Plant Circular RNA Database

Detail information of POPTR_0008s12860_circ_ag.2

			
						 General Information
					
						CircRNA Name
						POPTR_0008s12860_circ_ag.2
					
						ID in PlantcircBase
						pop_circ_002377
					
						Alias
						Chr08:8504012|8508400
					
						Organism
						
							Populus trichocarpa						
					
						Position
						
							chr8: 8368133-8372525  JBrowse»						
					
						Reference genome
						
							Populus trichocarpa genome v3.0 						
					
						Type  
						
							ag-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							NA						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						NA
					
						Alternative splicing
						
							POPTR_0008s12860_circ_ag.1 POPTR_0008s12860_circ_ag.3 POPTR_0008s12860_circ_ag.4 POPTR_0008s12860_circ_ag.5 POPTR_0008s12860_circ_ag.6 						
					
						Support reads
						NA
					
						Tissues
						stem cambium
					
						Exon boundary  
						
							NA-NA							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GG-AT							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						NA

General Information
CircRNA Name	POPTR_0008s12860_circ_ag.2
ID in PlantcircBase	pop_circ_002377
Alias	Chr08:8504012\|8508400
Organism	Populus trichocarpa
Position	chr8: 8368133-8372525 JBrowse»
Reference genome	Populus trichocarpa genome v3.0
Type	ag-circRNA
Identification method	find_circ
Parent gene	NA
Parent gene annotation	NA
Parent gene strand	NA
Alternative splicing	POPTR_0008s12860_circ_ag.1 POPTR_0008s12860_circ_ag.3 POPTR_0008s12860_circ_ag.4 POPTR_0008s12860_circ_ag.5 POPTR_0008s12860_circ_ag.6
Support reads	NA
Tissues	stem cambium
Exon boundary	NA-NA "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GG-AT The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	NA

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TTCAGTTTTATCTCCTCTGAATAACGTGTTAATTTAAATAAAAACAAGATCCAGCAGATACAAT
CCCATCATGTTGTTCAAATAAAAACATCATTTGGATggtgcgtgatagatgaaaacgaagcgtg
ggatttgttcattgcaatgtctgacacgaaaccctctccttgcaacagtaacagtagtagtagt
agcaactg

