Plant Circular RNA Database

Detail information of Csa3G821030_circ_g.4

			
						 General Information
					
						CircRNA Name
						Csa3G821030_circ_g.4
					
						ID in PlantcircBase
						csa_circ_002053
					
						Alias
						Chr3:31961286_31967697
					
						Organism
						
							Cucumis sativus						
					
						Position
						
							chrChr3: 31961286-31967697  JBrowse»						
					
						Reference genome
						
							Cucumis sativus v1.5						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI2;Find_circ
					
						Parent gene
						
							Csa3G821030						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							Csa3G821030_circ_g.8 						
					
						Support reads
						NA
					
						Tissues
						leaf,root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Csa3G821030.1:7

General Information
CircRNA Name	Csa3G821030_circ_g.4
ID in PlantcircBase	csa_circ_002053
Alias	Chr3:31961286_31967697
Organism	Cucumis sativus
Position	chrChr3: 31961286-31967697 JBrowse»
Reference genome	Cucumis sativus v1.5
Type	e-circRNA
Identification method	CIRI2;Find_circ
Parent gene	Csa3G821030
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	Csa3G821030_circ_g.8
Support reads	NA
Tissues	leaf,root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Csa3G821030.1:7

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CGTATGGAAAGTGGATCAAGGAAAATATGCGCTCATTGAAGGCTGAGAATTTTCTTGCATCAAC
AGTATTTGAAACGGATAAATTGCTGAGAAGCCAGCAttgttgcaagtatctttagacaagaagg
tcttgaggtgcttggttggaggcctgttcctgtgaaagcttctgtggttggtataaatgcaaag
aagaccat

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 1081
Transcript exons: 31961286-31961728,31961863-31961994,31962726-31962824,31
963307-31963377,31963662-31963758,31964366-31964458,3196
7552-31967697
TTGTTGCAAGTATCTTTAGACAAGAAGGTCTTGAGGTGCTTGGTTGGAGGCCTGTTCCTGTGAA
AGCTTCTGTGGTTGGTATAAATGCAAAGAAGACCATGCCTAACATTGAGCAGGTATTCGTCCAA
GTAGTTAAAGAAGAGAATGTGGACGACATTGAACGAGAACTTTACATTTGTAGGAAATTGATTG
AAAGAGAAGCAAACTCTAAGAGTTGGGGAAGTGAGCTATATTTCTGTTCTTTGTCAAACCAAAC
TATAGTTTACAAGGGAATGCTTCGCTCAGAGGTTCTCGGATTATTTTATGATGACCTTCAGAAT
GATCTCTATAAATCCCCCTTTGCTATTTATCATCGAAGATATAGTACAAATACTAGTCCTCGAT
GGCCTCTTGCCCAACCAATGAGGTTACTGGGTCATAATGGAGAGATCAATACGATACAGGGAAA
CTTAAATTGGATGCAATCTCGAGAAGCTTCCTTAAAGTCATCTGTATGGCGGGGTCGTGAAAAT
GAAATTCGTCCATATGGCAACCCAAGGGCATCGGATTCAGCAAATCTTGATAGTGCAGCCGAAT
TGTTAATAAGAAGTGGCCGTGCCCCTGAGGAGGCTCTCATGATCCTTGTTCCTGAGGCTTACAA
GAATCATCCTACATTGATGATCAAATATCCAGAGGTTGTTGACTTTTATGACTATTACAAAGGT
CAAATGGAGGCTTGGGATGGACCTGCTTTATTGTTGTTCAGCGATGGAAAAACAGTTGGTGCCT
GTCTTGATCGCAATGGATTGCGCCCAGCTCGGTATTGGCGCACGTCAGATAATTTTGTTTATGT
TGCATCTGAGGTTGGCGTCTTACCAATGGATGAGTCTAAAGTTACAATGAAAGGCCGTCTTGGT
CCAGGAATGATGATTGCTGCTGATTTGCAGACCGGACAGGTTTATGAGAATACAGAAGTTAAAA
AACGTGTAGCGTTGTCATATCCGTATGGAAAGTGGATCAAGGAAAATATGCGCTCATTGAAGGC
TGAGAATTTTCTTGCATCAACAGTATTTGAAACGGATAAATTGCTGAGAAGCCAGCA
						
