Plant Circular RNA Database

Detail information of Os03g0336300_circ_g.5

			
						 General Information
					
						CircRNA Name
						Os03g0336300_circ_g.5
					
						ID in PlantcircBase
						osa_circ_019683
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr3: 12465847-12466419  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os03g0336300						
					
						Parent gene annotation
						
							Peptidase M16, core domain containing protein. (Os03t0336300-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os03g0336300_circ_g.6 Os03g0336300_circ_g.7 						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os03t0336300-01:2

General Information
CircRNA Name	Os03g0336300_circ_g.5
ID in PlantcircBase	osa_circ_019683
Alias	NA
Organism	Oryza sativa
Position	chr3: 12465847-12466419 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	Os03g0336300
Parent gene annotation	Peptidase M16, core domain containing protein. (Os03t0336300-01)
Parent gene strand	+
Alternative splicing	Os03g0336300_circ_g.6 Os03g0336300_circ_g.7
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os03t0336300-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GGCAAGGCATGAAGAAAGAGTTCTTTGCATTCCTGATGATACAAATTTTGTTAGAAGTGTCCGT
GTGAAGAATGAACTTGAAGAGAATTCTGTGGTTGAGgtcatcaaggaggatttggaaagagctt
acaaaaacacgaatatgaagcctatgagccattcaacctacctgaggttacaagtcctacgtga
aatattct

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 387
Transcript exons: 12465847-12466029,12466216-12466419
GTCATCAAGGAGGATTTGGAAAGAGCTTACAAAAACACGAATATGAAGCCTATGAGCCATTCAA
CCTACCTGAGGTTACAAGTCCTACGTGAAATATTCTGGGATGTCGATGAAAAGTTGGAAGTTCT
TATGAAGTTAACCTTTTCTGATCTGGTGGCATATGTGCCAAAGCTTCTTTCGCAGTTGCACATC
GAGGGCCTCTGTCATGGTAACCTATCAGAAGATGAAGCAATGAATATATCAAAAATATTCCAAA
ACACATTGTCAGCTCAGACACTGCCTGATGAGGCAAGGCATGAAGAAAGAGTTCTTTGCATTCC
TGATGATACAAATTTTGTTAGAAGTGTCCGTGTGAAGAATGAACTTGAAGAGAATTCTGTGGTT
GAG
						
						Genomic sequence
						
							GTCATCAAGGAGGATTTGGAAAGAGCTTACAAAAACACGAATATGAAGCCTATGAGCCATTCAA
CCTACCTGAGGTTACAAGTCCTACGTGAAATATTCTGGGATGTCGATGAAAAGTTGGAAGTTCT
TATGAAGTTAACCTTTTCTGATCTGGTGGCATATGTGCCAAAGCTTCTTTCGCAGGTATCCATT
ACCATGATGATCTTTTATGCGATTTTTTTCTCCACTTAACCACCTTTTCCTACATAATAGCAAA
TGATTTTCAAATATGGAAATGTTTAATGTTTCTGAGGAACAAAAAAATGGATGTTCAACGTACC
ACATGATTTTCTATATACTTCATATGATTATCTGTTTGCACACATGCAGTTGCACATCGAGGGC
CTCTGTCATGGTAACCTATCAGAAGATGAAGCAATGAATATATCAAAAATATTCCAAAACACAT
TGTCAGCTCAGACACTGCCTGATGAGGCAAGGCATGAAGAAAGAGTTCTTTGCATTCCTGATGA
TACAAATTTTGTTAGAAGTGTCCGTGTGAAGAATGAACTTGAAGAGAATTCTGTGGTTGAG

