Plant Circular RNA Database

Detail information of Zm00001d045747_circ_g.3

			
						 General Information
					
						CircRNA Name
						Zm00001d045747_circ_g.3
					
						ID in PlantcircBase
						zma_circ_009922
					
						Alias
						Zm09circ00029, zma_circ_0002940, GRMZM5G883025_C1
					
						Organism
						
							Zea mays						
					
						Position
						
							chr9: 36906444-36907044  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI2, find_circ
					
						Parent gene
						
							Zm00001d045747						
					
						Parent gene annotation
						
							DNA topoisomerase type IA core
							
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						NA
					
						Tissues
						leaf, endosperm, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d045747_T002:3
Zm00001d045747_T003:2
Zm00001d045747_T001:3
Zm00001d045747_T004:3

General Information
CircRNA Name	Zm00001d045747_circ_g.3
ID in PlantcircBase	zma_circ_009922
Alias	Zm09circ00029, zma_circ_0002940, GRMZM5G883025_C1
Organism	Zea mays
Position	chr9: 36906444-36907044 JBrowse»
Reference genome	AGPv4.38
Type	e-circRNA
Identification method	CIRI2, find_circ
Parent gene	Zm00001d045747
Parent gene annotation	DNA topoisomerase type IA core
Parent gene strand	+
Alternative splicing	NA
Support reads	NA
Tissues	leaf, endosperm, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d045747_T002:3 Zm00001d045747_T003:2 Zm00001d045747_T001:3 Zm00001d045747_T004:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CTCATTATCACAACAAGAAGATGAAACCGAACCCACCGAAGAATGTCAAAAAACTTTTACACCC
AGATTACTTGGTGTCATGCCTGATTCTGATGAAAAGgtagagagaatgctcgaagaaaaatttg
gtcctatcctcttccctaatgttgacacagacaatagaatttgccctaggtaatgcacgacaaa
catacttt

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 273
Transcript exons: 36906444-36906520,36906692-36906775,36906933-36907044
GTAGAGAGAATGCTCGAAGAAAAATTTGGTCCTATCCTCTTCCCTAATGTTGACACAGACAATA
GAATTTGCCCTAGTTGTTCTGAAGGAACCTTGAGATTCAAAGTTAGTAGGTATGGTGAAGGCTA
TTTTATAGGCTGTGATCGACATCCAAAATGCAAGTACATTGCTCGCTCATTATCACAACAAGAA
GATGAAACCGAACCCACCGAAGAATGTCAAAAAACTTTTACACCCAGATTACTTGGTGTCATGC
CTGATTCTGATGAAAAG
						
						Genomic sequence
						
							GTAGAGAGAATGCTCGAAGAAAAATTTGGTCCTATCCTCTTCCCTAATGTTGACACAGACAATA
GAATTTGCCCTAGGTAATGCACGACAAACATACTTTTAAGGTATCTTTCGTGGAAAGTACATAT
TGAATGTTGAAATCAAGATATTTGCAATATTCATTCAGGTTACCCTACAATATGTTCATACTGT
GATGCATTCACACACGTAGTTCTTGACATCTCTTTTTGTGTTTGGAATACATGTAGTTGTTCTG
AAGGAACCTTGAGATTCAAAGTTAGTAGGTATGGTGAAGGCTATTTTATAGGCTGTGATCGACA
TCCAAAATGCAAGTATGACCCTATTATCCCTCAGTATAATTGGAATGCCTTGAAGTTTAAACTC
TACTATATTCTTCATTGTCACCTATTTTGTATTAATGAGTTCCAGGGTTATTTTTTGATTGTTG
ATGATTATCATCCTACCTAATCACTGATCTAATTACTGTAGGTACATTGCTCGCTCATTATCAC
AACAAGAAGATGAAACCGAACCCACCGAAGAATGTCAAAAAACTTTTACACCCAGATTACTTGG
TGTCATGCCTGATTCTGATGAAAAG

