Plant Circular RNA Database

Detail information of AT5G27550_circ_g.2

			
						 General Information
					
						CircRNA Name
						AT5G27550_circ_g.2
					
						ID in PlantcircBase
						ath_circ_040781
					
						Alias
						5:9728462-9729534
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 9728462-9729534  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ; CIRI2
					
						Parent gene
						
							AT5G27550						
					
						Parent gene annotation
						
							Kinesin-like protein KIN-14S
							
						Parent gene strand
						-
					
						Alternative splicing
						
							AT5G27550_circ_g.1 AT5G27550_circ_g.3 AT5G27550_circ_g.4 AT5G27550_circ_g.5 						
					
						Support reads
						1/1
					
						Tissues
						seedling;root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G27550.2:5
AT5G27550.1:5

General Information
CircRNA Name	AT5G27550_circ_g.2
ID in PlantcircBase	ath_circ_040781
Alias	5:9728462-9729534
Organism	Arabidpsis thaliana
Position	chr5: 9728462-9729534 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	find_circ; CIRI2
Parent gene	AT5G27550
Parent gene annotation	Kinesin-like protein KIN-14S
Parent gene strand	-
Alternative splicing	AT5G27550_circ_g.1 AT5G27550_circ_g.3 AT5G27550_circ_g.4 AT5G27550_circ_g.5
Support reads	1/1
Tissues	seedling;root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G27550.2:5 AT5G27550.1:5

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							TTTCTTGAGCAGATCCCACACTCCATCTGTATTGTAAACTTGAGCTTCGACTAATCCTGGGACT
TCTTGCGTCCCTTCTGCTGATTGCTTAACCTCCAACcttatcttgaagtcctcggcatatgtgt
tctctagcagtgaggcgtagctgtagtgactgcacattgtcctgcagcttctttgtttcctttt
cctcgtgt

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 694
Transcript exons: 9728462-9728575,9728661-9728824,9728932-9728969,9729070-
9729285,9729373-9729534
CTTATCTTGAAGTCCTCGGCATATGTGTTCTCTAGCAGTGAGGCGTAGCTGTAGTGACTGCACA
TTGTCCTGCAGCTTCTTTGTTTCCTTTTCCTCGTGTTTCAGCTTCTCGGCCATTTGTTTTGACT
TAAGGAGTTCAGAGACATCAGCCTGTTTTCGGGCAGGTCCACTTTCGATTCCTCGGACTCTACT
TGCAAAGTTCAAGGAGCAAAGGGTCTCTCCTAGATCGGCAGAACTCGGACTAATCTGGACAAAC
ATTAATGTCTTGCAATCTCCACCCAAAGAGTTTTGCAGCATATGGGTCAGCTTTGAGTTTCTGT
AAGGAATGTGGCTTGTTTTGGATGCAAGGGCAGAGATAACATCACCGAGTGCTGAAAGCGACTT
ATTGATAAATTGAGATTCTTTCAGCCTTTCTCCTTCAACTTCTACCTTTCCTACTCGCTCACTG
CCAGCCAAGTCCACTAACCAAAGATGACTTCTCGTTCTCTGACCATTTATCAAATTCTCTCCCT
TCACTGTCACTCGCAGCAAGCAATGAGAGCGGCTGCTTTGCTCATTTGCAGCAGTTGAACCCAC
AGATCGAACAGCATATCCTTTCTTGAGCAGATCCCACACTCCATCTGTATTGTAAACTTGAGCT
TCGACTAATCCTGGGACTTCTTGCGTCCCTTCTGCTGATTGCTTAACCTCCAAC
						
					

						Genomic sequence
						
							CTTATCTTGAAGTCCTCGGCATATGTGTTCTCTAGCAGTGAGGCGTAGCTGTAGTGACTGCACA
TTGTCCTGCAGCTTCTTTGTTTCCTTTTCCTCGTGTTTCAGCTTCTCGGCCTGTTACAAAGGAT
ACAACAAAAAATGAAATAACCTTAAGATATGTTAAAGCTTAGATGAACTGGAGTTCGAGGAATC
AAGTTACCATTTGTTTTGACTTAAGGAGTTCAGAGACATCAGCCTGTTTTCGGGCAGGTCCACT
TTCGATTCCTCGGACTCTACTTGCAAAGTTCAAGGAGCAAAGGGTCTCTCCTAGATCGGCAGAA
CTCGGACTAATCTGGACAAACATTAATGTCTTGCAATCTCCACCTGTATAAGTCACAATGAAAT
AGAAACACCATGGAGATGAAAAATAAGATGTCTTATGAATCATGTGGCCATAACAACGGAAGAA
AATAGGACAAATGTTACTTTACCCAAAGAGTTTTGCAGCATATGGGTCAGCTTTGAGTTTCTGG
AGTTTGACAAAAAGGAAGGAAAAAAGTGTCATCAACATCAATCAGAAAATCTCATATGCAAATG
TCTTAACAAAGGGGAAGAAAGAAGGGAAAAACCTGTAAGGAATGTGGCTTGTTTTGGATGCAAG
GGCAGAGATAACATCACCGAGTGCTGAAAGCGACTTATTGATAAATTGAGATTCTTTCAGCCTT
TCTCCTTCAACTTCTACCTTTCCTACTCGCTCACTGCCAGCCAAGTCCACTAACCAAAGATGAC
TTCTCGTTCTCTGACCATTTATCAAATTCTCTCCCTTCACTGTCACTCGCAGCAAGCTGAACAA
AAGGAAATCTCTTGTTGGTATAAGTATGGAAAAGGTTTAGTTTCACATGAATTTAGTTTCTAAA
CTCTAGTTTGCTTACCAATGAGAGCGGCTGCTTTGCTCATTTGCAGCAGTTGAACCCACAGATC
GAACAGCATATCCTTTCTTGAGCAGATCCCACACTCCATCTGTATTGTAAACTTGAGCTTCGAC
TAATCCTGGGACTTCTTGCGTCCCTTCTGCTGATTGCTTAACCTCCAAC
						
