Plant Circular RNA Database

Detail information of AT2G32415_circ_g.2

			
						 General Information
					
						CircRNA Name
						AT2G32415_circ_g.2
					
						ID in PlantcircBase
						ath_circ_016087
					
						Alias
						NA
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr2: 13761559-13762751  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, KNIFE, PcircRNA_finder
					
						Parent gene
						
							AT2G32415						
					
						Parent gene annotation
						
							Protein RRP6-like 3
							
						Parent gene strand
						-
					
						Alternative splicing
						
							AT2G32415_circ_g.1 						
					
						Support reads
						2
					
						Tissues
						inflorescences, whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							CT-AC							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT2G32415.2:3
AT2G32415.1:3
AT2G32415.3:3

General Information
CircRNA Name	AT2G32415_circ_g.2
ID in PlantcircBase	ath_circ_016087
Alias	NA
Organism	Arabidpsis thaliana
Position	chr2: 13761559-13762751 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	CIRCexplorer, KNIFE, PcircRNA_finder
Parent gene	AT2G32415
Parent gene annotation	Protein RRP6-like 3
Parent gene strand	-
Alternative splicing	AT2G32415_circ_g.1
Support reads	2
Tissues	inflorescences, whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	CT-AC The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT2G32415.2:3 AT2G32415.1:3 AT2G32415.3:3

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
				
						 Sequences
					

				
						Splice junction sequence  
						
							ATCGTTCCCCTCATCTTCTGGACGCCCTTTGGGCTCAAACAAAAGCATTATCGCAGGTGGATTT
TCTTCAACTAGCTTCGCAAGGCCACGGTTTAGATACctgtgactaacattccaaccaccaggta
aatggcgaggatgaacagcatcaacaaacacttttctccacctttgacagaaatcacgtacacc
atcttctc

									
								

					

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

					

						Full-length trsnacripts
						
							Transcript length: 996
Transcript exons: 13761559-13761889,13761995-13762154,13762247-13762751
CTGTGACTAACATTCCAACCACCAGGTAAATGGCGAGGATGAACAGCATCAACAAACACTTTTC
TCCACCTTTGACAGAAATCACGTACACCATCTTCTCCATGTTCCCTTAAAAGATATTCTACGAT
CTGTTTCCCGTGCGGTCCATGTCCTAACAAAGAGAGTTTGGAGTTTTGTTGGTGCAACGTATTC
CCATTACACTGAGTATCATTTAGCTCTTTAGCTCCATCTCCTTCACTTGTACCATCACCACTAT
CATCAACAACTGTGCTCCTTTCTACAGTCATAATTTTCTCAAAATCTGCAAGAGCCTCCCCATC
ATTGTTTTCATCTTCTTGTTTGTCCATACCAGCAACCTCTGCAGAATGTTTGAAGGAGACCCCT
TTTTTCCTTTCGAGTTTTCTTCTTTCGTGAGGGCTCAATCCGATGAGTAAAGCTTTCTCTAAAT
CTTCTTCAGATAGGTCCCGTCCACCGTAATACATCTTCACAGTCTGCAACAATTCCTCACGGCG
ACTTGATGGCATTCTGTTCCCATGTCGCAAGAGCGCCATTGCAGCTGATCTAAGATGTAATGGG
GAGACACCTGCATCCTCAGAGTCACCAGTTGATTCATCCTCCACTAAAGAAGACGTCCCTTGTG
CTTCTTTTGAATCGAGTACTCTACGAACAAAAAGAGGAATTCCAAATTCCGTAGCGATTTGTTT
CTTGTACCTCTCTGCAGCAGCATGTGCAACTTCATGACAATCCACACAAAGAAGAACAATGTCA
TGAGATCTATGACTTTTTAGGTGCTCAGGAAAATGAACTCTATAGCACGACGGTATTATTCGGT
AACGGAGATAATGTTTCCCTTCCCCACACCCAACACATATATTCCTCTTGGTCTGGATATAAAA
ATCGTTCCCCTCATCTTCTGGACGCCCTTTGGGCTCAAACAAAAGCATTATCGCAGGTGGATTT
TCTTCAACTAGCTTCGCAAGGCCACGGTTTAGATAC
						
					