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							GGTGCGTGATAGATGAAAACGAAGCGTGGGATTTGTTCATTGCAATGTCTGACACGAAACCCTC
TCCTTGCAACAGTAACAGTAGTAGTAGTAGCAACTGATTTCATTTCAGAATGCCCCTCTGAAGC
GTGGGATTTGTTCATTGCAATGCCTGACACGAAATCCTCCCCTTGCAACAGTAGCAGTAGTAGC
AGCTTACTGATTTCAGAGTGCTCCTCTGGAATCTGACACGGAATCCTCCCCTTCCACATTAGCA
AAGACCCCCCTTTCCAGGACCCAATCCTCTATCATAGCCTGAGAATTCACTGCTTGGTCAGTAG
GCACAAGATGCCCAGCCCCTAACACCACAGCATTGCTAAAACTCCTCCACTTCTGCACATACCC
AGCAAGCACACCATTTACTTTCCACACCTTCCTCTCAGCCATCAAGTACTGCCCTATCCCTTCC
CATTTCATTGTCTTGACCCAAGCCTCTGTTGAAACCACTCCATCTCTCAAATCAAAATGCCCTT
GATACAACAACACGTTACTCTTCTTAACCAAGAATTCCACCATATACTTAACGCTCTTCATTAC
ATCTTCATGCAATGCTTCCCCCACCGTGTCACTACAATCCTCAAAAACTATAGATTCATTTGCC
TTCAATGCAACCTTCACTTCGGCTAGTTGCATCAATTTAGTTACCAATTCTGTTTCGTAAGGCA
CTTTTCTAGTGAAGTCATACAGAGTGGCTAGTCCTGTCATGTTTTGCAACAAATTCAAGACCCT
ACTTCTTGCATCTGTTGCCTCACTCCAATTTCCCATTTTAACCAACTTAACTGCCTCCCTTTGA
GCTTCTTCCAGTTCACCCTTTTGTCTCTCATTGATCAATCCAGAAAAGTAAGCATTCAGGGCAT
GAGTTTTGACTTGTGTCACCGGATCCGTTAACCCATTACCTATAGCAACACCTTTCAGATTCAC
TTGTTTCGCCGCAGGCAGCTTCGTATTCTTCTTCAAAATGTAATACCCAATTGCAGGAACATAC
TTTCCTGCATAACTCTCTCCGGTTATATAAATCGGGCGAGTCTTGAACACTGGGTCTAGTTTAA
TAAACTCAGAGATGGCGGCGAAGAGATGCTCAGCAACAGTGTGTTGATCTCTTGGTATCTCTTC
AGGGCTCGAAGCGATACTAAATCCGGTTCCTATTGGGTTATCGATGAAAATTAGGCCAAAAATA
CGGTTCCAGGAGCCTAAATTAGGCTGAAGAGCAGGGTGGTCGTTGTCTTGCGAATCGACAACGC
GATAAGGGCCAAGTTCAAGGAAGTTTCCAGTCATGGAGGAGCAGCCAGGGCCACCTTGGAGCCA
GATGAGAAGTGGGGTTTGGGAAAGAGGTGAAGTCGGTTTTTGAGCCTCGTAAAACGTGTAGAAA
ATAGCTGAGTTGGTTTTGGGTTTGACAGGGATATAACCTGAATTTGTGGGGAGAGCTTCGTTTG
GGAAAATAGAGGTGGGTGTTGATGATGTAGCTGAAGGATGGTGGAGGAAGAGGAAGAGGAGGAA
GAGGAAGAGGACTGTTGTTGACTCCATTAGCGAAGGAGGACTGAGTTAGCGGAGAATTGTTGAT
CTTTTGTTGTTTAGGTTTGTCTGATCTGGTGGCTTTGATAGTTGTGACCAAAGAATCTTCGGTC
CCGCGTGGAGGGAGAAGTACGACGACAATAATGTCTTGAACAAAGCTGATTAATTGTAAAGAGC
ATCTCCGAGGGAAAGATGCTATTTTGAAGAGTAAAATAGTATTTTGAATTCAATAAATATGGTT
TTTTTTTGAAAAAAAGTTATTTTAAAGAATAATTGTATTAAAATGAAAGAAAGAACTATTTTAT
TTTTTAAATGATTTTGTTTTTTTTTAACATAGTTATATTTAATTGGTTTATTTTTTGTTGGCTT
TAATTTGTTGGTTTTGATTTTTTAAAAAAATATATATAAATAACATTTTTAAAAGAGAAAAAGT
ATTTTAACATTTTATTTAGTTTTTTTTTTTCGGAGCATGTAAAATTATTATTAAAAATTTTTTA
TTTTTATTTTTTATTTAGCACACCCCTTGTACTTGCTCAAGGAGTGATTAGCTTTTGCTTTTAA
AATGCTAGAGATTAACGTTAACACCAAATATATTGGTCTGAGATATCCATGATTTTTTTATGAT
