Plant Circular RNA Database

Detail information of Os02g0644000_circ_g.3

			
						 General Information
					
						CircRNA Name
						Os02g0644000_circ_g.3
					
						ID in PlantcircBase
						osa_circ_015767
					
						Alias
						Os_ciR2269
					
						Organism
						
							Oryza sativa						
					
						Position
						
							chr2: 25897918-25898456  JBrowse»						
					
						Reference genome
						
							IRGSP-1.0.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						CIRCexplorer, find_circ
					
						Parent gene
						
							Os02g0644100						
					
						Parent gene annotation
						
							Similar to Heat shock protein STI (Stress inducible protein) (Gm
STI). (Os02t0644100-01);Similar to heat shock protein STI. (Os02
t0644100-02)
							
						Parent gene strand
						+
					
						Alternative splicing
						
							Os02g0644000_circ_g.4 Os02g0644000_circ_g.5 Os02g0644000_circ_g.6 Os02g0644000_circ_g.7 Os02g0644000_circ_g.8 						
					
						Support reads
						5/1
					
						Tissues
						root/shoot, root
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						NA:0
Os02t0644100-01:2

General Information
CircRNA Name	Os02g0644000_circ_g.3
ID in PlantcircBase	osa_circ_015767
Alias	Os_ciR2269
Organism	Oryza sativa
Position	chr2: 25897918-25898456 JBrowse»
Reference genome	IRGSP-1.0.38
Type	e-circRNA
Identification method	CIRCexplorer, find_circ
Parent gene	Os02g0644100
Parent gene annotation	Similar to Heat shock protein STI (Stress inducible protein) (Gm STI). (Os02t0644100-01);Similar to heat shock protein STI. (Os02 t0644100-02)
Parent gene strand	+
Alternative splicing	Os02g0644000_circ_g.4 Os02g0644000_circ_g.5 Os02g0644000_circ_g.6 Os02g0644000_circ_g.7 Os02g0644000_circ_g.8
Support reads	5/1
Tissues	root/shoot, root
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	NA:0 Os02t0644100-01:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CTTATGGTTTGTAGGTAATCAGCTTTTCAAGGAGCAAAAATATCCAGACGCGGTAAAGCATTAC
ACTGAGGCTATCAGGAGAAATCCTAAGGACCCGAAGtacgatgaatgcattaaggactgtgata
aggctgtggagaggggaagggaacttcgtgctgatttcaagatgatctcaagggcactgacaag
aaaaggaa

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 375
Transcript exons: 25897918-25898206,25898371-25898456
TACGATGAATGCATTAAGGACTGTGATAAGGCTGTGGAGAGGGGAAGGGAACTTCGTGCTGATT
TCAAGATGATCTCAAGGGCACTGACAAGAAAAGGAACAGCCCTAGCCAAACTTGCTAAGACCTC
TAAGGACTATGACATTGCCATTGAGACTTTCCAGAAGGCTCTAACTGAGCATCGGAATCCAGAC
ACTCTAAAAAAGCTAAATGAGGCTGAGAGGGCAAAGAAAGAGTTGGAGCAACAAGAGTACTATG
ATCCAAAGTTAGCAGATGAAGAGAGAGAGAAAGGTAATCAGCTTTTCAAGGAGCAAAAATATCC
AGACGCGGTAAAGCATTACACTGAGGCTATCAGGAGAAATCCTAAGGACCCGAAG
						
						Genomic sequence
						
							TACGATGAATGCATTAAGGACTGTGATAAGGCTGTGGAGAGGGGAAGGGAACTTCGTGCTGATT
TCAAGATGATCTCAAGGGCACTGACAAGAAAAGGAACAGCCCTAGCCAAACTTGCTAAGACCTC
TAAGGACTATGACATTGCCATTGAGACTTTCCAGAAGGCTCTAACTGAGCATCGGAATCCAGAC
ACTCTAAAAAAGCTAAATGAGGCTGAGAGGGCAAAGAAAGAGTTGGAGCAACAAGAGTACTATG
ATCCAAAGTTAGCAGATGAAGAGAGAGAGAAAGGTATGGGATCCAGTAGGGTATCATGCTGTCA
TCTCTAGTTCTCCCCCCTGCTTTATTTATGTCCTCACCTGTCCCTTGCAGAGATTTCCCTTGCT
GCCATATTTTTTAGAGTTGCAATGTGCCATTTGTAGGTTGACTGGCTAGCTAATCCTTATGGTT
TGTAGGTAATCAGCTTTTCAAGGAGCAAAAATATCCAGACGCGGTAAAGCATTACACTGAGGCT
ATCAGGAGAAATCCTAAGGACCCGAAG

