Plant Circular RNA Database

Detail information of AT3G01650_circ_g.4

			
						 General Information
					
						CircRNA Name
						AT3G01650_circ_g.4
					
						ID in PlantcircBase
						ath_circ_019169
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr3: 243254-244348  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							AT3G01650						
					
						Parent gene annotation
						
							E3 ubiquitin-protein ligase RGLG1
							
						Parent gene strand
						+
					
						Alternative splicing
						
							3_circ_ag.1 AT3G01540_circ_g.1 AT3G01540_circ_g.2 AT3G01540_circ_g.3 AT3G01540_circ_g.4 AT3G01540_circ_g.5 AT3G01540_circ_g.6 AT3G01540_circ_g.7 AT3G01560_circ_g.1 AT3G01560_circ_g.2 AT3G01560_circ_g.3 AT3G01560_circ_g.4 AT3G01560_circ_g.5 AT3G01560_circ_g.6 AT3G01590_circ_g.1 AT3G01590_circ_g.2 AT3G01590_circ_g.3 AT3G01590_circ_g.4 AT3G01600_circ_g.1 AT3G01610_circ_g.1 AT3G01640_circ_g.1 AT3G01650_circ_g.1 AT3G01650_circ_g.2 AT3G01650_circ_g.3 AT3G01650_circ_g.5 AT3G01650_circ_g.6 AT3G01670_circ_g.1 AT3G01670_circ_g.2 AT3G01670_circ_g.3 AT3G01680_circ_g.1 AT3G01690_circ_g.1 AT3G01690_circ_g.2 AT3G01710_circ_g.1 AT3G01720_circ_g.1 AT3G01720_circ_g.2 AT3G01770_circ_g.1 AT3G01770_circ_g.2 						
					
						Support reads
						6
					
						Tissues
						seedlings
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT3G01650.2:6
AT3G01650.1:6

General Information
CircRNA Name	AT3G01650_circ_g.4
ID in PlantcircBase	ath_circ_019169
Alias	NA
Organism	Arabidpsis thaliana
Position	chr3: 243254-244348 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer
Parent gene	AT3G01650
Parent gene annotation	E3 ubiquitin-protein ligase RGLG1
Parent gene strand	+
Alternative splicing	3_circ_ag.1 AT3G01540_circ_g.1 AT3G01540_circ_g.2 AT3G01540_circ_g.3 AT3G01540_circ_g.4 AT3G01540_circ_g.5 AT3G01540_circ_g.6 AT3G01540_circ_g.7 AT3G01560_circ_g.1 AT3G01560_circ_g.2 AT3G01560_circ_g.3 AT3G01560_circ_g.4 AT3G01560_circ_g.5 AT3G01560_circ_g.6 AT3G01590_circ_g.1 AT3G01590_circ_g.2 AT3G01590_circ_g.3 AT3G01590_circ_g.4 AT3G01600_circ_g.1 AT3G01610_circ_g.1 AT3G01640_circ_g.1 AT3G01650_circ_g.1 AT3G01650_circ_g.2 AT3G01650_circ_g.3 AT3G01650_circ_g.5 AT3G01650_circ_g.6 AT3G01670_circ_g.1 AT3G01670_circ_g.2 AT3G01670_circ_g.3 AT3G01680_circ_g.1 AT3G01690_circ_g.1 AT3G01690_circ_g.2 AT3G01710_circ_g.1 AT3G01720_circ_g.1 AT3G01720_circ_g.2 AT3G01770_circ_g.1 AT3G01770_circ_g.2
Support reads	6
Tissues	seedlings
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT3G01650.2:6 AT3G01650.1:6

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TCCTTTGTCAATAGTCTTAGTTGGGGTCGGGGATGGACCATGGGACATGATGCAGGAATTTGAT
GATAACATACCTGCCCGAGCTTTCGATAACTTCCAGgtgtccgaggctctaggccgtgcaggtc
ttgaatcttctaatctaatcgttggtattgatttcaccaagagcaatgaatggacaggtttgtt
atattatt

