Plant Circular RNA Database

Detail information of AT5G15540_circ_g.2

			
						 General Information
					
						CircRNA Name
						AT5G15540_circ_g.2
					
						ID in PlantcircBase
						ath_circ_038419
					
						Alias
						At_ciR2897
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 5050936-5051721  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ
					
						Parent gene
						
							AT5G15540						
					
						Parent gene annotation
						
							Sister chromatid cohesion protein SCC2
							
						Parent gene strand
						-
					
						Alternative splicing
						
							AT5G15540_circ_g.3 AT5G15540_circ_g.4 						
					
						Support reads
						3
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G15540.2:3
AT5G15540.1:3

General Information
CircRNA Name	AT5G15540_circ_g.2
ID in PlantcircBase	ath_circ_038419
Alias	At_ciR2897
Organism	Arabidpsis thaliana
Position	chr5: 5050936-5051721 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	find_circ
Parent gene	AT5G15540
Parent gene annotation	Sister chromatid cohesion protein SCC2
Parent gene strand	-
Alternative splicing	AT5G15540_circ_g.3 AT5G15540_circ_g.4
Support reads	3
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G15540.2:3 AT5G15540.1:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CCGGTGTACACAGCCCAGGATCCACCAGACAGAAAGCATGCAAAACAAGTACATAAGGAAGTAC
TTGCACCTCTCCTTCTTCACGGCTCATTTCTTCTACctgtgtgttatcggaaccttgggcctcc
agccgcttaaaaaagaactgaagaagatgctcaactatgctaacacctttccccgccaatttac
tcacagaa

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 432
Transcript exons: 5050936-5051044,5051212-5051381,5051569-5051721
CTGTGTGTTATCGGAACCTTGGGCCTCCAGCCGCTTAAAAAAGAACTGAAGAAGATGCTCAACT
ATGCTAACACCTTTCCCCGCCAATTTACTCACAGAACAAAGACACCTAACGCAAGCATGCACAA
CAGTTAGAAACGAATGCCGAACAATCATATGCTTTAGATCCTGTTCCAGATCTTCTGTGACACT
TAGAGGCAGCTTGCGGATCAAGGGTAGGACAGAATCGATAATGAAAATAATACTCTCCAGTAAC
TGTGCTCCTGTTCTACTATCAGCCTGACTCTTCAGATATGGCTGTAGGGTGATGACAAACTTAG
TAGGGTCTGAAGCCGGTGTACACAGCCCAGGATCCACCAGACAGAAAGCATGCAAAACAAGTAC
ATAAGGAAGTACTTGCACCTCTCCTTCTTCACGGCTCATTTCTTCTAC
						
						Genomic sequence
						
							CTGTGTGTTATCGGAACCTTGGGCCTCCAGCCGCTTAAAAAAGAACTGAAGAAGATGCTCAACT
ATGCTAACACCTTTCCCCGCCAATTTACTCACAGAACAAAGACACCTGAGAATTCAAAAGGTTG
CATTAGTAGTCTCCTGAAAAGGATGAGTGATTTTACTCGACTGCATCTTCTATGAAGACATGCA
ATCGTCCCAAAAACAATGAACATATGTGAAAGAGAATACGATGAGTAGAGCCAGGAGTGATGGT
AGTTGAAATATAGAGGGTACCTAACGCAAGCATGCACAACAGTTAGAAACGAATGCCGAACAAT
CATATGCTTTAGATCCTGTTCCAGATCTTCTGTGACACTTAGAGGCAGCTTGCGGATCAAGGGT
AGGACAGAATCGATAATGAAAATAATACTCTCCAGTAACTGTGCTCCTGTTCTACTATCAGCCT
GCAAAAAATACAGGACGAAAGGCAATTAGTAACACTTAAAATTTGAGATCCTATAGTGCAAACG
CCTTCACACAGTTGATTTCTTTTCTGGATATCACCACTATATTTTTAAGGAAAACAACCATAGG
TAAGTTCTTAAATCTTAACAGACTCATTCCTAGAAACTTAAAAGAATTTTGACCAACCTGACTC
TTCAGATATGGCTGTAGGGTGATGACAAACTTAGTAGGGTCTGAAGCCGGTGTACACAGCCCAG
GATCCACCAGACAGAAAGCATGCAAAACAAGTACATAAGGAAGTACTTGCACCTCTCCTTCTTC
ACGGCTCATTTCTTCTAC

