Plant Circular RNA Database

Detail information of Os02g0194900_circ_g.2

			
						 General Information
					
						CircRNA Name
						Os02g0194900_circ_g.2
					
						ID in PlantcircBase
						osa_circ_013626
					
						Alias
						Os_ciR1168, 
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 5321779-5322039  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						circseq_cup, find_circ, CIRI-long
					
						Parent gene
						
							Os02g0194900						
					
						Parent gene annotation
						
							Basic-leucine zipper (bZIP) transcription factor domain containi
ng protein. (Os02t0194900-00)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os02g0194900_circ_g.3 						
					
						Support reads
						15/6
					
						Tissues
						root/root, root, leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os02t0194900-00:1

General Information
CircRNA Name	Os02g0194900_circ_g.2
ID in PlantcircBase	osa_circ_013626
Alias	Os_ciR1168,
Organism	Oryza sativa
Position	chr2: 5321779-5322039 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	circseq_cup, find_circ, CIRI-long
Parent gene	Os02g0194900
Parent gene annotation	Basic-leucine zipper (bZIP) transcription factor domain containi ng protein. (Os02t0194900-00)
Parent gene strand	+
Alternative splicing	Os02g0194900_circ_g.3
Support reads	15/6
Tissues	root/root, root, leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os02t0194900-00:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CCCTCCCGGCACCGGTGCCACCGCATACTCCGACGTCGCCATGGCTCACCTCTCCCTTGCCATC
TCCAATCTCTCCTCCCTCGAAGCCTTCGTCAGGCAGatgatggcgcggcacgcggagccattga
cggagcaacaggcggcgggtgtgtacggtgtgcagcagtcggcgagggagagggaggaggcact
tgaccgcg

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 261
Transcript exons: 5321779-5322039
ATGATGGCGCGGCACGCGGAGCCATTGACGGAGCAACAGGCGGCGGGTGTGTACGGTGTGCAGC
AGTCGGCGAGGGAGAGGGAGGAGGCACTTGACCGCGACCTCCACGCCACCCACCACGCCCTCTC
CGATGCCGTCTCCTCGGACTCACTCCTGCTCTTCCCTCCCGGCACCGGTGCCACCGCATACTCC
GACGTCGCCATGGCTCACCTCTCCCTTGCCATCTCCAATCTCTCCTCCCTCGAAGCCTTCGTCA
GGCAG
						
						Genomic sequence
						
							ATGATGGCGCGGCACGCGGAGCCATTGACGGAGCAACAGGCGGCGGGTGTGTACGGTGTGCAGC
AGTCGGCGAGGGAGAGGGAGGAGGCACTTGACCGCGACCTCCACGCCACCCACCACGCCCTCTC
CGATGCCGTCTCCTCGGACTCACTCCTGCTCTTCCCTCCCGGCACCGGTGCCACCGCATACTCC
GACGTCGCCATGGCTCACCTCTCCCTTGCCATCTCCAATCTCTCCTCCCTCGAAGCCTTCGTCA
GGCAG

Sequences
Splice junction sequence	CCCTCCCGGCACCGGTGCCACCGCATACTCCGACGTCGCCATGGCTCACCTCTCCCTTGCCATC TCCAATCTCTCCTCCCTCGAAGCCTTCGTCAGGCAGatgatggcgcggcacgcggagccattga cggagcaacaggcggcgggtgtgtacggtgtgcagcagtcggcgagggagagggaggaggcact tgaccgcg
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 261 Transcript exons: 5321779-5322039 ATGATGGCGCGGCACGCGGAGCCATTGACGGAGCAACAGGCGGCGGGTGTGTACGGTGTGCAGC AGTCGGCGAGGGAGAGGGAGGAGGCACTTGACCGCGACCTCCACGCCACCCACCACGCCCTCTC CGATGCCGTCTCCTCGGACTCACTCCTGCTCTTCCCTCCCGGCACCGGTGCCACCGCATACTCC GACGTCGCCATGGCTCACCTCTCCCTTGCCATCTCCAATCTCTCCTCCCTCGAAGCCTTCGTCA GGCAG
Genomic sequence	ATGATGGCGCGGCACGCGGAGCCATTGACGGAGCAACAGGCGGCGGGTGTGTACGGTGTGCAGC AGTCGGCGAGGGAGAGGGAGGAGGCACTTGACCGCGACCTCCACGCCACCCACCACGCCCTCTC CGATGCCGTCTCCTCGGACTCACTCCTGCTCTTCCCTCCCGGCACCGGTGCCACCGCATACTCC GACGTCGCCATGGCTCACCTCTCCCTTGCCATCTCCAATCTCTCCTCCCTCGAAGCCTTCGTCA GGCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.441644093

