circRNA ID
AT1G01110_circ_g.1
Alias
ath_circ_000012, Ath_circ_FC2512
Organism
Arabidpsis thaliana
Reference genome
TAIR10.38
Identification method
CIRCexplorer
Parent gene annotation
IQ-domain 18
ACGCTTGTCTCATGACGGTAGTCGCAAATCCGCGTTCAGTGACTCTCACGCTGTTTTTGAATCT
CGCTATCTTCAAGATTTGTCAGATCGACAATCCATGgcaagaagagcattaagagcattaaaag
ggttagtgaagctacaagcattggtgaggggacataatgtgagaaagcaagctaaaatgacatt
aaggtgta
"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Sanger sequencing for BSS
NA
Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers used in back-splicing site (BSS) validation.
Assembled circRNA sequence
NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018).
Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers used in full-length (FL) sequence validation.
Genomic sequence
GCAAGAAGAGCATTAAGAGCATTAAAAGGGTTAGTGAAGCTACAAGCATTGGTGAGGGGACATA
ATGTGAGAAAGCAAGCTAAAATGACATTAAGGTGTATGCAAGCTCTGGTTCGAGTCCAGTCTCG
TGTGCTTGACCAACGCAAACGCTTGTCTCATGACGGTAGTCGCAAATCCGCGTTCAGTGACTCT
CACGCTGTTTTTGAATCTCGCTATCTTCAAGATTTGTCAGATCGACAATCCATG
Number of exons covered
AT1G01110.1:1
AT1G01110.2:1
Potential coding position
53022-53180(+)
Potential amino acid sequence
MTLRCMQALVRVQSRVLDQRKRLSHDGSRKSAFSDSHAVFESRYLQDLSDRQSMARRALRALKG
LVKLQALVRGHNVRKQAKMTLRCMQALVRVQSRVLDQRKRLSHDGSRKSAFSDSHAVFESRYLQ
DLSDRQSMARRALRALKGLVKLQALVRGHNVRKQAKMTLRCMQALVRVQSRVLDQRKRLSHDGS
RKSAFSDSHAVFESRYLQDLSDRQSMARRALRALKGLVKLQALVRGHNVRKQAKMTLRCMQALV
RVQSRVLDQRKRLSHDGSRKSAFSDSHAVFESRYLQDLSDRQSM(+)
circRNA-miRNA-mRNA network
References
Chu et al., 2017;Chen et al., 2017