CGTTATTAAATATTTTTTCATAGAATTTTAAGAATGCATATCTGGTATATATTCCACTAATACT
TTTTTTGGAAATAATTTTAAAAGCCAGCTGTATTGCCAGTTATTTCATTAATTGCATTAAATTT
TATTAGTTAACTGGATCAATTATTTATATATTTAACCAATAAGAATATTTTAATAACATGTTTT
TCCTTATAATTGTTTTATGAATTTTAGGCTTATACAACTTTTTTATACATACTATCTTCTATGA
TGTATTTCGCAAATTATTTTAGAGACTTGATCGAAATCAAGTATATAGTTGGAAAACTTATTTG
TAGTCTCTATTTTCTAAGTTTAGTATCCAATTTATCAATATTATACTCCATGTTTTCACATTTT
ACTCTAATTTATTTTCCCTTGTTTTTTCTTTTCTTCTCGGTAGTCAAATCTTCTTTTTTTATTA
GTTGCATCTTTTGTAGCCAGACCTGTATATTTAAGCTTTTTTTTCATTTATATTTTTAATATTT
TTTTCTTTTTAATTTTATCATTAAATGTTATGCTTTTATTGATTTCTTGTATTGCCATTATTTT
TTTATGGTTTAGCATATTTTTAACAATTCTTTATAATTTTTTTAATGTAACATAAATTAATTTT
TTCTTAGTTTACAAATAAGATTTTAATTTTTTTTTAATATCAGGAGGCATTTTTTTTGTGTAGC
CCTACTCAAAAAATAAAAATTCTTTGATTTTGTTCAATCACTTCTCTAAAGTATTTTATTATTG
TTTCATTAAATAAAATTTTAGTTCTAATAAACAAATTTATTACATATAATTGAGTTTATAAACC
ATGTTTAAAGATCAAATATTTATTTTTACATTCATTTTTAAACTTTTTCAATTTGGTTAAATAT
TATATAATTTTTACTTGATTTTCTCATAAAATGATAATACTTTTTTCTAACATGTGCATTCTTG
CATGATATTTTTTATTTAATTTTATCCAATCACTTTTCATATGTGTATTTATTTCTATTATCAT
TTTATTAGGTAAAAAATTAATCCTAATAAATAAAGTTATTAAACACAATTAGTAATATGATCCA
TGTTGCAAGATTAAATATTTTAAACTATATATATCTTTTAATTTTTCTAGTCTAGATTTAAGTT
ATCATCAACAATTTTTTTGAAAACCAAATCTAATTTTATATTTTGAAATTTTAAGTGTATTAAC
TAGTTTTATTGAACACATAAAAAAGAAAATATATTATAATTAACGTTTTGAAACTCACGTTTTG
CTGTTCAAGAAAAAAAAAATCCACCCCCCCTGCTTTTTACATATACACCACGCATCTCGACTAG
GGTTTGGAGGGCAATTCTGTTCAAGCGAAAACGTGGGTAGTGCTAGCGGCGTCTAGGGTTTGAG
TCATCGCATCAATTTCTTTTCGATTTGATATTTTTTCTAAGAGGGCTGAGGCAAAAGGGGGGAG
CAAGATATATCTATCAATTTTAGAATTGGATGTTGCTATGAGGAACGTTGGGGAAATATTTGGT
TATGTGGGGGAAACTTAGATTTGGGATGAAATGAGCGTGTAGATTTCTCAGGTCTCTGTATAAT
GCCAGCTAAGAAGGGAACATGGAATGTAAATGATGAAACCGTGATTGCAAATGGAGTTATAGCC
AGCTACATTCTGGCCTTCAACTTTCAGTTGCTTGATGGAGAAGAGATACGTTCGGCATTGTTAA
ATAAGACCATGGATTGATTGCCCCCTGCCTGGATATTCTTTCCACTTACCAGTGAAAGGGAAAT
GTTGCACAAGCAGTAGGGACTTGGGATGAAATGATTGAGTCATGGAATGATTTGAAGTCTACAA
ATGGAGTTGCAAAATAGTCTTGAGGTTAAAAATATTCGGTGGATGGATCTTGCCTCTGAAATTA
TAAAAATGAAGTGGTTTTCAAAATCAATATGCTACTGAGCGATGCTATAGAGATACCATACCAG
TTTATGGTGAATTTCCCAGGAACCTTGAGATTTCATATGGTAAAAGGTGAAGTGTGTCTGATGA
CAATCTGTCGCGTCTACAGAAGCATCTATGGATGGTAGAATGGATAGCGGAAATTGGATAAGAT
GGTATTTCAGTTTTATCTCCTCTGAATAACGTGTTAATTTAAATAAAAACAAGATCCAGCAGAT
ACAATCCCATCATGTTGTTCAAATAAAAACATCATTTGGAT
						
