Plant Circular RNA Database

Detail information of Zm00001d014858_circ_g.4

			
						 General Information
					
						CircRNA Name
						Zm00001d014858_circ_g.4
					
						ID in PlantcircBase
						zma_circ_008514
					
						Alias
						Zm05circ00050, GRMZM5G834758_C1
					
						Organism
						
							Zea mays						
					
						Position
						
							chr5: 65681591-65682432  JBrowse»						
					
						Reference genome
						
							AGPv4.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRI2
					
						Parent gene
						
							Zm00001d014858						
					
						Parent gene annotation
						
							DNA-binding protein MNB1B  
							
						Parent gene strand
						-
					
						Alternative splicing
						
							Zm00001d014858_circ_g.2 Zm00001d014858_circ_g.3 						
					
						Support reads
						NA
					
						Tissues
						leaf, endosperm
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						Zm00001d014858_T003:3
Zm00001d014858_T004:3
Zm00001d014858_T001:5
Zm00001d014858_T002:2

General Information
CircRNA Name	Zm00001d014858_circ_g.4
ID in PlantcircBase	zma_circ_008514
Alias	Zm05circ00050, GRMZM5G834758_C1
Organism	Zea mays
Position	chr5: 65681591-65682432 JBrowse»
Reference genome	AGPv4.38
Type	ue-circRNA
Identification method	CIRI2
Parent gene	Zm00001d014858
Parent gene annotation	DNA-binding protein MNB1B
Parent gene strand	-
Alternative splicing	Zm00001d014858_circ_g.2 Zm00001d014858_circ_g.3
Support reads	NA
Tissues	leaf, endosperm
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	Zm00001d014858_T003:3 Zm00001d014858_T004:3 Zm00001d014858_T001:5 Zm00001d014858_T002:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GAAAGCGCTGGGAGCCCTCTTAGGCTTGTTGGGGTCCTTGCCGGCCTTCCCTTTCCTGCCCTTG
GCGGGCTTCTCCGCCCCCTTGCTCTTCACCGCCAGCctcctcactaccctcttcatcatcctcg
tcattgacctccgacttggacttgtcagactcctcttcatcttcctcctcttcctccttggcag
gagccttc

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 409
Transcript exons: 65681591-65681704,65681816-65681896,65681991-65682035,65
682164-65682221,65682322-65682432
CTCCTCACTACCCTCTTCATCATCCTCGTCATTGACCTCCGACTTGGACTTGTCAGACTCCTCT
TCATCTTCCTCCTCTTCCTCCTTGGCAGGAGCCTTCTTAGCTGCAGTGCTCTCGCCCTTGTTGT
AGGCAGCGATGGCCTTGTTGTACTCGAGCTTGAGCTTGTTAGCCTTGGCTACATAGGGAGCCTT
GTCCGACTCGCTCAGGGATTTCCACCTGTCGCCAGCAGCTTTCCCCACCGCAGCGACAGACTTG
TTCTTAGGGTTCTTCTCCTTGAACTCCTTGCGAAATTCCTCCATGAACACGAAGAAAGCGCTGG
GAGCCCTCTTAGGCTTGTTGGGGTCCTTGCCGGCCTTCCCTTTCCTGCCCTTGGCGGGCTTCTC
CGCCCCCTTGCTCTTCACCGCCAGC
						
