Plant Circular RNA Database

Detail information of Csa1G571280_circ_g.4

			
						 General Information
					
						CircRNA Name
						Csa1G571280_circ_g.4
					
						ID in PlantcircBase
						csa_circ_000488
					
						Alias
						Chr1:21205717_21207616
					
						Organism
						
							Cucumis sativus						
					
						Position
						
							chrChr1: 21205717-21207616  JBrowse»						
					
						Reference genome
						
							Cucumis sativus v1.5						
					
						Type  
						
							e-circRNA							
									
						Identification method
						Find_circ
					
						Parent gene
						
							Csa1G571280						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							Csa1G571280_circ_g.1 Csa1G571280_circ_g.2 Csa1G571280_circ_g.3 						
					
						Support reads
						NA
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Csa1G571280.1:5
Csa1G571280.2:5

General Information
CircRNA Name	Csa1G571280_circ_g.4
ID in PlantcircBase	csa_circ_000488
Alias	Chr1:21205717_21207616
Organism	Cucumis sativus
Position	chrChr1: 21205717-21207616 JBrowse»
Reference genome	Cucumis sativus v1.5
Type	e-circRNA
Identification method	Find_circ
Parent gene	Csa1G571280
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	Csa1G571280_circ_g.1 Csa1G571280_circ_g.2 Csa1G571280_circ_g.3
Support reads	NA
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Csa1G571280.1:5 Csa1G571280.2:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TGGAGCAGTCATCTGCAGTCAAATGCCCATCCATATCTTATCATTTAGTAGGCACCAAGAAAAT
TCAACAGGAACTTGCAAGACCAAATGTTCTTGAAAGggctctacttggtgaatatgggaaagtc
ctcggattggactatagaaatgttcctgaaaactatgctgctaaccactttgctgacgtactgg
ccaaagct

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 477
Transcript exons: 21205717-21205839,21205927-21206007,21206179-21206259,21
206355-21206424,21207495-21207616
GGCTCTACTTGGTGAATATGGGAAAGTCCTCGGATTGGACTATAGAAATGTTCCTGAAAACTAT
GCTGCTAACCACTTTGCTGACGTACTGGCCAAAGCTTGGATCGAATACAATGACCCAAGTGCTG
TTATTTTGTTTGTGGTTCAAGCTGAGGAAAGAAACATGTATGATCAACACTGGCTTTCTGTTAA
CTTGAGAAAAAGACATAACGTCAAAACTCTCCGGAAAACACTGGCAGAAATCGATAGAGAGGGA
GTACTAAGATCCGATGGAAAACTCATTGTGGATGGCCAAGCAATTGCTGTTGTATACTTCAGGG
CTGGATATACGCCTAGGGACTATCCGTCTGAATCGGAATGGAGAGCAAGGTTACTTATGGAGCA
GTCATCTGCAGTCAAATGCCCATCCATATCTTATCATTTAGTAGGCACCAAGAAAATTCAACAG
GAACTTGCAAGACCAAATGTTCTTGAAAG
						
					

