Plant Circular RNA Database

Detail information of Zm00001d046020_circ_g.2

			
						 General Information
					
						CircRNA Name
						Zm00001d046020_circ_g.2
					
						ID in PlantcircBase
						zma_circ_009935
					
						Alias
						Zm09circ00034, zma_circ_0002950, GRMZM2G077844_C1
					
						Organism
						
							Zea mays						
					
						Position
						
							chr9: 55678041-55678409  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI2, find_circ
					
						Parent gene
						
							Zm00001d046020						
					
						Parent gene annotation
						
							Putative VHS/GAT domain containing family protein  
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Zm00001d046020_circ_g.1 						
					
						Support reads
						NA
					
						Tissues
						leaf, endosperm, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d046020_T001:2
Zm00001d046020_T002:2
Zm00001d046020_T005:2
Zm00001d046020_T006:2
Zm00001d046020_T004:2
Zm00001d046020_T003:2
Zm00001d046020_T008:2

General Information
CircRNA Name	Zm00001d046020_circ_g.2
ID in PlantcircBase	zma_circ_009935
Alias	Zm09circ00034, zma_circ_0002950, GRMZM2G077844_C1
Organism	Zea mays
Position	chr9: 55678041-55678409 JBrowse»
Reference genome	AGPv4.38
Type	e-circRNA
Identification method	CIRI2, find_circ
Parent gene	Zm00001d046020
Parent gene annotation	Putative VHS/GAT domain containing family protein
Parent gene strand	-
Alternative splicing	Zm00001d046020_circ_g.1
Support reads	NA
Tissues	leaf, endosperm, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d046020_T001:2 Zm00001d046020_T002:2 Zm00001d046020_T005:2 Zm00001d046020_T006:2 Zm00001d046020_T004:2 Zm00001d046020_T003:2 Zm00001d046020_T008:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GCACAAGGTAGAAAATACCTCTCTGTTACCAGGATCGATAGCATTTAACATCTCTGAAAGAACA
TCCATGACCCCACGTGCATTCTGAATTTCTGTCAAGgatgttgagttgacaagctgcaccactc
tttgcttgtatgaacgacattggtccacaaggtccacaatgacctcctgccttagaccctgcat
gttgagta

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 168
Transcript exons: 55678041-55678126,55678328-55678409
GATGTTGAGTTGACAAGCTGCACCACTCTTTGCTTGTATGAACGACATTGGTCCACAAGGTCCA
CAATGACCTCCTGCCTTAGACCCTCTCTGTTACCAGGATCGATAGCATTTAACATCTCTGAAAG
AACATCCATGACCCCACGTGCATTCTGAATTTCTGTCAAG
						
						Genomic sequence
						
							GATGTTGAGTTGACAAGCTGCACCACTCTTTGCTTGTATGAACGACATTGGTCCACAAGGTCCA
CAATGACCTCCTGCCTTAGACCCTGCATGTTGAGTACTATTTAGAAATCCAGGGATGACTAGAA
CTTCTGGAACTGTATGGTCCAGTTGAACAATCTGGAACATGTCAACATACAATCCTGTTTAAAA
GAAAATAAATTCGCAAGGAAAATGGGAAGGTCATCTCATGATTTTACTAGACGTGTGTAATTAT
CAAGGGTAAGTAAGCACAAGGTAGAAAATACCTCTCTGTTACCAGGATCGATAGCATTTAACAT
CTCTGAAAGAACATCCATGACCCCACGTGCATTCTGAATTTCTGTCAAG

Sequences
Splice junction sequence	GCACAAGGTAGAAAATACCTCTCTGTTACCAGGATCGATAGCATTTAACATCTCTGAAAGAACA TCCATGACCCCACGTGCATTCTGAATTTCTGTCAAGgatgttgagttgacaagctgcaccactc tttgcttgtatgaacgacattggtccacaaggtccacaatgacctcctgccttagaccctgcat gttgagta
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 168 Transcript exons: 55678041-55678126,55678328-55678409 GATGTTGAGTTGACAAGCTGCACCACTCTTTGCTTGTATGAACGACATTGGTCCACAAGGTCCA CAATGACCTCCTGCCTTAGACCCTCTCTGTTACCAGGATCGATAGCATTTAACATCTCTGAAAG AACATCCATGACCCCACGTGCATTCTGAATTTCTGTCAAG
Genomic sequence	GATGTTGAGTTGACAAGCTGCACCACTCTTTGCTTGTATGAACGACATTGGTCCACAAGGTCCA CAATGACCTCCTGCCTTAGACCCTGCATGTTGAGTACTATTTAGAAATCCAGGGATGACTAGAA CTTCTGGAACTGTATGGTCCAGTTGAACAATCTGGAACATGTCAACATACAATCCTGTTTAAAA GAAAATAAATTCGCAAGGAAAATGGGAAGGTCATCTCATGATTTTACTAGACGTGTGTAATTAT CAAGGGTAAGTAAGCACAAGGTAGAAAATACCTCTCTGTTACCAGGATCGATAGCATTTAACAT CTCTGAAAGAACATCCATGACCCCACGTGCATTCTGAATTTCTGTCAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.300955775

Conservation Information
Conserved circRNAs	NA
PMCS	0.300955775

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							55678090-55678337(-)
55678380-55678043(-)
						
						Potential amino acid sequence
						
							MSFIQAKSGAACQLNILDRNSECTWGHGCSFRDVKCYRSW*(-)
MDVLSEMLNAIDPGNREGLRQEVIVDLVDQCRSYKQRVVQLVNSTSLTEIQNARGVMDVLSEML
NAIDPGNREGLRQEVIVDLVDQCRSYKQRVVQLVNSTSLTEIQNARGVMDVLSEMLNAIDPGNR
EGLRQEVIVDLVDQCRSYKQRVVQLVNSTSLTEIQNARGVMDVLSEMLNAIDPGNREGLRQEVI
VDLVDQCRSYKQRVVQLVNST(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to drought stress

Functional Information
Coding potential	Y
Potential coding position	55678090-55678337(-) 55678380-55678043(-)
Potential amino acid sequence	MSFIQAKSGAACQLNILDRNSECTWGHGCSFRDVKCYRSW*(-) MDVLSEMLNAIDPGNREGLRQEVIVDLVDQCRSYKQRVVQLVNSTSLTEIQNARGVMDVLSEML NAIDPGNREGLRQEVIVDLVDQCRSYKQRVVQLVNSTSLTEIQNARGVMDVLSEMLNAIDPGNR EGLRQEVIVDLVDQCRSYKQRVVQLVNSTSLTEIQNARGVMDVLSEMLNAIDPGNREGLRQEVI VDLVDQCRSYKQRVVQLVNST(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to drought stress

			
						 Other Information
					
						References
						Han et al., 2020; Ma et al., 2021b;Zhang et al., 2019

circRNA

Detail information of Zm00001d046020_circ_g.2

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