Plant Circular RNA Database

Detail information of AT5G14060_circ_g.12

			
						 General Information
					
						CircRNA Name
						AT5G14060_circ_g.12
					
						ID in PlantcircBase
						ath_circ_038180
					
						Alias
						Ath_circ_FC3294
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 4537285-4538664  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						PcircRNA_finder
					
						Parent gene
						
							AT5G14060						
					
						Parent gene annotation
						
							Aspartokinase
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT5G14060_circ_g.2 AT5G14060_circ_g.3 AT5G14060_circ_g.4 AT5G14060_circ_g.5 AT5G14060_circ_g.6 AT5G14060_circ_g.7 AT5G14060_circ_g.8 AT5G14060_circ_g.9 AT5G14060_circ_g.10 AT5G14060_circ_g.11 AT5G14060_circ_g.13 AT5G14060_circ_g.14 AT5G14060_circ_g.15 AT5G14060_circ_g.16 AT5G14060_circ_g.17 AT5G14060_circ_g.18 AT5G14060_circ_g.19 AT5G14060_circ_g.20 AT5G14060_circ_g.21 AT5G14060_circ_g.22 						
					
						Support reads
						8
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G14060.1:7
AT5G14060.3:6
AT5G14060.2:7

General Information
CircRNA Name	AT5G14060_circ_g.12
ID in PlantcircBase	ath_circ_038180
Alias	Ath_circ_FC3294
Organism	Arabidpsis thaliana
Position	chr5: 4537285-4538664 JBrowse»
Reference genome	TAIR10.38
Type	ue-circRNA
Identification method	PcircRNA_finder
Parent gene	AT5G14060
Parent gene annotation	Aspartokinase
Parent gene strand	+
Alternative splicing	AT5G14060_circ_g.2 AT5G14060_circ_g.3 AT5G14060_circ_g.4 AT5G14060_circ_g.5 AT5G14060_circ_g.6 AT5G14060_circ_g.7 AT5G14060_circ_g.8 AT5G14060_circ_g.9 AT5G14060_circ_g.10 AT5G14060_circ_g.11 AT5G14060_circ_g.13 AT5G14060_circ_g.14 AT5G14060_circ_g.15 AT5G14060_circ_g.16 AT5G14060_circ_g.17 AT5G14060_circ_g.18 AT5G14060_circ_g.19 AT5G14060_circ_g.20 AT5G14060_circ_g.21 AT5G14060_circ_g.22
Support reads	8
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G14060.1:7 AT5G14060.3:6 AT5G14060.2:7

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							GGAAGAGCTAGAGAAGATTGCTGTTGTGAAACTACTTCAACGCAGATCAATCATCTCTCTCATA
GGAAATGTTCAGAAATCCTCACTCATATTAGAGAAGtatgatgcatttgagatcggatttatca
ccacagacgactttactaatgctgatatacttgaagcaacataccctgccgtctcaaaaacact
tgttggcg

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 816
Transcript exons: 4537285-4537434,4537608-4537703,4537775-4537891,4537973-
4538092,4538170-4538262,4538343-4538465,4538548-4538664
TATGATGCATTTGAGATCGGATTTATCACCACAGACGACTTTACTAATGCTGATATACTTGAAG
CAACATACCCTGCCGTCTCAAAAACACTTGTTGGCGACTGGAGCAAGGAAAATGCAGTCCCTGT
TGTTACTGGCTACCTCGGAAAGGGATGGAGGTCTTGTGCTATCACTACGCTGGGTAGGGGTGGC
AGTGATTTGACAGCTACAACGATTGGGAAAGCGCTAGGACTGCGGGAAATTCAGGTTTGGAAAG
ATGTGGATGGAGTTTTGACTTGTGATCCTAACATATATCCTGGAGCTCAATCTGTTCCGTACTT
AACGTTCGATGAGGCAGCTGAGCTTGCTTACTTTGGTGCCCAGGTGTTGCATCCACTGTCTATG
AGGCCAGCAAGAGATGGCGACATTCCTGTGAGGGTTAAGAACTCGTACAACCCCACTGCTCCAG
GAACTGTCATCACCAGATCAAGAGACATGAGTAAGGCTGTGCTAACCAGCATTGTTCTGAAACG
TAATGTGACCATGTTGGACATAGCAAGCACCCGTATGCTTGGCCAATATGGTTTCCTTGCCAAG
GTGTTTACCACATTTGAAGATTTAGGTATATCGGTTGATGTTGTGGCAACAAGTGAAGTTAGTA
TATCATTGACATTGGATCCAGCAAAGCTCTGGGGTAGAGAGTTAATTCAGCGAGTAAATGAACT
GGATAATTTGGTGGAAGAGCTAGAGAAGATTGCTGTTGTGAAACTACTTCAACGCAGATCAATC
ATCTCTCTCATAGGAAATGTTCAGAAATCCTCACTCATATTAGAGAAG
						
