Plant Circular RNA Database

Detail information of AT1G28520_circ_g.2

			
						 General Information
					
						CircRNA Name
						AT1G28520_circ_g.2
					
						ID in PlantcircBase
						ath_circ_004708
					
						Alias
						At_ciR418, AT1G28520_C1
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr1: 10029674-10029943  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							ue-circRNA							
									
						Identification method
						CIRCexplorer, PcircRNA_finder, circRNA_finder, find_circ, CIRI-full, CIRI2
					
						Parent gene
						
							AT1G28520						
					
						Parent gene annotation
						
							Transcription factor VOZ1
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT1G28520_circ_g.3 AT1G28520_circ_g.4 						
					
						Support reads
						31/13
					
						Tissues
						leaf/leaf, whole_plant
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT1G28520.5:1
AT1G28520.4:1
AT1G28520.1:1
AT1G28520.3:1
AT1G28520.2:1

General Information
CircRNA Name	AT1G28520_circ_g.2
ID in PlantcircBase	ath_circ_004708
Alias	At_ciR418, AT1G28520_C1
Organism	Arabidpsis thaliana
Position	chr1: 10029674-10029943 JBrowse»
Reference genome	TAIR10.38
Type	ue-circRNA
Identification method	CIRCexplorer, PcircRNA_finder, circRNA_finder, find_circ, CIRI-full, CIRI2
Parent gene	AT1G28520
Parent gene annotation	Transcription factor VOZ1
Parent gene strand	+
Alternative splicing	AT1G28520_circ_g.3 AT1G28520_circ_g.4
Support reads	31/13
Tissues	leaf/leaf, whole_plant
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT1G28520.5:1 AT1G28520.4:1 AT1G28520.1:1 AT1G28520.3:1 AT1G28520.2:1

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							GCCTACTGATGTTACTCTTCTTGAGGAGCAAGTGAATCAGATGCTTCGTGAGTGGAAATCTGAG
CTCAATGAGCCTTCTCCAGCTTCATCCTTGCAACAAagaatcttgtgtttttgtggtggggagg
tgagatttcttatgacggggaagcgatcaaagactaattgcagatctgcttctcacaagctctt
caaggata

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 227
Transcript exons: 10029674-10029722,10029766-10029943
AGAATCTTGTGTTTTTGTGGTGGGGAGGTGAGATTTCTTATGACGGGGACAAGGATAAGGCTAA
GAACCGTGTTGATGATTTGCAAGGCATGCTTTTGGATCTTCAGTTTGCGAGGAAAGAGAGTAGG
CCTACTGATGTTACTCTTCTTGAGGAGCAAGTGAATCAGATGCTTCGTGAGTGGAAATCTGAGC
TCAATGAGCCTTCTCCAGCTTCATCCTTGCAACAA
Transcript length: 270
Transcript exons: 10029674-10029943
AGAATCTTGTGTTTTTGTGGTGGGGAGGTGAGATTTCTTATGACGGGGAAGCGATCAAAGACTA
ATTGCAGATCTGCTTCTCACAAGCTCTTCAAGGATAAGGCTAAGAACCGTGTTGATGATTTGCA
AGGCATGCTTTTGGATCTTCAGTTTGCGAGGAAAGAGAGTAGGCCTACTGATGTTACTCTTCTT
GAGGAGCAAGTGAATCAGATGCTTCGTGAGTGGAAATCTGAGCTCAATGAGCCTTCTCCAGCTT
CATCCTTGCAACAA
						
						Genomic sequence
						
							AGAATCTTGTGTTTTTGTGGTGGGGAGGTGAGATTTCTTATGACGGGGAAGCGATCAAAGACTA
ATTGCAGATCTGCTTCTCACAAGCTCTTCAAGGATAAGGCTAAGAACCGTGTTGATGATTTGCA
AGGCATGCTTTTGGATCTTCAGTTTGCGAGGAAAGAGAGTAGGCCTACTGATGTTACTCTTCTT
GAGGAGCAAGTGAATCAGATGCTTCGTGAGTGGAAATCTGAGCTCAATGAGCCTTCTCCAGCTT
CATCCTTGCAACAA

