Plant Circular RNA Database

Detail information of orai.002G118000_circ_g.1

			
						 General Information
					
						CircRNA Name
						orai.002G118000_circ_g.1
					
						ID in PlantcircBase
						gra_circ_000148
					
						Alias
						Chr02:16753013|16754020
					
						Organism
						
							Gossypium raimondii						
					
						Position
						
							chrChr02: 16753013-16754020  JBrowse»						
					
						Reference genome
						
							Graimondii_221						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRI
					
						Parent gene
						
							Gorai.002G118000						
					
						Parent gene annotation
						
							NA							
					
						Parent gene strand
						+
					
						Alternative splicing
						
							NA						
					
						Support reads
						3
					
						Tissues
						ovule
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Gorai.002G118000.3:3
Gorai.002G118000.5:3
Gorai.002G118000.1:3
Gorai.002G118000.4:3
Gorai.002G118000.2:3

General Information
CircRNA Name	orai.002G118000_circ_g.1
ID in PlantcircBase	gra_circ_000148
Alias	Chr02:16753013\|16754020
Organism	Gossypium raimondii
Position	chrChr02: 16753013-16754020 JBrowse»
Reference genome	Graimondii_221
Type	e-circRNA
Identification method	CIRI
Parent gene	Gorai.002G118000
Parent gene annotation	NA
Parent gene strand	+
Alternative splicing	NA
Support reads	3
Tissues	ovule
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Gorai.002G118000.3:3 Gorai.002G118000.5:3 Gorai.002G118000.1:3 Gorai.002G118000.4:3 Gorai.002G118000.2:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							TGCAAAGATGAAAAGCATAATCCATGCTAATCAATGTTTAGATTTGATGGAGCTGCAAAGGGTT
GGGAAAAATGAAATTACATATTCAGTGCTTCTAAAGgtaatgtgtttttgggatatggaggaga
gccattaacaaagtccattcaagtgcaaattgttgatgcccttcgcttaggtgagagaagtaga
gcttctag

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 485
Transcript exons: 16753013-16753206,16753406-16753513,16753838-16754020
GTAATGTGTTTTTGGGATATGGAGGAGAGCCATTAACAAAGTCCATTCAAGTGCAAATTGTTGA
TGCCCTTCGCTTAGGTGAGAGAAGTAGAGCTTCTAGCCTGCTTTTAGACTTTGGTAACGGAAAC
CAGTCTTTAAAAGCCAATGACTTCGTTTACATTCTTAACTACTGTGCAAGGTCACCGGATCCTT
TGTTCGTTATGGAAACATGGAGATTAATGGAGGAGAAGGAGATTGACTTGAATAATACATGCTA
TTTGCTTATGGTCCGAGCTCTTTGTAGAGGAGGTTACTTGGAGGAGGCATGCAAGTTCATGAAA
TTCCTTAGGGAGAATCATGGCACCTATCCTCTTCTTCCCGTATACAACTGTTTCTTAGGAGCTT
GTGCAAAGATGAAAAGCATAATCCATGCTAATCAATGTTTAGATTTGATGGAGCTGCAAAGGGT
TGGGAAAAATGAAATTACATATTCAGTGCTTCTAAAG
						
						Genomic sequence
						
							GTAATGTGTTTTTGGGATATGGAGGAGAGCCATTAACAAAGTCCATTCAAGTGCAAATTGTTGA
TGCCCTTCGCTTAGGTGAGAGAAGTAGAGCTTCTAGCCTGCTTTTAGACTTTGGTAACGGAAAC
CAGTCTTTAAAAGCCAATGACTTCGTTTACATTCTTAACTACTGTGCAAGGTCACCGGATCCTT
TGGTAAGCTGTTCAACGTGGAATTTTAGCTAGCTGCATGCAACTGTGTAAACCTCAATGTGTTT
CTCTTCTCTAGATTTAAGCTTAGGACATAGATAATGTGGATATTTATAATATTTGACAAACTGG
AAAAGGAAGAAAAGATAGCATCAATGGTTATACTTGGCAGTAACAGTTGTGATTTATTTCCTGT
CCTGTACAGTTCGTTATGGAAACATGGAGATTAATGGAGGAGAAGGAGATTGACTTGAATAATA
CATGCTATTTGCTTATGGTCCGAGCTCTTTGTAGAGGAGGTTACTTGGAGGAGGTATGCCTTTT
ATCTACTGGTTTTGTTAGATGTTCTGTTTGTTTAACTATTAAACACTGATCTTCTAAATGTATT
ACCTCAGTGTATCTTTGGGTGTTGATCCATTTAACTTAACTAGAACAACCTACAACCTTTTCTC
TTGTGTAATTGTGAACAATCTTTTGTCATTGCTGCATTGTTGTACTTATAGTTGTTCTGTGTAG
TCATTAATGTTATTATTGTCCTGAATCACATTAAAATGTATGTAGATGGATGCTTATACAGTTC
CTCTTCCTCAATTTCCATTACAAGTTTTCTCATTTCTTCTTGTTTTATTTCCATCAGGCATGCA
AGTTCATGAAATTCCTTAGGGAGAATCATGGCACCTATCCTCTTCTTCCCGTATACAACTGTTT
CTTAGGAGCTTGTGCAAAGATGAAAAGCATAATCCATGCTAATCAATGTTTAGATTTGATGGAG
CTGCAAAGGGTTGGGAAAAATGAAATTACATATTCAGTGCTTCTAAAG