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GTGTCCGAGGCTCTAGGCCGTGCAGGTCTTGAATCTTCTAATCTAATCGTTGGTATTGATTTCA
CCAAGAGCAATGAATGGACAGGTTTGTTATATTATTCTTATTCTTACGTTTTGAATGTAAAGGA
GAAGCCAAACTAATTGGTTTTGGTACTGCAGGGGCCAAGTCCTTCAATAGGAAAAGCTTGCATC
ACTTGAGCAATACCCCGAATCCATACGAGCAAGCAATTACTATCATTGGAAGAACCTTAGCCGC
CTTTGACGAGGATAACTTAATTCCTTGTTATGGTTTTGGAGACGGTATGCACTGTTTTCTCTCT
TCTCGATTCTCATAAGAATGTATATCAATCGGATCATCATTGACTTGCTTTCATCTCTTTGCAG
CATCAACTCATGATCAAGACGTGTTTAGTTTCTATCCAGAGGGAAGATTCTGTAATGGATTTGA
GGAAGTATTGGCTCGTTATAGAGAAATTGTACCTCAGCTTAAGCTCGCAGGTTTATTTCCTGCT
CTGACCTGCACTGGCCAATTCTATATCTCCTTGTGAAACGCAAGTTATAAACTTAAAACTGTGT
TTTTTTTTCCTGCGAACTTATGTATTTCAGGACCAACTTCTTTTGCCCCGATCATTGAAATGGC
AATGACAGTGGTTGAGCAGAGTAGTGGCCAATATCATGTGTTAGTGATTATAGCTGATGGCCAG
GTACTGATTAATATTTTGACTGCCTAAGCTGTGTATGATCTTCTGGTTTTGTGTCTAGGGATAT
CAAAGTGGACCCATTTTGTTGTTTCAGGTCACAAGAAGTGTCGATACCGAACACGGGCGGCTAA
GTCCGCAAGAACAGAAGACAGTAGATGCAATTGTGAAAGCAAGGTGCGAATATTTCAATCTTTT
GACTAATGTTTCTTCCATTTTAAAGTTCTTTACTTTGAGTGTAATGTTATAAAGTGTTATGGTA
TGTAATCAGTTTCATCCTCTCTATTACAGTACCCTTCCTTTGTCAATAGTCTTAGTTGGGGTCG
GGGATGGACCATGGGACATGATGCAGGAATTTGATGATAACATACCTGCCCGAGCTTTCGATAA
CTTCCAG

Sequences
Splice junction sequence	TCCTTTGTCAATAGTCTTAGTTGGGGTCGGGGATGGACCATGGGACATGATGCAGGAATTTGAT GATAACATACCTGCCCGAGCTTTCGATAACTTCCAGgtgtccgaggctctaggccgtgcaggtc ttgaatcttctaatctaatcgttggtattgatttcaccaagagcaatgaatggacaggtttgtt atattatt
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GTGTCCGAGGCTCTAGGCCGTGCAGGTCTTGAATCTTCTAATCTAATCGTTGGTATTGATTTCA CCAAGAGCAATGAATGGACAGGTTTGTTATATTATTCTTATTCTTACGTTTTGAATGTAAAGGA GAAGCCAAACTAATTGGTTTTGGTACTGCAGGGGCCAAGTCCTTCAATAGGAAAAGCTTGCATC ACTTGAGCAATACCCCGAATCCATACGAGCAAGCAATTACTATCATTGGAAGAACCTTAGCCGC CTTTGACGAGGATAACTTAATTCCTTGTTATGGTTTTGGAGACGGTATGCACTGTTTTCTCTCT TCTCGATTCTCATAAGAATGTATATCAATCGGATCATCATTGACTTGCTTTCATCTCTTTGCAG CATCAACTCATGATCAAGACGTGTTTAGTTTCTATCCAGAGGGAAGATTCTGTAATGGATTTGA GGAAGTATTGGCTCGTTATAGAGAAATTGTACCTCAGCTTAAGCTCGCAGGTTTATTTCCTGCT CTGACCTGCACTGGCCAATTCTATATCTCCTTGTGAAACGCAAGTTATAAACTTAAAACTGTGT TTTTTTTTCCTGCGAACTTATGTATTTCAGGACCAACTTCTTTTGCCCCGATCATTGAAATGGC AATGACAGTGGTTGAGCAGAGTAGTGGCCAATATCATGTGTTAGTGATTATAGCTGATGGCCAG GTACTGATTAATATTTTGACTGCCTAAGCTGTGTATGATCTTCTGGTTTTGTGTCTAGGGATAT CAAAGTGGACCCATTTTGTTGTTTCAGGTCACAAGAAGTGTCGATACCGAACACGGGCGGCTAA GTCCGCAAGAACAGAAGACAGTAGATGCAATTGTGAAAGCAAGGTGCGAATATTTCAATCTTTT GACTAATGTTTCTTCCATTTTAAAGTTCTTTACTTTGAGTGTAATGTTATAAAGTGTTATGGTA TGTAATCAGTTTCATCCTCTCTATTACAGTACCCTTCCTTTGTCAATAGTCTTAGTTGGGGTCG GGGATGGACCATGGGACATGATGCAGGAATTTGATGATAACATACCTGCCCGAGCTTTCGATAA CTTCCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.22085053

