Plant Circular RNA Database

Detail information of Cotton_A_33343_circ_g.1

			
						 General Information
					
						CircRNA Name
						Cotton_A_33343_circ_g.1
					
						ID in PlantcircBase
						gar_circ_000478
					
						Alias
						CA_chr2_BGI-A2_v1.0:18669221|18671279
					
						Organism
						
							Gossypium arboreum						
					
						Position
						
							chrCA_chr2_BGI-A2_v1.0: 18669221-18671279  JBrowse»						
					
						Reference genome
						
							BGI-A2_v1.0						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Cotton_A_33343						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						2
					
						Tissues
						ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Cotton_A_33343:5

General Information
CircRNA Name	Cotton_A_33343_circ_g.1
ID in PlantcircBase	gar_circ_000478
Alias	CA_chr2_BGI-A2_v1.0:18669221\|18671279
Organism	Gossypium arboreum
Position	chrCA_chr2_BGI-A2_v1.0: 18669221-18671279 JBrowse»
Reference genome	BGI-A2_v1.0
Type	e-circRNA
Identification method	CIRI
Parent gene	Cotton_A_33343
Parent gene annotation	NA
Parent gene strand	-
Alternative splicing	NA
Support reads	2
Tissues	ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Cotton_A_33343:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							CAGATAAATAAACAAATTAAAAATAAAGACTACCAAAAAGGAAAACAGAGAACGATATACTAAC
CTGATGATCGGGAACTTGCCTGATGTTACTGACATCtttatcacgatgggctcatatgcagtga
tcagaacatctgttcctacttccttaagacgtatgtttgcaagatagaccttcatggatattca
tcaagaaa

									
								

					