					

						Genomic sequence
						
							TATGATGCATTTGAGATCGGATTTATCACCACAGACGACTTTACTAATGCTGATATACTTGAAG
CAACATACCCTGCCGTCTCAAAAACACTTGTTGGCGACTGGAGCAAGGAAAATGCAGTCCCTGT
TGTTACTGGCTACCTCGGAAAGGTTTATATTTTTCTGACTTTTTATGTTGTTCTCGTGGATCAA
GAAATATGTGGTGCTCAACTCGGGTTCTCATCTATTCTATAAATTGGAATGGGTTCTGCACAAG
AGTTGTTTTGCTTATTGTATCAATTTTGAAAACAAAACTTCATTCTCGTATGCATGTGTTTCTG
CAGGGATGGAGGTCTTGTGCTATCACTACGCTGGGTAGGGGTGGCAGTGATTTGACAGCTACAA
CGATTGGGAAAGCGCTAGGACTGCGGGAAATTCAGGTATTTGATGGCTTTGGCCAAGATTGCAT
CATGTAGATGAAATTTTGTTAAACAATGATTTTTTAATATAGGTTTGGAAAGATGTGGATGGAG
TTTTGACTTGTGATCCTAACATATATCCTGGAGCTCAATCTGTTCCGTACTTAACGTTCGATGA
GGCAGCTGAGCTTGCTTACTTTGGTGCCCAGGTAATCTCGTAGATTAGCTATGCACTATGCTGA
TGAGTTCTTTTCTGTCAGATGGCTGAGCATATTCCTCTTCCATTGCAGGTGTTGCATCCACTGT
CTATGAGGCCAGCAAGAGATGGCGACATTCCTGTGAGGGTTAAGAACTCGTACAACCCCACTGC
TCCAGGAACTGTCATCACCAGATCAAGAGACATGAGTAAGGTTCGTGAAACAGATGTGTTTTTT
TAACTCCTTATATCAAAATATCAATGACCTTTTCCTCCTTTAATTCCTTGTAGGCTGTGCTAAC
CAGCATTGTTCTGAAACGTAATGTGACCATGTTGGACATAGCAAGCACCCGTATGCTTGGCCAA
TATGGTTTCCTTGCCAAGGTAATATAATTGGATAATGATATCTTTATTCGACTTTGTTTAATAT
CTCACTGGAAGCATACTCACTGTCTCGTGTGCAGGTGTTTACCACATTTGAAGATTTAGGTATA
TCGGTTGATGTTGTGGCAACAAGTGAAGTTAGTATATCATTGACATTGGATCCAGCAAAGCTCT
GGGGTAGAGAGTTAATTCAGCGAGTAAATGTAATACTCCTTTGACTTTGATAGATTGTTTCTTT
TGTGAGGGTCATATGTGGTATTGGTTAAAGGGACTGGGTGTGAACAGGAACTGGATAATTTGGT
GGAAGAGCTAGAGAAGATTGCTGTTGTGAAACTACTTCAACGCAGATCAATCATCTCTCTCATA
GGAAATGTTCAGAAATCCTCACTCATATTAGAGAAG
						
					

			
		

