Plant Circular RNA Database

Detail information of Zm00001d053262_circ_g.4

			
						 General Information
					
						CircRNA Name
						Zm00001d053262_circ_g.4
					
						ID in PlantcircBase
						zma_circ_008260
					
						Alias
						zma_circ_0001693
					
						Organism
						
							Zea mays						
					
						Position
						
							chr4: 223004340-223010794  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							u-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							Zm00001d053262						
					
						Parent gene annotation
						
							calcium-dependent lipid-binding family protein
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Zm00001d053262_circ_g.3 						
					
						Support reads
						NA
					
						Tissues
						leaf, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d053262_T006:3
Zm00001d053262_T009:1
Zm00001d053262_T001:3
Zm00001d053262_T004:3
Zm00001d053262_T002:3
Zm00001d053262_T008:3

General Information
CircRNA Name	Zm00001d053262_circ_g.4
ID in PlantcircBase	zma_circ_008260
Alias	zma_circ_0001693
Organism	Zea mays
Position	chr4: 223004340-223010794 JBrowse»
Reference genome	AGPv4.38
Type	u-circRNA
Identification method	find_circ
Parent gene	Zm00001d053262
Parent gene annotation	calcium-dependent lipid-binding family protein
Parent gene strand	+
Alternative splicing	Zm00001d053262_circ_g.3
Support reads	NA
Tissues	leaf, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d053262_T006:3 Zm00001d053262_T009:1 Zm00001d053262_T001:3 Zm00001d053262_T004:3 Zm00001d053262_T002:3 Zm00001d053262_T008:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GCTTTCACGTACCGTTGTTGTCATGACCGTGGAAATTCAGTATGCGTTGCTTGAACTTCGCAAT
GGCCCAGATGCAGAGTCACCTCTGGCAGAGCTTGCTttatatacagtttgatcttggtcaattg
aaagtaaggaatgagtttagttggcatggaggtaaagagactgacccttctgctgtaaggcttg
atgttctc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 529
Transcript exons: 223004340-223004448,223004530-223004673,223010519-223010
794
TTATATACAGTTTGATCTTGGTCAATTGAAAGTAAGGAATGAGTTTAGTTGGCATGGAGGTAAA
GAGACTGACCCTTCTGCTGTAAGGCTTGATGTTCTCCATGCAGAAATAAATGGAATCAACATGG
CGGTTGGGGTAAATGGAACTCTGGGAAAGTGTATGATTCGTGATGGTCGCGGCATCAATATAGA
GGTGCGACGTAGCCTACGGGATGTCTTCAAAAAGGTCCCGATACTGTCTATGAAATTTCAGATA
GGATTGCTTCATGGTGTCATGTCAGATAAAGAATACAATGTCGCGATAAGCTGTATTTCGACAA
ATCTGTCCGATACACCTAACCTTCCCCCAAGTTTTCGTGAAAATATGAACAGGACAAAGGAGTC
AATTCGTTTACTTGCAGATAAAGTCAACCTTAGCAACCATTTGTTGCTTTCACGTACCGTTGTT
GTCATGACCGTGGAAATTCAGTATGCGTTGCTTGAACTTCGCAATGGCCCAGATGCAGAGTCAC
CTCTGGCAGAGCTTGCT
						
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	GCTTTCACGTACCGTTGTTGTCATGACCGTGGAAATTCAGTATGCGTTGCTTGAACTTCGCAAT GGCCCAGATGCAGAGTCACCTCTGGCAGAGCTTGCTttatatacagtttgatcttggtcaattg aaagtaaggaatgagtttagttggcatggaggtaaagagactgacccttctgctgtaaggcttg atgttctc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 529 Transcript exons: 223004340-223004448,223004530-223004673,223010519-223010 794 TTATATACAGTTTGATCTTGGTCAATTGAAAGTAAGGAATGAGTTTAGTTGGCATGGAGGTAAA GAGACTGACCCTTCTGCTGTAAGGCTTGATGTTCTCCATGCAGAAATAAATGGAATCAACATGG CGGTTGGGGTAAATGGAACTCTGGGAAAGTGTATGATTCGTGATGGTCGCGGCATCAATATAGA GGTGCGACGTAGCCTACGGGATGTCTTCAAAAAGGTCCCGATACTGTCTATGAAATTTCAGATA GGATTGCTTCATGGTGTCATGTCAGATAAAGAATACAATGTCGCGATAAGCTGTATTTCGACAA ATCTGTCCGATACACCTAACCTTCCCCCAAGTTTTCGTGAAAATATGAACAGGACAAAGGAGTC AATTCGTTTACTTGCAGATAAAGTCAACCTTAGCAACCATTTGTTGCTTTCACGTACCGTTGTT GTCATGACCGTGGAAATTCAGTATGCGTTGCTTGAACTTCGCAATGGCCCAGATGCAGAGTCAC CTCTGGCAGAGCTTGCT
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.126648372

Conservation Information
Conserved circRNAs	NA
PMCS	0.126648372

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							223004546-223004351(+)
223010737-223004351(+)
223004444-223010717(-)
						
						Potential amino acid sequence
						
							MAVGVNGTLGKCMIRDGRGINIEVRRSLRDVFKKVPILSMKFQIGLLHGVMSDKEYNVAISCIS
TNLSDTPNLPPSFRENMNRTKESIRLLADKVNLSNHLLLSRTVVVMTVEIQYALLELRNGPDAE
SPLAELALYTV*(+)
MRCLNFAMAQMQSHLWQSLLYIQFDLGQLKVRNEFSWHGGKETDPSAVRLDVLHAEINGINMAV
GVNGTLGKCMIRDGRGINIEVRRSLRDVFKKVPILSMKFQIGLLHGVMSDKEYNVAISCISTNL
SDTPNLPPSFRENMNRTKESIRLLADKVNLSNHLLLSRTVVVMTVEIQYALLELRNGPDAESPL
AELALYTV*(+)
MENIKPYSRRVSLFTSMPTKLIPYFQLTKIKLYIKQALPEVTLHLGHCEVQATHTEFPRS*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	223004546-223004351(+) 223010737-223004351(+) 223004444-223010717(-)
Potential amino acid sequence	MAVGVNGTLGKCMIRDGRGINIEVRRSLRDVFKKVPILSMKFQIGLLHGVMSDKEYNVAISCIS TNLSDTPNLPPSFRENMNRTKESIRLLADKVNLSNHLLLSRTVVVMTVEIQYALLELRNGPDAE SPLAELALYTV(+) MRCLNFAMAQMQSHLWQSLLYIQFDLGQLKVRNEFSWHGGKETDPSAVRLDVLHAEINGINMAV GVNGTLGKCMIRDGRGINIEVRRSLRDVFKKVPILSMKFQIGLLHGVMSDKEYNVAISCISTNL SDTPNLPPSFRENMNRTKESIRLLADKVNLSNHLLLSRTVVVMTVEIQYALLELRNGPDAESPL AELALYTV(+) MENIKPYSRRVSLFTSMPTKLIPYFQLTKIKLYIKQALPEVTLHLGHCEVQATHTEFPRS*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ma et al., 2021b

circRNA

Detail information of Zm00001d053262_circ_g.4

Address

Any questions?

Supported by