Plant Circular RNA Database

Detail information of Csa4G050840_circ_g.1

			
						 General Information
					
						CircRNA Name
						Csa4G050840_circ_g.1
					
						ID in PlantcircBase
						csa_circ_002365
					
						Alias
						Chr4:4149473_4150337
					
						Organism
						
							Cucumis sativus						
					
						Position
						
							chrChr4: 4149473-4150337  JBrowse»						
					
						Reference genome
						
							Cucumis sativus v1.5						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI2
					
						Parent gene
						
							Csa4G050840						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						-
					
						Alternative splicing
						
							NA						
					
						Support reads
						NA
					
						Tissues
						leaf,root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Csa4G050840.1:3

General Information
CircRNA Name	Csa4G050840_circ_g.1
ID in PlantcircBase	csa_circ_002365
Alias	Chr4:4149473_4150337
Organism	Cucumis sativus
Position	chrChr4: 4149473-4150337 JBrowse»
Reference genome	Cucumis sativus v1.5
Type	e-circRNA
Identification method	CIRI2
Parent gene	Csa4G050840
Parent gene annotation	NA
Parent gene strand	-
Alternative splicing	NA
Support reads	NA
Tissues	leaf,root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Csa4G050840.1:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GGATTAACTTAATAACAACCTCATCAGTAGTCAGAAAATTTGGCCTGACTATATGCCTTCGTGA
ACAAGTAAAAGTTCTTCGAAGACCAGCATGCATTCCcatggcaaccagctccgcaacatcaatg
cttagtggatcaaactccgagttgatcttttcaccaacatttgtgagaatatcctgcagtctgg
atattgac

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 525
Transcript exons: 4149473-4149803,4150031-4150100,4150214-4150337
CATGGCAACCAGCTCCGCAACATCAATGCTTAGTGGATCAAACTCCGAGTTGATCTTTTCACCA
ACATTTGTGAGAATATCCTGCAGTCTGGATATTGACTCACCAGAACGTTTAGCAAGCTCAACGA
TTGTCATGCCATCAAATATCTCAATTGTTTTATCAGAAACAGATCCAATGGAGTGTTTCGGTTT
TGGAGGAACATATGGAGCTTCAACTGGTGGCTGATTCCTGCCATCCTTTCTTACAAACTTTTCT
TTCTTTTGAGTCTTCAAACCAAATTCCTTATCATGTCCTCTTCCAGCCAATAATTCTGCACTTG
AATGGAAACACCTTCTTGGAGCTTCTATTGTAGAAGCCACATAAAAATCAGACCCATGATATGC
TGAACCACAAGGGACGTCTGGTATAAATCTTGAAGAAGCAGGGATTAACTTAATAACAACCTCA
TCAGTAGTCAGAAAATTTGGCCTGACTATATGCCTTCGTGAACAAGTAAAAGTTCTTCGAAGAC
CAGCATGCATTCC
						
						Genomic sequence
						
							CATGGCAACCAGCTCCGCAACATCAATGCTTAGTGGATCAAACTCCGAGTTGATCTTTTCACCA
ACATTTGTGAGAATATCCTGCAGTCTGGATATTGACTCACCAGAACGTTTAGCAAGCTCAACGA
TTGTCATGCCATCAAATATCTCAATTGTTTTATCAGAAACAGATCCAATGGAGTGTTTCGGTTT
TGGAGGAACATATGGAGCTTCAACTGGTGGCTGATTCCTGCCATCCTTTCTTACAAACTTTTCT
TTCTTTTGAGTCTTCAAACCAAATTCCTTATCATGTCCTCTTCCAGCCAATAATTCTGCACTTG
AATGGAAACACCTAAACAATTTTTTTTAAAAAAGAAAAAAACATAAGATGCACTGCAAACCAAA
GTTCAAATCTTTTCCTTGTAATTTCATATACATTAATCAATCCAGGATCCTCTCCTTATCACTG
ATCAATATTTAATGCCAAATTACATTGCTAACTTCATTAATAACAGCAATAGTTGGATGATATT
AGTAGACAACAGAAACGACTGTATAGTAATGAAATGGATGCATTACCTTCTTGGAGCTTCTATT
GTAGAAGCCACATAAAAATCAGACCCATGATATGCTGAACCACAAGGGACGTCTGCACCAATCA
TTTGTGTTGCAAAAATGGCCACATAAACACTAACGTTTGTAATCAAATTCAAGAACTTCATATG
ATGTAAGGCAAAAAAGATGCCTACAATTAGGTGTTACCTGGTATAAATCTTGAAGAAGCAGGGA
TTAACTTAATAACAACCTCATCAGTAGTCAGAAAATTTGGCCTGACTATATGCCTTCGTGAACA
AGTAAAAGTTCTTCGAAGACCAGCATGCATTCC

