Plant Circular RNA Database

Detail information of AT3G23280_circ_g.4

			
						 General Information
					
						CircRNA Name
						AT3G23280_circ_g.4
					
						ID in PlantcircBase
						ath_circ_023335
					
						Alias
						At_ciR5461
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr3: 8322143-8323014  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							AT3G23280						
					
						Parent gene annotation
						
							Putative E3 ubiquitin-protein ligase XBAT35
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT3G23280_circ_g.1 AT3G23280_circ_g.2 AT3G23280_circ_g.3 AT3G23280_circ_g.5 AT3G23280_circ_g.6 						
					
						Support reads
						2
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT3G23280.2:5
AT3G23280.1:5

General Information
CircRNA Name	AT3G23280_circ_g.4
ID in PlantcircBase	ath_circ_023335
Alias	At_ciR5461
Organism	Arabidpsis thaliana
Position	chr3: 8322143-8323014 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	find_circ
Parent gene	AT3G23280
Parent gene annotation	Putative E3 ubiquitin-protein ligase XBAT35
Parent gene strand	+
Alternative splicing	AT3G23280_circ_g.1 AT3G23280_circ_g.2 AT3G23280_circ_g.3 AT3G23280_circ_g.5 AT3G23280_circ_g.6
Support reads	2
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT3G23280.2:5 AT3G23280.1:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GCACAGCCACGCACAGTGATGCCTTTGTGGAAAGCAAACTTGGAAGAACCAAAAGCGAAGCAGT
CCGATACTTCAGTGATGATCGTTGATAACTCCACAAgtcgtcatgctgggactcctctgcacca
tgctgcaaaaagaggtcttgagaacactgttaagttacttctttctcacggtggtaagtgtttg
tcttctca

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 447
Transcript exons: 8322143-8322223,8322320-8322408,8322510-8322601,8322723-
8322804,8322912-8323014
GTCGTCATGCTGGGACTCCTCTGCACCATGCTGCAAAAAGAGGTCTTGAGAACACTGTTAAGTT
ACTTCTTTCTCACGGTGCGAATCCACTTGTTCTTAATGATGATTGTCAGACACCACTTGAGGTC
GCTAGAGTCAAAGGGTTTAGCAATGTTGTACGTGCAATTGAGAAACACATCTGCTTGTTCTCGG
GTTGGATGCGTGAATTTTACGGCCCTACCTTTCTCGACTTATTTGCTCCGCAGCTTCTTTCAAG
AAGAGTATGGGTAGTCATTGTACCTACTGGTTCAAGAAACCCTACAAAGCCTTTCAAGTTGGAG
CTGGTTGTGTATGCTAGTCTGCAAGATGCACAGCCACGCACAGTGATGCCTTTGTGGAAAGCAA
ACTTGGAAGAACCAAAAGCGAAGCAGTCCGATACTTCAGTGATGATCGTTGATAACTCCACAA
						
						Genomic sequence
						
							GTCGTCATGCTGGGACTCCTCTGCACCATGCTGCAAAAAGAGGTCTTGAGAACACTGTTAAGTT
ACTTCTTTCTCACGGTGGTAAGTGTTTGTCTTCTCATACAACTCGATGAATGTGAGGTCAGTGA
ATACTTATGGTGTCTTTATCTCATATGGTGTAATCCCATTTTCTTGTAGCGAATCCACTTGTTC
TTAATGATGATTGTCAGACACCACTTGAGGTCGCTAGAGTCAAAGGGTTTAGCAATGTTGTACG
TGCAATTGAGGTATGTTTGTAATACCTGTTTGATCATTTATTCTAACAGACTTTTGAATGATAT
GACTACTTTGTGGATTAATAGTTTTTTTTTTATTGTGGATTCTGTAGAAACACATCTGCTTGTT
CTCGGGTTGGATGCGTGAATTTTACGGCCCTACCTTTCTCGACTTATTTGCTCCGCAGCTTCTT
TCAAGAAGAGTGTGAGTATCCATCCATTAAACTTCACCATTATATTTCTTTTTTACTCGTTTCT
TTCACTGAATCTGAGGAAAACATTTCTCTTCATGGCGTTGAATCTTCTTTAAACCTTACGATTT
GCAGATGGGTAGTCATTGTACCTACTGGTTCAAGAAACCCTACAAAGCCTTTCAAGTTGGAGCT
GGTTGTGTATGCTAGTCTGCAAGTATGATCATCATTAAACAATAATAAAATCATGACGTGTAAC
TTTTAATGCTTTTAAGCCTTGCATGTTTGGCTAATCTGTCTACAAAACTGATCTGATGTAATCA
GGATGCACAGCCACGCACAGTGATGCCTTTGTGGAAAGCAAACTTGGAAGAACCAAAAGCGAAG
CAGTCCGATACTTCAGTGATGATCGTTGATAACTCCACAA

