Plant Circular RNA Database

Detail information of Zm00001d047656_circ_ag.1

			
						 General Information
					
						CircRNA Name
						Zm00001d047656_circ_ag.1
					
						ID in PlantcircBase
						zma_circ_010052
					
						Alias
						zma_circ_0003086
					
						Organism
						
							Zea mays						
					
						Position
						
							chr9: 138272860-138349836  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							ag-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							NA						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						NA
					
						Alternative splicing
						
							NA						
					
						Support reads
						NA
					
						Tissues
						leaf, root
					
						Exon boundary  
						
							NA-NA							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						NA

General Information
CircRNA Name	Zm00001d047656_circ_ag.1
ID in PlantcircBase	zma_circ_010052
Alias	zma_circ_0003086
Organism	Zea mays
Position	chr9: 138272860-138349836 JBrowse»
Reference genome	AGPv4.38
Type	ag-circRNA
Identification method	find_circ
Parent gene	NA
Parent gene annotation	NA
Parent gene strand	NA
Alternative splicing	NA
Support reads	NA
Tissues	leaf, root
Exon boundary	NA-NA "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	NA

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							Yes							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							F: GAACTGCCGTAACTCCCTTCTAT
 R: CATGACGAGGCTGGTGAACA
PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	Yes Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	F: GAACTGCCGTAACTCCCTTCTAT R: CATGACGAGGCTGGTGAACA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TGCCTGCTCTTGAGCTCGATCAAGCTATCATCAGCTACAGCTTCCGATCCCAACTGCAACTGTA
GCAGCGACAACTGCCATGGAGGCCCAGAACGTGGAGgttgctgccctggtgcagaagatcgcgg
ccctccacgccgacatcgccaagctgccgtcgctgagcccgtcccccgacgccaacgcgctgtt
caccagcc

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	TGCCTGCTCTTGAGCTCGATCAAGCTATCATCAGCTACAGCTTCCGATCCCAACTGCAACTGTA GCAGCGACAACTGCCATGGAGGCCCAGAACGTGGAGgttgctgccctggtgcagaagatcgcgg ccctccacgccgacatcgccaagctgccgtcgctgagcccgtcccccgacgccaacgcgctgtt caccagcc
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.292207507

Conservation Information
Conserved circRNAs	NA
PMCS	0.292207507

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							zma-miR166g-5p 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						regulating the response to N deficiency

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	zma-miR166g-5p
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	regulating the response to N deficiency

			
						 Other Information
					
						References
						Ma et al., 2021b

circRNA

Detail information of Zm00001d047656_circ_ag.1

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