						Genomic sequence
						
							GGCTCTACTTGGTGAATATGGGAAAGTCCTCGGATTGGACTATAGAAATGTTCCTGAAAACTAT
GCTGCTAACCACTTTGCTGACGTACTGGCCAAAGCTTGGATCGAATACAATGACCCAAGGTTAG
ATTTGATAATTTGATCTTCTTATTCATCTCCATTTCTAGAAATTTATTGCATCACATGTAACAT
GGGTTATATTTTCTGCAGTGCTGTTATTTTGTTTGTGGTTCAAGCTGAGGAAAGAAACATGTAT
GATCAACACTGGCTTTCTGTTAACTTGAGAAAAAGATATCCACTTTTGCCGGATCTACTTTATA
AAGCATTGATCAATTTTCTTAATTTTCAATTTACTTCTGACTAAAACTATACAGATTGAAAATA
AATTGTTGCTTTTTGACAATTTGATCAATTTGGTACATCTTGAGCTTGCCAAGTAAATATTCCT
TAGCTTTATTATACACATAACGTCAAAACTCTCCGGAAAACACTGGCAGAAATCGATAGAGAGG
GAGTACTAAGATCCGATGGAAAACTCATTGTGTAAGTAATGTTGAATGTCAAATTTGGTTTTTC
CTTCATAAAATTGGTTACAGATTTGTGTTTCTTATAACTCTTGAGAACTTCTCCTCCTGCAGGG
ATGGCCAAGCAATTGCTGTTGTATACTTCAGGGCTGGATATACGCCTAGGGACTATCCGTCTGA
ATCGGTGAGCAACTGCTTCATTCTGCTATTGGTCACAGTCGCATGAGACCTTTTTTTTTGTTAA
GAATTAGGATTAAGGTGATGTATTCTTTTGGGATGGGCTTGCTGTTGCTACCATGCATTAGTGT
TTCCATTCATTCTAGGAAGAAGCTCACTTTTCTTGATGGATTTCAATAGTTTCATGAGATTTTA
AGACAGGGAAAAGTAAATTCAATCCCGGAGGATTTTAGAGTTCCAGAAGTTAAATTTCTTAGCA
ACTTTGTAAGACCTTTCTTCATGCTGGCTTTGTGGATGTACCGGAAACCCATTGGCTCTTCTTC
TAACTTAAAGTTCTTGTATGAAAATGTTTGGGAGAAATAAATTATGTAATCTGAAGTGTTGAAC
CTTCACAATAATTGAAACAATGAGAGTGCAAACTCCAGAATCCTCCCCTTTGTCATCTTATGAA
TTTATCTGCTCAAACTTGAATAACTTATAGATCATTCTAGATGAAGTCTTGGAATTACATTCAT
CAGAGGATAAATGTAGATTTTGATTTTCCTTGATCGTGCTCATTAGATTTTGCAGTTGTCTTTC
GTCAGCATATCCTATATCTTCCCCACTGTTGTCAAACCAATGCTGATAGTGACTGTGTGCATGT
TCGATTTTTGCTAGGTACTAAGCGATTTGGTGTGCTTAAGTTACAAAATGGATTGTATCGTAGA
TGTAATATTACCTGTCCTGACTCATAATAATACTCCCTGAGTTGCATGGCATTAAAAAATTCAG
AAGTCTCTCTTACTCCAGCTCATAGAAGCTTGAGTGTAGGGGCCCTCTAAATAGGCATTAAAAT
TTACCAACACCAACCCATTGACCCAGGTAATGTCGAAGCAATTCTAAACTATTCATATATACAG
ACAATTATAACATCTGATGATAATCCTTCTAATTTGATGACCATTATCAATTTCAATACCAAGG
AATAAAAGAGTCTCTCTTAAAGTTCTTTAACTTCCTTGTCAATTTATTAAAGTTAATTTTATCT
TGAATAAAGCTTCGTAGTTATTTTCTCATTTGAATGGTAACAAATTTCAGGAATGGAGAGCAAG
GTTACTTATGGAGCAGTCATCTGCAGTCAAATGCCCATCCATATCTTATCATTTAGTAGGCACC
AAGAAAATTCAACAGGAACTTGCAAGACCAAATGTTCTTGAAAG
						
					

			
		