Sequences
Splice junction sequence	GCACAGCCACGCACAGTGATGCCTTTGTGGAAAGCAAACTTGGAAGAACCAAAAGCGAAGCAGT CCGATACTTCAGTGATGATCGTTGATAACTCCACAAgtcgtcatgctgggactcctctgcacca tgctgcaaaaagaggtcttgagaacactgttaagttacttctttctcacggtggtaagtgtttg tcttctca
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 447 Transcript exons: 8322143-8322223,8322320-8322408,8322510-8322601,8322723- 8322804,8322912-8323014 GTCGTCATGCTGGGACTCCTCTGCACCATGCTGCAAAAAGAGGTCTTGAGAACACTGTTAAGTT ACTTCTTTCTCACGGTGCGAATCCACTTGTTCTTAATGATGATTGTCAGACACCACTTGAGGTC GCTAGAGTCAAAGGGTTTAGCAATGTTGTACGTGCAATTGAGAAACACATCTGCTTGTTCTCGG GTTGGATGCGTGAATTTTACGGCCCTACCTTTCTCGACTTATTTGCTCCGCAGCTTCTTTCAAG AAGAGTATGGGTAGTCATTGTACCTACTGGTTCAAGAAACCCTACAAAGCCTTTCAAGTTGGAG CTGGTTGTGTATGCTAGTCTGCAAGATGCACAGCCACGCACAGTGATGCCTTTGTGGAAAGCAA ACTTGGAAGAACCAAAAGCGAAGCAGTCCGATACTTCAGTGATGATCGTTGATAACTCCACAA
Genomic sequence	GTCGTCATGCTGGGACTCCTCTGCACCATGCTGCAAAAAGAGGTCTTGAGAACACTGTTAAGTT ACTTCTTTCTCACGGTGGTAAGTGTTTGTCTTCTCATACAACTCGATGAATGTGAGGTCAGTGA ATACTTATGGTGTCTTTATCTCATATGGTGTAATCCCATTTTCTTGTAGCGAATCCACTTGTTC TTAATGATGATTGTCAGACACCACTTGAGGTCGCTAGAGTCAAAGGGTTTAGCAATGTTGTACG TGCAATTGAGGTATGTTTGTAATACCTGTTTGATCATTTATTCTAACAGACTTTTGAATGATAT GACTACTTTGTGGATTAATAGTTTTTTTTTTATTGTGGATTCTGTAGAAACACATCTGCTTGTT CTCGGGTTGGATGCGTGAATTTTACGGCCCTACCTTTCTCGACTTATTTGCTCCGCAGCTTCTT TCAAGAAGAGTGTGAGTATCCATCCATTAAACTTCACCATTATATTTCTTTTTTACTCGTTTCT TTCACTGAATCTGAGGAAAACATTTCTCTTCATGGCGTTGAATCTTCTTTAAACCTTACGATTT GCAGATGGGTAGTCATTGTACCTACTGGTTCAAGAAACCCTACAAAGCCTTTCAAGTTGGAGCT GGTTGTGTATGCTAGTCTGCAAGTATGATCATCATTAAACAATAATAAAATCATGACGTGTAAC TTTTAATGCTTTTAAGCCTTGCATGTTTGGCTAATCTGTCTACAAAACTGATCTGATGTAATCA GGATGCACAGCCACGCACAGTGATGCCTTTGTGGAAAGCAAACTTGGAAGAACCAAAAGCGAAG CAGTCCGATACTTCAGTGATGATCGTTGATAACTCCACAA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.22693356

Conservation Information
Conserved circRNAs	NA
PMCS	0.22693356

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							8322537-8323011(+)
						
						Potential amino acid sequence
						
							MREFYGPTFLDLFAPQLLSRRVWVVIVPTGSRNPTKPFKLELVVYASLQDAQPRTVMPLWKANL
EEPKAKQSDTSVMIVDNSTSRHAGTPLHHAAKRGLENTVKLLLSHGANPLVLNDDCQTPLEVAR
VKGFSNVVRAIEKHICLFSGWMREFYGPTFLDLFAPQLLSRRVWVVIVPTGSRNPTKPFKLELV
VYASLQDAQPRTVMPLWKANLEEPKAKQSDTSVMIVDNSTSRHAGTPLHHAAKRGLENTVKLLL
SHGANPLVLNDDCQTPLEVARVKGFSNVVRAIEKHICLFSGWMREFYGPTFLDLFAPQLLSRRV
WVVIVPTGSRNPTKPFKLELVVYASLQDAQPRTVMPLWKANLEEPKAKQSDTSVMIVDNSTSRH
AGTPLHHAAKRGLENTVKLLLSHGANPLVLNDDCQTPLEVARVKGFSNVVRAIEKHICLFSGWM
REFYGPTFLDLFAPQLLSRRVWVVIVPTGSRNPTKPFKLELVVYASLQDAQPRTVMPLWKANLE
EPKAKQSDTSVMIVDNST(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	8322537-8323011(+)
Potential amino acid sequence	MREFYGPTFLDLFAPQLLSRRVWVVIVPTGSRNPTKPFKLELVVYASLQDAQPRTVMPLWKANL EEPKAKQSDTSVMIVDNSTSRHAGTPLHHAAKRGLENTVKLLLSHGANPLVLNDDCQTPLEVAR VKGFSNVVRAIEKHICLFSGWMREFYGPTFLDLFAPQLLSRRVWVVIVPTGSRNPTKPFKLELV VYASLQDAQPRTVMPLWKANLEEPKAKQSDTSVMIVDNSTSRHAGTPLHHAAKRGLENTVKLLL SHGANPLVLNDDCQTPLEVARVKGFSNVVRAIEKHICLFSGWMREFYGPTFLDLFAPQLLSRRV WVVIVPTGSRNPTKPFKLELVVYASLQDAQPRTVMPLWKANLEEPKAKQSDTSVMIVDNSTSRH AGTPLHHAAKRGLENTVKLLLSHGANPLVLNDDCQTPLEVARVKGFSNVVRAIEKHICLFSGWM REFYGPTFLDLFAPQLLSRRVWVVIVPTGSRNPTKPFKLELVVYASLQDAQPRTVMPLWKANLE EPKAKQSDTSVMIVDNST(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of AT3G23280_circ_g.4

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