Sequences
Splice junction sequence	CCGGTGTACACAGCCCAGGATCCACCAGACAGAAAGCATGCAAAACAAGTACATAAGGAAGTAC TTGCACCTCTCCTTCTTCACGGCTCATTTCTTCTACctgtgtgttatcggaaccttgggcctcc agccgcttaaaaaagaactgaagaagatgctcaactatgctaacacctttccccgccaatttac tcacagaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 432 Transcript exons: 5050936-5051044,5051212-5051381,5051569-5051721 CTGTGTGTTATCGGAACCTTGGGCCTCCAGCCGCTTAAAAAAGAACTGAAGAAGATGCTCAACT ATGCTAACACCTTTCCCCGCCAATTTACTCACAGAACAAAGACACCTAACGCAAGCATGCACAA CAGTTAGAAACGAATGCCGAACAATCATATGCTTTAGATCCTGTTCCAGATCTTCTGTGACACT TAGAGGCAGCTTGCGGATCAAGGGTAGGACAGAATCGATAATGAAAATAATACTCTCCAGTAAC TGTGCTCCTGTTCTACTATCAGCCTGACTCTTCAGATATGGCTGTAGGGTGATGACAAACTTAG TAGGGTCTGAAGCCGGTGTACACAGCCCAGGATCCACCAGACAGAAAGCATGCAAAACAAGTAC ATAAGGAAGTACTTGCACCTCTCCTTCTTCACGGCTCATTTCTTCTAC
Genomic sequence	CTGTGTGTTATCGGAACCTTGGGCCTCCAGCCGCTTAAAAAAGAACTGAAGAAGATGCTCAACT ATGCTAACACCTTTCCCCGCCAATTTACTCACAGAACAAAGACACCTGAGAATTCAAAAGGTTG CATTAGTAGTCTCCTGAAAAGGATGAGTGATTTTACTCGACTGCATCTTCTATGAAGACATGCA ATCGTCCCAAAAACAATGAACATATGTGAAAGAGAATACGATGAGTAGAGCCAGGAGTGATGGT AGTTGAAATATAGAGGGTACCTAACGCAAGCATGCACAACAGTTAGAAACGAATGCCGAACAAT CATATGCTTTAGATCCTGTTCCAGATCTTCTGTGACACTTAGAGGCAGCTTGCGGATCAAGGGT AGGACAGAATCGATAATGAAAATAATACTCTCCAGTAACTGTGCTCCTGTTCTACTATCAGCCT GCAAAAAATACAGGACGAAAGGCAATTAGTAACACTTAAAATTTGAGATCCTATAGTGCAAACG CCTTCACACAGTTGATTTCTTTTCTGGATATCACCACTATATTTTTAAGGAAAACAACCATAGG TAAGTTCTTAAATCTTAACAGACTCATTCCTAGAAACTTAAAAGAATTTTGACCAACCTGACTC TTCAGATATGGCTGTAGGGTGATGACAAACTTAGTAGGGTCTGAAGCCGGTGTACACAGCCCAG GATCCACCAGACAGAAAGCATGCAAAACAAGTACATAAGGAAGTACTTGCACCTCTCCTTCTTC ACGGCTCATTTCTTCTAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.245315191

Conservation Information
Conserved circRNAs	NA
PMCS	0.245315191

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							5051713-5050938(-)
						
						Potential amino acid sequence
						
							MSREEGEVQVLPYVLVLHAFCLVDPGLCTPASDPTKFVITLQPYLKSQADSRTGAQLLESIIFI
IDSVLPLIRKLPLSVTEDLEQDLKHMIVRHSFLTVVHACVRCLCSVSKLAGKGVSIVEHLLQFF
FKRLEAQGSDNTQVEEMSREEGEVQVLPYVLVLHAFCLVDPGLCTPASDPTKFVITLQPYLKSQ
ADSRTGAQLLESIIFIIDSVLPLIRKLPLSVTEDLEQDLKHMIVRHSFLTVVHACVRCLCSVSK
LAGKGVSIVEHLLQFFFKRLEAQGSDNTQVEEMSREEGEVQVLPYVLVLHAFCLVDPGLCTPAS
DPTKFVITLQPYLKSQADSRTGAQLLESIIFIIDSVLPLIRKLPLSVTEDLEQDLKHMIVRHSF
LTVVHACVRCLCSVSKLAGKGVSIVEHLLQFFFKRLEAQGSDNTQVEEMSREEGEVQVLPYVLV
LHAFCLVDPGLCTPASDPTKFVITLQPYLKSQADSRTGAQLLESIIFIIDSVLPLIRKLPLSVT
EDLEQDLKHMIVRHSFLTVVHACVRCLCSVSKLAGKGVSIVEHLLQFFFKRLEAQGSDNTQ(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	5051713-5050938(-)
Potential amino acid sequence	MSREEGEVQVLPYVLVLHAFCLVDPGLCTPASDPTKFVITLQPYLKSQADSRTGAQLLESIIFI IDSVLPLIRKLPLSVTEDLEQDLKHMIVRHSFLTVVHACVRCLCSVSKLAGKGVSIVEHLLQFF FKRLEAQGSDNTQVEEMSREEGEVQVLPYVLVLHAFCLVDPGLCTPASDPTKFVITLQPYLKSQ ADSRTGAQLLESIIFIIDSVLPLIRKLPLSVTEDLEQDLKHMIVRHSFLTVVHACVRCLCSVSK LAGKGVSIVEHLLQFFFKRLEAQGSDNTQVEEMSREEGEVQVLPYVLVLHAFCLVDPGLCTPAS DPTKFVITLQPYLKSQADSRTGAQLLESIIFIIDSVLPLIRKLPLSVTEDLEQDLKHMIVRHSF LTVVHACVRCLCSVSKLAGKGVSIVEHLLQFFFKRLEAQGSDNTQVEEMSREEGEVQVLPYVLV LHAFCLVDPGLCTPASDPTKFVITLQPYLKSQADSRTGAQLLESIIFIIDSVLPLIRKLPLSVT EDLEQDLKHMIVRHSFLTVVHACVRCLCSVSKLAGKGVSIVEHLLQFFFKRLEAQGSDNTQ(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015

circRNA

Detail information of AT5G15540_circ_g.2

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