Conservation Information
Conserved circRNAs	NA
PMCS	0.22085053

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							243889-244345(+)
						
						Potential amino acid sequence
						
							MAMTVVEQSSGQYHVLVIIADGQVTRSVDTEHGRLSPQEQKTVDAIVKASTLPLSIVLVGVGDG
PWDMMQEFDDNIPARAFDNFQVSEALGRAGLESSNLIVGIDFTKSNEWTGAKSFNRKSLHHLSN
TPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFYPEGRFCNGFEEVLARYREIV
PQLKLAGPTSFAPIIEMAMTVVEQSSGQYHVLVIIADGQVTRSVDTEHGRLSPQEQKTVDAIVK
ASTLPLSIVLVGVGDGPWDMMQEFDDNIPARAFDNFQVSEALGRAGLESSNLIVGIDFTKSNEW
TGAKSFNRKSLHHLSNTPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFYPEGR
FCNGFEEVLARYREIVPQLKLAGPTSFAPIIEMAMTVVEQSSGQYHVLVIIADGQVTRSVDTEH
GRLSPQEQKTVDAIVKASTLPLSIVLVGVGDGPWDMMQEFDDNIPARAFDNFQVSEALGRAGLE
SSNLIVGIDFTKSNEWTGAKSFNRKSLHHLSNTPNPYEQAITIIGRTLAAFDEDNLIPCYGFGD
ASTHDQDVFSFYPEGRFCNGFEEVLARYREIVPQLKLAGPTSFAPIIEMAMTVVEQSSGQYHVL
VIIADGQVTRSVDTEHGRLSPQEQKTVDAIVKASTLPLSIVLVGVGDGPWDMMQEFDDNIPARA
FDNFQ(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to heat stress

Functional Information
Coding potential	Y
Potential coding position	243889-244345(+)
Potential amino acid sequence	MAMTVVEQSSGQYHVLVIIADGQVTRSVDTEHGRLSPQEQKTVDAIVKASTLPLSIVLVGVGDG PWDMMQEFDDNIPARAFDNFQVSEALGRAGLESSNLIVGIDFTKSNEWTGAKSFNRKSLHHLSN TPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFYPEGRFCNGFEEVLARYREIV PQLKLAGPTSFAPIIEMAMTVVEQSSGQYHVLVIIADGQVTRSVDTEHGRLSPQEQKTVDAIVK ASTLPLSIVLVGVGDGPWDMMQEFDDNIPARAFDNFQVSEALGRAGLESSNLIVGIDFTKSNEW TGAKSFNRKSLHHLSNTPNPYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFYPEGR FCNGFEEVLARYREIVPQLKLAGPTSFAPIIEMAMTVVEQSSGQYHVLVIIADGQVTRSVDTEH GRLSPQEQKTVDAIVKASTLPLSIVLVGVGDGPWDMMQEFDDNIPARAFDNFQVSEALGRAGLE SSNLIVGIDFTKSNEWTGAKSFNRKSLHHLSNTPNPYEQAITIIGRTLAAFDEDNLIPCYGFGD ASTHDQDVFSFYPEGRFCNGFEEVLARYREIVPQLKLAGPTSFAPIIEMAMTVVEQSSGQYHVL VIIADGQVTRSVDTEHGRLSPQEQKTVDAIVKASTLPLSIVLVGVGDGPWDMMQEFDDNIPARA FDNFQ(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to heat stress

			
						 Other Information
					
						References
						Pan et al., 2017

circRNA

Detail information of AT3G01650_circ_g.4

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