						Genomic sequence
						
							CTGTGACTAACATTCCAACCACCAGGTAAATGGCGAGGATGAACAGCATCAACAAACACTTTTC
TCCACCTTTGACAGAAATCACGTACACCATCTTCTCCATGTTCCCTTAAAAGATATTCTACGAT
CTGTTTCCCGTGCGGTCCATGTCCTAACAAAGAGAGTTTGGAGTTTTGTTGGTGCAACGTATTC
CCATTACACTGAGTATCATTTAGCTCTTTAGCTCCATCTCCTTCACTTGTACCATCACCACTAT
CATCAACAACTGTGCTCCTTTCTACAGTCATAATTTTCTCAAAATCTGCAAGAGCCTCCCCATC
ATTGTTTTCATCTAAAGATACGAAGTTGTTTGCATCAGTATTTCTGGATATCAAAGGATCATCA
TATCTTACACTTTATCAACCAACACAATTTTCAATGCTTATTATATGGTTACCTTCTTGTTTGT
CCATACCAGCAACCTCTGCAGAATGTTTGAAGGAGACCCCTTTTTTCCTTTCGAGTTTTCTTCT
TTCGTGAGGGCTCAATCCGATGAGTAAAGCTTTCTCTAAATCTTCTTCAGATAGGTCCCGTCCA
CCGTAATACATCTTCACAGTCTATATTCAGAATCCAAAAGAAAGTATCAATAACAGAATACTTG
GTTCAAATCAATTTCTTGAAAGTTGAAATAGTTAGATGATCACATCACCTGCAACAATTCCTCA
CGGCGACTTGATGGCATTCTGTTCCCATGTCGCAAGAGCGCCATTGCAGCTGATCTAAGATGTA
ATGGGGAGACACCTGCATCCTCAGAGTCACCAGTTGATTCATCCTCCACTAAAGAAGACGTCCC
TTGTGCTTCTTTTGAATCGAGTACTCTACGAACAAAAAGAGGAATTCCAAATTCCGTAGCGATT
TGTTTCTTGTACCTCTCTGCAGCAGCATGTGCAACTTCATGACAATCCACACAAAGAAGAACAA
TGTCATGAGATCTATGACTTTTTAGGTGCTCAGGAAAATGAACTCTATAGCACGACGGTATTAT
TCGGTAACGGAGATAATGTTTCCCTTCCCCACACCCAACACATATATTCCTCTTGGTCTGGATA
TAAAAATCGTTCCCCTCATCTTCTGGACGCCCTTTGGGCTCAAACAAAAGCATTATCGCAGGTG
GATTTTCTTCAACTAGCTTCGCAAGGCCACGGTTTAGATAC
						
					

			
		