Sequences
Splice junction sequence	GGATTAACTTAATAACAACCTCATCAGTAGTCAGAAAATTTGGCCTGACTATATGCCTTCGTGA ACAAGTAAAAGTTCTTCGAAGACCAGCATGCATTCCcatggcaaccagctccgcaacatcaatg cttagtggatcaaactccgagttgatcttttcaccaacatttgtgagaatatcctgcagtctgg atattgac
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 525 Transcript exons: 4149473-4149803,4150031-4150100,4150214-4150337 CATGGCAACCAGCTCCGCAACATCAATGCTTAGTGGATCAAACTCCGAGTTGATCTTTTCACCA ACATTTGTGAGAATATCCTGCAGTCTGGATATTGACTCACCAGAACGTTTAGCAAGCTCAACGA TTGTCATGCCATCAAATATCTCAATTGTTTTATCAGAAACAGATCCAATGGAGTGTTTCGGTTT TGGAGGAACATATGGAGCTTCAACTGGTGGCTGATTCCTGCCATCCTTTCTTACAAACTTTTCT TTCTTTTGAGTCTTCAAACCAAATTCCTTATCATGTCCTCTTCCAGCCAATAATTCTGCACTTG AATGGAAACACCTTCTTGGAGCTTCTATTGTAGAAGCCACATAAAAATCAGACCCATGATATGC TGAACCACAAGGGACGTCTGGTATAAATCTTGAAGAAGCAGGGATTAACTTAATAACAACCTCA TCAGTAGTCAGAAAATTTGGCCTGACTATATGCCTTCGTGAACAAGTAAAAGTTCTTCGAAGAC CAGCATGCATTCC
Genomic sequence	CATGGCAACCAGCTCCGCAACATCAATGCTTAGTGGATCAAACTCCGAGTTGATCTTTTCACCA ACATTTGTGAGAATATCCTGCAGTCTGGATATTGACTCACCAGAACGTTTAGCAAGCTCAACGA TTGTCATGCCATCAAATATCTCAATTGTTTTATCAGAAACAGATCCAATGGAGTGTTTCGGTTT TGGAGGAACATATGGAGCTTCAACTGGTGGCTGATTCCTGCCATCCTTTCTTACAAACTTTTCT TTCTTTTGAGTCTTCAAACCAAATTCCTTATCATGTCCTCTTCCAGCCAATAATTCTGCACTTG AATGGAAACACCTAAACAATTTTTTTTAAAAAAGAAAAAAACATAAGATGCACTGCAAACCAAA GTTCAAATCTTTTCCTTGTAATTTCATATACATTAATCAATCCAGGATCCTCTCCTTATCACTG ATCAATATTTAATGCCAAATTACATTGCTAACTTCATTAATAACAGCAATAGTTGGATGATATT AGTAGACAACAGAAACGACTGTATAGTAATGAAATGGATGCATTACCTTCTTGGAGCTTCTATT GTAGAAGCCACATAAAAATCAGACCCATGATATGCTGAACCACAAGGGACGTCTGCACCAATCA TTTGTGTTGCAAAAATGGCCACATAAACACTAACGTTTGTAATCAAATTCAAGAACTTCATATG ATGTAAGGCAAAAAAGATGCCTACAATTAGGTGTTACCTGGTATAAATCTTGAAGAAGCAGGGA TTAACTTAATAACAACCTCATCAGTAGTCAGAAAATTTGGCCTGACTATATGCCTTCGTGAACA AGTAAAAGTTCTTCGAAGACCAGCATGCATTCC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.48843474

