Plant Circular RNA Database

Detail information of AT1G65930_circ_g.6

			
						 General Information
					
						CircRNA Name
						AT1G65930_circ_g.6
					
						ID in PlantcircBase
						ath_circ_008857
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr1: 24540107-24540340  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						KNIFE, PcircRNA_finder
					
						Parent gene
						
							AT1G65930						
					
						Parent gene annotation
						
							Isocitrate dehydrogenase
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT1G65930_circ_g.2 AT1G65930_circ_g.3 AT1G65930_circ_g.4 AT1G65930_circ_g.5 AT1G65930_circ_g.7 AT1G65930_circ_g.8 AT1G65930_circ_g.9 AT1G65930_circ_g.10 AT1G65930_circ_g.11 AT1G65930_circ_g.12 AT1G65930_circ_g.13 						
					
						Support reads
						5
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT1G65930.1:2

General Information
CircRNA Name	AT1G65930_circ_g.6
ID in PlantcircBase	ath_circ_008857
Alias	NA
Organism	Arabidpsis thaliana
Position	chr1: 24540107-24540340 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	KNIFE, PcircRNA_finder
Parent gene	AT1G65930
Parent gene annotation	Isocitrate dehydrogenase
Parent gene strand	+
Alternative splicing	AT1G65930_circ_g.2 AT1G65930_circ_g.3 AT1G65930_circ_g.4 AT1G65930_circ_g.5 AT1G65930_circ_g.7 AT1G65930_circ_g.8 AT1G65930_circ_g.9 AT1G65930_circ_g.10 AT1G65930_circ_g.11 AT1G65930_circ_g.12 AT1G65930_circ_g.13
Support reads	5
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT1G65930.1:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TGGACAAAGCCCATCTGCATTGGAAGGCATGCTTTTGGTGATCAGTACCGTGCCACTGATGCTG
TAATCAAGGGACCAGGAAAACTGACTATGACCTTTGgaactgtatttagagagccaattatctg
caaaaatgttcccaagcttgtcccaggtaattattgacaatggcctagttgcattaacaatttt
attttcaa

						Assembled circRNA sequence  
						
							NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							NA						
					
						Genomic sequence
						
							GAACTGTATTTAGAGAGCCAATTATCTGCAAAAATGTTCCCAAGCTTGTCCCAGGTAATTATTG
ACAATGGCCTAGTTGCATTAACAATTTTATTTTCAAAGGGTTCTGAGAATATGTCTTGTTTTCT
CTAGGCTGGACAAAGCCCATCTGCATTGGAAGGCATGCTTTTGGTGATCAGTACCGTGCCACTG
ATGCTGTAATCAAGGGACCAGGAAAACTGACTATGACCTTTG

Sequences
Splice junction sequence	TGGACAAAGCCCATCTGCATTGGAAGGCATGCTTTTGGTGATCAGTACCGTGCCACTGATGCTG TAATCAAGGGACCAGGAAAACTGACTATGACCTTTGgaactgtatttagagagccaattatctg caaaaatgttcccaagcttgtcccaggtaattattgacaatggcctagttgcattaacaatttt attttcaa
Assembled circRNA sequence	NA The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	NA
Genomic sequence	GAACTGTATTTAGAGAGCCAATTATCTGCAAAAATGTTCCCAAGCTTGTCCCAGGTAATTATTG ACAATGGCCTAGTTGCATTAACAATTTTATTTTCAAAGGGTTCTGAGAATATGTCTTGTTTTCT CTAGGCTGGACAAAGCCCATCTGCATTGGAAGGCATGCTTTTGGTGATCAGTACCGTGCCACTG ATGCTGTAATCAAGGGACCAGGAAAACTGACTATGACCTTTG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.346589175

Conservation Information
Conserved circRNAs	NA
PMCS	0.346589175

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							24540331-24540337(+)
24540271-24540109(-)
						
						Potential amino acid sequence
						
							MTFGTVFREPIICKNVPKLVPGWTKPICIGRHAFGDQYRATDAVIKGPGKLTMTFGTVFREPII
CKNVPKLVPGWTKPICIGRHAFGDQYRATDAVIKGPGKLTMTFGTVFREPIICKNVPKLVPGWT
KPICIGRHAFGDQYRATDAVIKGPGKLTMTF(+)
MPSNADGLCPAWDKLGNIFADNWLSKYSSKGHSQFSWSLDYSISGTVLITKSMPSNADGLCPAW
DKLGNIFADNWLSKYSSKGHSQFSWSLDYSISGTVLITKSMPSNADGLCPAWDKLGNIFADNWL
SKYSSKGHSQFSWSLDYSISGTVLITKSMPSNADGLCPAWDKLGNIFADNWLSKYS(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	24540331-24540337(+) 24540271-24540109(-)
Potential amino acid sequence	MTFGTVFREPIICKNVPKLVPGWTKPICIGRHAFGDQYRATDAVIKGPGKLTMTFGTVFREPII CKNVPKLVPGWTKPICIGRHAFGDQYRATDAVIKGPGKLTMTFGTVFREPIICKNVPKLVPGWT KPICIGRHAFGDQYRATDAVIKGPGKLTMTF(+) MPSNADGLCPAWDKLGNIFADNWLSKYSSKGHSQFSWSLDYSISGTVLITKSMPSNADGLCPAW DKLGNIFADNWLSKYSSKGHSQFSWSLDYSISGTVLITKSMPSNADGLCPAWDKLGNIFADNWL SKYSSKGHSQFSWSLDYSISGTVLITKSMPSNADGLCPAWDKLGNIFADNWLSKYS(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of AT1G65930_circ_g.6

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