_circ_g.2

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