Plant Circular RNA Database

Detail information of Os01g0188400_circ_g.8

			
						 General Information
					
						CircRNA Name
						Os01g0188400_circ_g.8
					
						ID in PlantcircBase
						osa_circ_000641
					
						Alias
						NA
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr1: 4742148-4742912  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							ui-circRNA							
									
						Identification method
						CIRCexplorer
					
						Parent gene
						
							Os01g0188525						
					
						Parent gene annotation
						
							Hypothetical gene. (Os01t0188525-00)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os01g0188400_circ_g.9 						
					
						Support reads
						1
					
						Tissues
						shoot
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os01t0188400-01:4
Os01t0188525-00:4
Os01t0188400-01:4

General Information
CircRNA Name	Os01g0188400_circ_g.8
ID in PlantcircBase	osa_circ_000641
Alias	NA
Organism	Oryza sativa
Position	chr1: 4742148-4742912 JBrowse»
Reference genome	IRGSP-1.0.38
Type	ui-circRNA
Identification method	CIRCexplorer
Parent gene	Os01g0188525
Parent gene annotation	Hypothetical gene. (Os01t0188525-00)
Parent gene strand	+
Alternative splicing	Os01g0188400_circ_g.9
Support reads	1
Tissues	shoot
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os01t0188400-01:4 Os01t0188525-00:4 Os01t0188400-01:4

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TGCCGGAGGAAGAAGGCCACGTAAGTAATGAGCATCCCGCTCCTTCTCACTAAAGGCAAGGCCC
TTGTTGTGATGTGGATCCCTCAAAAGGGTGTAACCActgacaacccaaatctccaagccccaaa
atgcgctcaccatcagtaacaacaataacttgaatgtttctctctggccagttccttagcacat
caagaacc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 454
Transcript exons: 4742148-4742253,4742350-4742501,4742634-4742708,4742792-
4742912
CTGACAACCCAAATCTCCAAGCCCCAAAATGCGCTCACCATCAGTAACAACAATAACTTGAATG
TTTCTCTCTGGCCAGTTCCTTAGCACATCAAGAACCTTTCCCTTGTCCTTCAAGCTGACATAGA
GACCCTGTGGTTGTCTAAATATGCTCCCATACTTCTGGCAGGCCTCACCTACTGTTGGTGTATA
CACCACAGGGAGAAGCTCCTCCACATTATCAATCAAAAGCTTGTAGAAGAGCCTCTCGTTCCTC
TCCTGGAGATCCATCATGGCCATGTAACGCTGCAAAGGTACACTGTACTGGCGAAGATTATGCA
TGATCTTCTTCACTTGAAGATCCTGAGACACAACTGCCGGAGGAAGAAGGCCACGTAAGTAATG
AGCATCCCGCTCCTTCTCACTAAAGGCAAGGCCCTTGTTGTGATGTGGATCCCTCAAAAGGGTG
TAACCA
						
						Genomic sequence
						
							CTGACAACCCAAATCTCCAAGCCCCAAAATGCGCTCACCATCAGTAACAACAATAACTTGAATG
TTTCTCTCTGGCCAGTTCCTTAGCACATCAAGAACCTTTCCCCTAGAATGCACTTCCAGTCAGT
CCAAATCGAATAGGTAGAGAGAATAAAATCAACACACTATTCTTGGATATTCAAGCAAAGCTAG
GCAAACATACTTGTCCTTCAAGCTGACATAGAGACCCTGTGGTTGTCTAAATATGCTCCCATAC
TTCTGGCAGGCCTCACCTACTGTTGGTGTATACACCACAGGGAGAAGCTCCTCCACATTATCAA
TCAAAAGCTTGTAGAAGAGCCTCTCGTTCCTCTCCTGATAAACACAAAGGTATTATTATCATAT
CTTCAACATGCTGTTCCTCCACATTGCCCAATGCCCAGTCATTTTTGTTAAATTGTAAATCATT
CAAGAAGTCATCAGGTAAAAAAGATTAATTTGGATTACCTGGAGATCCATCATGGCCATGTAAC
GCTGCAAAGGTACACTGTACTGGCGAAGATTATGCATGATCTTCTTCACCTGCACAAAACATTA
AGGAGGGCTTAAAATAAACGCCACAGAGTGATAAACAGGTTATAGTTGCTTATTGATGTAGCTC
GTACTTGAAGATCCTGAGACACAACTGCCGGAGGAAGAAGGCCACGTAAGTAATGAGCATCCCG
CTCCTTCTCACTAAAGGCAAGGCCCTTGTTGTGATGTGGATCCCTCAAAAGGGTGTAACCA

