circRNA ID
AT1G01060_circ_g.5
Organism
Arabidpsis thaliana
Reference genome
TAIR10.38
Identification method
PcircRNA_finder
Parent gene annotation
LHY1
ACAAGTAAAGTATAAAAAAGAAACCATCTTTGATCTCCCCAAACATGAAAGTTTTCAAATTGCA
AAGCCGTTGTGATAACCTCTTTAGATTCTAGAGAAActtcaattcgttgccaagctcttccata
aagcctcaaggcttctagaaacctctcatgctcatcctcagtccatcgctctcgctgctttgtt
attgtata
"Exon boundary" shows whether the splicing sites are on the boundary of exons or not. For example, "Yes-No" represents the donor splicing site is on the exon boundary and the accepter is not on the exon boundary.
The splicing signals of the circRNA. For example, "AG-GT" represents that the donor and the accepter splicing signal is "AG" and "GT", respectively.
Sanger sequencing for BSS
NA
Sanger sequencing for back-splicing site (BSS) validation. "Yes" represents Sanger sequencing has been performed to valid the back-splicing site, "No" represents the opposite, "NA" represents "not available".
PCR primers used in back-splicing site (BSS) validation.
Assembled circRNA sequence
NA
The sequence of a circRNA based on assembly using circseq-cup (Ye et al., 2016) or CIRI-full (Zheng and Zhao, 2018).
Sanger sequencing for full-length (FL) sequence validation of circRNAs. "Yes" represents Sanger sequencing has been performed to valid the full-length of circRNA, "No" represents the opposite.
PCR primers used in full-length (FL) sequence validation.
Genomic sequence
CTTCAATTCGTTGCCAAGCTCTTCCATAAAGCCTCAAGGCTTCTAGAAACCTCTCATGCTCATC
CTCAGTCCATCGCTCTCGCTGCTTTGTTATTGTATATGGCTTTCTTGCCTAAACATTTGAAAAC
CCAAAAGAAAATCAAATCTTCACACAACAACAGTCCAAATCTCTCAAACAAAAAAACACCAATC
TTATTTCATTAGTAGTAGTACCTTAGCTAATAATTCTTCTCCAGATGTATTAGTATCCATAACA
GGACCGGTGCAGCTATTCGCTGCTTCAAATCCTCTCTAACAAGTAAAGTATAAAAAAGAAACCA
TCTTTGATCTCCCCAAACATGAAAGTTTTCAAATTGCAAAGCCGTTGTGATAACCTCTTTAGAT
TCTAGAGAAA
Number of exons covered
AT1G01060.2:2
AT1G01060.6:2
AT1G01060.7:2
AT1G01060.4:2
AT1G01060.1:2
AT1G01060.5:2
AT1G01060.3:2
AT1G01060.8:2
Potential coding position
NA
Potential amino acid sequence
NA
circRNA-miRNA-mRNA network
References
Chu et al., 2017