Plant Circular RNA Database

Detail information of Zm00001d035156_circ_g.3

			
						 General Information
					
						CircRNA Name
						Zm00001d035156_circ_g.3
					
						ID in PlantcircBase
						zma_circ_002197
					
						Alias
						circ1026
					
						Organism
						
							Zea mays						
					
						Position
						
							chr6: 7238431-7238658  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Zm00001d035156						
					
						Parent gene annotation
						
							Glyceraldehyde-3-phosphate dehydrogenase 2, cytosolic  
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Zm00001d035156_circ_g.1 Zm00001d035156_circ_g.2 Zm00001d035156_circ_g.4 						
					
						Support reads
						2
					
						Tissues
						seedling leaves
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-CA							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d035156_T001:1
Zm00001d035156_T007:2
Zm00001d035156_T003:2
Zm00001d035156_T002:2
Zm00001d035156_T008:1
Zm00001d035156_T005:2
Zm00001d035156_T006:2
Zm00001d035156_T004:2

General Information
CircRNA Name	Zm00001d035156_circ_g.3
ID in PlantcircBase	zma_circ_002197
Alias	circ1026
Organism	Zea mays
Position	chr6: 7238431-7238658 JBrowse»
Reference genome	AGPv4.38
Type	ue-circRNA
Identification method	CIRI
Parent gene	Zm00001d035156
Parent gene annotation	Glyceraldehyde-3-phosphate dehydrogenase 2, cytosolic
Parent gene strand	+
Alternative splicing	Zm00001d035156_circ_g.1 Zm00001d035156_circ_g.2 Zm00001d035156_circ_g.4
Support reads	2
Tissues	seedling leaves
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-CA The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d035156_T001:1 Zm00001d035156_T007:2 Zm00001d035156_T003:2 Zm00001d035156_T002:2 Zm00001d035156_T008:1 Zm00001d035156_T005:2 Zm00001d035156_T006:2 Zm00001d035156_T004:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TTTCGGAAGGATCGGCAGGCTCGTGGCCAGGGTCGCCCTGCAGAGCGAGGATGTCGAGCTCGTC
GCCGTCAACGACCCCTTCATCACCACGGATTACATGggcaagatcaagatcggaatcaacggtg
agtctgtacccccaacacaaactcgttcttcctgctcggttcgttgggtctggactctggagtg
atctgagt

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GGCAAGATCAAGATCGGAATCAACGGTGAGTCTGTACCCCCAACACAAACTCGTTCTTCCTGCT
CGGTTCGTTGGGTCTGGACTCTGGAGTGATCTGAGTGGGGTCTCTGCGCGCCTGCGTGCTCAGG
TTTCGGAAGGATCGGCAGGCTCGTGGCCAGGGTCGCCCTGCAGAGCGAGGATGTCGAGCTCGTC
GCCGTCAACGACCCCTTCATCACCACGGATTACATG

Sequences
Splice junction sequence	TTTCGGAAGGATCGGCAGGCTCGTGGCCAGGGTCGCCCTGCAGAGCGAGGATGTCGAGCTCGTC GCCGTCAACGACCCCTTCATCACCACGGATTACATGggcaagatcaagatcggaatcaacggtg agtctgtacccccaacacaaactcgttcttcctgctcggttcgttgggtctggactctggagtg atctgagt
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GGCAAGATCAAGATCGGAATCAACGGTGAGTCTGTACCCCCAACACAAACTCGTTCTTCCTGCT CGGTTCGTTGGGTCTGGACTCTGGAGTGATCTGAGTGGGGTCTCTGCGCGCCTGCGTGCTCAGG TTTCGGAAGGATCGGCAGGCTCGTGGCCAGGGTCGCCCTGCAGAGCGAGGATGTCGAGCTCGTC GCCGTCAACGACCCCTTCATCACCACGGATTACATG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.462068604

Conservation Information
Conserved circRNAs	NA
PMCS	0.462068604

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							7238657-7238655(+)
7238610-7238655(+)
7238644-7238654(-)
						
						Potential amino acid sequence
						
							MGKIKIGINGFGRIGRLVARVALQSEDVELVAVNDPFITTDYMGKIKIGINGFGRIGRLVARVA
LQSEDVELVAVNDPFITTDYMGKIKIGINGFGRIGRLVARVALQSEDVELVAVNDPFITTDYM(
+)
MSSSSPSTTPSSPRITWARSRSESTVSEGSAGSWPGSPCRARMSSSSPSTTPSSPRITWARSRS
ESTVSEGSAGSWPGSPCRARMSSSSPSTTPSSPRITWARSRSESTVSEGSAGSWPGSPCRARMS
SSSPSTTPSSPRIT(+)
MKGSLTATSSTSSLCRATLATSLPILPKPLIPILILPM*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	7238657-7238655(+) 7238610-7238655(+) 7238644-7238654(-)
Potential amino acid sequence	MGKIKIGINGFGRIGRLVARVALQSEDVELVAVNDPFITTDYMGKIKIGINGFGRIGRLVARVA LQSEDVELVAVNDPFITTDYMGKIKIGINGFGRIGRLVARVALQSEDVELVAVNDPFITTDYM( +) MSSSSPSTTPSSPRITWARSRSESTVSEGSAGSWPGSPCRARMSSSSPSTTPSSPRITWARSRS ESTVSEGSAGSWPGSPCRARMSSSSPSTTPSSPRITWARSRSESTVSEGSAGSWPGSPCRARMS SSSPSTTPSSPRIT(+) MKGSLTATSSTSSLCRATLATSLPILPKPLIPILILPM*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chen et al., 2017b

circRNA

Detail information of Zm00001d035156_circ_g.3

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