Sequences
Splice junction sequence	TGCCGGAGGAAGAAGGCCACGTAAGTAATGAGCATCCCGCTCCTTCTCACTAAAGGCAAGGCCC TTGTTGTGATGTGGATCCCTCAAAAGGGTGTAACCActgacaacccaaatctccaagccccaaa atgcgctcaccatcagtaacaacaataacttgaatgtttctctctggccagttccttagcacat caagaacc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 454 Transcript exons: 4742148-4742253,4742350-4742501,4742634-4742708,4742792- 4742912 CTGACAACCCAAATCTCCAAGCCCCAAAATGCGCTCACCATCAGTAACAACAATAACTTGAATG TTTCTCTCTGGCCAGTTCCTTAGCACATCAAGAACCTTTCCCTTGTCCTTCAAGCTGACATAGA GACCCTGTGGTTGTCTAAATATGCTCCCATACTTCTGGCAGGCCTCACCTACTGTTGGTGTATA CACCACAGGGAGAAGCTCCTCCACATTATCAATCAAAAGCTTGTAGAAGAGCCTCTCGTTCCTC TCCTGGAGATCCATCATGGCCATGTAACGCTGCAAAGGTACACTGTACTGGCGAAGATTATGCA TGATCTTCTTCACTTGAAGATCCTGAGACACAACTGCCGGAGGAAGAAGGCCACGTAAGTAATG AGCATCCCGCTCCTTCTCACTAAAGGCAAGGCCCTTGTTGTGATGTGGATCCCTCAAAAGGGTG TAACCA
Genomic sequence	CTGACAACCCAAATCTCCAAGCCCCAAAATGCGCTCACCATCAGTAACAACAATAACTTGAATG TTTCTCTCTGGCCAGTTCCTTAGCACATCAAGAACCTTTCCCCTAGAATGCACTTCCAGTCAGT CCAAATCGAATAGGTAGAGAGAATAAAATCAACACACTATTCTTGGATATTCAAGCAAAGCTAG GCAAACATACTTGTCCTTCAAGCTGACATAGAGACCCTGTGGTTGTCTAAATATGCTCCCATAC TTCTGGCAGGCCTCACCTACTGTTGGTGTATACACCACAGGGAGAAGCTCCTCCACATTATCAA TCAAAAGCTTGTAGAAGAGCCTCTCGTTCCTCTCCTGATAAACACAAAGGTATTATTATCATAT CTTCAACATGCTGTTCCTCCACATTGCCCAATGCCCAGTCATTTTTGTTAAATTGTAAATCATT CAAGAAGTCATCAGGTAAAAAAGATTAATTTGGATTACCTGGAGATCCATCATGGCCATGTAAC GCTGCAAAGGTACACTGTACTGGCGAAGATTATGCATGATCTTCTTCACCTGCACAAAACATTA AGGAGGGCTTAAAATAAACGCCACAGAGTGATAAACAGGTTATAGTTGCTTATTGATGTAGCTC GTACTTGAAGATCCTGAGACACAACTGCCGGAGGAAGAAGGCCACGTAAGTAATGAGCATCCCG CTCCTTCTCACTAAAGGCAAGGCCCTTGTTGTGATGTGGATCCCTCAAAAGGGTGTAACCA

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_011302 						
					
						PMCS
						0.380156307

Conservation Information
Conserved circRNAs	osi_circ_011302
PMCS	0.380156307

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							4742841-4742191(+)
4742698-4742864(-)
4742190-4742864(-)
						
						Potential amino acid sequence
						
							MSIPLLLTKGKALVVMWIPQKGVTTDNPNLQAPKCAHHQ*(+)
MHNLRQYSVPLQRYMAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRQPQG
LYVSLKDKGKVLDVLRNWPERNIQVIVVTDGERILGLGDLGCQWLHPFEGSTSQQGPCL*(-)
MVSAFWGLEIWVVSGYTLLRDPHHNKGLAFSEKERDAHYLRGLLPPAVVSQDLQVKKIMHNLRQ
YSVPLQRYMAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRQPQGLYVSLK
DKGKVLDVLRNWPERNIQVIVVTDGERILGLGDLGCQWLHPFEGSTSQQGPCL*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	4742841-4742191(+) 4742698-4742864(-) 4742190-4742864(-)
Potential amino acid sequence	MSIPLLLTKGKALVVMWIPQKGVTTDNPNLQAPKCAHHQ(+) MHNLRQYSVPLQRYMAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRQPQG LYVSLKDKGKVLDVLRNWPERNIQVIVVTDGERILGLGDLGCQWLHPFEGSTSQQGPCL(-) MVSAFWGLEIWVVSGYTLLRDPHHNKGLAFSEKERDAHYLRGLLPPAVVSQDLQVKKIMHNLRQ YSVPLQRYMAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRQPQGLYVSLK DKGKVLDVLRNWPERNIQVIVVTDGERILGLGDLGCQWLHPFEGSTSQQGPCL*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of Os01g0188400_circ_g.8

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