Sequences
Splice junction sequence	ATCGTTCCCCTCATCTTCTGGACGCCCTTTGGGCTCAAACAAAAGCATTATCGCAGGTGGATTT TCTTCAACTAGCTTCGCAAGGCCACGGTTTAGATACctgtgactaacattccaaccaccaggta aatggcgaggatgaacagcatcaacaaacacttttctccacctttgacagaaatcacgtacacc atcttctc
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 996 Transcript exons: 13761559-13761889,13761995-13762154,13762247-13762751 CTGTGACTAACATTCCAACCACCAGGTAAATGGCGAGGATGAACAGCATCAACAAACACTTTTC TCCACCTTTGACAGAAATCACGTACACCATCTTCTCCATGTTCCCTTAAAAGATATTCTACGAT CTGTTTCCCGTGCGGTCCATGTCCTAACAAAGAGAGTTTGGAGTTTTGTTGGTGCAACGTATTC CCATTACACTGAGTATCATTTAGCTCTTTAGCTCCATCTCCTTCACTTGTACCATCACCACTAT CATCAACAACTGTGCTCCTTTCTACAGTCATAATTTTCTCAAAATCTGCAAGAGCCTCCCCATC ATTGTTTTCATCTTCTTGTTTGTCCATACCAGCAACCTCTGCAGAATGTTTGAAGGAGACCCCT TTTTTCCTTTCGAGTTTTCTTCTTTCGTGAGGGCTCAATCCGATGAGTAAAGCTTTCTCTAAAT CTTCTTCAGATAGGTCCCGTCCACCGTAATACATCTTCACAGTCTGCAACAATTCCTCACGGCG ACTTGATGGCATTCTGTTCCCATGTCGCAAGAGCGCCATTGCAGCTGATCTAAGATGTAATGGG GAGACACCTGCATCCTCAGAGTCACCAGTTGATTCATCCTCCACTAAAGAAGACGTCCCTTGTG CTTCTTTTGAATCGAGTACTCTACGAACAAAAAGAGGAATTCCAAATTCCGTAGCGATTTGTTT CTTGTACCTCTCTGCAGCAGCATGTGCAACTTCATGACAATCCACACAAAGAAGAACAATGTCA TGAGATCTATGACTTTTTAGGTGCTCAGGAAAATGAACTCTATAGCACGACGGTATTATTCGGT AACGGAGATAATGTTTCCCTTCCCCACACCCAACACATATATTCCTCTTGGTCTGGATATAAAA ATCGTTCCCCTCATCTTCTGGACGCCCTTTGGGCTCAAACAAAAGCATTATCGCAGGTGGATTT TCTTCAACTAGCTTCGCAAGGCCACGGTTTAGATAC
Genomic sequence	CTGTGACTAACATTCCAACCACCAGGTAAATGGCGAGGATGAACAGCATCAACAAACACTTTTC TCCACCTTTGACAGAAATCACGTACACCATCTTCTCCATGTTCCCTTAAAAGATATTCTACGAT CTGTTTCCCGTGCGGTCCATGTCCTAACAAAGAGAGTTTGGAGTTTTGTTGGTGCAACGTATTC CCATTACACTGAGTATCATTTAGCTCTTTAGCTCCATCTCCTTCACTTGTACCATCACCACTAT CATCAACAACTGTGCTCCTTTCTACAGTCATAATTTTCTCAAAATCTGCAAGAGCCTCCCCATC ATTGTTTTCATCTAAAGATACGAAGTTGTTTGCATCAGTATTTCTGGATATCAAAGGATCATCA TATCTTACACTTTATCAACCAACACAATTTTCAATGCTTATTATATGGTTACCTTCTTGTTTGT CCATACCAGCAACCTCTGCAGAATGTTTGAAGGAGACCCCTTTTTTCCTTTCGAGTTTTCTTCT TTCGTGAGGGCTCAATCCGATGAGTAAAGCTTTCTCTAAATCTTCTTCAGATAGGTCCCGTCCA CCGTAATACATCTTCACAGTCTATATTCAGAATCCAAAAGAAAGTATCAATAACAGAATACTTG GTTCAAATCAATTTCTTGAAAGTTGAAATAGTTAGATGATCACATCACCTGCAACAATTCCTCA CGGCGACTTGATGGCATTCTGTTCCCATGTCGCAAGAGCGCCATTGCAGCTGATCTAAGATGTA ATGGGGAGACACCTGCATCCTCAGAGTCACCAGTTGATTCATCCTCCACTAAAGAAGACGTCCC TTGTGCTTCTTTTGAATCGAGTACTCTACGAACAAAAAGAGGAATTCCAAATTCCGTAGCGATT TGTTTCTTGTACCTCTCTGCAGCAGCATGTGCAACTTCATGACAATCCACACAAAGAAGAACAA TGTCATGAGATCTATGACTTTTTAGGTGCTCAGGAAAATGAACTCTATAGCACGACGGTATTAT TCGGTAACGGAGATAATGTTTCCCTTCCCCACACCCAACACATATATTCCTCTTGGTCTGGATA TAAAAATCGTTCCCCTCATCTTCTGGACGCCCTTTGGGCTCAAACAAAAGCATTATCGCAGGTG GATTTTCTTCAACTAGCTTCGCAAGGCCACGGTTTAGATAC

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.21748111

Conservation Information
Conserved circRNAs	NA
PMCS	0.21748111

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							13762700-13761561(-)
						