Sequences
Splice junction sequence	TGGAGCAGTCATCTGCAGTCAAATGCCCATCCATATCTTATCATTTAGTAGGCACCAAGAAAAT TCAACAGGAACTTGCAAGACCAAATGTTCTTGAAAGggctctacttggtgaatatgggaaagtc ctcggattggactatagaaatgttcctgaaaactatgctgctaaccactttgctgacgtactgg ccaaagct
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 477 Transcript exons: 21205717-21205839,21205927-21206007,21206179-21206259,21 206355-21206424,21207495-21207616 GGCTCTACTTGGTGAATATGGGAAAGTCCTCGGATTGGACTATAGAAATGTTCCTGAAAACTAT GCTGCTAACCACTTTGCTGACGTACTGGCCAAAGCTTGGATCGAATACAATGACCCAAGTGCTG TTATTTTGTTTGTGGTTCAAGCTGAGGAAAGAAACATGTATGATCAACACTGGCTTTCTGTTAA CTTGAGAAAAAGACATAACGTCAAAACTCTCCGGAAAACACTGGCAGAAATCGATAGAGAGGGA GTACTAAGATCCGATGGAAAACTCATTGTGGATGGCCAAGCAATTGCTGTTGTATACTTCAGGG CTGGATATACGCCTAGGGACTATCCGTCTGAATCGGAATGGAGAGCAAGGTTACTTATGGAGCA GTCATCTGCAGTCAAATGCCCATCCATATCTTATCATTTAGTAGGCACCAAGAAAATTCAACAG GAACTTGCAAGACCAAATGTTCTTGAAAG
Genomic sequence	GGCTCTACTTGGTGAATATGGGAAAGTCCTCGGATTGGACTATAGAAATGTTCCTGAAAACTAT GCTGCTAACCACTTTGCTGACGTACTGGCCAAAGCTTGGATCGAATACAATGACCCAAGGTTAG ATTTGATAATTTGATCTTCTTATTCATCTCCATTTCTAGAAATTTATTGCATCACATGTAACAT GGGTTATATTTTCTGCAGTGCTGTTATTTTGTTTGTGGTTCAAGCTGAGGAAAGAAACATGTAT GATCAACACTGGCTTTCTGTTAACTTGAGAAAAAGATATCCACTTTTGCCGGATCTACTTTATA AAGCATTGATCAATTTTCTTAATTTTCAATTTACTTCTGACTAAAACTATACAGATTGAAAATA AATTGTTGCTTTTTGACAATTTGATCAATTTGGTACATCTTGAGCTTGCCAAGTAAATATTCCT TAGCTTTATTATACACATAACGTCAAAACTCTCCGGAAAACACTGGCAGAAATCGATAGAGAGG GAGTACTAAGATCCGATGGAAAACTCATTGTGTAAGTAATGTTGAATGTCAAATTTGGTTTTTC CTTCATAAAATTGGTTACAGATTTGTGTTTCTTATAACTCTTGAGAACTTCTCCTCCTGCAGGG ATGGCCAAGCAATTGCTGTTGTATACTTCAGGGCTGGATATACGCCTAGGGACTATCCGTCTGA ATCGGTGAGCAACTGCTTCATTCTGCTATTGGTCACAGTCGCATGAGACCTTTTTTTTTGTTAA GAATTAGGATTAAGGTGATGTATTCTTTTGGGATGGGCTTGCTGTTGCTACCATGCATTAGTGT TTCCATTCATTCTAGGAAGAAGCTCACTTTTCTTGATGGATTTCAATAGTTTCATGAGATTTTA AGACAGGGAAAAGTAAATTCAATCCCGGAGGATTTTAGAGTTCCAGAAGTTAAATTTCTTAGCA ACTTTGTAAGACCTTTCTTCATGCTGGCTTTGTGGATGTACCGGAAACCCATTGGCTCTTCTTC TAACTTAAAGTTCTTGTATGAAAATGTTTGGGAGAAATAAATTATGTAATCTGAAGTGTTGAAC CTTCACAATAATTGAAACAATGAGAGTGCAAACTCCAGAATCCTCCCCTTTGTCATCTTATGAA TTTATCTGCTCAAACTTGAATAACTTATAGATCATTCTAGATGAAGTCTTGGAATTACATTCAT CAGAGGATAAATGTAGATTTTGATTTTCCTTGATCGTGCTCATTAGATTTTGCAGTTGTCTTTC GTCAGCATATCCTATATCTTCCCCACTGTTGTCAAACCAATGCTGATAGTGACTGTGTGCATGT TCGATTTTTGCTAGGTACTAAGCGATTTGGTGTGCTTAAGTTACAAAATGGATTGTATCGTAGA TGTAATATTACCTGTCCTGACTCATAATAATACTCCCTGAGTTGCATGGCATTAAAAAATTCAG AAGTCTCTCTTACTCCAGCTCATAGAAGCTTGAGTGTAGGGGCCCTCTAAATAGGCATTAAAAT TTACCAACACCAACCCATTGACCCAGGTAATGTCGAAGCAATTCTAAACTATTCATATATACAG ACAATTATAACATCTGATGATAATCCTTCTAATTTGATGACCATTATCAATTTCAATACCAAGG AATAAAAGAGTCTCTCTTAAAGTTCTTTAACTTCCTTGTCAATTTATTAAAGTTAATTTTATCT TGAATAAAGCTTCGTAGTTATTTTCTCATTTGAATGGTAACAAATTTCAGGAATGGAGAGCAAG GTTACTTATGGAGCAGTCATCTGCAGTCAAATGCCCATCCATATCTTATCATTTAGTAGGCACC AAGAAAATTCAACAGGAACTTGCAAGACCAAATGTTCTTGAAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.473997184