						Genomic sequence
						
							TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

Sequences
Splice junction sequence	CGTATGGAAAGTGGATCAAGGAAAATATGCGCTCATTGAAGGCTGAGAATTTTCTTGCATCAAC AGTATTTGAAACGGATAAATTGCTGAGAAGCCAGCAttgttgcaagtatctttagacaagaagg tcttgaggtgcttggttggaggcctgttcctgtgaaagcttctgtggttggtataaatgcaaag aagaccat
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 1081 Transcript exons: 31961286-31961728,31961863-31961994,31962726-31962824,31 963307-31963377,31963662-31963758,31964366-31964458,3196 7552-31967697 TTGTTGCAAGTATCTTTAGACAAGAAGGTCTTGAGGTGCTTGGTTGGAGGCCTGTTCCTGTGAA AGCTTCTGTGGTTGGTATAAATGCAAAGAAGACCATGCCTAACATTGAGCAGGTATTCGTCCAA GTAGTTAAAGAAGAGAATGTGGACGACATTGAACGAGAACTTTACATTTGTAGGAAATTGATTG AAAGAGAAGCAAACTCTAAGAGTTGGGGAAGTGAGCTATATTTCTGTTCTTTGTCAAACCAAAC TATAGTTTACAAGGGAATGCTTCGCTCAGAGGTTCTCGGATTATTTTATGATGACCTTCAGAAT GATCTCTATAAATCCCCCTTTGCTATTTATCATCGAAGATATAGTACAAATACTAGTCCTCGAT GGCCTCTTGCCCAACCAATGAGGTTACTGGGTCATAATGGAGAGATCAATACGATACAGGGAAA CTTAAATTGGATGCAATCTCGAGAAGCTTCCTTAAAGTCATCTGTATGGCGGGGTCGTGAAAAT GAAATTCGTCCATATGGCAACCCAAGGGCATCGGATTCAGCAAATCTTGATAGTGCAGCCGAAT TGTTAATAAGAAGTGGCCGTGCCCCTGAGGAGGCTCTCATGATCCTTGTTCCTGAGGCTTACAA GAATCATCCTACATTGATGATCAAATATCCAGAGGTTGTTGACTTTTATGACTATTACAAAGGT CAAATGGAGGCTTGGGATGGACCTGCTTTATTGTTGTTCAGCGATGGAAAAACAGTTGGTGCCT GTCTTGATCGCAATGGATTGCGCCCAGCTCGGTATTGGCGCACGTCAGATAATTTTGTTTATGT TGCATCTGAGGTTGGCGTCTTACCAATGGATGAGTCTAAAGTTACAATGAAAGGCCGTCTTGGT CCAGGAATGATGATTGCTGCTGATTTGCAGACCGGACAGGTTTATGAGAATACAGAAGTTAAAA AACGTGTAGCGTTGTCATATCCGTATGGAAAGTGGATCAAGGAAAATATGCGCTCATTGAAGGC TGAGAATTTTCTTGCATCAACAGTATTTGAAACGGATAAATTGCTGAGAAGCCAGCA
Genomic sequence	TOO LONG (>5KB) TO SHOW GENOMIC SEQUENCE OF THIS CIRCULAR RNA. PLEASE FIND THE CORRESPONDING GENOMIC SEQUENCE AT DOWNLOAD SECTION.

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.995049984

Conservation Information
Conserved circRNAs	NA
PMCS	0.995049984

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							31961385-31961301(+)
						
						Potential amino acid sequence
						
							MPNIEQVFVQVVKEENVDDIERELYICRKLIEREANSKSWGSELYFCSLSNQTIVYKGMLRSEV
LGLFYDDLQNDLYKSPFAIYHRRYSTNTSPRWPLAQPMRLLGHNGEINTIQGNLNWMQSREASL
KSSVWRGRENEIRPYGNPRASDSANLDSAAELLIRSGRAPEEALMILVPEAYKNHPTLMIKYPE
VVDFYDYYKGQMEAWDGPALLLFSDGKTVGACLDRNGLRPARYWRTSDNFVYVASEVGVLPMDE
SKVTMKGRLGPGMMIAADLQTGQVYENTEVKKRVALSYPYGKWIKENMRSLKAENFLASTVFET
DKLLRSQHCCKYL*
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to salt stress in root

Functional Information
Coding potential	Y
Potential coding position	31961385-31961301(+)
Potential amino acid sequence	MPNIEQVFVQVVKEENVDDIERELYICRKLIEREANSKSWGSELYFCSLSNQTIVYKGMLRSEV LGLFYDDLQNDLYKSPFAIYHRRYSTNTSPRWPLAQPMRLLGHNGEINTIQGNLNWMQSREASL KSSVWRGRENEIRPYGNPRASDSANLDSAAELLIRSGRAPEEALMILVPEAYKNHPTLMIKYPE VVDFYDYYKGQMEAWDGPALLLFSDGKTVGACLDRNGLRPARYWRTSDNFVYVASEVGVLPMDE SKVTMKGRLGPGMMIAADLQTGQVYENTEVKKRVALSYPYGKWIKENMRSLKAENFLASTVFET DKLLRSQHCCKYL*
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to salt stress in root

			
						 Other Information
					
						References
						Zhu et al., 2019;He et al., 2020

circRNA

Detail information of Csa3G821030_circ_g.4

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