Plant Circular RNA Database

Detail information of AT5G32450_circ_g.4

			
						 General Information
					
						CircRNA Name
						AT5G32450_circ_g.4
					
						ID in PlantcircBase
						ath_circ_040956
					
						Alias
						At_ciR3200, AT5G32450_C2
					
						Organism
						
							Arabidpsis thaliana						
					
						Position
						
							chr5: 12080370-12080844  JBrowse»						
					
						Reference genome
						
							TAIR10.38						
					
						Type  
						
							e-circRNA							
									
						Identification method
						find_circ, CIRI2
					
						Parent gene
						
							AT5G32450						
					
						Parent gene annotation
						
							Putative RRM-containing protein
							
						Parent gene strand
						+
					
						Alternative splicing
						
							AT5G32450_circ_g.1 AT5G32450_circ_g.2 AT5G32450_circ_g.3 AT5G32450_circ_g.5 						
					
						Support reads
						2
					
						Tissues
						leaf
					
						Exon boundary  
						
							Yes-Yes							"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.

						Splicing signals  
						
							GT-AG							The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.

						Number of exons covered
						AT5G32450.1:2

General Information
CircRNA Name	AT5G32450_circ_g.4
ID in PlantcircBase	ath_circ_040956
Alias	At_ciR3200, AT5G32450_C2
Organism	Arabidpsis thaliana
Position	chr5: 12080370-12080844 JBrowse»
Reference genome	TAIR10.38
Type	e-circRNA
Identification method	find_circ, CIRI2
Parent gene	AT5G32450
Parent gene annotation	Putative RRM-containing protein
Parent gene strand	+
Alternative splicing	AT5G32450_circ_g.1 AT5G32450_circ_g.2 AT5G32450_circ_g.3 AT5G32450_circ_g.5
Support reads	2
Tissues	leaf
Exon boundary	Yes-Yes "Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
Splicing signals	GT-AG The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Number of exons covered	AT5G32450.1:2

			
						 Experimental Information
					
						Sanger sequencing for BSS  
						
							NA							Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".

						PCR primers for BSS   
						
							NA							PCR primers used in back-splicing site (BSS) validation.

						Sanger sequencing for FL   
						
							NA							Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.

						PCR primers for FL   
						
							NA							 PCR primers used in full-length (FL) sequence validation.

Experimental Information
Sanger sequencing for BSS	NA Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers for BSS	NA PCR primers used in back-splicing site (BSS) validation.
Sanger sequencing for FL	NA Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers for FL	NA PCR primers used in full-length (FL) sequence validation.

			
						 Sequences
					
						Splice junction sequence  
						
							CTGTTGATCAAAAGCTTCAAGTCTCCGATAAAACTATGGCAGCAATATTTGCAGCCGAGAGGAA
GTTAAACGACACGGGTTCAGCTGTCAAATCAAGCAGgaagtaagaatgttggacaacgcgatgc
ctcttggtcttcaagagagtactacacaggttaggagacgtgattttcatacagaaacttgtct
atgtgtga

						Assembled circRNA sequence  
						
							Yes
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).

						Full-length trsnacripts
						
							Transcript length: 398
Transcript exons: 12080370-12080426,12080504-12080844
GAAGTAAGAATGTTGGACAACGCGATGCCTCTTGGTCTTCAAGAGAGTACTACACAGACTAAAA
CGAACATGGATGGTAATAGTAGAGCATACGTTAGCAAAGCACAAGATGTGGTGGCAACGGTTTT
GGCAAAAGGGTCTGCTCTTGGACAAGATGCAGTGAATAAAGCCAAGGCTTTCGACGAAAAGCAC
CAATTAAGAGCCAATGCTTCTGCTAAAGTTTCATCTTTTGACAAGCGAGTTGGTCTTACTGAGA
AGTTATCTGTTGGTATTTCTGCTGTTAATGAGAAAGTTAAATCTGTTGATCAAAAGCTTCAAGT
CTCCGATAAAACTATGGCAGCAATATTTGCAGCCGAGAGGAAGTTAAACGACACGGGTTCAGCT
GTCAAATCAAGCAG
						