Sequences
Splice junction sequence	CTCATTATCACAACAAGAAGATGAAACCGAACCCACCGAAGAATGTCAAAAAACTTTTACACCC AGATTACTTGGTGTCATGCCTGATTCTGATGAAAAGgtagagagaatgctcgaagaaaaatttg gtcctatcctcttccctaatgttgacacagacaatagaatttgccctaggtaatgcacgacaaa catacttt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 273 Transcript exons: 36906444-36906520,36906692-36906775,36906933-36907044 GTAGAGAGAATGCTCGAAGAAAAATTTGGTCCTATCCTCTTCCCTAATGTTGACACAGACAATA GAATTTGCCCTAGTTGTTCTGAAGGAACCTTGAGATTCAAAGTTAGTAGGTATGGTGAAGGCTA TTTTATAGGCTGTGATCGACATCCAAAATGCAAGTACATTGCTCGCTCATTATCACAACAAGAA GATGAAACCGAACCCACCGAAGAATGTCAAAAAACTTTTACACCCAGATTACTTGGTGTCATGC CTGATTCTGATGAAAAG
Genomic sequence	GTAGAGAGAATGCTCGAAGAAAAATTTGGTCCTATCCTCTTCCCTAATGTTGACACAGACAATA GAATTTGCCCTAGGTAATGCACGACAAACATACTTTTAAGGTATCTTTCGTGGAAAGTACATAT TGAATGTTGAAATCAAGATATTTGCAATATTCATTCAGGTTACCCTACAATATGTTCATACTGT GATGCATTCACACACGTAGTTCTTGACATCTCTTTTTGTGTTTGGAATACATGTAGTTGTTCTG AAGGAACCTTGAGATTCAAAGTTAGTAGGTATGGTGAAGGCTATTTTATAGGCTGTGATCGACA TCCAAAATGCAAGTATGACCCTATTATCCCTCAGTATAATTGGAATGCCTTGAAGTTTAAACTC TACTATATTCTTCATTGTCACCTATTTTGTATTAATGAGTTCCAGGGTTATTTTTTGATTGTTG ATGATTATCATCCTACCTAATCACTGATCTAATTACTGTAGGTACATTGCTCGCTCATTATCAC AACAAGAAGATGAAACCGAACCCACCGAAGAATGTCAAAAAACTTTTACACCCAGATTACTTGG TGTCATGCCTGATTCTGATGAAAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.19271391

Conservation Information
Conserved circRNAs	NA
PMCS	0.19271391

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							36906454-36907041(+)
36906966-36906444(+)
36906766-36907014(-)
						
						Potential amino acid sequence
						
							MLEEKFGPILFPNVDTDNRICPSCSEGTLRFKVSRYGEGYFIGCDRHPKCKYIARSLSQQEDET
EPTEECQKTFTPRLLGVMPDSDEKVERMLEEKFGPILFPNVDTDNRICPSCSEGTLRFKVSRYG
EGYFIGCDRHPKCKYIARSLSQQEDETEPTEECQKTFTPRLLGVMPDSDEKVERMLEEKFGPIL
FPNVDTDNRICPSCSEGTLRFKVSRYGEGYFIGCDRHPKCKYIARSLSQQEDETEPTEECQKTF
TPRLLGVMPDSDEKVERMLEEKFGPILFPNVDTDNRICPSCSEGTLRFKVSRYGEGYFIGCDRH
PKCKYIARSLSQQEDETEPTEECQKTFTPRLLGVMPDSDEK(+)
MKPNPPKNVKKLLHPDYLVSCLILMKR*(+)
MSITAYKIAFTIPTNFESQGSFRTTRANSIVCVNIREEDRTKFFFEHSLYLFIRIRHDTK*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	36906454-36907041(+) 36906966-36906444(+) 36906766-36907014(-)
Potential amino acid sequence	MLEEKFGPILFPNVDTDNRICPSCSEGTLRFKVSRYGEGYFIGCDRHPKCKYIARSLSQQEDET EPTEECQKTFTPRLLGVMPDSDEKVERMLEEKFGPILFPNVDTDNRICPSCSEGTLRFKVSRYG EGYFIGCDRHPKCKYIARSLSQQEDETEPTEECQKTFTPRLLGVMPDSDEKVERMLEEKFGPIL FPNVDTDNRICPSCSEGTLRFKVSRYGEGYFIGCDRHPKCKYIARSLSQQEDETEPTEECQKTF TPRLLGVMPDSDEKVERMLEEKFGPILFPNVDTDNRICPSCSEGTLRFKVSRYGEGYFIGCDRH PKCKYIARSLSQQEDETEPTEECQKTFTPRLLGVMPDSDEK(+) MKPNPPKNVKKLLHPDYLVSCLILMKR(+) MSITAYKIAFTIPTNFESQGSFRTTRANSIVCVNIREEDRTKFFFEHSLYLFIRIRHDTK(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Han et al., 2020; Ma et al., 2021b;Zhang et al., 2019

circRNA

Detail information of Zm00001d045747_circ_g.3

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