Sequences
Splice junction sequence	GGAAGAGCTAGAGAAGATTGCTGTTGTGAAACTACTTCAACGCAGATCAATCATCTCTCTCATA GGAAATGTTCAGAAATCCTCACTCATATTAGAGAAGtatgatgcatttgagatcggatttatca ccacagacgactttactaatgctgatatacttgaagcaacataccctgccgtctcaaaaacact tgttggcg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 816 Transcript exons: 4537285-4537434,4537608-4537703,4537775-4537891,4537973- 4538092,4538170-4538262,4538343-4538465,4538548-4538664 TATGATGCATTTGAGATCGGATTTATCACCACAGACGACTTTACTAATGCTGATATACTTGAAG CAACATACCCTGCCGTCTCAAAAACACTTGTTGGCGACTGGAGCAAGGAAAATGCAGTCCCTGT TGTTACTGGCTACCTCGGAAAGGGATGGAGGTCTTGTGCTATCACTACGCTGGGTAGGGGTGGC AGTGATTTGACAGCTACAACGATTGGGAAAGCGCTAGGACTGCGGGAAATTCAGGTTTGGAAAG ATGTGGATGGAGTTTTGACTTGTGATCCTAACATATATCCTGGAGCTCAATCTGTTCCGTACTT AACGTTCGATGAGGCAGCTGAGCTTGCTTACTTTGGTGCCCAGGTGTTGCATCCACTGTCTATG AGGCCAGCAAGAGATGGCGACATTCCTGTGAGGGTTAAGAACTCGTACAACCCCACTGCTCCAG GAACTGTCATCACCAGATCAAGAGACATGAGTAAGGCTGTGCTAACCAGCATTGTTCTGAAACG TAATGTGACCATGTTGGACATAGCAAGCACCCGTATGCTTGGCCAATATGGTTTCCTTGCCAAG GTGTTTACCACATTTGAAGATTTAGGTATATCGGTTGATGTTGTGGCAACAAGTGAAGTTAGTA TATCATTGACATTGGATCCAGCAAAGCTCTGGGGTAGAGAGTTAATTCAGCGAGTAAATGAACT GGATAATTTGGTGGAAGAGCTAGAGAAGATTGCTGTTGTGAAACTACTTCAACGCAGATCAATC ATCTCTCTCATAGGAAATGTTCAGAAATCCTCACTCATATTAGAGAAG
Genomic sequence	TATGATGCATTTGAGATCGGATTTATCACCACAGACGACTTTACTAATGCTGATATACTTGAAG CAACATACCCTGCCGTCTCAAAAACACTTGTTGGCGACTGGAGCAAGGAAAATGCAGTCCCTGT TGTTACTGGCTACCTCGGAAAGGTTTATATTTTTCTGACTTTTTATGTTGTTCTCGTGGATCAA GAAATATGTGGTGCTCAACTCGGGTTCTCATCTATTCTATAAATTGGAATGGGTTCTGCACAAG AGTTGTTTTGCTTATTGTATCAATTTTGAAAACAAAACTTCATTCTCGTATGCATGTGTTTCTG CAGGGATGGAGGTCTTGTGCTATCACTACGCTGGGTAGGGGTGGCAGTGATTTGACAGCTACAA CGATTGGGAAAGCGCTAGGACTGCGGGAAATTCAGGTATTTGATGGCTTTGGCCAAGATTGCAT CATGTAGATGAAATTTTGTTAAACAATGATTTTTTAATATAGGTTTGGAAAGATGTGGATGGAG TTTTGACTTGTGATCCTAACATATATCCTGGAGCTCAATCTGTTCCGTACTTAACGTTCGATGA GGCAGCTGAGCTTGCTTACTTTGGTGCCCAGGTAATCTCGTAGATTAGCTATGCACTATGCTGA TGAGTTCTTTTCTGTCAGATGGCTGAGCATATTCCTCTTCCATTGCAGGTGTTGCATCCACTGT CTATGAGGCCAGCAAGAGATGGCGACATTCCTGTGAGGGTTAAGAACTCGTACAACCCCACTGC TCCAGGAACTGTCATCACCAGATCAAGAGACATGAGTAAGGTTCGTGAAACAGATGTGTTTTTT TAACTCCTTATATCAAAATATCAATGACCTTTTCCTCCTTTAATTCCTTGTAGGCTGTGCTAAC CAGCATTGTTCTGAAACGTAATGTGACCATGTTGGACATAGCAAGCACCCGTATGCTTGGCCAA TATGGTTTCCTTGCCAAGGTAATATAATTGGATAATGATATCTTTATTCGACTTTGTTTAATAT CTCACTGGAAGCATACTCACTGTCTCGTGTGCAGGTGTTTACCACATTTGAAGATTTAGGTATA TCGGTTGATGTTGTGGCAACAAGTGAAGTTAGTATATCATTGACATTGGATCCAGCAAAGCTCT GGGGTAGAGAGTTAATTCAGCGAGTAAATGTAATACTCCTTTGACTTTGATAGATTGTTTCTTT TGTGAGGGTCATATGTGGTATTGGTTAAAGGGACTGGGTGTGAACAGGAACTGGATAATTTGGT GGAAGAGCTAGAGAAGATTGCTGTTGTGAAACTACTTCAACGCAGATCAATCATCTCTCTCATA GGAAATGTTCAGAAATCCTCACTCATATTAGAGAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.214667119

Conservation Information
Conserved circRNAs	NA
PMCS	0.214667119

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							4537991-4538661(+)
						