Conservation Information
Conserved circRNAs	NA
PMCS	0.441644093

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							5321780-5322036(+)
5321910-5321779(+)
5321983-5321987(-)
5321967-5322034(-)
						
						Potential amino acid sequence
						
							MMARHAEPLTEQQAAGVYGVQQSAREREEALDRDLHATHHALSDAVSSDSLLLFPPGTGATAYS
DVAMAHLSLAISNLSSLEAFVRQMMARHAEPLTEQQAAGVYGVQQSAREREEALDRDLHATHHA
LSDAVSSDSLLLFPPGTGATAYSDVAMAHLSLAISNLSSLEAFVRQMMARHAEPLTEQQAAGVY
GVQQSAREREEALDRDLHATHHALSDAVSSDSLLLFPPGTGATAYSDVAMAHLSLAISNLSSLE
AFVRQMMARHAEPLTEQQAAGVYGVQQSAREREEALDRDLHATHHALSDAVSSDSLLLFPPGTG
ATAYSDVAMAHLSLAISNLSSLEAFVRQ(+)
MPSPRTHSCSSLPAPVPPHTPTSPWLTSPLPSPISPPSKPSSGR*(+)
MATSEYAVAPVPGGKSRSESEETASERAWWVAWRSRSSASSLSLADCCTPYTPAACCSVNGSAC
RAIICLTKASREERLEMARER*(-)
MRWHRCREGRAGVSPRRRHRRGRGGWRGGRGQVPPPSPSPTAAHRTHPPPVAPSMAPRAAPSSA
*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	5321780-5322036(+) 5321910-5321779(+) 5321983-5321987(-) 5321967-5322034(-)
Potential amino acid sequence	MMARHAEPLTEQQAAGVYGVQQSAREREEALDRDLHATHHALSDAVSSDSLLLFPPGTGATAYS DVAMAHLSLAISNLSSLEAFVRQMMARHAEPLTEQQAAGVYGVQQSAREREEALDRDLHATHHA LSDAVSSDSLLLFPPGTGATAYSDVAMAHLSLAISNLSSLEAFVRQMMARHAEPLTEQQAAGVY GVQQSAREREEALDRDLHATHHALSDAVSSDSLLLFPPGTGATAYSDVAMAHLSLAISNLSSLE AFVRQMMARHAEPLTEQQAAGVYGVQQSAREREEALDRDLHATHHALSDAVSSDSLLLFPPGTG ATAYSDVAMAHLSLAISNLSSLEAFVRQ(+) MPSPRTHSCSSLPAPVPPHTPTSPWLTSPLPSPISPPSKPSSGR(+) MATSEYAVAPVPGGKSRSESEETASERAWWVAWRSRSSASSLSLADCCTPYTPAACCSVNGSAC RAIICLTKASREERLEMARER(-) MRWHRCREGRAGVSPRRRHRRGRGGWRGGRGQVPPPSPSPTAAHRTHPPPVAPSMAPRAAPSSA *(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Ye et al., 2016; this study

circRNA

Detail information of Os02g0194900_circ_g.2

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