circRNA ID
AT1G01320_circ_g.1
Alias
ath_circ_000020, At_ciR5170
Organism
Arabidpsis thaliana
Reference genome
TAIR10.38
Identification method
find_circ
Parent gene annotation
Tetratricopeptide repeat (TPR)-like superfamily protein
TGCGTGCAACTGCGTTGTTCACATGGTTAACAGAATCCATAACTTCATCGGCGACTGCCACATC
TTGCGTGCCTTCTTTGGTTTCTTCTACACCTGAAGTcaaactgattggcattctcagtgtttgt
ttcctgtttctcctccctcttaacaaaatgctgaaagttttctcaccttcctcagatttccggt
ttccattc
"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Sanger sequencing for BSS
NA
Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers used in back-splicing site (BSS) validation.
Assembled circRNA sequence
NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018).
Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers used in full-length (FL) sequence validation.
Genomic sequence
CAAACTGATTGGCATTCTCAGTGTTTGTTTCCTGTTTCTCCTCCCTCTTAACAAAATGCTGAAA
GTTTTCTCACCTTCCTCAGATTTCCGGTTTCCATTCTCCGTTTCACTGCGTGCAACTGCGTTGT
TCACATGGTTAACAGAATCCATAACTTCATCGGCGACTGCCACATCTTGCGTGCCTTCTTTGGT
TTCTTCTACACCTGAAGT
Number of exons covered
AT1G01320.3:1
AT1G01320.2:1
AT1G01320.1:1
Potential coding position
NA
Potential amino acid sequence
NA
circRNA-miRNA-mRNA network
References
Ye et al., 2015