						Genomic sequence
						
							GAAGTAAGAATGTTGGACAACGCGATGCCTCTTGGTCTTCAAGAGAGTACTACACAGGTTAGGA
GACGTGATTTTCATACAGAAACTTGTCTATGTGTGATATAGTATAGTATTAAACATGTTGCCTT
GTGCAGACTAAAACGAACATGGATGGTAATAGTAGAGCATACGTTAGCAAAGCACAAGATGTGG
TGGCAACGGTTTTGGCAAAAGGGTCTGCTCTTGGACAAGATGCAGTGAATAAAGCCAAGGCTTT
CGACGAAAAGCACCAATTAAGAGCCAATGCTTCTGCTAAAGTTTCATCTTTTGACAAGCGAGTT
GGTCTTACTGAGAAGTTATCTGTTGGTATTTCTGCTGTTAATGAGAAAGTTAAATCTGTTGATC
AAAAGCTTCAAGTCTCCGATAAAACTATGGCAGCAATATTTGCAGCCGAGAGGAAGTTAAACGA
CACGGGTTCAGCTGTCAAATCAAGCAG

Sequences
Splice junction sequence	CTGTTGATCAAAAGCTTCAAGTCTCCGATAAAACTATGGCAGCAATATTTGCAGCCGAGAGGAA GTTAAACGACACGGGTTCAGCTGTCAAATCAAGCAGgaagtaagaatgttggacaacgcgatgc ctcttggtcttcaagagagtactacacaggttaggagacgtgattttcatacagaaacttgtct atgtgtga
Assembled circRNA sequence	Yes The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018) or CIRCexplorer2 (Zhang et al., 2016).
Full-length trsnacripts	Transcript length: 398 Transcript exons: 12080370-12080426,12080504-12080844 GAAGTAAGAATGTTGGACAACGCGATGCCTCTTGGTCTTCAAGAGAGTACTACACAGACTAAAA CGAACATGGATGGTAATAGTAGAGCATACGTTAGCAAAGCACAAGATGTGGTGGCAACGGTTTT GGCAAAAGGGTCTGCTCTTGGACAAGATGCAGTGAATAAAGCCAAGGCTTTCGACGAAAAGCAC CAATTAAGAGCCAATGCTTCTGCTAAAGTTTCATCTTTTGACAAGCGAGTTGGTCTTACTGAGA AGTTATCTGTTGGTATTTCTGCTGTTAATGAGAAAGTTAAATCTGTTGATCAAAAGCTTCAAGT CTCCGATAAAACTATGGCAGCAATATTTGCAGCCGAGAGGAAGTTAAACGACACGGGTTCAGCT GTCAAATCAAGCAG
Genomic sequence	GAAGTAAGAATGTTGGACAACGCGATGCCTCTTGGTCTTCAAGAGAGTACTACACAGGTTAGGA GACGTGATTTTCATACAGAAACTTGTCTATGTGTGATATAGTATAGTATTAAACATGTTGCCTT GTGCAGACTAAAACGAACATGGATGGTAATAGTAGAGCATACGTTAGCAAAGCACAAGATGTGG TGGCAACGGTTTTGGCAAAAGGGTCTGCTCTTGGACAAGATGCAGTGAATAAAGCCAAGGCTTT CGACGAAAAGCACCAATTAAGAGCCAATGCTTCTGCTAAAGTTTCATCTTTTGACAAGCGAGTT GGTCTTACTGAGAAGTTATCTGTTGGTATTTCTGCTGTTAATGAGAAAGTTAAATCTGTTGATC AAAAGCTTCAAGTCTCCGATAAAACTATGGCAGCAATATTTGCAGCCGAGAGGAAGTTAAACGA CACGGGTTCAGCTGTCAAATCAAGCAG

			
						 Conservation Information
					
						Conserved circRNAs
						
							NA						
					
						PMCS
						0.354779853

Conservation Information
Conserved circRNAs	NA
PMCS	0.354779853

			
						 Functional Information
					
						Coding potential
						N
					
						Potential coding position
						
							NA
						
						Potential amino acid sequence
						
							NA
						
						Sponge-miRNAs
						
							NA						
					
						circRNA-miRNA-mRNA network
						 VISUALIZATION
					
						Potential function description
						response to drought stress

Functional Information
Coding potential	N
Potential coding position	NA
Potential amino acid sequence	NA
Sponge-miRNAs	NA
circRNA-miRNA-mRNA network	VISUALIZATION
Potential function description	response to drought stress

			
						 Other Information
					
						References
						Ye et al., 2015;Zhang et al., 2019

circRNA

Detail information of AT5G32450_circ_g.4

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