Conservation Information
Conserved circRNAs	NA
PMCS	0.473997184

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							21205968-21207613(+)
21207605-21205997(+)
						
						Potential amino acid sequence
						
							MYDQHWLSVNLRKRHNVKTLRKTLAEIDREGVLRSDGKLIVDGQAIAVVYFRAGYTPRDYPSES
EWRARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERALLGEYGKVLGLDYRNVPENYAA
NHFADVLAKAWIEYNDPSAVILFVVQAEERNMYDQHWLSVNLRKRHNVKTLRKTLAEIDREGVL
RSDGKLIVDGQAIAVVYFRAGYTPRDYPSESEWRARLLMEQSSAVKCPSISYHLVGTKKIQQEL
ARPNVLERALLGEYGKVLGLDYRNVPENYAANHFADVLAKAWIEYNDPSAVILFVVQAEERNMY
DQHWLSVNLRKRHNVKTLRKTLAEIDREGVLRSDGKLIVDGQAIAVVYFRAGYTPRDYPSESEW
RARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERALLGEYGKVLGLDYRNVPENYAANH
FADVLAKAWIEYNDPSAVILFVVQAEERNMYDQHWLSVNLRKRHNVKTLRKTLAEIDREGVLRS
DGKLIVDGQAIAVVYFRAGYTPRDYPSESEWRARLLMEQSSAVKCPSISYHLVGTKKIQQELAR
PNVLE
MFLKGLYLVNMGKSSDWTIEMFLKTMLLTTLLTYWPKLGSNTMTQVLLFCLWFKLRKETCMINT
GFLLT*
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	21205968-21207613(+) 21207605-21205997(+)
Potential amino acid sequence	MYDQHWLSVNLRKRHNVKTLRKTLAEIDREGVLRSDGKLIVDGQAIAVVYFRAGYTPRDYPSES EWRARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERALLGEYGKVLGLDYRNVPENYAA NHFADVLAKAWIEYNDPSAVILFVVQAEERNMYDQHWLSVNLRKRHNVKTLRKTLAEIDREGVL RSDGKLIVDGQAIAVVYFRAGYTPRDYPSESEWRARLLMEQSSAVKCPSISYHLVGTKKIQQEL ARPNVLERALLGEYGKVLGLDYRNVPENYAANHFADVLAKAWIEYNDPSAVILFVVQAEERNMY DQHWLSVNLRKRHNVKTLRKTLAEIDREGVLRSDGKLIVDGQAIAVVYFRAGYTPRDYPSESEW RARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERALLGEYGKVLGLDYRNVPENYAANH FADVLAKAWIEYNDPSAVILFVVQAEERNMYDQHWLSVNLRKRHNVKTLRKTLAEIDREGVLRS DGKLIVDGQAIAVVYFRAGYTPRDYPSESEWRARLLMEQSSAVKCPSISYHLVGTKKIQQELAR PNVLE MFLKGLYLVNMGKSSDWTIEMFLKTMLLTTLLTYWPKLGSNTMTQVLLFCLWFKLRKETCMINT GFLLT*
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						He et al., 2020

circRNA

Detail information of Csa1G571280_circ_g.4

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