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							NA						
					

						Genomic sequence
						
							TTTATCACGATGGGCTCATATGCAGTGATCAGAACATCTGTTCCTACTTCCTTAAGACGTATGT
TTGCAAGATAGACCTTCATGGATATTCATCAAGAAACCATTTTTCAGTTTAGAACTGAATAAAA
ATTGCGAAAGCTGAAAACACAAGAAATGTTACCTTGATAATATTCTGAGCTTCTCTTCCTTGCC
GTCCCTTAGAAACAGCCTATGCATAGAAGGGATTAAAAGATATGAGAGACAGAAGGGAAGCAAT
GAAAGTCAAGAACTAATATCCAACCACCAATCTAACTGGTGGTATAGTAACCAGGCATTAAGAC
ACATATTACTGTCCCAGACAGTCTACAATGAACCATTTGACAGTCTTGGCAATACAGACTAACA
ACAATAAAGCAATACCTTTTGCTTTGCTCTAACCAGGAGAAATTGCACCTGGTATTGGGGCGGA
TCAATAATAGCAAAAAGTTCATGACATTGAAGATACAAGACATGCACCCAAAATTTAAGATTAA
TTGTTTCAGAACTTCCCAACACAAGTTCAGACCATTGCCAACAGTTTTTTTACCTTGCTAATAT
CATCTAATCTTACAATTTCAGTCTGTCAATAATCGAAACATGCTATAAAGAAAATATTATTCGT
AAGTCTCAGATATGCATCCCCTCTGTATTAACATAAAAATATGCTAGAGGCAATCAACCTAAAT
TCTAGCTTTTACACCCGCACATGCATATTCATAATAGCACACACAATGAAGGAACACATTATCA
TTTAAGTCAATGAACAGCACATAGAGAGTATCCAATCTGACATAGTCTGGTAGAATTTAAAGGT
TACTGTGCCAAGCATGTAGATTAAGATGATAGAATGTTAAGAGTAAGAAATATTGAGACTCGAG
GAAGAGAATAAAGTTTGGCAAGGCAGATATTTGTGGACAACAGAAAAATGAAAGTTGAAACTAG
TAACAATGGAACTCTAAGCAAATGTCTGCTAAATGACTCATACCATTTGGCCAACTGCAGTTGT
AACAATGGCTGGAATGTTTTTGTAACACAATCCAGGAGCCTCAACCACTGTTGACTGCTCAAGC
AGCTGAAACATATAGCAAGAAAGAAAATGTTAGGCTAAACCATATCAAGTCTCAAACAGTTGCC
AAGCAAACAAAAGAACATGCAGAAACAATGTACCGAATGTCTTTAACATCAATTGGACCTAAGG
AAATATGAAAGACAACCAGACCAATGCATAAAACACAGTATCCTAATTGTCAAAGAACAAGTAG
AGCTTATAGTTCTTATTTCATTAACAATGATATAGCCAATTAGCCATACAGGCAAAACCAATCA
TTTTTTGAATTGGGGGTAGGGGCAGAGATGTGCGAAGGATTGAACAAAGTTTTTGCTCCATAGA
ACACAAGAATCTTTCCCCTAGGCCAAGAGGTTATCGGCCAAAAACCAATCATTCTCTTTTGTGG
AGTATACTCCATAATCCAAAAGAAAATAAATTTAAAATAAAGAACCAAGAATTAAAAATGTAGT
ACAAGGAAAAAGAAAACTATTTAGACCACTATATTTAGACCAGATATCCTATCAAAGTTTGAGG
GCAGTACTGTTAGCTACATTATAATGCCATAAACCAACAAATCCTATATTGGGTAATGGAACGA
TGTAAAATTCTCAAATGATAAGCCTAAATCTGTAAAATCAAAATTGCTTTAAATTACACTTTCC
TAACTACTATAGAATCGAGAAAATGACATAAGAGAACATAAAAGACTACCGTGAATCCTTCACC
ATCTTGTTCAATGGCAAGATCTTGAATAAACCAGACAGCACTCCCTTCATCTCCAACATCATGC
TTGTAATCTAGTAACTCAAATATTAAGCTCTCATCACGTGCAGGGTCCACAAAAACCTCCTTTA
ATTTCAACACACACACACACACAAGAAAAGAAAGACGATCAGATAAATAAACAAATTAAAAATA
AAGACTACCAAAAAGGAAAACAGAGAACGATATACTAACCTGATGATCGGGAACTTGCCTGATG
TTACTGACATC
						
					

			
		

