Plant Circular RNA Database

Detail information of AT4G02220_circ_g.1

			
						 General Information
					
						CircRNA Name
						AT4G02220_circ_g.1
					
						ID in PlantcircBase
						ath_circ_029341
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr4: 976694-977363  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, PcircRNA_finder
					
						Parent gene
						
							AT4G02220						
					
						Parent gene annotation
						
							Putative zinc finger protein
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						8/3
					
						Tissues
						whole_plant/seedlings
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT4G02220.1:3

General Information
CircRNA Name	AT4G02220_circ_g.1
ID in PlantcircBase	ath_circ_029341
Alias	NA
Organism	Arabidpsis thaliana
Position	chr4: 976694-977363 JBrowse»
Reference genome	TAIR10.38
Type	ue-circRNA
Identification method	CIRCexplorer, KNIFE, PcircRNA_finder
Parent gene	AT4G02220
Parent gene annotation	Putative zinc finger protein
Parent gene strand	+
Alternative splicing	NA
Support reads	8/3
Tissues	whole_plant/seedlings
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT4G02220.1:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TTCATCGGACATTGTTTCTCTTCATGTGTCCATCTATGTCTTGTCTCCTTCGTGATCAACATGA
ACAATGGAAACGTGCCCCAGAGAAGGCTATGCGGAGtcttcatttcatcaaattcaacacagaa
gattcgatgagtagcttcaacggagactccatggatgatttcaaaggcctccgaataactcagc
ttgatgat

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 489
Transcript exons: 976694-976964,977063-977146,977230-977363
TCTTCATTTCATCAAATTCAACACAGAAGATTCGATGAGTAGCTTCAACGGAGACTCCATGGAT
GATTTCAAAGGCCTCCGAATAACTCAGCTTGATGATGATGACGATGATGAAACTGCGGTGGAAC
CTATAAATATGGATGAATTTGATGATGATGATGAGGAAGATGATGAAGATTATGAACCTGTGAT
GTTAGGTTTCGTTGAGAGTCCTAAATTCGCATGGTCAAATCTTCGTCAACTGTTCCCTAATTTA
GCTGGTGGTGTTCCGGCATGGTTGGATCCAGTTAATTTACCATCAGGGAAGTCAATTCTATGTG
ATCTATGTGAAGAACCTATGCAATTTGTACTTCAACTTTATGCTCCTTTAACAGACAAGGAATC
AGCTTTTCATCGGACATTGTTTCTCTTCATGTGTCCATCTATGTCTTGTCTCCTTCGTGATCAA
CATGAACAATGGAAACGTGCCCCAGAGAAGGCTATGCGGAG
						
						Genomic sequence
						
							TCTTCATTTCATCAAATTCAACACAGAAGATTCGATGAGTAGCTTCAACGGAGACTCCATGGAT
GATTTCAAAGGCCTCCGAATAACTCAGCTTGATGATGATGACGATGATGAAACTGCGGTGGAAC
CTATAAATATGGATGAATTTGATGATGATGATGAGGAAGATGATGAAGATTATGAACCTGTGAT
GTTAGGTTTCGTTGAGAGTCCTAAATTCGCATGGTCAAATCTTCGTCAACTGTTCCCTAATTTA
GCTGGTGGTGTTCCGGTACTAAGTTAAAACATCTTTCAATTTGTGCAATCAAAAACTGTTTCTT
CTTTATGTTCTTAGGTATTGATTCTTTGGGTTGATATTTGTCAATATAGGCATGGTTGGATCCA
GTTAATTTACCATCAGGGAAGTCAATTCTATGTGATCTATGTGAAGAACCTATGCAATTTGTAC
TTCAAGTAAGTTTCATACAAACTATTTTGTTGTTTCTTGCATGTTTTTTGTCGGTTTATTTATG
AAATTTGTTGTGGAAATTTGGCAGCTTTATGCTCCTTTAACAGACAAGGAATCAGCTTTTCATC
GGACATTGTTTCTCTTCATGTGTCCATCTATGTCTTGTCTCCTTCGTGATCAACATGAACAATG
GAAACGTGCCCCAGAGAAGGCTATGCGGAG