						Genomic sequence
						
							CTCCTCACTACCCTCTTCATCATCCTCGTCATTGACCTCCGACTTGGACTTGTCAGACTCCTCT
TCATCTTCCTCCTCTTCCTCCTTGGCAGGAGCCTTCTTAGCTGCAGTGCTCTGAACAAACAACA
CATGGTTCAGTAAGCATACGTATTTGAATAAATGACCAGCAGGAGCAGTCTATATACACATTAA
GTAACAACGCAATTGAAGCATCAGTTTCCCTACCTCGCCCTTGTTGTAGGCAGCGATGGCCTTG
TTGTACTCGAGCTTGAGCTTGTTAGCCTTGGCTACATAGGGAGCCTTGTCCTACAATCCACACA
AACGCACGCGTAAAACAATTAATCAGCACGAGATTTCTAGTCCAATCAACTACTACTTTAGTAC
AGAAGATGGAGCTTACCGACTCGCTCAGGGATTTCCACCTGTCGCCAGCAGCTTTCCCCACCTG
AAAAGTTCGACCAGGAGAGGGGAATCGTGACGAGTAAATTAAATAATAAGTCGTCATTAAGGGA
AAAGTCAAAGTAGATAGTAATAATATGGGGCCGGTCATAAAGCAGCTCTCAGGGTTCTTACCGC
AGCGACAGACTTGTTCTTAGGGTTCTTCTCCTTGAACTCCTTGCGAAATTCCTCCCTGTATAAA
AAGGCATCCAAAAATAGATCGAATCAACAGTCGCATCCAGAAATAAGTATATAGCAAAAAAAAA
GAGACGACGAAAAAAGTCGCATCTCACATGAACACGAAGAAAGCGCTGGGAGCCCTCTTAGGCT
TGTTGGGGTCCTTGCCGGCCTTCCCTTTCCTGCCCTTGGCGGGCTTCTCCGCCCCCTTGCTCTT
CACCGCCAGC

Sequences
Splice junction sequence	GAAAGCGCTGGGAGCCCTCTTAGGCTTGTTGGGGTCCTTGCCGGCCTTCCCTTTCCTGCCCTTG GCGGGCTTCTCCGCCCCCTTGCTCTTCACCGCCAGCctcctcactaccctcttcatcatcctcg tcattgacctccgacttggacttgtcagactcctcttcatcttcctcctcttcctccttggcag gagccttc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 409 Transcript exons: 65681591-65681704,65681816-65681896,65681991-65682035,65 682164-65682221,65682322-65682432 CTCCTCACTACCCTCTTCATCATCCTCGTCATTGACCTCCGACTTGGACTTGTCAGACTCCTCT TCATCTTCCTCCTCTTCCTCCTTGGCAGGAGCCTTCTTAGCTGCAGTGCTCTCGCCCTTGTTGT AGGCAGCGATGGCCTTGTTGTACTCGAGCTTGAGCTTGTTAGCCTTGGCTACATAGGGAGCCTT GTCCGACTCGCTCAGGGATTTCCACCTGTCGCCAGCAGCTTTCCCCACCGCAGCGACAGACTTG TTCTTAGGGTTCTTCTCCTTGAACTCCTTGCGAAATTCCTCCATGAACACGAAGAAAGCGCTGG GAGCCCTCTTAGGCTTGTTGGGGTCCTTGCCGGCCTTCCCTTTCCTGCCCTTGGCGGGCTTCTC CGCCCCCTTGCTCTTCACCGCCAGC
Genomic sequence	CTCCTCACTACCCTCTTCATCATCCTCGTCATTGACCTCCGACTTGGACTTGTCAGACTCCTCT TCATCTTCCTCCTCTTCCTCCTTGGCAGGAGCCTTCTTAGCTGCAGTGCTCTGAACAAACAACA CATGGTTCAGTAAGCATACGTATTTGAATAAATGACCAGCAGGAGCAGTCTATATACACATTAA GTAACAACGCAATTGAAGCATCAGTTTCCCTACCTCGCCCTTGTTGTAGGCAGCGATGGCCTTG TTGTACTCGAGCTTGAGCTTGTTAGCCTTGGCTACATAGGGAGCCTTGTCCTACAATCCACACA AACGCACGCGTAAAACAATTAATCAGCACGAGATTTCTAGTCCAATCAACTACTACTTTAGTAC AGAAGATGGAGCTTACCGACTCGCTCAGGGATTTCCACCTGTCGCCAGCAGCTTTCCCCACCTG AAAAGTTCGACCAGGAGAGGGGAATCGTGACGAGTAAATTAAATAATAAGTCGTCATTAAGGGA AAAGTCAAAGTAGATAGTAATAATATGGGGCCGGTCATAAAGCAGCTCTCAGGGTTCTTACCGC AGCGACAGACTTGTTCTTAGGGTTCTTCTCCTTGAACTCCTTGCGAAATTCCTCCCTGTATAAA AAGGCATCCAAAAATAGATCGAATCAACAGTCGCATCCAGAAATAAGTATATAGCAAAAAAAAA GAGACGACGAAAAAAGTCGCATCTCACATGAACACGAAGAAAGCGCTGGGAGCCCTCTTAGGCT TGTTGGGGTCCTTGCCGGCCTTCCCTTTCCTGCCCTTGGCGGGCTTCTCCGCCCCCTTGCTCTT CACCGCCAGC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.124439861