Conservation Information
Conserved circRNAs	NA
PMCS	0.48843474

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							4150336-4149475(-)
4149497-4150288(-)
						
						Potential amino acid sequence
						
							MHAGLRRTFTCSRRHIVRPNFLTTDEVVIKLIPASSRFIPDVPCGSAYHGSDFYVASTIEAPRR
CFHSSAELLAGRGHDKEFGLKTQKKEKFVRKDGRNQPPVEAPYVPPKPKHSIGSVSDKTIEIFD
GMTIVELAKRSGESISRLQDILTNVGEKINSEFDPLSIDVAELVAMGMHAGLRRTFTCSRRHIV
RPNFLTTDEVVIKLIPASSRFIPDVPCGSAYHGSDFYVASTIEAPRRCFHSSAELLAGRGHDKE
FGLKTQKKEKFVRKDGRNQPPVEAPYVPPKPKHSIGSVSDKTIEIFDGMTIVELAKRSGESISR
LQDILTNVGEKINSEFDPLSIDVAELVAMGMHAGLRRTFTCSRRHIVRPNFLTTDEVVIKLIPA
SSRFIPDVPCGSAYHGSDFYVASTIEAPRRCFHSSAELLAGRGHDKEFGLKTQKKEKFVRKDGR
NQPPVEAPYVPPKPKHSIGSVSDKTIEIFDGMTIVELAKRSGESISRLQDILTNVGEKINSEFD
PLSIDVAELVAMGMHAGLRRTFTCSRRHIVRPNFLTTDEVVIKLIPASSRFIPDVPCGSAYHGS
DFYVASTIEAPRRCFHSSAELLAGRGHDKEFGLKTQKKEKFVRKDGRNQPPVEAPYVPPKPKHS
IGSVSDKTIEIFDGMTIVELAKRSGESISRLQDILTNVGEKINSEFDPLSIDVAELVAM
MLRSWLPWECMLVFEELLLVHEGI*
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	4150336-4149475(-) 4149497-4150288(-)
Potential amino acid sequence	MHAGLRRTFTCSRRHIVRPNFLTTDEVVIKLIPASSRFIPDVPCGSAYHGSDFYVASTIEAPRR CFHSSAELLAGRGHDKEFGLKTQKKEKFVRKDGRNQPPVEAPYVPPKPKHSIGSVSDKTIEIFD GMTIVELAKRSGESISRLQDILTNVGEKINSEFDPLSIDVAELVAMGMHAGLRRTFTCSRRHIV RPNFLTTDEVVIKLIPASSRFIPDVPCGSAYHGSDFYVASTIEAPRRCFHSSAELLAGRGHDKE FGLKTQKKEKFVRKDGRNQPPVEAPYVPPKPKHSIGSVSDKTIEIFDGMTIVELAKRSGESISR LQDILTNVGEKINSEFDPLSIDVAELVAMGMHAGLRRTFTCSRRHIVRPNFLTTDEVVIKLIPA SSRFIPDVPCGSAYHGSDFYVASTIEAPRRCFHSSAELLAGRGHDKEFGLKTQKKEKFVRKDGR NQPPVEAPYVPPKPKHSIGSVSDKTIEIFDGMTIVELAKRSGESISRLQDILTNVGEKINSEFD PLSIDVAELVAMGMHAGLRRTFTCSRRHIVRPNFLTTDEVVIKLIPASSRFIPDVPCGSAYHGS DFYVASTIEAPRRCFHSSAELLAGRGHDKEFGLKTQKKEKFVRKDGRNQPPVEAPYVPPKPKHS IGSVSDKTIEIFDGMTIVELAKRSGESISRLQDILTNVGEKINSEFDPLSIDVAELVAM MLRSWLPWECMLVFEELLLVHEGI*
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhu et al., 2019

circRNA

Detail information of Csa4G050840_circ_g.1

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