Sequences
Splice junction sequence	CAGATAAATAAACAAATTAAAAATAAAGACTACCAAAAAGGAAAACAGAGAACGATATACTAAC CTGATGATCGGGAACTTGCCTGATGTTACTGACATCtttatcacgatgggctcatatgcagtga tcagaacatctgttcctacttccttaagacgtatgtttgcaagatagaccttcatggatattca tcaagaaa
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	TTTATCACGATGGGCTCATATGCAGTGATCAGAACATCTGTTCCTACTTCCTTAAGACGTATGT TTGCAAGATAGACCTTCATGGATATTCATCAAGAAACCATTTTTCAGTTTAGAACTGAATAAAA ATTGCGAAAGCTGAAAACACAAGAAATGTTACCTTGATAATATTCTGAGCTTCTCTTCCTTGCC GTCCCTTAGAAACAGCCTATGCATAGAAGGGATTAAAAGATATGAGAGACAGAAGGGAAGCAAT GAAAGTCAAGAACTAATATCCAACCACCAATCTAACTGGTGGTATAGTAACCAGGCATTAAGAC ACATATTACTGTCCCAGACAGTCTACAATGAACCATTTGACAGTCTTGGCAATACAGACTAACA ACAATAAAGCAATACCTTTTGCTTTGCTCTAACCAGGAGAAATTGCACCTGGTATTGGGGCGGA TCAATAATAGCAAAAAGTTCATGACATTGAAGATACAAGACATGCACCCAAAATTTAAGATTAA TTGTTTCAGAACTTCCCAACACAAGTTCAGACCATTGCCAACAGTTTTTTTACCTTGCTAATAT CATCTAATCTTACAATTTCAGTCTGTCAATAATCGAAACATGCTATAAAGAAAATATTATTCGT AAGTCTCAGATATGCATCCCCTCTGTATTAACATAAAAATATGCTAGAGGCAATCAACCTAAAT TCTAGCTTTTACACCCGCACATGCATATTCATAATAGCACACACAATGAAGGAACACATTATCA TTTAAGTCAATGAACAGCACATAGAGAGTATCCAATCTGACATAGTCTGGTAGAATTTAAAGGT TACTGTGCCAAGCATGTAGATTAAGATGATAGAATGTTAAGAGTAAGAAATATTGAGACTCGAG GAAGAGAATAAAGTTTGGCAAGGCAGATATTTGTGGACAACAGAAAAATGAAAGTTGAAACTAG TAACAATGGAACTCTAAGCAAATGTCTGCTAAATGACTCATACCATTTGGCCAACTGCAGTTGT AACAATGGCTGGAATGTTTTTGTAACACAATCCAGGAGCCTCAACCACTGTTGACTGCTCAAGC AGCTGAAACATATAGCAAGAAAGAAAATGTTAGGCTAAACCATATCAAGTCTCAAACAGTTGCC AAGCAAACAAAAGAACATGCAGAAACAATGTACCGAATGTCTTTAACATCAATTGGACCTAAGG AAATATGAAAGACAACCAGACCAATGCATAAAACACAGTATCCTAATTGTCAAAGAACAAGTAG AGCTTATAGTTCTTATTTCATTAACAATGATATAGCCAATTAGCCATACAGGCAAAACCAATCA TTTTTTGAATTGGGGGTAGGGGCAGAGATGTGCGAAGGATTGAACAAAGTTTTTGCTCCATAGA ACACAAGAATCTTTCCCCTAGGCCAAGAGGTTATCGGCCAAAAACCAATCATTCTCTTTTGTGG AGTATACTCCATAATCCAAAAGAAAATAAATTTAAAATAAAGAACCAAGAATTAAAAATGTAGT ACAAGGAAAAAGAAAACTATTTAGACCACTATATTTAGACCAGATATCCTATCAAAGTTTGAGG GCAGTACTGTTAGCTACATTATAATGCCATAAACCAACAAATCCTATATTGGGTAATGGAACGA TGTAAAATTCTCAAATGATAAGCCTAAATCTGTAAAATCAAAATTGCTTTAAATTACACTTTCC TAACTACTATAGAATCGAGAAAATGACATAAGAGAACATAAAAGACTACCGTGAATCCTTCACC ATCTTGTTCAATGGCAAGATCTTGAATAAACCAGACAGCACTCCCTTCATCTCCAACATCATGC TTGTAATCTAGTAACTCAAATATTAAGCTCTCATCACGTGCAGGGTCCACAAAAACCTCCTTTA ATTTCAACACACACACACACACAAGAAAAGAAAGACGATCAGATAAATAAACAAATTAAAAATA AAGACTACCAAAAAGGAAAACAGAGAACGATATACTAACCTGATGATCGGGAACTTGCCTGATG TTACTGACATC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS

Conservation Information
Conserved circRNAs	NA
PMCS

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							18670227-18671101(-)
18671247-18669292(+)
						
						Potential amino acid sequence
						
							MAVSKGRQGREAQNIIKVYLANIRLKEVGTDVLITAYEPIVIKMSVTSGKFPIIRRFLWTLHVM
RA*(-)
MIGNLPDVTDIFITMGSYAVIRTSVPTSLRRMFAR*(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	18670227-18671101(-) 18671247-18669292(+)
Potential amino acid sequence	MAVSKGRQGREAQNIIKVYLANIRLKEVGTDVLITAYEPIVIKMSVTSGKFPIIRRFLWTLHVM RA(-) MIGNLPDVTDIFITMGSYAVIRTSVPTSLRRMFAR(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of Cotton_A_33343_circ_g.1

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