Sequences
Splice junction sequence	TGCAAAGATGAAAAGCATAATCCATGCTAATCAATGTTTAGATTTGATGGAGCTGCAAAGGGTT GGGAAAAATGAAATTACATATTCAGTGCTTCTAAAGgtaatgtgtttttgggatatggaggaga gccattaacaaagtccattcaagtgcaaattgttgatgcccttcgcttaggtgagagaagtaga gcttctag
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 485 Transcript exons: 16753013-16753206,16753406-16753513,16753838-16754020 GTAATGTGTTTTTGGGATATGGAGGAGAGCCATTAACAAAGTCCATTCAAGTGCAAATTGTTGA TGCCCTTCGCTTAGGTGAGAGAAGTAGAGCTTCTAGCCTGCTTTTAGACTTTGGTAACGGAAAC CAGTCTTTAAAAGCCAATGACTTCGTTTACATTCTTAACTACTGTGCAAGGTCACCGGATCCTT TGTTCGTTATGGAAACATGGAGATTAATGGAGGAGAAGGAGATTGACTTGAATAATACATGCTA TTTGCTTATGGTCCGAGCTCTTTGTAGAGGAGGTTACTTGGAGGAGGCATGCAAGTTCATGAAA TTCCTTAGGGAGAATCATGGCACCTATCCTCTTCTTCCCGTATACAACTGTTTCTTAGGAGCTT GTGCAAAGATGAAAAGCATAATCCATGCTAATCAATGTTTAGATTTGATGGAGCTGCAAAGGGT TGGGAAAAATGAAATTACATATTCAGTGCTTCTAAAG
Genomic sequence	GTAATGTGTTTTTGGGATATGGAGGAGAGCCATTAACAAAGTCCATTCAAGTGCAAATTGTTGA TGCCCTTCGCTTAGGTGAGAGAAGTAGAGCTTCTAGCCTGCTTTTAGACTTTGGTAACGGAAAC CAGTCTTTAAAAGCCAATGACTTCGTTTACATTCTTAACTACTGTGCAAGGTCACCGGATCCTT TGGTAAGCTGTTCAACGTGGAATTTTAGCTAGCTGCATGCAACTGTGTAAACCTCAATGTGTTT CTCTTCTCTAGATTTAAGCTTAGGACATAGATAATGTGGATATTTATAATATTTGACAAACTGG AAAAGGAAGAAAAGATAGCATCAATGGTTATACTTGGCAGTAACAGTTGTGATTTATTTCCTGT CCTGTACAGTTCGTTATGGAAACATGGAGATTAATGGAGGAGAAGGAGATTGACTTGAATAATA CATGCTATTTGCTTATGGTCCGAGCTCTTTGTAGAGGAGGTTACTTGGAGGAGGTATGCCTTTT ATCTACTGGTTTTGTTAGATGTTCTGTTTGTTTAACTATTAAACACTGATCTTCTAAATGTATT ACCTCAGTGTATCTTTGGGTGTTGATCCATTTAACTTAACTAGAACAACCTACAACCTTTTCTC TTGTGTAATTGTGAACAATCTTTTGTCATTGCTGCATTGTTGTACTTATAGTTGTTCTGTGTAG TCATTAATGTTATTATTGTCCTGAATCACATTAAAATGTATGTAGATGGATGCTTATACAGTTC CTCTTCCTCAATTTCCATTACAAGTTTTCTCATTTCTTCTTGTTTTATTTCCATCAGGCATGCA AGTTCATGAAATTCCTTAGGGAGAATCATGGCACCTATCCTCTTCTTCCCGTATACAACTGTTT CTTAGGAGCTTGTGCAAAGATGAAAAGCATAATCCATGCTAATCAATGTTTAGATTTGATGGAG CTGCAAAGGGTTGGGAAAAATGAAATTACATATTCAGTGCTTCTAAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.769935078

Conservation Information
Conserved circRNAs	NA
PMCS	0.769935078

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							16753412-16753045(+)
16753059-16754000(-)
						
						Potential amino acid sequence
						
							METWRLMEEKEIDLNNTCYLLMVRALCRGGYLEEACKFMKFLRENHGTYPLLPVYNCFLGACAK
MKSIIHANQCLDLMELQRVGKNEITYSVLLKVMCFWDMEESH*(+)
MDFVNGSPPYPKNTLPLEALNM*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	16753412-16753045(+) 16753059-16754000(-)
Potential amino acid sequence	METWRLMEEKEIDLNNTCYLLMVRALCRGGYLEEACKFMKFLRENHGTYPLLPVYNCFLGACAK MKSIIHANQCLDLMELQRVGKNEITYSVLLKVMCFWDMEESH(+) MDFVNGSPPYPKNTLPLEALNM(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Zhao et al., 2017b

circRNA

Detail information of orai.002G118000_circ_g.1

Address

Any questions?

Supported by