						Potential amino acid sequence
						
							MLLFEPKGRPEDEGNDFYIQTKRNICVGCGEGKHYLRYRIIPSCYRVHFPEHLKSHRSHDIVLL
CVDCHEVAHAAAERYKKQIATEFGIPLFVRRVLDSKEAQGTSSLVEDESTGDSEDAGVSPLHLR
SAAMALLRHGNRMPSSRREELLQTVKMYYGGRDLSEEDLEKALLIGLSPHERRKLERKKGVSFK
HSAEVAGMDKQEDENNDGEALADFEKIMTVERSTVVDDSGDGTSEGDGAKELNDTQCNGNTLHQ
QNSKLSLLGHGPHGKQIVEYLLREHGEDGVRDFCQRWRKVFVDAVHPRHLPGGWNVSHRYLNRG
LAKLVEENPPAIMLLFEPKGRPEDEGNDFYIQTKRNICVGCGEGKHYLRYRIIPSCYRVHFPEH
LKSHRSHDIVLLCVDCHEVAHAAAERYKKQIATEFGIPLFVRRVLDSKEAQGTSSLVEDESTGD
SEDAGVSPLHLRSAAMALLRHGNRMPSSRREELLQTVKMYYGGRDLSEEDLEKALLIGLSPHER
RKLERKKGVSFKHSAEVAGMDKQEDENNDGEALADFEKIMTVERSTVVDDSGDGTSEGDGAKEL
NDTQCNGNTLHQQNSKLSLLGHGPHGKQIVEYLLREHGEDGVRDFCQRWRKVFVDAVHPRHLPG
GWNVSHRYLNRGLAKLVEENPPAIMLLFEPKGRPEDEGNDFYIQTKRNICVGCGEGKHYLRYRI
IPSCYRVHFPEHLKSHRSHDIVLLCVDCHEVAHAAAERYKKQIATEFGIPLFVRRVLDSKEAQG
TSSLVEDESTGDSEDAGVSPLHLRSAAMALLRHGNRMPSSRREELLQTVKMYYGGRDLSEEDLE
KALLIGLSPHERRKLERKKGVSFKHSAEVAGMDKQEDENNDGEALADFEKIMTVERSTVVDDSG
DGTSEGDGAKELNDTQCNGNTLHQQNSKLSLLGHGPHGKQIVEYLLREHGEDGVRDFCQRWRKV
FVDAVHPRHLPGGWNVSHRYLNRGLAKLVEENPPAIMLLFEPKGRPEDEGNDFYIQTKRNICVG
CGEGKHYLRYRIIPSCYRVHFPEHLKSHRSHDIVLLCVDCHEVAHAAAERYKKQIATEFGIPLF
VRRVLDSKEAQGTSSLVEDESTGDSEDAGVSPLHLRSAAMALLRHGNRMPSSRREELLQTVKMY
YGGRDLSEEDLEKALLIGLSPHERRKLERKKGVSFKHSAEVAGMDKQEDENNDGEALADFEKIM
TVERSTVVDDSGDGTSEGDGAKELNDTQCNGNTLHQQNSKLSLLGHGPHGKQIVEYLLREHGED
GVRDFCQRWRKVFVDAVHPRHLPGGWNVSH(-)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	13762700-13761561(-)
Potential amino acid sequence	MLLFEPKGRPEDEGNDFYIQTKRNICVGCGEGKHYLRYRIIPSCYRVHFPEHLKSHRSHDIVLL CVDCHEVAHAAAERYKKQIATEFGIPLFVRRVLDSKEAQGTSSLVEDESTGDSEDAGVSPLHLR SAAMALLRHGNRMPSSRREELLQTVKMYYGGRDLSEEDLEKALLIGLSPHERRKLERKKGVSFK HSAEVAGMDKQEDENNDGEALADFEKIMTVERSTVVDDSGDGTSEGDGAKELNDTQCNGNTLHQ QNSKLSLLGHGPHGKQIVEYLLREHGEDGVRDFCQRWRKVFVDAVHPRHLPGGWNVSHRYLNRG LAKLVEENPPAIMLLFEPKGRPEDEGNDFYIQTKRNICVGCGEGKHYLRYRIIPSCYRVHFPEH LKSHRSHDIVLLCVDCHEVAHAAAERYKKQIATEFGIPLFVRRVLDSKEAQGTSSLVEDESTGD SEDAGVSPLHLRSAAMALLRHGNRMPSSRREELLQTVKMYYGGRDLSEEDLEKALLIGLSPHER RKLERKKGVSFKHSAEVAGMDKQEDENNDGEALADFEKIMTVERSTVVDDSGDGTSEGDGAKEL NDTQCNGNTLHQQNSKLSLLGHGPHGKQIVEYLLREHGEDGVRDFCQRWRKVFVDAVHPRHLPG GWNVSHRYLNRGLAKLVEENPPAIMLLFEPKGRPEDEGNDFYIQTKRNICVGCGEGKHYLRYRI IPSCYRVHFPEHLKSHRSHDIVLLCVDCHEVAHAAAERYKKQIATEFGIPLFVRRVLDSKEAQG TSSLVEDESTGDSEDAGVSPLHLRSAAMALLRHGNRMPSSRREELLQTVKMYYGGRDLSEEDLE KALLIGLSPHERRKLERKKGVSFKHSAEVAGMDKQEDENNDGEALADFEKIMTVERSTVVDDSG DGTSEGDGAKELNDTQCNGNTLHQQNSKLSLLGHGPHGKQIVEYLLREHGEDGVRDFCQRWRKV FVDAVHPRHLPGGWNVSHRYLNRGLAKLVEENPPAIMLLFEPKGRPEDEGNDFYIQTKRNICVG CGEGKHYLRYRIIPSCYRVHFPEHLKSHRSHDIVLLCVDCHEVAHAAAERYKKQIATEFGIPLF VRRVLDSKEAQGTSSLVEDESTGDSEDAGVSPLHLRSAAMALLRHGNRMPSSRREELLQTVKMY YGGRDLSEEDLEKALLIGLSPHERRKLERKKGVSFKHSAEVAGMDKQEDENNDGEALADFEKIM TVERSTVVDDSGDGTSEGDGAKELNDTQCNGNTLHQQNSKLSLLGHGPHGKQIVEYLLREHGED GVRDFCQRWRKVFVDAVHPRHLPGGWNVSH(-)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Chu et al., 2017

circRNA

Detail information of AT2G32415_circ_g.2

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