					

			
		

Sequences
Splice junction sequence	TTCAGTTTTATCTCCTCTGAATAACGTGTTAATTTAAATAAAAACAAGATCCAGCAGATACAAT CCCATCATGTTGTTCAAATAAAAACATCATTTGGATggtgcgtgatagatgaaaacgaagcgtg ggatttgttcattgcaatgtctgacacgaaaccctctccttgcaacagtaacagtagtagtagt agcaactg
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GGTGCGTGATAGATGAAAACGAAGCGTGGGATTTGTTCATTGCAATGTCTGACACGAAACCCTC TCCTTGCAACAGTAACAGTAGTAGTAGTAGCAACTGATTTCATTTCAGAATGCCCCTCTGAAGC GTGGGATTTGTTCATTGCAATGCCTGACACGAAATCCTCCCCTTGCAACAGTAGCAGTAGTAGC AGCTTACTGATTTCAGAGTGCTCCTCTGGAATCTGACACGGAATCCTCCCCTTCCACATTAGCA AAGACCCCCCTTTCCAGGACCCAATCCTCTATCATAGCCTGAGAATTCACTGCTTGGTCAGTAG GCACAAGATGCCCAGCCCCTAACACCACAGCATTGCTAAAACTCCTCCACTTCTGCACATACCC AGCAAGCACACCATTTACTTTCCACACCTTCCTCTCAGCCATCAAGTACTGCCCTATCCCTTCC CATTTCATTGTCTTGACCCAAGCCTCTGTTGAAACCACTCCATCTCTCAAATCAAAATGCCCTT GATACAACAACACGTTACTCTTCTTAACCAAGAATTCCACCATATACTTAACGCTCTTCATTAC ATCTTCATGCAATGCTTCCCCCACCGTGTCACTACAATCCTCAAAAACTATAGATTCATTTGCC TTCAATGCAACCTTCACTTCGGCTAGTTGCATCAATTTAGTTACCAATTCTGTTTCGTAAGGCA CTTTTCTAGTGAAGTCATACAGAGTGGCTAGTCCTGTCATGTTTTGCAACAAATTCAAGACCCT ACTTCTTGCATCTGTTGCCTCACTCCAATTTCCCATTTTAACCAACTTAACTGCCTCCCTTTGA GCTTCTTCCAGTTCACCCTTTTGTCTCTCATTGATCAATCCAGAAAAGTAAGCATTCAGGGCAT GAGTTTTGACTTGTGTCACCGGATCCGTTAACCCATTACCTATAGCAACACCTTTCAGATTCAC TTGTTTCGCCGCAGGCAGCTTCGTATTCTTCTTCAAAATGTAATACCCAATTGCAGGAACATAC TTTCCTGCATAACTCTCTCCGGTTATATAAATCGGGCGAGTCTTGAACACTGGGTCTAGTTTAA TAAACTCAGAGATGGCGGCGAAGAGATGCTCAGCAACAGTGTGTTGATCTCTTGGTATCTCTTC AGGGCTCGAAGCGATACTAAATCCGGTTCCTATTGGGTTATCGATGAAAATTAGGCCAAAAATA CGGTTCCAGGAGCCTAAATTAGGCTGAAGAGCAGGGTGGTCGTTGTCTTGCGAATCGACAACGC GATAAGGGCCAAGTTCAAGGAAGTTTCCAGTCATGGAGGAGCAGCCAGGGCCACCTTGGAGCCA GATGAGAAGTGGGGTTTGGGAAAGAGGTGAAGTCGGTTTTTGAGCCTCGTAAAACGTGTAGAAA ATAGCTGAGTTGGTTTTGGGTTTGACAGGGATATAACCTGAATTTGTGGGGAGAGCTTCGTTTG GGAAAATAGAGGTGGGTGTTGATGATGTAGCTGAAGGATGGTGGAGGAAGAGGAAGAGGAGGAA GAGGAAGAGGACTGTTGTTGACTCCATTAGCGAAGGAGGACTGAGTTAGCGGAGAATTGTTGAT CTTTTGTTGTTTAGGTTTGTCTGATCTGGTGGCTTTGATAGTTGTGACCAAAGAATCTTCGGTC CCGCGTGGAGGGAGAAGTACGACGACAATAATGTCTTGAACAAAGCTGATTAATTGTAAAGAGC ATCTCCGAGGGAAAGATGCTATTTTGAAGAGTAAAATAGTATTTTGAATTCAATAAATATGGTT TTTTTTTGAAAAAAAGTTATTTTAAAGAATAATTGTATTAAAATGAAAGAAAGAACTATTTTAT TTTTTAAATGATTTTGTTTTTTTTTAACATAGTTATATTTAATTGGTTTATTTTTTGTTGGCTT TAATTTGTTGGTTTTGATTTTTTAAAAAAATATATATAAATAACATTTTTAAAAGAGAAAAAGT ATTTTAACATTTTATTTAGTTTTTTTTTTTCGGAGCATGTAAAATTATTATTAAAAATTTTTTA TTTTTATTTTTTATTTAGCACACCCCTTGTACTTGCTCAAGGAGTGATTAGCTTTTGCTTTTAA AATGCTAGAGATTAACGTTAACACCAAATATATTGGTCTGAGATATCCATGATTTTTTTATGAT