Conservation Information
Conserved circRNAs	NA
PMCS	0.124439861

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							65682323-65681622(+)
65682325-65682354(-)
65681660-65682354(-)
						
						Potential amino acid sequence
						
							MNTKKALGALLGLLGSLPAFPFLPLAGFSAPLLFTASLLTTLFIILVIDLRLGLVRLLFIFLLF
LLGRSLLSCSALALVVGSDGLVVLELELVSLGYIGSLVRLAQGFPPVASSFPHRSDRLVLRVLL
LELLAKFLHEHEESAGSPLRLVGVLAGLPFPALGGLLRPLALHRQPPHYPLHHPRH*(+)
MEEFRKEFKEKNPKNKSVAAVGKAAGDRWKSLSESDKAPYVAKANKLKLEYNKAIAAYNKGEST
AAKKAPAKEEEEEDEEESDKSKSEVNDEDDEEGSEEAGGEEQGGGEARQGQEREGRQGPQQA*(
-)
MKRSLTSPSRRSMTRMMKRVVRRLAVKSKGAEKPAKGRKGKAGKDPNKPKRAPSAFFVFMEEFR
KEFKEKNPKNKSVAAVGKAAGDRWKSLSESDKAPYVAKANKLKLEYNKAIAAYNKGESTAAKKA
PAKEEEEEDEEESDKSKSEVNDEDDEEGSEEAGGEEQGGGEARQGQEREGRQGPQQA*(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						responsive to drought stress

Functional Information
Coding potential	Y
Potential coding position	65682323-65681622(+) 65682325-65682354(-) 65681660-65682354(-)
Potential amino acid sequence	MNTKKALGALLGLLGSLPAFPFLPLAGFSAPLLFTASLLTTLFIILVIDLRLGLVRLLFIFLLF LLGRSLLSCSALALVVGSDGLVVLELELVSLGYIGSLVRLAQGFPPVASSFPHRSDRLVLRVLL LELLAKFLHEHEESAGSPLRLVGVLAGLPFPALGGLLRPLALHRQPPHYPLHHPRH(+) MEEFRKEFKEKNPKNKSVAAVGKAAGDRWKSLSESDKAPYVAKANKLKLEYNKAIAAYNKGEST AAKKAPAKEEEEEDEEESDKSKSEVNDEDDEEGSEEAGGEEQGGGEARQGQEREGRQGPQQA( -) MKRSLTSPSRRSMTRMMKRVVRRLAVKSKGAEKPAKGRKGKAGKDPNKPKRAPSAFFVFMEEFR KEFKEKNPKNKSVAAVGKAAGDRWKSLSESDKAPYVAKANKLKLEYNKAIAAYNKGESTAAKKA PAKEEEEEDEEESDKSKSEVNDEDDEEGSEEAGGEEQGGGEARQGQEREGRQGPQQA*(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	responsive to drought stress

			
						 Other Information
					
						References
						Han et al., 2020;Zhang et al., 2019

circRNA

Detail information of Zm00001d014858_circ_g.4

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