Sequences
Splice junction sequence	CTTATGGTTTGTAGGTAATCAGCTTTTCAAGGAGCAAAAATATCCAGACGCGGTAAAGCATTAC ACTGAGGCTATCAGGAGAAATCCTAAGGACCCGAAGtacgatgaatgcattaaggactgtgata aggctgtggagaggggaagggaacttcgtgctgatttcaagatgatctcaagggcactgacaag aaaaggaa
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 375 Transcript exons: 25897918-25898206,25898371-25898456 TACGATGAATGCATTAAGGACTGTGATAAGGCTGTGGAGAGGGGAAGGGAACTTCGTGCTGATT TCAAGATGATCTCAAGGGCACTGACAAGAAAAGGAACAGCCCTAGCCAAACTTGCTAAGACCTC TAAGGACTATGACATTGCCATTGAGACTTTCCAGAAGGCTCTAACTGAGCATCGGAATCCAGAC ACTCTAAAAAAGCTAAATGAGGCTGAGAGGGCAAAGAAAGAGTTGGAGCAACAAGAGTACTATG ATCCAAAGTTAGCAGATGAAGAGAGAGAGAAAGGTAATCAGCTTTTCAAGGAGCAAAAATATCC AGACGCGGTAAAGCATTACACTGAGGCTATCAGGAGAAATCCTAAGGACCCGAAG
Genomic sequence	TACGATGAATGCATTAAGGACTGTGATAAGGCTGTGGAGAGGGGAAGGGAACTTCGTGCTGATT TCAAGATGATCTCAAGGGCACTGACAAGAAAAGGAACAGCCCTAGCCAAACTTGCTAAGACCTC TAAGGACTATGACATTGCCATTGAGACTTTCCAGAAGGCTCTAACTGAGCATCGGAATCCAGAC ACTCTAAAAAAGCTAAATGAGGCTGAGAGGGCAAAGAAAGAGTTGGAGCAACAAGAGTACTATG ATCCAAAGTTAGCAGATGAAGAGAGAGAGAAAGGTATGGGATCCAGTAGGGTATCATGCTGTCA TCTCTAGTTCTCCCCCCTGCTTTATTTATGTCCTCACCTGTCCCTTGCAGAGATTTCCCTTGCT GCCATATTTTTTAGAGTTGCAATGTGCCATTTGTAGGTTGACTGGCTAGCTAATCCTTATGGTT TGTAGGTAATCAGCTTTTCAAGGAGCAAAAATATCCAGACGCGGTAAAGCATTACACTGAGGCT ATCAGGAGAAATCCTAAGGACCCGAAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							osi_circ_004139* osi_circ_012057 						
					
						PMCS
						0.510571923

Conservation Information
Conserved circRNAs	osi_circ_004139* osi_circ_012057
PMCS	0.510571923

			
						 Functional Information
					
						Coding potential
						Y
					
						Potential coding position
						
							25897988-25898453(+)
						
						Potential amino acid sequence
						
							MISRALTRKGTALAKLAKTSKDYDIAIETFQKALTEHRNPDTLKKLNEAERAKKELEQQEYYDP
KLADEEREKGNQLFKEQKYPDAVKHYTEAIRRNPKDPKYDECIKDCDKAVERGRELRADFKMIS
RALTRKGTALAKLAKTSKDYDIAIETFQKALTEHRNPDTLKKLNEAERAKKELEQQEYYDPKLA
DEEREKGNQLFKEQKYPDAVKHYTEAIRRNPKDPKYDECIKDCDKAVERGRELRADFKMISRAL
TRKGTALAKLAKTSKDYDIAIETFQKALTEHRNPDTLKKLNEAERAKKELEQQEYYDPKLADEE
REKGNQLFKEQKYPDAVKHYTEAIRRNPKDPKYDECIKDCDKAVERGRELRADFKMISRALTRK
GTALAKLAKTSKDYDIAIETFQKALTEHRNPDTLKKLNEAERAKKELEQQEYYDPKLADEEREK
GNQLFKEQKYPDAVKHYTEAIRRNPKDPK(+)
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						NA

Functional Information
Coding potential	Y
Potential coding position	25897988-25898453(+)
Potential amino acid sequence	MISRALTRKGTALAKLAKTSKDYDIAIETFQKALTEHRNPDTLKKLNEAERAKKELEQQEYYDP KLADEEREKGNQLFKEQKYPDAVKHYTEAIRRNPKDPKYDECIKDCDKAVERGRELRADFKMIS RALTRKGTALAKLAKTSKDYDIAIETFQKALTEHRNPDTLKKLNEAERAKKELEQQEYYDPKLA DEEREKGNQLFKEQKYPDAVKHYTEAIRRNPKDPKYDECIKDCDKAVERGRELRADFKMISRAL TRKGTALAKLAKTSKDYDIAIETFQKALTEHRNPDTLKKLNEAERAKKELEQQEYYDPKLADEE REKGNQLFKEQKYPDAVKHYTEAIRRNPKDPKYDECIKDCDKAVERGRELRADFKMISRALTRK GTALAKLAKTSKDYDIAIETFQKALTEHRNPDTLKKLNEAERAKKELEQQEYYDPKLADEEREK GNQLFKEQKYPDAVKHYTEAIRRNPKDPK(+)
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	NA

			
						 Other Information
					
						References
						Ye et al., 2015;Chu et al., 2017

circRNA

Detail information of Os02g0644000_circ_g.3

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