					

			
		

Sequences
Splice junction sequence	TTTCTTGAGCAGATCCCACACTCCATCTGTATTGTAAACTTGAGCTTCGACTAATCCTGGGACT TCTTGCGTCCCTTCTGCTGATTGCTTAACCTCCAACcttatcttgaagtcctcggcatatgtgt tctctagcagtgaggcgtagctgtagtgactgcacattgtcctgcagcttctttgtttcctttt cctcgtgt
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 694 Transcript exons: 9728462-9728575,9728661-9728824,9728932-9728969,9729070- 9729285,9729373-9729534 CTTATCTTGAAGTCCTCGGCATATGTGTTCTCTAGCAGTGAGGCGTAGCTGTAGTGACTGCACA TTGTCCTGCAGCTTCTTTGTTTCCTTTTCCTCGTGTTTCAGCTTCTCGGCCATTTGTTTTGACT TAAGGAGTTCAGAGACATCAGCCTGTTTTCGGGCAGGTCCACTTTCGATTCCTCGGACTCTACT TGCAAAGTTCAAGGAGCAAAGGGTCTCTCCTAGATCGGCAGAACTCGGACTAATCTGGACAAAC ATTAATGTCTTGCAATCTCCACCCAAAGAGTTTTGCAGCATATGGGTCAGCTTTGAGTTTCTGT AAGGAATGTGGCTTGTTTTGGATGCAAGGGCAGAGATAACATCACCGAGTGCTGAAAGCGACTT ATTGATAAATTGAGATTCTTTCAGCCTTTCTCCTTCAACTTCTACCTTTCCTACTCGCTCACTG CCAGCCAAGTCCACTAACCAAAGATGACTTCTCGTTCTCTGACCATTTATCAAATTCTCTCCCT TCACTGTCACTCGCAGCAAGCAATGAGAGCGGCTGCTTTGCTCATTTGCAGCAGTTGAACCCAC AGATCGAACAGCATATCCTTTCTTGAGCAGATCCCACACTCCATCTGTATTGTAAACTTGAGCT TCGACTAATCCTGGGACTTCTTGCGTCCCTTCTGCTGATTGCTTAACCTCCAAC
Genomic sequence	CTTATCTTGAAGTCCTCGGCATATGTGTTCTCTAGCAGTGAGGCGTAGCTGTAGTGACTGCACA TTGTCCTGCAGCTTCTTTGTTTCCTTTTCCTCGTGTTTCAGCTTCTCGGCCTGTTACAAAGGAT ACAACAAAAAATGAAATAACCTTAAGATATGTTAAAGCTTAGATGAACTGGAGTTCGAGGAATC AAGTTACCATTTGTTTTGACTTAAGGAGTTCAGAGACATCAGCCTGTTTTCGGGCAGGTCCACT TTCGATTCCTCGGACTCTACTTGCAAAGTTCAAGGAGCAAAGGGTCTCTCCTAGATCGGCAGAA CTCGGACTAATCTGGACAAACATTAATGTCTTGCAATCTCCACCTGTATAAGTCACAATGAAAT AGAAACACCATGGAGATGAAAAATAAGATGTCTTATGAATCATGTGGCCATAACAACGGAAGAA AATAGGACAAATGTTACTTTACCCAAAGAGTTTTGCAGCATATGGGTCAGCTTTGAGTTTCTGG AGTTTGACAAAAAGGAAGGAAAAAAGTGTCATCAACATCAATCAGAAAATCTCATATGCAAATG TCTTAACAAAGGGGAAGAAAGAAGGGAAAAACCTGTAAGGAATGTGGCTTGTTTTGGATGCAAG GGCAGAGATAACATCACCGAGTGCTGAAAGCGACTTATTGATAAATTGAGATTCTTTCAGCCTT TCTCCTTCAACTTCTACCTTTCCTACTCGCTCACTGCCAGCCAAGTCCACTAACCAAAGATGAC TTCTCGTTCTCTGACCATTTATCAAATTCTCTCCCTTCACTGTCACTCGCAGCAAGCTGAACAA AAGGAAATCTCTTGTTGGTATAAGTATGGAAAAGGTTTAGTTTCACATGAATTTAGTTTCTAAA CTCTAGTTTGCTTACCAATGAGAGCGGCTGCTTTGCTCATTTGCAGCAGTTGAACCCACAGATC GAACAGCATATCCTTTCTTGAGCAGATCCCACACTCCATCTGTATTGTAAACTTGAGCTTCGAC TAATCCTGGGACTTCTTGCGTCCCTTCTGCTGATTGCTTAACCTCCAAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.326616118

Conservation Information
Conserved circRNAs	NA
PMCS	0.326616118

			
						 Functional Information
					
						Coding potential
						NA
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	NA
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Philips et al., 2020

circRNA

Detail information of AT5G27550_circ_g.2

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