Plant Circular RNA Database

Detail information of Os10g0518300_circ_g.8

			
						 General Information
					
						CircRNA Name
						Os10g0518300_circ_g.8
					
						ID in PlantcircBase
						osa_circ_007693
					
						Alias
						Os_ciR6854
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr10: 20036093-20036513  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os10g0518300						
					
						Parent gene annotation
						
							Tetratricopeptide-like helical domain containing protein. (Os10t
0518300-01)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os10g0518300_circ_g.7 						
					
						Support reads
						2/3
					
						Tissues
						root/root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Os10t0518300-01:2

General Information
CircRNA Name	Os10g0518300_circ_g.8
ID in PlantcircBase	osa_circ_007693
Alias	Os_ciR6854
Organism	Oryza sativa
Position	chr10: 20036093-20036513 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os10g0518300
Parent gene annotation	Tetratricopeptide-like helical domain containing protein. (Os10t 0518300-01)
Parent gene strand	+
Alternative splicing	Os10g0518300_circ_g.7
Support reads	2/3
Tissues	root/root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Os10t0518300-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							AGTTGTTGCAGACATTGTTTCTGTGTCAGCATGCGCTGATCTAACCCTGCCACCCGGTGCTGGC
CTTCGCATCGATACTATTCATGGCCCTAGATTCTTGctgcctttgccatatctcaaagtgggat
atttgattgctcctccagaaaatagtgccattgcaccacacaaagaatgggagaggtcacagtt
tgttttga

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 291
Transcript exons: 20036093-20036212,20036343-20036513
CTGCCTTTGCCATATCTCAAAGTGGGATATTTGATTGCTCCTCCAGAAAATAGTGCCATTGCAC
CACACAAAGAATGGGAGAGGTCACAGTTTGTTTTGAACCATGAAGAACTCCAGCAGGTCAATGC
CTCTGACCAGCCTCCATCACAATCACCTGGGCATGTGGACAGCGGCAGAAAGCTTTTCAGGATA
GTTGTTGCAGACATTGTTTCTGTGTCAGCATGCGCTGATCTAACCCTGCCACCCGGTGCTGGCC
TTCGCATCGATACTATTCATGGCCCTAGATTCTTG
						
						Genomic sequence
						
							CTGCCTTTGCCATATCTCAAAGTGGGATATTTGATTGCTCCTCCAGAAAATAGTGCCATTGCAC
CACACAAAGAATGGGAGAGGTCACAGTTTGTTTTGAACCATGAAGAACTCCAGCAGGTAGTTCT
GCCACTGTTCTTTGTGCACTTGCTTTAGATGATAATATTGTCCATTTTGAAGTTACTGCTGTCT
GTAATTGTTCTTTGTCATTATCGTGCCTGCTCAAACTGCTATGTGTTGTAAATTGTAGGTCAAT
GCCTCTGACCAGCCTCCATCACAATCACCTGGGCATGTGGACAGCGGCAGAAAGCTTTTCAGGA
TAGTTGTTGCAGACATTGTTTCTGTGTCAGCATGCGCTGATCTAACCCTGCCACCCGGTGCTGG
CCTTCGCATCGATACTATTCATGGCCCTAGATTCTTG

Sequences
Splice junction sequence	AGTTGTTGCAGACATTGTTTCTGTGTCAGCATGCGCTGATCTAACCCTGCCACCCGGTGCTGGC CTTCGCATCGATACTATTCATGGCCCTAGATTCTTGctgcctttgccatatctcaaagtgggat atttgattgctcctccagaaaatagtgccattgcaccacacaaagaatgggagaggtcacagtt tgttttga
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 291 Transcript exons: 20036093-20036212,20036343-20036513 CTGCCTTTGCCATATCTCAAAGTGGGATATTTGATTGCTCCTCCAGAAAATAGTGCCATTGCAC CACACAAAGAATGGGAGAGGTCACAGTTTGTTTTGAACCATGAAGAACTCCAGCAGGTCAATGC CTCTGACCAGCCTCCATCACAATCACCTGGGCATGTGGACAGCGGCAGAAAGCTTTTCAGGATA GTTGTTGCAGACATTGTTTCTGTGTCAGCATGCGCTGATCTAACCCTGCCACCCGGTGCTGGCC TTCGCATCGATACTATTCATGGCCCTAGATTCTTG
Genomic sequence	CTGCCTTTGCCATATCTCAAAGTGGGATATTTGATTGCTCCTCCAGAAAATAGTGCCATTGCAC CACACAAAGAATGGGAGAGGTCACAGTTTGTTTTGAACCATGAAGAACTCCAGCAGGTAGTTCT GCCACTGTTCTTTGTGCACTTGCTTTAGATGATAATATTGTCCATTTTGAAGTTACTGCTGTCT GTAATTGTTCTTTGTCATTATCGTGCCTGCTCAAACTGCTATGTGTTGTAAATTGTAGGTCAAT GCCTCTGACCAGCCTCCATCACAATCACCTGGGCATGTGGACAGCGGCAGAAAGCTTTTCAGGA TAGTTGTTGCAGACATTGTTTCTGTGTCAGCATGCGCTGATCTAACCCTGCCACCCGGTGCTGG CCTTCGCATCGATACTATTCATGGCCCTAGATTCTTG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.376272011

Conservation Information
Conserved circRNAs	NA
PMCS	0.376272011

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							20036487-20036497(-)
						
						Potential amino acid sequence
						
							MRRPAPGGRVRSAHADTETMSATTILKSFLPLSTCPGDCDGGWSEALTCWSSSWFKTNCDLSHS
LCGAMALFSGGAIKYPTLRYGKGSKNLGP*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	20036487-20036497(-)
Potential amino acid sequence	MRRPAPGGRVRSAHADTETMSATTILKSFLPLSTCPGDCDGGWSEALTCWSSSWFKTNCDLSHS LCGAMALFSGGAIKYPTLRYGKGSKNLGP*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os10g0518300_circ_g.8

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