CGTTATTAAATATTTTTTCATAGAATTTTAAGAATGCATATCTGGTATATATTCCACTAATACT TTTTTTGGAAATAATTTTAAAAGCCAGCTGTATTGCCAGTTATTTCATTAATTGCATTAAATTT TATTAGTTAACTGGATCAATTATTTATATATTTAACCAATAAGAATATTTTAATAACATGTTTT TCCTTATAATTGTTTTATGAATTTTAGGCTTATACAACTTTTTTATACATACTATCTTCTATGA TGTATTTCGCAAATTATTTTAGAGACTTGATCGAAATCAAGTATATAGTTGGAAAACTTATTTG TAGTCTCTATTTTCTAAGTTTAGTATCCAATTTATCAATATTATACTCCATGTTTTCACATTTT ACTCTAATTTATTTTCCCTTGTTTTTTCTTTTCTTCTCGGTAGTCAAATCTTCTTTTTTTATTA GTTGCATCTTTTGTAGCCAGACCTGTATATTTAAGCTTTTTTTTCATTTATATTTTTAATATTT TTTTCTTTTTAATTTTATCATTAAATGTTATGCTTTTATTGATTTCTTGTATTGCCATTATTTT TTTATGGTTTAGCATATTTTTAACAATTCTTTATAATTTTTTTAATGTAACATAAATTAATTTT TTCTTAGTTTACAAATAAGATTTTAATTTTTTTTTAATATCAGGAGGCATTTTTTTTGTGTAGC CCTACTCAAAAAATAAAAATTCTTTGATTTTGTTCAATCACTTCTCTAAAGTATTTTATTATTG TTTCATTAAATAAAATTTTAGTTCTAATAAACAAATTTATTACATATAATTGAGTTTATAAACC ATGTTTAAAGATCAAATATTTATTTTTACATTCATTTTTAAACTTTTTCAATTTGGTTAAATAT TATATAATTTTTACTTGATTTTCTCATAAAATGATAATACTTTTTTCTAACATGTGCATTCTTG CATGATATTTTTTATTTAATTTTATCCAATCACTTTTCATATGTGTATTTATTTCTATTATCAT TTTATTAGGTAAAAAATTAATCCTAATAAATAAAGTTATTAAACACAATTAGTAATATGATCCA TGTTGCAAGATTAAATATTTTAAACTATATATATCTTTTAATTTTTCTAGTCTAGATTTAAGTT ATCATCAACAATTTTTTTGAAAACCAAATCTAATTTTATATTTTGAAATTTTAAGTGTATTAAC TAGTTTTATTGAACACATAAAAAAGAAAATATATTATAATTAACGTTTTGAAACTCACGTTTTG CTGTTCAAGAAAAAAAAAATCCACCCCCCCTGCTTTTTACATATACACCACGCATCTCGACTAG GGTTTGGAGGGCAATTCTGTTCAAGCGAAAACGTGGGTAGTGCTAGCGGCGTCTAGGGTTTGAG TCATCGCATCAATTTCTTTTCGATTTGATATTTTTTCTAAGAGGGCTGAGGCAAAAGGGGGGAG CAAGATATATCTATCAATTTTAGAATTGGATGTTGCTATGAGGAACGTTGGGGAAATATTTGGT TATGTGGGGGAAACTTAGATTTGGGATGAAATGAGCGTGTAGATTTCTCAGGTCTCTGTATAAT GCCAGCTAAGAAGGGAACATGGAATGTAAATGATGAAACCGTGATTGCAAATGGAGTTATAGCC AGCTACATTCTGGCCTTCAACTTTCAGTTGCTTGATGGAGAAGAGATACGTTCGGCATTGTTAA ATAAGACCATGGATTGATTGCCCCCTGCCTGGATATTCTTTCCACTTACCAGTGAAAGGGAAAT GTTGCACAAGCAGTAGGGACTTGGGATGAAATGATTGAGTCATGGAATGATTTGAAGTCTACAA ATGGAGTTGCAAAATAGTCTTGAGGTTAAAAATATTCGGTGGATGGATCTTGCCTCTGAAATTA TAAAAATGAAGTGGTTTTCAAAATCAATATGCTACTGAGCGATGCTATAGAGATACCATACCAG TTTATGGTGAATTTCCCAGGAACCTTGAGATTTCATATGGTAAAAGGTGAAGTGTGTCTGATGA CAATCTGTCGCGTCTACAGAAGCATCTATGGATGGTAGAATGGATAGCGGAAATTGGATAAGAT GGTATTTCAGTTTTATCTCCTCTGAATAACGTGTTAATTTAAATAAAAACAAGATCCAGCAGAT ACAATCCCATCATGTTGTTCAAATAAAAACATCATTTGGAT

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.106235073

Conservation Information
Conserved circRNAs	NA
PMCS	0.106235073

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zheng et al., 2020

circRNA

Detail information of POPTR_0008s12860_circ_ag.2

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