Sequences
Splice junction sequence	GGCAAGGCATGAAGAAAGAGTTCTTTGCATTCCTGATGATACAAATTTTGTTAGAAGTGTCCGT GTGAAGAATGAACTTGAAGAGAATTCTGTGGTTGAGgtcatcaaggaggatttggaaagagctt acaaaaacacgaatatgaagcctatgagccattcaacctacctgaggttacaagtcctacgtga aatattct
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 387 Transcript exons: 12465847-12466029,12466216-12466419 GTCATCAAGGAGGATTTGGAAAGAGCTTACAAAAACACGAATATGAAGCCTATGAGCCATTCAA CCTACCTGAGGTTACAAGTCCTACGTGAAATATTCTGGGATGTCGATGAAAAGTTGGAAGTTCT TATGAAGTTAACCTTTTCTGATCTGGTGGCATATGTGCCAAAGCTTCTTTCGCAGTTGCACATC GAGGGCCTCTGTCATGGTAACCTATCAGAAGATGAAGCAATGAATATATCAAAAATATTCCAAA ACACATTGTCAGCTCAGACACTGCCTGATGAGGCAAGGCATGAAGAAAGAGTTCTTTGCATTCC TGATGATACAAATTTTGTTAGAAGTGTCCGTGTGAAGAATGAACTTGAAGAGAATTCTGTGGTT GAG
Genomic sequence	GTCATCAAGGAGGATTTGGAAAGAGCTTACAAAAACACGAATATGAAGCCTATGAGCCATTCAA CCTACCTGAGGTTACAAGTCCTACGTGAAATATTCTGGGATGTCGATGAAAAGTTGGAAGTTCT TATGAAGTTAACCTTTTCTGATCTGGTGGCATATGTGCCAAAGCTTCTTTCGCAGGTATCCATT ACCATGATGATCTTTTATGCGATTTTTTTCTCCACTTAACCACCTTTTCCTACATAATAGCAAA TGATTTTCAAATATGGAAATGTTTAATGTTTCTGAGGAACAAAAAAATGGATGTTCAACGTACC ACATGATTTTCTATATACTTCATATGATTATCTGTTTGCACACATGCAGTTGCACATCGAGGGC CTCTGTCATGGTAACCTATCAGAAGATGAAGCAATGAATATATCAAAAATATTCCAAAACACAT TGTCAGCTCAGACACTGCCTGATGAGGCAAGGCATGAAGAAAGAGTTCTTTGCATTCCTGATGA TACAAATTTTGTTAGAAGTGTCCGTGTGAAGAATGAACTTGAAGAGAATTCTGTGGTTGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.178012827

Conservation Information
Conserved circRNAs	NA
PMCS	0.178012827

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							12465890-12466416(+)
12466392-12465889(+)
12465908-12466299(-)
12465853-12466304(-)
						
						Potential amino acid sequence
						
							MKPMSHSTYLRLQVLREIFWDVDEKLEVLMKLTFSDLVAYVPKLLSQLHIEGLCHGNLSEDEAM
NISKIFQNTLSAQTLPDEARHEERVLCIPDDTNFVRSVRVKNELEENSVVEVIKEDLERAYKNT
NMKPMSHSTYLRLQVLREIFWDVDEKLEVLMKLTFSDLVAYVPKLLSQLHIEGLCHGNLSEDEA
MNISKIFQNTLSAQTLPDEARHEERVLCIPDDTNFVRSVRVKNELEENSVVEVIKEDLERAYKN
TNMKPMSHSTYLRLQVLREIFWDVDEKLEVLMKLTFSDLVAYVPKLLSQLHIEGLCHGNLSEDE
AMNISKIFQNTLSAQTLPDEARHEERVLCIPDDTNFVRSVRVKNELEENSVVEVIKEDLERAYK
NTNMKPMSHSTYLRLQVLREIFWDVDEKLEVLMKLTFSDLVAYVPKLLSQLHIEGLCHGNLSED
EAMNISKIFQNTLSAQTLPDEARHEERVLCIPDDTNFVRSVRVKNELEENSVVE(+)
MNLKRILWLRSSRRIWKELTKTRI*(+)
MAHRLHIRVFVSSFQILLDDLNHRILFKFILHTDTSNKICIIRNAKNSFFMPCLIRQCLS*(-)
MTSTTEFSSSSFFTRTLLTKFVSSGMQRTLSSCLASSGSV*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	12465890-12466416(+) 12466392-12465889(+) 12465908-12466299(-) 12465853-12466304(-)
Potential amino acid sequence	MKPMSHSTYLRLQVLREIFWDVDEKLEVLMKLTFSDLVAYVPKLLSQLHIEGLCHGNLSEDEAM NISKIFQNTLSAQTLPDEARHEERVLCIPDDTNFVRSVRVKNELEENSVVEVIKEDLERAYKNT NMKPMSHSTYLRLQVLREIFWDVDEKLEVLMKLTFSDLVAYVPKLLSQLHIEGLCHGNLSEDEA MNISKIFQNTLSAQTLPDEARHEERVLCIPDDTNFVRSVRVKNELEENSVVEVIKEDLERAYKN TNMKPMSHSTYLRLQVLREIFWDVDEKLEVLMKLTFSDLVAYVPKLLSQLHIEGLCHGNLSEDE AMNISKIFQNTLSAQTLPDEARHEERVLCIPDDTNFVRSVRVKNELEENSVVEVIKEDLERAYK NTNMKPMSHSTYLRLQVLREIFWDVDEKLEVLMKLTFSDLVAYVPKLLSQLHIEGLCHGNLSED EAMNISKIFQNTLSAQTLPDEARHEERVLCIPDDTNFVRSVRVKNELEENSVVE(+) MNLKRILWLRSSRRIWKELTKTRI(+) MAHRLHIRVFVSSFQILLDDLNHRILFKFILHTDTSNKICIIRNAKNSFFMPCLIRQCLS(-) MTSTTEFSSSSFFTRTLLTKFVSSGMQRTLSSCLASSGSV*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os03g0336300_circ_g.5

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