Sequences
Splice junction sequence	TTCATCGGACATTGTTTCTCTTCATGTGTCCATCTATGTCTTGTCTCCTTCGTGATCAACATGA ACAATGGAAACGTGCCCCAGAGAAGGCTATGCGGAGtcttcatttcatcaaattcaacacagaa gattcgatgagtagcttcaacggagactccatggatgatttcaaaggcctccgaataactcagc ttgatgat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 489 Transcript exons: 976694-976964,977063-977146,977230-977363 TCTTCATTTCATCAAATTCAACACAGAAGATTCGATGAGTAGCTTCAACGGAGACTCCATGGAT GATTTCAAAGGCCTCCGAATAACTCAGCTTGATGATGATGACGATGATGAAACTGCGGTGGAAC CTATAAATATGGATGAATTTGATGATGATGATGAGGAAGATGATGAAGATTATGAACCTGTGAT GTTAGGTTTCGTTGAGAGTCCTAAATTCGCATGGTCAAATCTTCGTCAACTGTTCCCTAATTTA GCTGGTGGTGTTCCGGCATGGTTGGATCCAGTTAATTTACCATCAGGGAAGTCAATTCTATGTG ATCTATGTGAAGAACCTATGCAATTTGTACTTCAACTTTATGCTCCTTTAACAGACAAGGAATC AGCTTTTCATCGGACATTGTTTCTCTTCATGTGTCCATCTATGTCTTGTCTCCTTCGTGATCAA CATGAACAATGGAAACGTGCCCCAGAGAAGGCTATGCGGAG
Genomic sequence	TCTTCATTTCATCAAATTCAACACAGAAGATTCGATGAGTAGCTTCAACGGAGACTCCATGGAT GATTTCAAAGGCCTCCGAATAACTCAGCTTGATGATGATGACGATGATGAAACTGCGGTGGAAC CTATAAATATGGATGAATTTGATGATGATGATGAGGAAGATGATGAAGATTATGAACCTGTGAT GTTAGGTTTCGTTGAGAGTCCTAAATTCGCATGGTCAAATCTTCGTCAACTGTTCCCTAATTTA GCTGGTGGTGTTCCGGTACTAAGTTAAAACATCTTTCAATTTGTGCAATCAAAAACTGTTTCTT CTTTATGTTCTTAGGTATTGATTCTTTGGGTTGATATTTGTCAATATAGGCATGGTTGGATCCA GTTAATTTACCATCAGGGAAGTCAATTCTATGTGATCTATGTGAAGAACCTATGCAATTTGTAC TTCAAGTAAGTTTCATACAAACTATTTTGTTGTTTCTTGCATGTTTTTTGTCGGTTTATTTATG AAATTTGTTGTGGAAATTTGGCAGCTTTATGCTCCTTTAACAGACAAGGAATCAGCTTTTCATC GGACATTGTTTCTCTTCATGTGTCCATCTATGTCTTGTCTCCTTCGTGATCAACATGAACAATG GAAACGTGCCCCAGAGAAGGCTATGCGGAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.329610126

Conservation Information
Conserved circRNAs	NA
PMCS	0.329610126

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							976728-977360(+)
						
						Potential amino acid sequence
						
							MSSFNGDSMDDFKGLRITQLDDDDDDETAVEPINMDEFDDDDEEDDEDYEPVMLGFVESPKFAW
SNLRQLFPNLAGGVPAWLDPVNLPSGKSILCDLCEEPMQFVLQLYAPLTDKESAFHRTLFLFMC
PSMSCLLRDQHEQWKRAPEKAMRSLHFIKFNTEDSMSSFNGDSMDDFKGLRITQLDDDDDDETA
VEPINMDEFDDDDEEDDEDYEPVMLGFVESPKFAWSNLRQLFPNLAGGVPAWLDPVNLPSGKSI
LCDLCEEPMQFVLQLYAPLTDKESAFHRTLFLFMCPSMSCLLRDQHEQWKRAPEKAMRSLHFIK
FNTEDSMSSFNGDSMDDFKGLRITQLDDDDDDETAVEPINMDEFDDDDEEDDEDYEPVMLGFVE
SPKFAWSNLRQLFPNLAGGVPAWLDPVNLPSGKSILCDLCEEPMQFVLQLYAPLTDKESAFHRT
LFLFMCPSMSCLLRDQHEQWKRAPEKAMRSLHFIKFNTEDSMSSFNGDSMDDFKGLRITQLDDD
DDDETAVEPINMDEFDDDDEEDDEDYEPVMLGFVESPKFAWSNLRQLFPNLAGGVPAWLDPVNL
PSGKSILCDLCEEPMQFVLQLYAPLTDKESAFHRTLFLFMCPSMSCLLRDQHEQWKRAPEKAMR
(+)
						
						Sponge-miRNAs
						
							ath-miR414 ath-miR5658 						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	976728-977360(+)
Potential amino acid sequence	MSSFNGDSMDDFKGLRITQLDDDDDDETAVEPINMDEFDDDDEEDDEDYEPVMLGFVESPKFAW SNLRQLFPNLAGGVPAWLDPVNLPSGKSILCDLCEEPMQFVLQLYAPLTDKESAFHRTLFLFMC PSMSCLLRDQHEQWKRAPEKAMRSLHFIKFNTEDSMSSFNGDSMDDFKGLRITQLDDDDDDETA VEPINMDEFDDDDEEDDEDYEPVMLGFVESPKFAWSNLRQLFPNLAGGVPAWLDPVNLPSGKSI LCDLCEEPMQFVLQLYAPLTDKESAFHRTLFLFMCPSMSCLLRDQHEQWKRAPEKAMRSLHFIK FNTEDSMSSFNGDSMDDFKGLRITQLDDDDDDETAVEPINMDEFDDDDEEDDEDYEPVMLGFVE SPKFAWSNLRQLFPNLAGGVPAWLDPVNLPSGKSILCDLCEEPMQFVLQLYAPLTDKESAFHRT LFLFMCPSMSCLLRDQHEQWKRAPEKAMRSLHFIKFNTEDSMSSFNGDSMDDFKGLRITQLDDD DDDETAVEPINMDEFDDDDEEDDEDYEPVMLGFVESPKFAWSNLRQLFPNLAGGVPAWLDPVNL PSGKSILCDLCEEPMQFVLQLYAPLTDKESAFHRTLFLFMCPSMSCLLRDQHEQWKRAPEKAMR (+)
Sponge-miRNAs	ath-miR414 ath-miR5658
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017;Pan et al., 2017

circRNA

Detail information of AT4G02220_circ_g.1

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