						Potential amino acid sequence
						
							MRPARDGDIPVRVKNSYNPTAPGTVITRSRDMSKAVLTSIVLKRNVTMLDIASTRMLGQYGFLA
KVFTTFEDLGISVDVVATSEVSISLTLDPAKLWGRELIQRVNELDNLVEELEKIAVVKLLQRRS
IISLIGNVQKSSLILEKYDAFEIGFITTDDFTNADILEATYPAVSKTLVGDWSKENAVPVVTGY
LGKGWRSCAITTLGRGGSDLTATTIGKALGLREIQVWKDVDGVLTCDPNIYPGAQSVPYLTFDE
AAELAYFGAQVLHPLSMRPARDGDIPVRVKNSYNPTAPGTVITRSRDMSKAVLTSIVLKRNVTM
LDIASTRMLGQYGFLAKVFTTFEDLGISVDVVATSEVSISLTLDPAKLWGRELIQRVNELDNLV
EELEKIAVVKLLQRRSIISLIGNVQKSSLILEKYDAFEIGFITTDDFTNADILEATYPAVSKTL
VGDWSKENAVPVVTGYLGKGWRSCAITTLGRGGSDLTATTIGKALGLREIQVWKDVDGVLTCDP
NIYPGAQSVPYLTFDEAAELAYFGAQVLHPLSMRPARDGDIPVRVKNSYNPTAPGTVITRSRDM
SKAVLTSIVLKRNVTMLDIASTRMLGQYGFLAKVFTTFEDLGISVDVVATSEVSISLTLDPAKL
WGRELIQRVNELDNLVEELEKIAVVKLLQRRSIISLIGNVQKSSLILEKYDAFEIGFITTDDFT
NADILEATYPAVSKTLVGDWSKENAVPVVTGYLGKGWRSCAITTLGRGGSDLTATTIGKALGLR
EIQVWKDVDGVLTCDPNIYPGAQSVPYLTFDEAAELAYFGAQVLHPLSMRPARDGDIPVRVKNS
YNPTAPGTVITRSRDMSKAVLTSIVLKRNVTMLDIASTRMLGQYGFLAKVFTTFEDLGISVDVV
ATSEVSISLTLDPAKLWGRELIQRVNELDNLVEELEKIAVVKLLQRRSIISLIGNVQKSSLILE
K(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	4537991-4538661(+)
Potential amino acid sequence	MRPARDGDIPVRVKNSYNPTAPGTVITRSRDMSKAVLTSIVLKRNVTMLDIASTRMLGQYGFLA KVFTTFEDLGISVDVVATSEVSISLTLDPAKLWGRELIQRVNELDNLVEELEKIAVVKLLQRRS IISLIGNVQKSSLILEKYDAFEIGFITTDDFTNADILEATYPAVSKTLVGDWSKENAVPVVTGY LGKGWRSCAITTLGRGGSDLTATTIGKALGLREIQVWKDVDGVLTCDPNIYPGAQSVPYLTFDE AAELAYFGAQVLHPLSMRPARDGDIPVRVKNSYNPTAPGTVITRSRDMSKAVLTSIVLKRNVTM LDIASTRMLGQYGFLAKVFTTFEDLGISVDVVATSEVSISLTLDPAKLWGRELIQRVNELDNLV EELEKIAVVKLLQRRSIISLIGNVQKSSLILEKYDAFEIGFITTDDFTNADILEATYPAVSKTL VGDWSKENAVPVVTGYLGKGWRSCAITTLGRGGSDLTATTIGKALGLREIQVWKDVDGVLTCDP NIYPGAQSVPYLTFDEAAELAYFGAQVLHPLSMRPARDGDIPVRVKNSYNPTAPGTVITRSRDM SKAVLTSIVLKRNVTMLDIASTRMLGQYGFLAKVFTTFEDLGISVDVVATSEVSISLTLDPAKL WGRELIQRVNELDNLVEELEKIAVVKLLQRRSIISLIGNVQKSSLILEKYDAFEIGFITTDDFT NADILEATYPAVSKTLVGDWSKENAVPVVTGYLGKGWRSCAITTLGRGGSDLTATTIGKALGLR EIQVWKDVDGVLTCDPNIYPGAQSVPYLTFDEAAELAYFGAQVLHPLSMRPARDGDIPVRVKNS YNPTAPGTVITRSRDMSKAVLTSIVLKRNVTMLDIASTRMLGQYGFLAKVFTTFEDLGISVDVV ATSEVSISLTLDPAKLWGRELIQRVNELDNLVEELEKIAVVKLLQRRSIISLIGNVQKSSLILE K(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017;Chen et al., 2017a

circRNA

Detail information of AT5G14060_circ_g.12

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