Sequences
Splice junction sequence	GCCTACTGATGTTACTCTTCTTGAGGAGCAAGTGAATCAGATGCTTCGTGAGTGGAAATCTGAG CTCAATGAGCCTTCTCCAGCTTCATCCTTGCAACAAagaatcttgtgtttttgtggtggggagg tgagatttcttatgacggggaagcgatcaaagactaattgcagatctgcttctcacaagctctt caaggata
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 227 Transcript exons: 10029674-10029722,10029766-10029943 AGAATCTTGTGTTTTTGTGGTGGGGAGGTGAGATTTCTTATGACGGGGACAAGGATAAGGCTAA GAACCGTGTTGATGATTTGCAAGGCATGCTTTTGGATCTTCAGTTTGCGAGGAAAGAGAGTAGG CCTACTGATGTTACTCTTCTTGAGGAGCAAGTGAATCAGATGCTTCGTGAGTGGAAATCTGAGC TCAATGAGCCTTCTCCAGCTTCATCCTTGCAACAA Transcript length: 270 Transcript exons: 10029674-10029943 AGAATCTTGTGTTTTTGTGGTGGGGAGGTGAGATTTCTTATGACGGGGAAGCGATCAAAGACTA ATTGCAGATCTGCTTCTCACAAGCTCTTCAAGGATAAGGCTAAGAACCGTGTTGATGATTTGCA AGGCATGCTTTTGGATCTTCAGTTTGCGAGGAAAGAGAGTAGGCCTACTGATGTTACTCTTCTT GAGGAGCAAGTGAATCAGATGCTTCGTGAGTGGAAATCTGAGCTCAATGAGCCTTCTCCAGCTT CATCCTTGCAACAA
Genomic sequence	AGAATCTTGTGTTTTTGTGGTGGGGAGGTGAGATTTCTTATGACGGGGAAGCGATCAAAGACTA ATTGCAGATCTGCTTCTCACAAGCTCTTCAAGGATAAGGCTAAGAACCGTGTTGATGATTTGCA AGGCATGCTTTTGGATCTTCAGTTTGCGAGGAAAGAGAGTAGGCCTACTGATGTTACTCTTCTT GAGGAGCAAGTGAATCAGATGCTTCGTGAGTGGAAATCTGAGCTCAATGAGCCTTCTCCAGCTT CATCCTTGCAACAA

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.791283838

Conservation Information
Conserved circRNAs	NA
PMCS	0.791283838

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							10029713-10029940(+)
						
						Potential amino acid sequence
						
							MTGKRSKTNCRSASHKLFKDKAKNRVDDLQGMLLDLQFARKESRPTDVTLLEEQVNQMLREWKS
ELNEPSPASSLQQRILCFCGGEVRFLMTGKRSKTNCRSASHKLFKDKAKNRVDDLQGMLLDLQF
ARKESRPTDVTLLEEQVNQMLREWKSELNEPSPASSLQQRILCFCGGEVRFLMTGKRSKTNCRS
ASHKLFKDKAKNRVDDLQGMLLDLQFARKESRPTDVTLLEEQVNQMLREWKSELNEPSPASSLQ
QRILCFCGGEVRFLMTGKRSKTNCRSASHKLFKDKAKNRVDDLQGMLLDLQFARKESRPTDVTL
LEEQVNQMLREWKSELNEPSPASSLQQ(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						response to drought stress

Functional Information
Coding potential	Y
Potential coding position	10029713-10029940(+)
Potential amino acid sequence	MTGKRSKTNCRSASHKLFKDKAKNRVDDLQGMLLDLQFARKESRPTDVTLLEEQVNQMLREWKS ELNEPSPASSLQQRILCFCGGEVRFLMTGKRSKTNCRSASHKLFKDKAKNRVDDLQGMLLDLQF ARKESRPTDVTLLEEQVNQMLREWKSELNEPSPASSLQQRILCFCGGEVRFLMTGKRSKTNCRS ASHKLFKDKAKNRVDDLQGMLLDLQFARKESRPTDVTLLEEQVNQMLREWKSELNEPSPASSLQ QRILCFCGGEVRFLMTGKRSKTNCRSASHKLFKDKAKNRVDDLQGMLLDLQFARKESRPTDVTL LEEQVNQMLREWKSELNEPSPASSLQQ(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	response to drought stress

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017;Zhang et al., 2019

circRNA

Detail information of AT1G28520_circ_g.2

Address

Any questions?

Supported by